... GFP control cells was not detected When the number of infused cells was raised to × 105 cells, both Sall4A and Sall4B transduced cells repopulated of recipients, whereas GFP transduced cells did ... by washing cells times, then G-CSF (R&D Systems, Minneapolis, MN) was added to cells at a final concentration of 200 ng/ml CFU assay of BM cells Tubes of MethoCult® GF M3434 (Stem Cell Technologies, ... Sall4B expanded cells, × 10 15 WT-like B6.SJL Sall4B expanded cells, × 106 11 WT-like B6.SJL GFP-infected cells, × 106 15 WT-like 11 WT-like B6.SJL Number of injected cells GFP-infected cells, × 10...
... study ofembryonicstemcells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cellsEmbryoniccells Fig Epigenetic distances between ES cells and other stemcells ... methylation profiles Epigenetic study ofembryonicstemcells Differences in DNA methylation profiles between ES and other stemcells Comparing ES cells with other stemcells established from developing ... contrast, TS cells derived from the trophectoderm (TE) of blastocysts contribute only to placenta Although there is an apparent ED between ES cells and EG cells, the ED of TS cells to ES cells (77)...
... from Murine EmbryonicStemCells 11 Development of Lymphoid Lineages EmbryonicStemCells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation ofEmbryonicStemCells 13 ... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from EmbryonicStemCells The In Vitro Differentiation of Mouse EmbryonicStemCells into Neutrophils 10 Development and Analysis of ... Mouse EmbryonicStemCells YIJING CHEN, JAY L VIVIAN, AND TERRY MAGNUSON 406 Section IV Differentiation of Monkey and Human EmbryonicStemCells 29 Growth and Differentiation Monkey ES Cellsof Cynomolgus...
... promotes self- renewalof mouse embryonicstemcells Dev Biol 1999, 210:30-43 Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonicstemcells Dev ... regulators of self- renewalof ES cells It is recently documented that ERK2 null ES cells fail to commit into neural and mesodermal cells [21-24] Similarly, rare brachyury (T) expressed cells were ... detect the Sox1-GFP+ cells from day to day (Fig 3A) The number of Sox1+ cells became 20% of total cells on day and reached the plateau, 50% of total cells, on day Treatment of FGF1 consistently...
... totipotential embryonicstemcells in tissue engineering of bone [17,18] Embryonicstemcells (ESCs) are routinely derived from the inner cell mass of blastocysts and represent pluripotential embryonic ... in mouse embryonicstemcells Cloning StemCells 2004, 6(4):386-391 Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D: Inhibition of pluripotential embryonicstemcell differentiation ... mineralization of osteogenic cells derived from human embryonicstemcells Tissue Eng 2004, 10(910):1518-1525 Evans MJ, Kaufman MH: Establishment in culture of pluripotential cells from mouse...
... Human embryonicstem cells: theoretical approaches Blastomere biopsy and culture Prior to the blastocyst stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which ... scoring system Stemcell research: social and political factors Public sharing of information about the basic science of ESCs has proven to be important, since those who are aware of the stemcell ... Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply ofembryonicstemcells without production of whole embryos or compromising extant...
... 1.1 Current paradigm of the cellcycle and cancer 25 1.1.1 The general schema ofcellcycle progression: simplifying complexity 25 1.1.2 The control ofcellcycle progression ... during the cellcycle in U2OS cells 157 Figure 25C TRIP-Br2 is differentially expressed during the cellcycle in COS-7 cells 158 Figure 26A-D Rapid turnover of C-terminal ... 235 Figure 40 CD8+ cytotoxic T cells and CD4+ helper T cellsof TRIP-Br2 wildtype and mutant mice (spleen) 236 Figure 41 Proportion of CD4+ memory T cellsof TRIP-Br2 wildtype and mutant...
... cells Put together, all these evidence suggest that the regulationof Lefty1 by OCT4, KLF4 and NANOG may be part of the ES cellselfrenewal mechanism The role of Lefty1 in regulationof ES cell ... seeding densities of 300 and 500 cells per 56cm2 (surface area of each well of a well dish is 56cm2) When 300 cells were reseeded, the number of secondary colonies formed for cells transfected ... compare the extent of differentiation between Lefty2 RNAi cells and control cells a) Two seeding densities (300 and 500 cells) were performed for one of the shRNAs while b) 300 cells were replated...
... embryonic development and stem cell- specific expression of Lefty has been observed in mouse and human, its functional significance in embryonicstemcells has never been demonstrated A part of ... specification of ES cells is a SMAD3 dependent process On the other hand, SMAD3 plays no role in trophectodermal 158 specification of ES cells as inhibition of SMAD3 activity does not lead to ES cell ... Nodal signaling in both the maintenance of ES cellselfrenewal and induction of ES cell differentiation have both previously been reported A key objective of this thesis is to reconcile the differences...
... ES cells and HEK293 Transfection of plasmids into mouse ES cells and HEK293 cells were performed using Lipofectamine2000 (Invitrogen) 167 4.5.2 Human ES cell transfection For transfection of ... 4.14.1 Enhancement of Nodal/Activin signaling E14, TAG1 or tet-Nodal E14tg2a cells were seeded in ES cell medium at a density of 676 cells/ cm2 without gelatin The next day, the seeded cells were subjected ... Generation of LEFTY2 conditioned medium HEK 293T/17 (ATCC: CRL-11268) cells were seeded in growth medium 24 hours before transfection at a density of 6.28 × 106 cells per 15-cm culture dish The cells...
... pluripotency of human embryonicstemcells in the absence of feeder layers StemCells 23, 489-495 Besser, D (2004) Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonicstemcells ... expression cloning of Nanog, a pluripotency sustaining factor in embryonicstemcellsCell 113, 643-655 Chambers, I., and Smith, A (2004) Self- renewalof teratocarcinoma and embryonicstemcells Oncogene ... signaling fails to maintain self- renewalof human embryonicstemcellsStemCells 22, 770-778 Darr, H., Mayshar, Y., and Benvenisty, N (2006) Overexpression of NANOG in human ES cells enables feeder-free...
... molecular mechanism underlying selfrenewal and cell fate determination of ES cells 1.2 Molecular basis underlying ES cellselfrenewal Despite the importance ofstemcellself renewal, we are only beginning ... ES cells for the study of pluripotency and pluripotent cellselfrenewal Scientists have recently reported the successful reprogramming of mouse and human somatic cells into pluripotent stem cells, ... with the regulationof the growth and selfrenewalof iPS cells rather than the induction of pluripotency per se, as overexpression of Klf4 and c-Myc resulted in formation of tumour cells that...
... target in mouse Es cells LeftyB (human Lefty1) is regulated by Wnt pathway High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed ... ES cells LeftyA (human Lefty2) is regulated by Wnt pathway Expression detected in ES cells and F9 murine teratocarcinoma Differential expression pattern between ES cells and differentiated cells ... Member of TGFsuperfamily Involved in Nodal signaling Nodal antagonist Criteria met • • • • High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells...
... Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonicstemcellsStemCells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human ... Marshall VS, Jones JM Embryonicstemcell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonicstemcell lines from human ... correlated to loss ofcell viability upon exposure to low temperature It was observed that some cells detached after exposure to low temperature Virtually all of the detached cells were determined...
... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... differentiation of mouse embryonicstemcells to hematopoietic cells on an OP9 stromal cell monolayer Methods Enzymol 365, 72–83 21 Strahl BD, Ohba R, Cook RG & Allis CD (1999) Methylation of histone ... fetal liver cells These results indicate acetylation plays a critical role in the transcriptional potentiation and developmental regulationof these genes in progenitor cells or cells that have...
... growing HeLa cells were incubated with nocodazole Cells detached from the bottle by shaking were collected and named mitotic (M) cells Residual cells were collected and named G2 cells These cells and ... transitions in the cellcycle are also required to prevent re-replication of DNA in a single cellcycle Inactivation of CDK1 leads to re-replication of DNA in eukaryotic cells including human cells [7] ... Linearity of DAPI-stained DNA content of a nucleus was preserved throughout such treatments; i.e the DNA content of G2, M cells are almost double that of G1 cells and the DNA content of S cells ranges...
... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... TRA-1(-) cells VEGF-R2(+) / VE-cadherin (-) / TRA-1 (-) cells expansion with VEGF expansion with PDGF - BB VE-cadherin (+) cells VE-cadherin (-) aSMA (+) cells hES -ECs hES -ECs+MCs aSMA (+) cells ... Figure cells differentiated from human of the vascular Schematic representation of preparationES cellstransplanted Schematic representation of preparation of the transplanted vascular cells differentiated...
... Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical clustering of ... hES cells and adult cells was also significant (P < 0.05) In particular, the expression of miR-519b was 8-fold greater in hES cells than in EB cells and it was not even detected in adult cells ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note,...
... in mouse -5 embryonicstemcells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed to H2O2 10 M for a variety of times (0-60 ... Effect of dihydrotestosterone on mouse embryonicstemcells exposed to H2O2-induced oxidative stress 255 10, 423-426 12 Brook FA, Gardner RL The origin and efficient derivation ofembryonicstemcells ... role of dihydrotestosterone in benign prostatic hyperplasia Urology 2003, 61 (Suppl 1), 2-7 16 Chambers I The molecular basis of pluripotency in mouse embryonicstemcells Cloning Stem Cells...