split gfp complementation assay for quantitative measurement of tau aggregation in situ
Báo cáo khoa học: " Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo" ppsx
... obtained from the breeding facility of the Institute of Poultry Sciences in Sichuan Agricultural University, China Animals were bred and maintained in an accredited facility at the Institute of ... Fifty goslings were randomly divided into groups In brief, a group of 40 goslings were orally infected with GPV CHV strain, using 0.1 mL of 103 LD50 per gosling Another group of 10 goslings was ... signal, suggesting that the established FQ-PCR assay was highly specific (Figure 2) The intraassay and interassay CV of this established FQPCR was in the range of 0.8-3% for most of the dynamic...
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Light sheet based fluorescence correlation and cross correlation spectroscopy for quantitative measurements of bio molecules in live cells
... players in biological systems to enable understanding in protein-protein interactions or protein-DNA interactions A number of techniques, including protein microarrays, pull down assays and chromatin ... CROSS-CORRELATION SPECTROSCOPY FOR QUANTITATIVE MEASUREMENTS OF BIO-MOLECULES IN LIVE CELLS ANAND PRATAP SINGH (M.Sc Chemistry, BHU, INDIA) NATIONAL UNIVERSITY OF SINGAPORE 2014 is Th pa ge in io nt te lly ... imaging method can provide spatio-temporal dynamics and binding maps of proteins localized in membrane, cytosol and nucleus This particular study combines fast SPIM imaging (single plane illumination...
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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens
... Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts in isolated ... ogttST::Cmr) strain (deficient in O6-methylguanine DNA methyltransferase) sensitive to alkylating chemicals These strains were kindly supplied by Dr T Nohmi of the National Institute of Health Sciences ... strains (Suzuki et al 1997, Yamada et al 1995) in order to assess the mutagenicity of 255 chemicals YG3003 was used to aid the identification of a variety of mutagenic free radicals including quinones,...
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Báo cáo khoa học: Quantitative modeling of triacylglycerol homeostasis in yeast – metabolic requirement for lipolysis to promote membrane lipid synthesis and cellular growth potx
... mobilization in yeast as sinks for internal fluxes From a biological point of view, they allow the cell to dispose of excess metabolites by storing them in an inert form When demand for these metabolites ... broken lines) TAG mobilization during pre-logarithmic growth as a function of time The thin dotted line represents a linear fit of the experimental data in the range 0.25–4 h The calculated lines ... hydrolysis of TAG or DAG, and the subsequent re-acylation of glycerol-3-phosphate (Fig 3) Interestingly, inactivation of DAG kinase activity resulted in a comparable TAG mobilization rate; in fact,...
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báo cáo sinh học:" Meeting human resources for health staffing goals by 2018: a quantitative analysis of policy options in Zambia" potx
... increase in the size of the training enrolment increase as it does for doctors At current levels of attrition, training enrolment must be increased by a factor of four (4×) for clinical officers, ... reach staffing targets by 2018 for doctors, clinical officers, or nurses, but they can reduce the size of the required increase in training enrolment, especially for doctors Increasing training enrolment ... component of voluntary attrition [26] Training parameters Training parameter estimates were derived from a 2008 joint Ministry of Health and Clinton Foundation assessment of all 39 medical training institutions...
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Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx
... existence of two distinct lineages of KSHV-like rhadinoviruses in Old World non-human primates [5,6] The rhadinovirus-1 (RV1) lineage includes KSHV and closely related homologs infecting different ... Research Center [18] In the Tulane study, a QPCR assay developed against the interleukin-6 homolog of RRV found infrequent and low levels of RRV in PBMC of healthy and SIVinfected rhesus macaques ... curve obtained with the RV2 rhadinovirus assay using RV2 DNA in a constant amount (1 µg) of genomic DNA DNA from MmuA01111 which was naturally infected with RRV was assayed in duplicate in four-fold...
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báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot
... existence of two distinct lineages of KSHV-like rhadinoviruses in Old World non-human primates [5,6] The rhadinovirus-1 (RV1) lineage includes KSHV and closely related homologs infecting different ... Research Center [18] In the Tulane study, a QPCR assay developed against the interleukin-6 homolog of RRV found infrequent and low levels of RRV in PBMC of healthy and SIVinfected rhesus macaques ... curve obtained with the RV2 rhadinovirus assay using RV2 DNA in a constant amount (1 µg) of genomic DNA DNA from MmuA01111 which was naturally infected with RRV was assayed in duplicate in four-fold...
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Báo cáo khoa học: " A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples" potx
... The assay was found to be effective in detecting as few as 10 organisms per 100 mg of feces This assay will be useful in identifying animals in their early clinical stage, shedding low numbers of ... RT- PCR product of RNA extracted from fecal samples containing 10 organisms of MAP RFR: reflectometer reading, CFU: colony forming units A biosensor assay for the detection of Mycobacterium ... containing mM EDTA) were diluted in hybridization buffer, and denatured o at 95 C for To this mixture, μl of liposomes for o each well was added and incubated at 60 C for 20 One hundred μl of this...
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Báo cáo khoa học: "A new experimental device for rapid measurement of the trunk equivalent modulus of elasticity on standing trees" potx
... relevance for measuring the mechanical properties of individual trees 2.1.1 The starting point: Koizumi’s method [14, 19] The principle of the MOE measurement method developed for standing trees ... the definitive apparatus [8] Cown D.J., Use of the Pilodyn wood tester for estimating wood density in standing trees – influence of site and tree age, Forest Research Institute, New Zealand Forest ... respectively) Measurement incertitude: Being given that the data involved in MOE calculation for metallic beams are at least 99% accurate, most of the incertitude that we observed was linked to the measurement...
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Báo cáo khoa học: "An experimental system for the quantitative C-labelling 14 of whole trees in situ" pps
... measuring the level of 14 still in the system at the end CO of the labelling period Before opening the chamber, they forced its atmosphere into a -trapping CO circuit generally containing KOH ... review of the constraints related to 14 labelling, and of the main C progress made in labelling chamber technology in order to meet them, particularly for trees, this paper presents a system allowing ... assuming equipressure between the inside of the chamber and atmosphere, when thermal dilatation of the air in the chamber occurs In such conditions, an increase in temperature of 15-20°C during...
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Báo cáo y học: "Reliability of the TekScan MatScan® system for the measurement of plantar forces and pressures during barefoot level walking in healthy adults" potx
... detailed information regarding the independent function of different segments of the foot We have previously used this mask to examine age-related changes in foot function [33], clinical predictors of ... differences in mean values as evidenced by paired t-tests for any of the regions assessed Discussion Information elicited from the analysis of plantar pressures and forces during walking can be an integral ... variables of interest by calculating CVs between three trials captured in a single session The intra-session CVs for the seven analysed regions ranged from to 22% for maximum force, to 32% for peak...
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Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf
... measure of the viral load in it Cut off value (RLU of specimen/ mean RLU of PC) was 1.0 pg/ml of sample Interpretation of Cut off value (RLU of specimen/mean RLU of PC) Cut-off ratio of to 0.99 ... http://www.virologyj.com/content/7/1/253 Page of 10 Figure Detection of HPV infection by PCR in OSMF and OSCC cases Gel figure A represent the ethidium bromide-staining in 2% agarose gel and showing presence of HPV infection in OSMF ... negative for HR HPV; Cutoff ratio greater than 1.0 positive for HR HPV The cut off ratio at 1.0 correspond to viral DNA load of 5,000 copies/ml of 1pg/ml at a threshold of finding a clinical disease...
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Báo cáo y học: " A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control" docx
... shows the performances of the duplex primer/probe (C) and singleplex primer/probe (A, B) assays in the testing of the same sample with of low load of HCV Assay of 109 serum samples by using commercial ... in Table 1, 1000 copies/ml of armored RNA was used as the optimal concentration of IC in the HCV RNA duplex realtime RT-PCR assay Intrinsic performance of the duplex real-time RT-PCR assay Linearity ... real-time RT-PCR assay (Table 4) Figure Linearity of the duplex real-time RT-PCR assay Linearity of the duplex real-time RT-PCR assay was determined using serial 10fold dilutions of a clinical sample...
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Báo cáo khoa học:" Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1?" pdf
... DNA amounts, eliminating the need for sample dilution and minimizing sample handling The results for intra- and inter -assay precision indicate that both intra -assay and inter -assay CVs were satisfactorily ... demonstrated that the established FQ-PCR assay is of highly specific The intra -assay and inter -assay CV of this established FQPCR was in the range of 1–3% for most of the dynamic range (from 1.0 × 109 ... subjected to initial denaturation at 95°C for min, followed by 50 cycles of 95°C for 60 s, annealing for 60 s, extension at 72°C for 60 s, and one cycle of final extension at 72°C for The amplified...
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Báo cáo khoa học: "Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA" ppt
... Ct/Cpvalue of 21.32 for the dilution of 10-2 and a Ct/Cp-value of 36.52 for a dilution factor 10-6 (fit point method) Linear regression plot showed a strong linear correlation between the obtained ... transcription-PCR assay Journal of Clinical Microbiology 2001, 39:4093-4096 Verstrepen WA, Bruynseels P, Mertens AH: Evaluation of a rapid real-time RT-PCR assay for the detection of enterovirus RNA in cerebrospinal ... a higher sensitivity of primer-probe set 2, we were not able to confirm this with Echovirus RNA Control We maintain primer-probe set in our optimized assay but in our opinion analysis with primer-probe...
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Báo cáo khoa học: "Verification of the Combimatrix influenza detection assay for the detection of influenza A subtype during the 2007–2008 influenza season in Toronto, Canada" pot
... analysis of influenza A is becoming increasingly prevalent in clinical microbiology laboratories, and is essential for public health surveillance of circulating strains, determination of annual vaccine ... city of the CombiMatrix influenza detection system for the detection of an influenza A subtype (either H1 or H3) was 100% The LOD of the Luminex RVP assay was found to be 0.3 TCID50s/mL for influenza ... H, Pauli G: Application of a fluorogenic PCR assay for typing and subtyping of influenza viruses in respiratory samples J Clin Microbiol 2000, 38:1552-1558 Poddar SK: Influenza virus types and...
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Báo cáo khoa học: " Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1" pdf
... DNA amounts, eliminating the need for sample dilution and minimizing sample handling The results for intra- and inter -assay precision indicate that both intra -assay and inter -assay CVs were satisfactorily ... demonstrated that the established FQ-PCR assay is of highly specific The intra -assay and inter -assay CV of this established FQPCR was in the range of 1–3% for most of the dynamic range (from 1.0 × 109 ... subjected to initial denaturation at 95°C for min, followed by 50 cycles of 95°C for 60 s, annealing for 60 s, extension at 72°C for 60 s, and one cycle of final extension at 72°C for The amplified...
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Báo cáo khoa học: "Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA" doc
... Ct/Cpvalue of 21.32 for the dilution of 10-2 and a Ct/Cp-value of 36.52 for a dilution factor 10-6 (fit point method) Linear regression plot showed a strong linear correlation between the obtained ... transcription-PCR assay Journal of Clinical Microbiology 2001, 39:4093-4096 Verstrepen WA, Bruynseels P, Mertens AH: Evaluation of a rapid real-time RT-PCR assay for the detection of enterovirus RNA in cerebrospinal ... a higher sensitivity of primer-probe set 2, we were not able to confirm this with Echovirus RNA Control We maintain primer-probe set in our optimized assay but in our opinion analysis with primer-probe...
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Báo cáo y học: "Electrical impedance tomography compared to positron emission tomography for the measurement of regional lung ventilation: an experimental study" pptx
... FL is an engineer in charge of the PET camera IF is a professor of physiology and was a visiting professor at the time of this experiment CG is a professor of critical care medicine and research ... non-uniformity distribution of errors in regional measurements was checked by inspecting plots of residuals vs predicted values The statistical analysis was performed using SPSS statistical software ... The results pertaining to ΔVAatten instead of VAatten were similar (not shown) Inspection of plots of residuals vs predicted values disclosed that errors in measurements were uniformly distributed...
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Báo cáo y học: "A classification based framework for quantitative description of large-scale microarray data" ppsx
... consistently well in identifying a subset of profiles (three out of seven profiles tested), but it did not identify the remaining profiles in which response was generated only for a part of the time ... reviews The genes involved in amino acid biosynthesis represent another interesting class in the kanamycin treatment When we searched this class for correlated profiles of subsets of genes, we observed ... profiles of top-scoring classes (Sij > 1) for drug treatments: (a) Kanamycin, (b) Novobiocin, (c) Norfloxacin treatment of the wild-type strain Classes are sorted from top to bottom in descending...
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