spermatogenesis dna repair poly adp ribose turnover the state of the art
Báo cáo khoa học: Poly(ADP-ribose) polymerase-1 protects excessive DNA strand breaks from deterioration during repair in human cell extracts pot
... of the repair reaction [poly( ADP- ribosyl)ation and dissociation from DNA] In contrast, the presence of other 2015 Role of PARP-1 in base excision repair J L Parsons et al A B C D Fig Repair of ... formation of poly( ADP- ribose) polymers in the absence of PARP-1 We thus conclude that the critical event in an in vitro BER reaction is poly( ADP- ribosyl)ation and dissociation of PARP-1, rather than ... PARP-1 then dissociates from DNA The release of automodified PARP-1 exposes the intermediate to Pol b and XRCC1 DNA ligase IIIa, which perform the remainder of the repair process If not enough repair...
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Tài liệu Báo cáo khoa học: Poly(ADP-ribose) The most elaborate metabolite of NAD+ Alexander Burkle pptx
... size Poly( ADP- ribose) polymerase family, member [PARP-1, ADPRT, ADPRT1, PARP, PPOL, pADPRT-1] Poly( ADP- ribose) polymerase family, member [PARP-2, NAD+ poly( ADP- ribose) polymerase-2] Poly( ADP- ribose) ... activity and is the only protein known to catalyse the hydrolysis of ADP- ribose polymers to free ADP- ribose (Fig 1) [43] Its products are free poly( ADP- ribose) and monomeric ADP- ribose, the latter ... activity regulates the DNA- binding activity of TRF2 via poly( ADP- ribosyl)ation of the dimerization domain of TRF2 as well as via noncovalent binding of poly( ADP- ribose) to the myb domain of TRF2 [102]...
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Báo cáo y học: "Small interfering RNA mediated Poly (ADP-ribose) Polymerase-1 inhibition upregulates the heat shock response in a murine fibroblast cell line" pptx
... inhibition of transcription Poly (ADP- ribosylation) of transcription factors prevents their binding to DNA On the contrary, inhibition of PARP-1 enables the binding of the transcription factors to their ... Szabo C: The therapeutic potential of poly( ADP- ribose) polymerase inhibitors Pharmacol Rev 2002, 54:375-429 Mota RA, Hernandez-Espinosa D, Galbis-Martinez L, et al: Poly( ADP- ribose) polymerase-1 ... role of poly( ADP- ribosyl) ation on core nucleosome structure J Biol Chem 1989, 264:8878-86 32 Ogata N, Ueda K, Kawaichi M, Hayaishi O: Poly( ADP- ribose) synthetase, a main acceptor of poly( ADP- ribose) ...
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Báo cáo y học: " Small interfering RNA mediated Poly (ADP-ribose) Polymerase-1 inhibition upregulates the heat shock response in a murine fibroblast cell line." pptx
... inhibition of transcription Poly (ADP- ribosylation) of transcription factors prevents their binding to DNA On the contrary, inhibition of PARP-1 enables the binding of the transcription factors to their ... Szabo C: The therapeutic potential of poly( ADP- ribose) polymerase inhibitors Pharmacol Rev 2002, 54:375-429 Mota RA, Hernandez-Espinosa D, Galbis-Martinez L, et al: Poly( ADP- ribose) polymerase-1 ... role of poly( ADP- ribosyl) ation on core nucleosome structure J Biol Chem 1989, 264:8878-86 32 Ogata N, Ueda K, Kawaichi M, Hayaishi O: Poly( ADP- ribose) synthetase, a main acceptor of poly( ADP- ribose) ...
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Báo cáo y học: " The role of poly (ADP-ribose) polymerase in ventilator-induced lung injury" ppsx
... Suh IB, Shin C, Shim JJ, In KH, Yoo SH, Kang KH: Inflammatory and transcriptional roles of poly (ADP- ribose) polymerase in ventilator-induced lung injury Crit Care 2008, 12:R108 Vaneker M, Halbertsma ... lungs, normal lungs are relatively insusceptible to the detrimental effects of mechanical ventilation [3] To investigate newer pathogenetic mechanisms of VILI with a normal lung model, a higher pressure ... Matthay MA: Pathogenetic significance of biological markers of ventilator-associated lung injury in experimental and clinical studies Chest 2006, 130:19061914 Page of (page number not for citation...
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Role of poly (ADP ribose) polymerase 1 and copper homeostasis factor, antioxidant protein 1 in the maintenance of genomic integrity
... replication 20 1.1.3.1 Poly (ADP- ribose) polymerase (PARP-1) Human poly (ADP- ribose) polymerase (PARP-1), a key player of the BER pathway, is a 113 kDa enzyme encoded by the Parp-1 gene on chromosome ... 1q41–42 PARP-1 is part of a family of enzymes that mediate poly (ADP- ribosyl)ation of proteins (Chambon et al., 1963) The catalytic centre in the C-terminus of PARP-1 termed the “PARP signature ... al., 2005) PARP-1 catalyses the synthesis of over 90 % of cellular poly (ADP- ribose) following DNA damage (Meyer-Ficca et al., 2005) PARP-1 is implicated during DNA repair as a recruiting molecule...
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Báo cáo khoa học: Effect of oxidative stress and involvement of poly(ADP-ribose) polymerase (PARP) in Dictyostelium discoideum development doc
... (ADP- ribose) polymerase and its cleavage in apoptosis J Biol Chem 273, 33533–33539 Hoger T, de Murcia MJ & de Murcia G (1999) PARP-2, a novel mammalian DNA damage-dependent poly( ADPribose) polymerase ... 1) The percentage of loose aggregates formed was also increased, whereas in the case of 5614 LA (h) 0.0 1.0 2.0 3.0 4.0 2.5 mM HA-5min To investigate the germination efficiency of spores and the ... (2005) Poly( ADP- ribose) polymerase activity prevents signaling pathways for cell cycle arrest after DNA methylating agent exposure J Biol Chem 280, 15773–15785 Virag L & Szabo C (2002) The therapeutic...
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báo cáo hóa học: " Poly(ADP-ribose)polymerase-1 modulates microglial responses to amyloid b" pot
... Immunostaining for poly( ADP- ribose) , the enzymatic product of PARP-1, showed poly( ADP- ribose) production within 60 minutes of Ab addition, blocked by PJ34 There was no poly( ADP- ribose) signal in PARP-1-/- ... 20 Chang WJ, Alvarez-Gonzalez R: The sequence-specific DNA binding of NFkappa B is reversibly regulated by the automodification reaction of poly (ADP- ribose) polymerase J Biol Chem 2001, 276:47664-47670 ... supported by the AHA (SDG 0835222N, TMK), the NIH (AG030207-A2, LG; AG029483 and NS041421, RAS) and by the Department of Veterans Affairs (RAS) Author details Department of Neurology, University of California,...
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Báo cáo y học: "Inflammatory and transcriptional roles of poly (ADP-ribose) polymerase in ventilator-induced lung injury" doc
... nicotinamide and ADP- ribose and then uses the latter to synthesize polymers of ADP- ribose in DNA repair [6] However, under conditions of severe DNA injury, overactivation of PARP severely depletes the intracellular ... TS: Poly( ADP- ribose) polymerase is involved in the development of diabetic retinopathy via regulation of nuclear factor-kappaB Diabetes 2004, 53:2960-2967 Szabo C, Dawson VL: Role of poly( ADP- ribose) ... mechanisms of the pathogenesis of VILI A clearer understanding of the action mechanisms of PARP and modulation of its effects may be clinically useful in the prevention and treatment of VILI in...
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Báo cáo khoa học: Cyclic ADP-ribose requires CD38 to regulate the release of ATP in visceral smooth muscle ppt
... no EFS eADPR for β-NAD + ADPR + cADPR eADPR for β-NAD + ADPR + cADPR Ado ADP eADPR for cADPR (1 nM) eADPR for cADPR (1 nM) ADP ATP Ado AMP 10 AMP cADPR, no EFS cADPR, no EFS 12 25 LU ADP 14 16 ... 3097 cADPR and CD38 modulate ATP release in the bladder B CD38+/+ A PS CD38–/– PS β-NAD + ADPR + cADPR β-NAD + ADPR + cADPR ADP Ado ADP ATP ST β-NAD + ADPR + cADPR ST Ado AMP ST, BoNTA β-NAD + ADPR ... absence of cADPR Note that the peak of eADPR (standing for b-NAD+ + ADPR + cADPR) was increased in the samples collected during superfusion with cADPR (Figs and 5), because the exogenous cADPR was...
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Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
... further our knowledge with respect to the mechanisms of action of these proteins, it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the structures of these ... clearly shown that the variation in the solvent-accessible area of the N-terminal part of XPCB–hHR23B is markedly higher than that of XPCB–hHR23A (Fig 5) The total surface area of all these structures ... analysis [22] to examine in more detail the flexible characteristics of the 275–284 segment of hHR23B Because of the high quality of our 15 N-HSQC spectra, 54 of the 58 protonated backbone nitrogen...
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Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx
... a polypeptide homologous to the DNA repair enzyme (DRT111) of Arabidopsis thaliana [9,10] Here, we report on the isolation of the gene locus, full-length cDNA and the functional analysis of the ... sequenced The nucleotide sequence analysis of the genomic DNA revealed the presence of two introns and three exons, which was confirmed by the isolation and sequencing of the fulllength TgDRE cDNA The ... comparison of cDNA and genomic DNA nucleotide sequences allowed the determination of the exact position of the two introns which were 780- and 630-bp long (Fig 1B) These introns contained the typical...
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Báo cáo khoa học: Kinetic study of the HIV)1 DNA 3¢-end processing Single-turnover property of integrase docx
... reason for the tight binding of the reaction product to the catalyst is not clear In the case of IN, tight binding of the protein to recessed viral DNA ends is obligatory in the context of the HIV ... two cases: either [IN]0 ) [DNA] 0 (single turnover) or [DNA] 0 ) [IN]0 (multiple turn- A Fig Simulation analysis of the processed DNA product formation and of the change in the INDNA substrate ... 0.06 Taking into account the standard duration of 3Â-processing assays, the absence of turnover in the case of IN originates primarily in the low kcat value The amount of INDNA complex, which did...
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Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot
... speculate that the release of DNA and TDP from the transition state complex might involve an activated water molecule in the active site Requirement of manganese as cofactor and the pH profile of TDP ... bond in the topoisomerase– DNA complex Hydrolysis of the phosphodiester bond in Production of recombinant human TDP variants Database searches of the human ortholog to the yeast TDP cDNA sequence ... monitored and the amount of released products was calculated based on the extinction coefficient (e) of 15 000 M)1Æcm)1 The rate was then determined as the amount of released p-nitrophenol per The pH...
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DNA REPAIR − ON THE PATHWAYS TO FIXING DNA DAMAGE AND ERRORS docx
... Families in DNA Repair 211 Francisco J Fernandez, Miguel Lopez-Estepa and M Cristina Vega Chapter 12 Mammalian Spermatogenesis, DNA Repair, Poly( ADP- ribose) Turnover: the State of the Art 235 Maria ... alters the cleavage pattern of flap DNA by Dna2 The average size of cleaved flaps is expanded in the presence of ATP (Bae et al., 2002) Furthermore, the addition of ATP allowed wild type Dna2 , ... been shown The current MMR model involves the recognition of the error-containing newly synthesized DNA strand through the presence of a gap, such as the end of the Okazaki fragment, and the directional...
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Báo cáo khoa học: "The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis" pdf
... agarose-encapsulated cells The range of DNA quantification, beginning just before the compression zone (CZ) and ending at the bottom of the smears, is shown (B) The amount of released DNA (optical density, ... ice for h and then at 37°C for 24 h At this point, due to the fragility of agarose plugs, the corners tended to break off easily Therefore, to ensure that the same number of cells (DNA) per lane ... without the interference of apoptosis On the other hand, the intensity of apoptosis could be easily estimated by subtracting the total DNA released in unirradiated zvd-treated cell-plugs from the...
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Báo cáo y học: "A general framework for quantifying the effects of DNA repair inhibitors on radiation sensitivity as a function of dose" pptx
... on survival and (3) the relative significance of these changes The main features of the method are the inclusion of an indicator term in the model to indicate the presence of the drug and a factor ... and the significance of terms in the model was tested using the log-likelihood ratio test This test considers the ratio of the likelihood of the model with the parameter to the model without the ... experiments, the merits of the approach were evaluated in terms of the capacity of the model to quantify and indicate the relative significance of the effects of PARP inhibition on the survival...
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THE INFLUENCE OF THE KU80 CARBOXY-TERMINUS ON ACTIVATION OF THE DNA-DEPENDENT PROTEIN KINASE AND DNA REPAIR IS DEPENDENT ON THE STRUCTURE OF DNA COFACTORS Derek S.
... S Woods The Influence of the Ku80 Carboxy-Terminus on Activation of the DNA- Dependent Protein Kinase and DNA Repair is Dependent on the Structure of DNA Cofactors In mammalian cells DNA double ... threatens the integrity of the genome In order to overcome these genomic stresses, DNA repair pathways have evolved to deal with a variety of DNA lesions Of these, DNA double strand breaks are a particularly ... in which the initial radical induced on the DNA produces a single strand break The radical is then transferred to the other strand causing the second break7 Of these two possibilities the latter...
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ELUCIDATING THE ROLE OF REDOX EFFECTS AND THE KU80 C-TERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU
... operation of each step of the pathway in order to optimize the method of manipulation The mechanisms of each step of the pathway have very specific functions and tend to be the more minute details The ... sufficient to bind DNA- PK (31-33) Upon removal of the C-terminus of Ku80 the kinase activity of DNA- PK is drastically decreased Due to the highly flexible nature of the C-terminus of Ku80 (3436), ... 1) The DNA dependent protein kinase catalytic subunit (DNA- PKcs) is then recruited to the site of a DSB through an interaction with both Ku and the DNA termini, thus generating the active DNA- PK...
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Turnover: The Real Bottom Line
... disruption of customer relations, the vacancy cost until the job is filled, costs resulting from disruption of the work flow, and the erosion of morale and stability of those who remain Further, there ... about themselves and their work, have a sense of purpose, and to be recognized when they their jobs well They want more than the standard pay and benefits package that formed the heart of traditional ... as others Managerial Style The experience, background, and training of managers appear to have a significant impact on the problem of turnover Studies show that the backgrounds of managers profoundly...
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