... its native structure after the insertion of foreign sequences and in the presence and absence of Ca2+ ions, so that the influence fromthe host protein to the inserted sites is minimized [14 ] Our ... and a is the radius ofthe molecules The diffusion constant of a dimer is ideally expected to be approximately 79% ofthe value of a monomer [ 21] The diffusion constants of engineered protein CD2.STIM1.EF ... spectra shown in Fig 2A clearly demonstrate that the conformation of CD2 is unchanged After the insertion ofthe helix–loop–helix EF-hand domain from STIM1, the helical content ofthe engineered...
... shift ofthe rate constants ofthe branched part of mechanism, i .e increased cleavage at the expense of complex formation Therefore, the products ofthe reaction were analyzed by SDS/PAGE (Fig ... set of rate constants for all TM concentrations The lower panel shows the residuals ofthe fit expressed as the difference between the experimental and calculated values (B) Shows the calculated ... exclusive, both in the presence and absence of VN Furthermore, the data presented here is in agreement with a mechanism in which the rate of thrombin inhibition by PAI -1 is dependent onthe rate of...
... must be tempered by the enormous gulf between identifying genotypic risk and successful development of therapeutic interventions The even thornier issues ofthe socioeconomic ramifications of genotype ... genotype profiling need legislative consideration now if the worst elements of eugenic prejudice are to be avoided Steven Opal (Pawtucket, RI, USA) gave an overview ofthe complex integration between ... issues of terrorist attacks and mass casualty response The lessons learned from recent and ongoing world events are that education, preparedness and practice [36] are the vital elements in meeting...
... beyond merely the enactment of legislation While "discrimination" is not defined in the Covenant, its meaning may be ascertained by reference to the usage developed in the references and interpretation ... only once a year for a three-week session At this session, they generally review the reports of six countries Members, who are elected by the Economic and Social Council, not receive remuneration ... available; (2) does the allocation of health expenditures in national and regional budgets represent a fair balance between women's health needs and other concerns; and (3) are illnesses and other health...
... are apparently already in development The debate regarding the toxicity of oxygen andthe formation of free radicals continues despite the absence of demonstrated effectiveness of scavenging therapies, ... Society, the European Respiratory Society, the European Society of Intensive Care Medicine, andthe Societe de Reanimation de Langue Francaise, and approved by the ATS Board of Directors, December ... multicentre study are expected to be presented at the 22nd International Symposium on Intensive Care and Emergency Medicine, in Brussels in 2002 reports The round-table conference preceding this year’s...
... product-destination level would lead to overestimate thee ect ofthe credit shock onthe drop in exports during the 2008 crisis by 95% We compute thee ect of credit onthe size and frequency ofthe firm’s export ... destinations Then, estimations based on comparing the outcomes of firms related to affected and non affected banks confound thee ect ofthe lending channel with the heterogeneous impact ofthe ... altering, both, the size and frequency of shipments The elasticities ofthe frequency and size of shipments to credit are 0 .14 and 0 .12 , respectively Onthe extensive margin, credit supply affects...
... arrangement, expression vectors were constructed that separately encoded the N-terminal half (residues 1 15 3) andthe C-terminal half (residues 15 1–299) of At3g16450 .1, and these were used to prepare ... severe line broadening, probably arising from internal dynamics of these residues onthe intermediate time scale for chemical shifts As a result, these are the least well-defined regions ofthe ... [25] Due to the lack of NOEs, the relative orientation between the N- and C-terminal domains could not be defined (B) Primary sequence of At3g16450 .1 The sequences that correspond to the N-terminal...
... the similarity ofthe aA–aE region andthe region upstream of a4 was relatively low, the N-terminal region (aN–a1), the aF–aG region andthe C-terminal region (a4–a5) were well conserved between ... been experimentally evidenced C elegans is the best genetic model The generation of transgenic C elegans has been established and is easier than that of other animal models [35] The results of ... GOA -1 and Gao [ 31, 32] The five regions ofthe Ga-subunit involved in receptor recognition are the a2 helix, the b6 ⁄ a5 loop, the a5 helix andthe N- and C- extreme termini [ 31] In addition, the...
... nine-stranded antiparallel b-sheet in the center ofthe domain with the active site ofthe enzyme on one side andthe Rieske center onthe other side ofthe sheet (Fig 3) Covering one side ofthe ... ˚ extends 20 A fromthe center ofthe funnel, they form the base ofthe b subunit (Fig 3) The last four residues in the C-terminal coil (residues 17 1 17 4) are deeply anchored inside the core of ... favorable region ofthe Ramachandran plot; all ofthe 11 outliers were located on b-turns in the a subunits and present well-defined electron density except for Leua238 Like other members ofthe naphthalene...
... the Brazil nut 2S albumin One of these epitopes matched with the signal and propeptide regions, and therefore would not be present in the mature protein, but the remainder corresponded to the ... related allergen families, the elucidation ofthe 3D structure could help to gain a better understanding of their intrinsic allergenic properties Digestions were performed in either the presence ... problems were not encountered for peptide HPLC-ESI-MS of duodenal digestion (phase 2), which showed the presence of one new (penta)peptide derived fromthe small subunit, whilst 10 new peptides were...
... Table presents the results obtained in competitions between one ofthe early B1 clones (B1 .1; B1.2; B1.3) and one ofthe late B2 clones (B2.3 and B2.5) The B2 clones outcompeted the B1 clone in ... difference between the LTRs fromthe B1 and B2 strain from patient L in the human embryonic kidney cell line used, despite the occurrence of insertions in the B1 LTR However, the LTR fromthe primary ... indicated Transcriptional activity ofthe luciferase gene was tested in the presence of increasing concentrations of Tat The value is the average of three independent measurements; standard deviations...
... ofthe cone, and by the surface ofthe sphere inside the cone, a “solid sector.” /6/ And when two cones having the same base have the vertices on each ofthe sides ofthe plane ofthe base, so ... distinguishes this clause, onthe relation between the volumes, fromthe next one, onthe relation between the surfaces In other words, the cylinder “itself ” is what we call the volume ofthe cylinder.” ... same tradition where we see the evidence for the presence of Isidore of Miletus, also has evidence for the presence of Leo the geometer,9 a leading Byzantine intellectual ofthe ninth century It...
... focused on her while his guard studied the barn, trying to determine why the lingering scent of his own kind would be there Her long red hair and fierce green eyes gave away her Irish heritage She ... and skirted around the two men as she went to the stalls “Can you ride a horse?” Both of them glanced at her nervously Her scent blew by them with the breeze and they fought to control their natural ... at each other After only a few minutes, he saw the large herd of Angus cattle they were heading towards Emily was a few yards ahead of him so he studied her again In the heat, she pulled her...
... case, subject and main verb are together at the beginning ofthe sentence, but in other case, they are separated or the writer delays the appearance ofthe verb Thus, one ofthe reason people ... Structure ofthe articles Each ofthe three main part of an article (the headline, the lead andthe body) has its own distinctive style and content 16 4.2 .1. 1The headline The headline is the extra ... proverb with changes Besides keeping all the basic elements ofthe proverb, the writer adds some new elements to the structure so as to emphasize the theme ofthe article or the opinion that he...
... before the tests and then the test is written onthe basis ofthe specifications After the test is written, the specification should be consulted again to see whether the test matches the objectives ... scores that a student gets on these tests are the main indicators of his or her performance during the whole semester There are different comments onthe results of these tests, especially the test ... developed a set of questionnaire to evaluate the test reliability from students perspective If the attitude and perception ofthe teachers onthe test had been studied, the results would have been...
... commented onthe role ofthe executive and their power in relation to the government control ofthe executive “Well they [the executives] neither have the given authority nor management skills They ... their increased knowledge and understanding ofthe change, they will then be able to impart their knowledge to other employees affected by the change Specifically, the rationale that underlies ... providers, professionals, and patients These include changes to the way healthcare is delivered through the emergence of new medical professions (e. g., genetic specialists), the devolution of minor...
... establishing the pathways at the level of intermediates and enzymes that catalyze their formation The subsequent step is the selection of targets for further studies at the level ofthe genes This knowledge ... greater detail points of interest, both theoretical and practical Consequently, the development of an information base on a cellular and molecular level has been considered as a cornerstone of ... a consequence of these events, the regulatory requirements and safety assessment studies are far greater, not only in the United States but also worldwide An improvement in the quality or the...