single nucleotide polymorphism analysis by maldi tof mass spectrometry
Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... comparison of the unfolding transitions induced by temperature Cytotoxicity assays MALDI- TOF MS All mass spectra were acquired on a Bruker ULTRAFLEX ` TOF mass spectrometer, at the Servei de Proteomica ... (M23L) by the addition of AAP, as described in the Materials and methods Completion of the hydrolysis was confirmed by the disappearance of the (Met1)-ONC (M23L) signal in the MALDI- TOF mass spectra ... methods for the production and purification of the enzyme [13] The molecular mass of each purified product, as measured by MALDI- TOF MS, was 11 947.87±2 for (Met1)-ONC (M23L) and 11 838.21±2 for rONC,...
- 9
- 704
- 0
Báo cáo y học: "Translational Medicine and Reliability of Single-Nucleotide Polymorphism Studies: Can We Believe in SNP Reports or Not"
... (57.5) 1-3 polymorphisms tested 13 (48) 14 (52) > polymorphisms tested (7) 25 (93) 1-3 polymorphisms tested (19) 17 (81) > polymorphisms tested (24) 19 (76) 1-3 polymorphisms tested > polymorphisms ... according to number of polymorphisms tested Polymorphisms No of studies (%) P-value Positive outcome (%) Negative outcome (%) 1-3 polymorphisms tested 29 (74) 10 (26) > polymorphisms tested 17 ... studies analysing a determined polymorphisms between the studies examining it within 1-3 polymorphisms versus studies examining the same polymorphism among more than polymorphism Since the distribution...
- 9
- 524
- 0
Tài liệu Báo cáo khoa học: Fra-1 targets the AP-1 site/2G single nucleotide polymorphism (ETS site) in the MMP-1 promoter docx
... inhibited by 50% and the 2G allele by 50–70% [15] Thus, the effects of decreasing Fra-1 expression mimic the effects of inhibition by PD098059, and the reduction in Fra-1 expression by the addition ... activity by fibroblast growth factor Eur J Biochem 247, 503–510 20 Rutter, J.L., Mitchell, T.I., Buttice, G., Meyers, J., Gusella, J.F., Ozelius, L.J & Brinckerhoff, C.E (1998) A single nucleotide polymorphism ... promoter polymorphism 1G/2G is correlated with colorectal cancer invasiveness Clin Cancer Res 7, 2344–2346 Zhu, Y., Spitz, M.R., Lei, L., Mills, G.B & Wu, X (2001) A single nucleotide polymorphism...
- 10
- 406
- 0
Báo cáo khoa học: Formation of nucleoprotein RecA filament on single-stranded DNA Analysis by stepwise increase in ligand complexity potx
... various enzymes with mononucleotides of d(pC)n, d(pT)n, d(pG)n and d(pA)n are additive and elongation of these d(pN)n by one nucleotide unit results in an increase in the affinity by a factor f of 1.4–2.0 ... values were determined by extrapolation of lg dependencies to n ¼ or n ¼ 1, respectively (Fig 2; Table 1) Interestingly, the affinity of a single internucleoside phosphate or a single d(pN) unit of ... nonspecific oligonucleotides Bioorg Khim (Mosc) 29, 249–262 Bianco PR & Weinstock GM (1996) Interaction of the RecA protein of Escherichia coli with single- stranded oligodeoxyribonucleotides Nucleic...
- 12
- 378
- 0
Analysis of keto enol tautomers of curcumin by liquid chromatography mass spectrometry
... N.E Jacobsen, B.N Timmermann, D.R Gang, Analysis of curcuminoids by positive and negative electrospray ionization and tandem mass spectrometry, Rapid Commun Mass Spectrom 20 (2006) 1001–1012 [18] ... Fig The results by LC/UV and LC/MS/MS indicate that the major form of curcumin in water/acetonitrile is enol form Conclusion Our experiment with a PDA detector and a QIT /TOF mass spectrometer ... of enol In negative detection, mass spectra (Fig 3b and d) were similar to those without use of deuterium (Fig 2b and d) This indicates that major product ions by negative MS/MS not contain exchangeable...
- 3
- 437
- 0
Báo cáo y học: "Association of PTPN22 1858 single-nucleotide polymorphism with rheumatoid arthritis in a German cohort: higher frequency of the risk allele in male compared to female patients" potx
... C1858T single- nucleotide polymorphism with rheumatoid arthritis phenotypes in an inception cohort Arthritis Rheum 2005, 52:2948-2950 Viken MK, Amundsen SS, Kvien TK, Boberg KM, Gilboe IM, Lilleby ... performed by oligonucleotide hybridization of the PCR products to probes specific for DRB1*01 through *18 Hybridization was performed in a dot-blot format with digoxigenin-11-ddUTP-labeled oligonucleotides ... Chokkalingam AP, Alexander HC, Ardlie KG, Huang Q, Smith AM, Spoerke JM, et al.: A missense single- nucleotide polymorphism in a gene encoding a protein tyrosine phosphatase (PTPN22) is associated with...
- 7
- 420
- 0
Báo cáo y học: "A case-control study of rheumatoid arthritis identifies an associated single nucleotide polymorphism in the NCF4 gene, supporting a role for the NADPH-oxidase complex in autoimmunity" doc
... genotype frequencies and number (in brackets) Haplotype analysis reveals an associated haplotype in NCF4 caused by the rs729749 single nucleotide polymorphism We wished to estimate haplotypes and haplotype ... could be an important regulator of the immune system Materials and methods Selection of single nucleotide polymorphism The HapMap genome browser [32] was used as the primary source for selection ... stratified analysis, the Bonferroni correction method was applied, as implemented on the simple interactive statistical analysis website [35] We also performed logistic regression analysis for...
- 11
- 475
- 0
Báo cáo y học: "Association of a single nucleotide polymorphism in growth differentiate factor 5 with congenital dysplasia of the hip: a case-control study" ppt
... disorder caused by GDF5 mutation [16,17], and some patients with type C brachydactyly also present with dysplasia of hip joints [18,19] Recently, a functional single nucleotide polymorphism (SNP) ... differentiate factor 5; SNP = single nucleotide polymorphism Page of (page number not for citation purposes) Arthritis Research & Therapy Vol 10 No Dai et al Table Association of C/T polymorphism of the ... Control CDH = congenital dysplasia of the hip; GDF5 = growth differentiate factor 5; SNP = single nucleotide polymorphism may be due to the limited sample number, although a large sex bias of CDH...
- 5
- 443
- 0
Báo cáo y học: "A statistical approach for detecting genomic aberrations in heterogeneous tumor samples from single nucleotide polymorphism genotyping data" pot
... GC, Ogawa S: A robust algorithm for copy number detection using high-density oligonucleotide single nucleotide polymorphism genotyping arrays Cancer Res 2005, 65:6071-6079 Komura D, Shen F, Ishikawa ... Hudson TJ, Johnson BE, Lander ES, Meyerson M: Loss-of-heterozygosity analysis of small-cell lung carcinomas using single- nucleotide polymorphism arrays Nat Biotechnol 2000, 18:1001-1005 Zhao X, Li ... genomewide detection of allelic composition in nonpaired, primary tumor specimens by use of affymetrix single- nucleotide- polymorphism genotyping microarrays Am J Hum Genet 2007, 81:114-126 28 Yau C,...
- 15
- 583
- 0
báo cáo khoa học: "RRM1 single nucleotide polymorphism -37C®A correlates with progression-free survival in NSCLC patients after gemcitabine-based chemotherapy" potx
... selected the polymorphism sites -37C®A, 2455A®G, and 2464G®A as target SNPs The RRM1 polymorphism C(-)37A affects promoter activity in vitro[19] but the use of a single genetic polymorphism, ... gemcitabine Blood samples from 56 patients were available for the analysis of RRM1 SNPs An analysis of sequence chromatograms revealed RRM1 polymorphisms (Fig 2) The allele Dong et al Journal of Hematology ... cancer according to promoter polymorphisms of the ribonucleotide reductase M1 gene Clin Cancer Res 2008, 14:3083-3088 Sarries C, Alberola V, De Las Peñas A, Camps C, Massuti B, Garcia-Gomez R,...
- 8
- 222
- 0
Báo cáo y học: "Association of the T allele of an intronic single nucleotide polymorphism in the colony stimulating factor 1 receptor with Crohn''''s disease: a case-control study" ppt
... and ulcerative colitis susceptibility is the multidrug resistance transporter (MDR1) A single nucleotide polymorphism (SNP) in the coding region of the gene (Ala893Ser/Thr) has been found to occur ... design and analysis EM conceived of the study, recruited patients, performed the data analysis and drafted the manuscript 10 11 12 13 14 Acknowledgments This work was sponsored in part by grants ... arthritis, an autoimmune disease that sometime occurs in individuals and families affected by Crohn's disease [12] Polymorphisms in the promoter of the CD14 gene, which plays a critical role in lipopolysaccharide...
- 8
- 294
- 0
báo cáo khoa học: " Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour" ppt
... molecular weight; LMW: low molecular weight; MALDI- TOF: matrix-assisted laser desorption/ionization time of flight mass spectrometry; MS/MS: tandem mass spectrometry; MW: molecular weight; NCBI: ... C-type low molecular weight glutenin subunits by electrospray ionization mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry Proteomics 2005, 5:719-728 27 Ferrante ... expressed in this cultivar by analysis of publicly available EST data In addition to eliminating the inherent bias that results from amplification of sequences by PCR, the analysis provides new information...
- 14
- 325
- 0
Báo cáo y học: "Natural selection of protein structural and functional properties: a single nucleotide polymorphism perspect" potx
... Man; SNP, single nucleotide polymorphism Authors' contributions ZZ and JL designed the study JL and YZ collected the data and performed the data analysis XL participated in the statistical analysis ... version 3.0 [49], transmembrane helices by TMHMM 2.0 [50], secondary structures by PSIPRED [41], sequence complexity by SEG [46], and natively disordered proteins by Disopred2 [43] Disopred2 predictions ... structure features by the following computational methods using their default parameters: two-state (exposed or buried) solvent accessibility by PROFacc [40], signal peptides by SignalP version...
- 17
- 312
- 0
Báo cáo y học: "Whole genome sequencing of a single Bos taurus animal for single nucleotide polymorphism discovery" potx
... only the reference allele Table SNPs called by MAQ compared with calls by MALDI- TOF genotyping Concordant calls MAQ heterozygote under-call 186 MALDI- TOF homozygous, MAQ heterozygous Error rate ... with MALDI- TOF genotypes resulted in a false-positive rate as high as 24% (18 of 75) All these SNPs were called as heterozygotes by MAQ Of these SNPs, 11 were called as homozygotes by MALDI- TOF ... insertion/deletion event; MAF: minor allele frequency; MALDI- TOF: matrix-assisted laser desorption/ionization time-of-flight; SNP: single nucleotide polymorphism Analysis software We used the bosTau4.0 assembly...
- 8
- 241
- 0
Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx
... ionization Fourier-Transform mass spectrometry Nat Prod Rep 23, 893–918 35 Bumpus SB & Kelleher NL (2008) Accessing natural product biosynthetic processes by mass spectrometry Curr Opin Chem Biol ... biosynthesis by Fourier Transform mass spectrometry: The polyketide module of yersiniabactin synthetase Biochemistry 42, 13393–13400 33 Hicks LM, O’Connor SE, Mazur MT, Walsh CT & Kelleher NL (2004) Mass ... electrospray mass spectrometry in enzymic studies on acyl carrier protein implicated in fatty acid biosynthesis in Saccharopolyspora erythraea Chem Commun 11, 778–779 29 Hitchman TS, Crosby J, Byrom...
- 13
- 426
- 0
Báo cáo khoa học: "Simultaneous Determination of Various Macrolides by Liquid Chromatography/Mass Spectrometry" pps
... z 576.5 and 679.5 in mass spectra of erythromycin and roxithromycin were corresponding with Simultaneous Determination of Various Macrolides by Liquid Chromatography /Mass Spectrometry 105 F i ... tiamulin (D) Simultaneous Determination of Various Macrolides by Liquid Chromatography /Mass Spectrometry 107 Table Summarized mass spectra of the drugs used experiments D ru gs Ery th ro my ... ochemical detection are very sensitive to environmental condition The determination method by gas chromatography -mass spectrometry (GC-MS) has been reported [5] This method needs the derivatization procedures...
- 6
- 391
- 0
Báo cáo khoa học: "Determination of Roxithromycin by Liquid Chromatography/ Mass Spectrometry after Multiple-Dose Oral Administration in Broilers" pdf
... estimated by the linear regression analysis of the log-transformed tissue concentrations, and was determined at the time when the Determination of Roxithromycin by Liquid Chromatography /Mass Spectrometry ... － － － － － － － － － － －, not detected or under LOQ Determination of Roxithromycin by Liquid Chromatography /Mass Spectrometry after Multiple-Dose Oral Administration in Broilers 39 Ta ble Concentration ... and Cecilia, A Confirmatory method for macrolide residues in bovine tissues by micro-liquid chromatography-tandem mass spectrometry J Chromatogr A 2001, 926(1), 97-104 Demotes-Mainaird, F., Vincon,...
- 5
- 263
- 0
Tài liệu Báo cáo khoa học: A functional polymorphism of apolipoprotein C1 detected by mass spectrometry docx
... Structural polymorphism of apolipoprotein C1 M S Wroblewski et al also suggest approaches that might be used to determine the role of N-terminal truncation of ApoC1 Results Profile analysis The MALDI- TOF ... disulfide-linked to cysteine (m ⁄ z ¼ 13881, Fig 1) Polymorphisms appear as a double peak for each form of a given protein The peaks differ by the mass change produced by the amino-acid substitution Figure ... peptide mass fingerprint from MALDI- TOF MS showed m ⁄ z values corresponding to all eight theoretical peptides plus the peptide of the truncated protein (residues 1–13, TPDVSSALDKLKE, theoretical mass...
- 9
- 529
- 0
TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx
... analysis of the organic solvent is the preferred method of analysis, either untreated or simply diluted in a suitable solvent Direct organic solvent analysis demands some ... routine analysis of such solvents, it is essential that the vapor pressure is controlled by cooling the spray chamber, where the sample aerosol is generated This can be best affected by means ... routine analysis of organic solvents, the normal peristaltic pump tubing is replaced with PTFE tubing connected directly to a nebulizer that draws the sample solution into the spray chamber by self-aspiration...
- 6
- 610
- 0
Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... detection and analysis of macromolecular complexes These protein complexes are detected at different mass- to-charge ratios (m ⁄ z), separated by differences in their time-of-flight inside the mass analyser ... orthogonal time-of-flight mass spectrometer (Micromass LC-T; Waters, Manchester, UK) modified for high mass operation and operating in positive ion mode [32] To monitor RNA cleavage by Kid protein a robotic ... 240–243 Heck AJ (2008) Native mass spectrometry: a bridge between interactomics and structural biology Nat Methods 5, 927–933 Sharon M & Robinson CV (2007) The role of mass spectrometry in structure...
- 14
- 477
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng 9 tr 25