secretion cell number and levels of akt and erk in granulosa cells in vitro
Báo cáo hóa học: " Effects of pegylated G-CSF on immune cell number and function in patients with gynecological malignancies" doc
... peripheral sites into the circulation However, the relative distribution of CD4 + T cells, CD8 + T cells, CD19 + B cells and NK cells (defined as CD3 - CD16 + CD56 + cells) within the lymphocyte ... stimulating factor treatment of Crohn’s disease is associated with the appearance of circulating interleukin-10-producing T cells and increased lamina propria plasmacytoid dendritic cells Clin Exp ... (0.8 cells/ μl, range 0.25-2) Mobilization of DC subsets and Treg cells We next investigated whether pegfilgrastim induced changes in the frequency of circulating DC precursors Cells were initially...
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báo cáo hóa học:" Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep" ppt
... and Erk in granulosa cells in vitro Cells treated with FSH or IGF alone showed an increase (P < 0.0001) in the secretion of inhibin-A, activin-A, follistatin and oestradiol, and cell numbers ... indicate that inhibition of the Akt and Erk pathways inhibit the stimulatory actions of FSH and IGF on cultured bovine granulosa cells and LH on theca cells in vitro Furthermore, inhibition of ... role for the Akt and Erk pathways in mediating the actions of FSH and IGF on granulosa cells and LH on theca cells in vitro and their role in follicle growth and oestradiol secretion in vivo While...
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báo cáo khoa học: "Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin" pot
... two domains and a secretion signal The N-terminal expansin domain and the C-terminal expansin domain are homologous to the catalytic domain of glycoside hydrolase family 45 (GH45) proteins and a ... composition and structure of the cell wall that governs the cell expansion direction and rate As in land plants, the primary cell wall of M denticulata Bréb consists mainly of pectins [10,11], cellulose ... denticulata expansinresembling proteins As the involvement of expansins in cell growth of green algae had not been examined previously, we concentrated the experiments on this class of proteins The full...
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Báo cáo y học: " Aberrant splicing of the hRasGRP4 transcript and decreased levels of this signaling protein in the peripheral blood mononuclear cells in a subset of patients with rheumatoid arthritis" pdf
... protein levels This finding would open a new insight in the field of monocyte-lineage cell biology and of the diseases where this lineage cells play a prominent role In the latter part of the ... Identification of hRasGRP4 mRNA and protein isoforms in CD14+ myeloid cells Circulating in vivo-differentiated, unfractionated human PBMCs and PBMC-derived CD3+ T cells, CD14+ myeloid cells, and CD14-/CD3-/CD19- ... solution containing 3,3'-diaminobenzidine tetrahydrochloride (DAB) Nuclear staining was done with hematoxylin, and the resulting stained cells were examined by light microscopy Immunoblotting After...
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Báo cáo y học: " Higher levels of Zidovudine resistant HIV in the colon compared to blood and other gastrointestinal compartments in HIV infectio." pot
... patients and were involved in writing the paper KN and KC were involved in designing the methods for collecting, analyzing and interpreting data, and helped putting the data and parts of the manuscript ... were cloned into pCR2.1 TOPO linearized vector using the TA cloning kit (Invitrogen, Burlington, ON) Plasmids containing relevant inserts were purified from bacteria using a Plasmid Mini-Prep Kit ... Wegelin J, Prindiville T, Dandekar S: Viral Suppression and Immune Restoration in the Gastrointestinal Mucosa of Human Immunodeficiency Virus Type 1-Infected Patients Initiating Therapy during...
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Báo cáo y học: "Duration of red blood cell storage and outcomes in pediatric cardiac surgery: an association found for pump prime blood" doc
... acquisition and interpretation GI was involved in data acquisition and interpretation, and manuscript drafting SB and AB were involved in data acquisition AF was involved in data interpretation and manuscript ... removal of the cannulas, heparin was reversed using protamine sulfate at a 1:1 ratio During and after CPB, additional RBCs were administered as needed in order to maintain a hematocrit value within ... that blood lactate levels during the ICU stay are initially higher in pediatric patients receiving a bloodless priming solution than in those receiving a blood-containing priming solution [11]...
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Study of human nasal epithelial stem or progenitor cell growth and differentiation in an in vitro system
... basal cells derived from NP epithelium and healthy controls, study of the functional cells showed several distinctions: 1) the intensity and extensity of MUC5AC staining were higher in the cells ... survived and more than 90% of cells proved to have properties of epithelial stem or progenitor cells in a chemical-defined medium Meanwhile, these cells successfully differentiated into functional cells ... changes of NP in vitro In conclusion, we established an in vitro model for validating and comparing hNESPCs morphology and functional activity in the cells from NP and healthy controls, and demonstrated...
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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
... containing 10% heat-inactivated fetal bovine serum (Nuoding, China), and the HT-29 cells were grown in DMEM medium (GIBCO, USA) also containing 10% heat-inactivated fetal bovine serum All of the cells ... Biology, Chinese Academy of Sciences (Shanghai, China) The breast cancer Bcap-37 and colon cancer HT-29 cell lines were maintained in our lab A375 cells and Bcap-37 cells were grown in RPMI1640 ... concentrations of matrine and RGD-LCL After 24 h of 200 treatment, the cells were collected, washed with cold phosphate-buffered saline (PBS), and resuspended at × 106 cells /mL in Annexin-V binding buffer...
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Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx
... transferrin required for the binding of gastrin, and of the regions in gastrin required for the interaction with transferrin, is obviously essential to a full understanding of the interaction The independent ... Ggly–transferrin interaction, to define the domains ⁄ residues of Ggly involved in the interaction (using Ggly mutants), and, finally, to determine the regions of transferrin required for the interaction ... Mutation of the N-terminal or C-terminal iron-binding sites of transferrin does not prevent interaction with Ggly N-lobe and C-lobe transferrin mutants were used to investigate the effect of loss of...
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Báo cáo sinh học: " Vascular endothelial growth factor regulates melanoma cell adhesion and growth in the bone marrow" ppt
... were incubated for 30 min, in the case of B16M cells, or for 60 in the case of A375M cells, at 37°C before unattached cells were removed by washing three times with fresh medium The number of adhering ... antiEndotoxin BM Stromal Cells TNFalpha TNF Receptor-Expressing Melanoma Cells COX-2 BMSC and Melanoma Cell Secretion of VEGF BM Stromal Cell Expression of VCAM-1 VEGF Receptor-Expressing Melanoma Cells ... common site of occult trafficking, infiltration and growth of blood-borne cancer cells, and their metastases are a major cause of morbidity [2] Not surprisingly, circulating cancer cells infiltrate...
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báo cáo hóa học:" Fibrin and poly(lactic-co-glycolic acid) hybrid scaffold promotes early chondrogenesis of articular chondrocytes: an in vitro study" docx
... To minimize cells lost during in vitro seeding procedure, we used fibrin to immobilize cells http://www.josr-online.com/content/3/1/17 and to provide homogenous cells distribution in PLGA scaffolds ... of 1-, 2- and 3weeks Measurement of cell proliferation activity of in vitro constructs Cell proliferation activity and cells viability was measured using MTT assay at day 1, 3, 7, 14 and 21 in ... easy to handle and deliver the cells [37] Similar finding was reported in the previous assessment of osteogenic potential utilizing human periosteum-derived progenitor cells and fibrin gel immobilization...
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Báo cáo lâm nghiệp: "Polyamines and ethylene during of Prunus avium L. in vitro rooting" pps
... results outlined above suggest that polyamines may be involved in the rooting process, probably in the stages of active cell division In fact, increases in intracellular PUT and SPD levels preceded ... of medium) incorporation into ethylene Ten shoots were placed in small flasks equipped with a side-arm, into which filter paper soaked in KOH was I inserted, and a center well containing either ... rooting percentages after 12-15 d and was thus used in all subsequent experiments Endogenous polyamine content during the rooting phase was characterized by a peak in PUT levels on d 9-11 in all...
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Báo cáo y học: "Inhibition of macropinocytosis blocks antigen presentation of type II collagen in vitro and in vivo in HLA-DR1 transgenic mice" docx
... results in down-regulation of antigen presentation and inhibits activation of CII-specific T cells in in vitro antigen presentation assays Since T cells specific for the glycosylated and non-glycosylated ... D, McInnes IB, Liew FY, Brewer JM: A novel dendritic cell- induced model of erosive inflammatory arthritis: distinct roles for dendritic cells in T cell activation and induction of local inflammation ... stained and imaged at the same gain and offset levels as the positive cells and no fluorescence was observed Results Mechanisms of CII uptake in macrophages and DCs To study the mechanisms of...
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báo cáo khoa học: "Stability and assembly in vitro of bacteriophage PP7 virus-like particles" pptx
... means of introducing an inter-dimer covalent bond Duplicating the coat gene and joining the two copies together in a single reading frame fuses the C-terminus of one monomer to the N-terminus of ... decline of soluble protein What might explain this behavior? Observations of the increased stability of single-chain dimers of MS2 coat protein have already been reported The MS2 single-chain ... non-reducing conditions and fractions of soluble protein and capsids remaining were determined minutes In contrast, when heated at 67°C in the presence of DTT the rate of capsid disappearance...
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báo cáo khoa học: "Cationic nanoparticles for delivery of amphotericin B: preparation, characterization and activity in vitro" potx
... outstanding anti-infective properties [18] Both amphotericin B and miconazole self-assemble and solubilize at hydrophobic sites of DODAB or DHP bilayer fragments in water solution exhibiting in ... molecular weight was an increase in size, an increase in zeta-potential and a decrease of % of cell viability (Table 2) Table summarized the different properties of assemblies at low and high P One should ... or in Milli-Q water by centrifugation (3000 rpm/10 minutes), pelleting and resuspension The final fungal cell suspension was prepared by adjusting the inoculum to × 107 cfu/mL and then diluting...
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Báo cáo y học: "Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" pptx
... advantage in cells pretreated with interferon beta To study the effects of interferon on replication of both viruses, Vero cells were pre-treated with increasing concentrations of interferon beta in ... shown in Figure 4A, fragments of the expected sizes were obtained The linearized BAC cDNA and a PCR product containing the nucleocapsid gene were in- vitro transcribed and cotransfected in BHK cells ... C-terminus of ORF 7b As shown in Figure 6A, a protein band corresponding to the predicted molecular weight of the 7b protein (5.3 kDa) was specifically detected in rSCV7bflaginfected cells using...
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Báo cáo khoa học: " Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" docx
... advantage in cells pretreated with interferon beta To study the effects of interferon on replication of both viruses, Vero cells were pre-treated with increasing concentrations of interferon beta in ... shown in Figure 4A, fragments of the expected sizes were obtained The linearized BAC cDNA and a PCR product containing the nucleocapsid gene were in- vitro transcribed and cotransfected in BHK cells ... C-terminus of ORF 7b As shown in Figure 6A, a protein band corresponding to the predicted molecular weight of the 7b protein (5.3 kDa) was specifically detected in rSCV7bflaginfected cells using...
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Isolation and molecular characterization of genes associated with shoot regeneration of mustard (brassica juncea) in vitro
... increase in ethylene production of Chinese cabbage in vitro In tobacco suspension cells, the level of cellular PAs increased in response to exogenous application of ethylene and auxin (Park and ... resulted in increased incorporation of labelled methionine into spermidine (Biondi et al., 1990) Treatments of plant tissues with AVG and cobalt ions also resulted in an increase in the activities of ... non-differentialting cells In cell culture of ginseng, the addition of putrescine, spermidine, or spermine to the medium promoted the formation of somatic embryos up to 4-fold without affecting callus...
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Developing and evaluating in vitro effect of pegylated liposomal doxorubicin on human cancer cells
... lung non-small cancer cell line (A549 cells) and HT 29 colon cancer cell line was determined by using the MTTdye reduction assay Briefly, 1.0 × 104 cells/ well was seeded in 96-well flat-bottom ... purchased from company ATCC, USA .Cells were grown in Eagle's minimum essential medium (ATCC, USA)containing 10 % Fetal Bovine Serum and 1%streptomycin-penicillin Instruments: The evaporation system ... doxorubicin begin to exert cytotoxic effects and the effect is strongest after 72 hours Recently, Cheng et al also reported that IC50 of PEG-liposome containing doxorubicin in- vitro on cell line A549...
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