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Báo cáo y học: "Prostaglandin E2 synthesis in cartilage explants under compression: mPGES-1 is a mechanosensitive gene" docx
... time, its expression is described in both canine and human cortex and aorta, and in the rodent heart, kidney and neuronal tissues [18] Prostaglandin endeperoxyde H2 is subsequently metabolized ... the primary antibody in the diluant solution (BSA 1% and goat serum 10% for PGES, and BSA 10% and milk 1% for COX-2) Immunohistological analysis was carried out on two uncompressed and two compressed ... increased COX-2 gene expression after h and h in compressed explants versus control and no increase for COX-1 and COX-3 Standard curves for COX-1, COX-2, COX-3 and hypoxanthine-guanine phosphoribosyltransferase...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
... 18sRNA and BACT were consistently unreliable and should be used with caution in studies involving virally infected cells Materials and methods Virus culture HSV-1 strain NC1, CMV strains Towne and ... Toledo, VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage of cells infected ... CB and AC conceived of the study AH designed and supervised the experiments, prepared the HIV infected MDDC extracts, and prepared the manuscript with CB SW carried out the QPCR experiments and...
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Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection ... http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence and real-time PCR [8-11] For kinetic ... viral RNA was performed to proof and monitor infection Thereafter the candidate reference genes were evaluated by the BestKeeper tool [6], the GeNorm tool [7] and the algorithm we described previously...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
... 18sRNA and BACT were consistently unreliable and should be used with caution in studies involving virally infected cells Materials and methods Virus culture HSV-1 strain NC1, CMV strains Towne and ... Toledo, VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage of cells infected ... CB and AC conceived of the study AH designed and supervised the experiments, prepared the HIV infected MDDC extracts, and prepared the manuscript with CB SW carried out the QPCR experiments and...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection ... http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence and real-time PCR [8-11] For kinetic ... viral RNA was performed to proof and monitor infection Thereafter the candidate reference genes were evaluated by the BestKeeper tool [6], the GeNorm tool [7] and the algorithm we described previously...
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báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt
... differences in the expression profile between sexual and apomictic plants must occur, from both sexual and apomictic accessions Results and discussion Candidate reference genes In order to pinpoint ... which were ubiquitinconjugating enzymes (BbrizUBCE1 and BbrizUBCE2) and elongation factor-1 alpha (BbrizEF1), and showed the narrowest Ct range and therefore the least deviation from the Ct median ... spikelets and ovary tissues in the four developmental stages in both apomictic and sexual B brizantha was calculated (additional file 2) BbrizUBCE and BbrizTUB were again the more stable and the...
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báo cáo khoa học: " Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida" pot
... RPS13 CYP RPS13 SAND TUB SAND TUB EF1alpha SAND GAPDH RPS13 UBQ TUB UBQ UBQ EF1alpha TUB GAPDH TUB RAN1 RAN1 ACT SAND ACT GAPDH ACT SAND ACT SAND RAN1 CYP GAPDH TUB GAPDH GAPDH GAPDH SAND GAPDH GAPDH ... JW and MEC designed the experiments IM and SL performed the experiments IM performed data analysis and table and figure drawing MEC wrote the first draft, and IM, BH, SL, JW and MEC corrected and ... in Mitchell are EF1a, SAND and RPS13; the three showing the lowest reliability are TUB, ACT and GAPDH (Figure 6A and 6B) For V30, the best candidate genes are CYP, RAN1 and ACT, while the three...
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Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps
... combination of primers, probe and annealing temperature was used for subsequent experiments Standard curve establishment The amplification curves (Figure 1.a.) and standard curve (Figure 1.b.) of ... changes and serological methods [45-47] However, these methods are time-consuming, inconvenient, and requiring special collection and transport conditions to maintain the viability of the virus, and ... PCR and has being become an increasingly popular way for the diagnosis of bacteria and viruses infection The diagnostic process requires only hours for detection and quantitation of bacteria and...
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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
... AD, and SC validation lines, 92% (11/12) for the SQ line, 77% (10/13) for the LC line, and 92% (44/48) overall CSTA, DUSP4, and S100P were upregulated consistently in only AD cells, and FOSL1 and ... correlated with the expression of RAB25 and AMY2A, and positively correlated with TGM2, MALL, and IGSF3 Gene networks and gene pathways To further understand the biological networks of the 19 genes, ... CA, USA), pmol of each forward and reverse primer, and a cDNA template corresponding to 400 pg total RNA The primer sequences and Ct values of the 19 candidate genes and GAPDH (a housekeeping gene...
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Báo cáo hóa học: " A quantitative real time PCR method to analyze T cell receptor Vb subgroup expansion by staphylococcal superantigens" doc
... were observed for some SAgs such as SEA (Vb15, 22, and 24), SEB (Vb 14), SED (Vb3, 9, and 14), SEE (Vb9 and 16), SEG (Vb15), and SElN (Vb7, 8, and 17) Also, some Vb previously reported specificities ... using this method Materials and methods Toxin production and purification SEB, SEC1 and TSST-1 were purified from cultures of S aureus MNHOCH, S aureus RN4220 (pMIN121) and S aureus RN4220 (pCE107), ... among triplicate reactions (Figure 1A) The slope and correlation coefficient (R2) of standard curves are used to determine primer efficiency and standard curve validity, respectively Results obtained...
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Báo cáo sinh học: "Quantitative real-time PCR study on persistence of pDNA vaccine pVax-Hsp60 TM814 in beef muscles" pptx
... J, Durland R, Coleman M: Biodistribution and safety studies of hDel-1 plasmidbased gene therapy in mouse and rabbit models Journal of Pharmacy and Pharmacology 2004, 56:177-185 Mena A, Andrew ... β-galactosidase activity and persistence of fluorescent liposome- pLacZ complexes in mice calf muscles Mice calf muscles were histochemically stained for β-galactosidase activity at the day (A) and at ... regulatory authorities will demand a reliable, sensitive and cost effective method for the determination of the amount of residual plasmid and its localization in the body at the time of the slaughter...
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Quantitative real time PCR
... amount of RNA and reaction volume, priming oligonucleotide if using gene-specific priming and concentration, temperature, and time) and three desirable (manufacturer of reagents and catalogue ... Calculate the standard deviation per microRNA across all tested samples 26 Jan Hellemans and Jo Vandesompele Select candidate microRNAs that (a) have data for all samples, (b) have lowest standard deviation, ... Viral/Pathogen Detection and Quantitation [ 3–5] Currently the quantitation of viruses and pathogens requires highly defined standards, and assays must be performed under identical conditions and at the same...
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Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
... -2, and -3 in the synovium synthase-1, 2, and -3 and (OA) levels of the messages (RA) Relative expressionand rheumatoid arthritis for hyaluronanof knees in 2, and -3 and hyaluronidase-1, -2, and ... absorb and digest hyaluronan, because type A cells have many vesicles and vacuoles containing lysosomal enzyme – such as nonspecific esterase, acid phosphatase, and cathepsins B, D, and L – and ... patients with OA or RA and for control donors from whom synovial samples were obtained are summarized in Table Two of us (MY and SS), both physicians, clinically diagnosed OA and diagnosed RA according...
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Báo cáo hóa học: "Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma" potx
... of the study FA and WDY drafted the manuscript and participated in the statistical analysis HP and XRH conceived of the study, and participated in its design and coordination and helped to draft ... aberrations [5,6] and that they grow ex vivo as cell lines with a malignant phenotype [7] Several approaches to detect CTCs have been described and can be classified into PCR-based methods and cytometric ... transferred into a 30mL falcon tube and centrifuged at 1,800 rpm at room temperature for 20 minutes Serum was removed, and cells were resuspended in mL saline and 0.3 mL RNA later solution After...
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Báo cáo sinh học: " Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" potx
... was designed by MJH and EANA; experimental work was done by EANA, MJH and AN; interpretation and laboratory work was conducted by MJH, EANA, AN, SK and RB; EANA, RLB, SK and MJH were responsible ... ABI sequence detection system included standards of 106 to 10 copies per reaction and negative controls These were used to generate a standard curve and calculate the copy number of the unknown ... type specific antibody reactivity or unique region sequence data Further data need to be gathered to evaluate the usefulness of these methods and their application to population and epidemiological...
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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
... 350714 and Y = × 106X + × 107 and the r2 were 1.0 and 0.95 for JCV(Mad1) virus stocks I and II, respectively Page of (page number not for citation purposes) Virology Journal 2006, 3:3 and inter-run ... employing microtiter and transwell plates Semiquantitative polymerase chain reaction (PCR) and quantitative real-time PCR have been recently developed and employed for the detection and quantitation ... blood-brain-barrier and lytic infection of oligodendrocytes, are still unclear Efforts to understand the pathogenesis of PML have been hampered by the lack of standard methods for JCV detection and quantitation...
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báo cáo hóa học:" Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" pdf
... was designed by MJH and EANA; experimental work was done by EANA, MJH and AN; interpretation and laboratory work was conducted by MJH, EANA, AN, SK and RB; EANA, RLB, SK and MJH were responsible ... ABI sequence detection system included standards of 106 to 10 copies per reaction and negative controls These were used to generate a standard curve and calculate the copy number of the unknown ... type specific antibody reactivity or unique region sequence data Further data need to be gathered to evaluate the usefulness of these methods and their application to population and epidemiological...
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báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx
... 350714 and Y = × 106X + × 107 and the r2 were 1.0 and 0.95 for JCV(Mad1) virus stocks I and II, respectively Page of (page number not for citation purposes) Virology Journal 2006, 3:3 and inter-run ... employing microtiter and transwell plates Semiquantitative polymerase chain reaction (PCR) and quantitative real-time PCR have been recently developed and employed for the detection and quantitation ... blood-brain-barrier and lytic infection of oligodendrocytes, are still unclear Efforts to understand the pathogenesis of PML have been hampered by the lack of standard methods for JCV detection and quantitation...
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Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps
... 3.57 ± 0.02 and 3.57 ± 0.03 log10 CFU/ml for S Typhimurium and S Enteritidis in beef, and 4.57 ± 0.02 and 2.26 ± 0.05 log10 CFU/ml for S Typhimurium and S Enteritidis in pork (Figs and 4) Our ... lysis method were 3.57 ± 0.03 and 4.56 ± 0.03 log10 CFU/ml for S Typhimurium and S Enteritidis in beef, and 4.57 ± 0.02 and 2.26 ± 0.05 log10 CFU/ml for S Typhimurium and S Enteritidis in pork The ... Detection limit and standard curve of the multiplex real-time PCR The detection limit and standard curve of the multiplex real-time PCR was determined using S Typhimurium ATCC 14028 and S Enteritidis...
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