ribosome display for rapid protein evolution by consecutive rounds of mutation and selection
Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx
... description of the elementary steps of enzyme–target binding and catalysis and then uses the rapidequilibrium approximation for protein protein interactions for a systematic simplification of the model ... order and the degree of processivity are related, and, if so, whether this relationship contributes to the high specificity and timing of signaling processes Regulation of protein conformation by ... account for both enzyme–target binding and the catalysis K and P denote the free concentrations of kinase and phosphatase, respectively, and Ln and Qn are the respective dissociation constants for...
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Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot
... I., Merlevede, W & Goris, J (1995) Molecular cloning and developmental regulation of expression of two isoforms of the catalytic subunit of protein phosphatase 2A from Xenopus laevis Biochem Biophys ... Goris, J., Hofsteenge, J., Merlevede, W & Hemmings, B.A (1993) Structure and expression of a 72 kDa regulatory subunit of protein phosphatase 2A Evidence for different size forms produced by alternative ... and Xenopus lysates, which recognises the PR65 a and b isoforms equally well The best anti-C monoclonal was found to be F2 6A10, and it recognizes PP2AC in rabbit, mouse and Xenopus oocytes and...
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Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx
... expression levels of both SC and IgA (A) and the Mr of SC (80 kDa) and IgA (160 kDa) complexing of IgA and J-chain is a prerequisite for SC binding Therefore, the correlation between the IgA and SC levels ... Detection of IgA, pIgA, and SIgA expression and bound SC Transfected and fused CHO-K1 cells were analysed for production of IgA, pIgA or SIgA and IgA bound SC by ELISA on supernatant of outgrown ... cell IgA-29, the pIgA clone pIgAD, and eight SIgA clones (SIgA-1–8) were analysed by ELISA for expression levels of IgA and total SC (Fig 2A) and by dot blot for total SC (Fig 2A) The IgA expression...
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Báo cáo khoa học: Inhibition kinetics of catabolic dehydrogenases by elevated moieties of ATP and ADP – implication for a new regulation mechanism in Lactococcus lactis potx
... isozymes of each of these dehydrogenases, e.g L lactis IL1403 contains three genes for LDH, two for ADH and two for GAPDH [20], which all could have been affected one way or another by both ATP and ... condence intervals and rmse and lower adjusted R2 (0.950 and 0.968 for ATP and ADP, respectively) were obtained than with Eqn (3) (Table 1) Table The estimated VMAX and KM (mM) of the cofactor substrate ... pool of ATP and ADP [using Eqn (6) and parameter values in Table 2], the 1846 A Fig Multiple inhibition of LDH, ADH and GAPDH by ATP and ADP using YonetaniTheorell plots (A) Multiple inhibition of...
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báo cáo sinh học:" Increasing leadership capacity for HIV/AIDS programmes by strengthening public health epidemiology and management training in Zimbabwe" potx
... facilitated expansion of HIV programmes by supporting distance-based technical support for HIV prevention and treatment programmes and sharing of files and reports for monitoring and evaluation Discussion ... Training Officer, AIDS and TB Unit; and ANC Surveillance Officer MPH graduates in HIV/AIDS positions with other organizations include WHO HIV/AIDS Officer for Zimbabwe; the lead programme officer for ... number of persons trained for leadership and management of new HIV intervention programmes was insufficient Inadequate remuneration for public health officials and faculty was leading to loss of...
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Expressing gratitude by native speakers of english and vietnamese learners of english
... the use of thanking and expressing appreciation Expressions of gratitude by ES appear in the formula of Thanking or Thanking + Expressing appreciation And the act for expressing gratitude by VL ... available and secondly on the analysis of the Theory of Relevance and Co-operative Principle to identify the illocutions of the utterances Although expressing gratitude is performed by the use of several ... often used by VL than by ES On the contrary, expressing appreciation and offering reward are both preferred by ES Expressing gratitude by ES usually appears in the form of Thanking + Complimenting...
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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... concentrations Sequence analysis and modelling of LlChi18A and LlCBP33A Cloning and purication of LlChi18A and LlCBP33A The gene fragments coding for the mature proteins of LlChi18A and LlCBP33A were successfully ... containing 100 lm of (GlcNAc)14 was analysed at the start, in the middle and at the end of each series of samples The resulting average values of the standards (displaying standard deviations of < 5%) ... by a factor of 10 (see Materials and methods) (D) Chromatogram of (GlcNAc)6 degradation products generated by LlChi18 after of incubation with nM of enzyme The double peaks represent the a- and...
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Tài liệu Report produced for the EC funded project - INNOREGIO: dissemination of innovation and knowledge management techniques pdf
... companies and crisis management We communicate state of the art of management know-how in presentations, seminars, programs and events We are a platform for the exchange of information and the ... largest discrete block of time and resources handled by most PM software Rescheduling Rescheduling is a feature of most PM software that recalculates the start and finish dates of all uncompleted ... development of goods and services Project Management is a set of principles, methods and techniques for effective planning of objective-oriented work, thereby establishing a sound basis for effective...
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PLANNING THE PROJECT MANAGEMENT WAY: EFFICIENT PLANNING BY EFFECTIVE INTEGRATION OF CAUSAL AND RESOURCE REASONING IN REALPLAN pdf
... SHUF4-GP-TOT SHUF6-GP-TOT SHUF8-GP-TOT SHUF10-GP-TOT 1000.0 with varying number of blocks and robots Performance of Graphplan in Shuffle Problem Đ ÞɨÙÍTr'IÈ ÊỊÇ Ê I §ÇĨF¨ÊͤÙɨʼÍ&'IÉâB¼Ú¾Ëpx¨È¾Í©É@§ÍS§Ýâ§ỊÉâ§Ỵ©©©¤Ỵ1¾ÍØϨÊÍټɨʼÍÌ ... CHOICE OF TECHNIQUES FOR SOLVING RESOURCE SCHEDULING PROBLEM C O N V E N T I O N A L P L A N N E R DECLARATIVE PROCEDURAL SAT (BLACKBOX) GRAPHPLAN GP-CSP SAT MANY CHOICE OF TECHNIQUES FOR SOLVING ... SHUF8-PS-TOT SHUF10-PS-TOT 100.0 1000.0 in the Shuffle problem with varying number of blocks and robots Performance of Graphplan v/s Planning+Scheduling ¸ ¸ fh h us os
s
ms om m X...
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báo cáo hóa học:" Research Article Entire Solutions for a Quasilinear Problem in the Presence of Sublinear and Super-Linear Terms" potx
... proof of Theorem 1.1 Appendix Proof of Lemma 2.4 The proof of item iv is an immediate consequence of Lemma 2.3 i The item v follows by Lemma 2.3 i and ii using Lebesgue’s Theorem Proof of (i) and ... the proof of Theorem 1.6 10 Boundary Value Problems Proof of Main Result: Theorem 1.1 To complete the proof of Theorem 1.1, we will first obtain a classical and positive lower solution for problem ... the proof of Theorem 1.3 Proof of Theorem 1.5 To prove Theorem 1.5 , we will first show the existence of a solution, say uk ∈ C2 B 0, R \ {0} ∩ C1 B 0, R ∩ C B 0, R , for each k 1, 2, , for the...
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Báo cáo hóa học: "Research Article The Problem of Scattering by a Mixture of Cracks and Obstacles" pptx
... R2 , and H0 is a Hankel function of the first kind of order zero By making use of the known jump relationships of the single- and double-layer potentials across the boundary ∂Ω see 5, 11 and approaching ... solution of 2.5 and 2.3 , then u ∈ H 1/2 Γ and ∂u/∂ν ∈ H −1/2 Γ The proof of this lemma can be found in 11 We are now ready to prove the existence of a solution to the above scattering problem by ... 10871080, Laboratory of Nonlinear Analysis of CCNU, COCDM of CCNU References R Kress, “Frechet differentiability of the far field operator for scattering from a crack,” Journal of Inverse and Ill-Posed...
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Research for a Future in Space: The Role of Life and Physical Sciences ppt
... processes as the formation and movement of ocean waves and molecular signaling in bacteria The pervasive force of gravity has had profound and myriad effects on the evolution and development of terrestrial ... understanding of the effects of reduced gravity on the many and complex systems of the human body The skeletal system of animals provides a solid framework for structural support, protection, and ... absence of gravity need to be developed for use in life support, thermal control, power production and liquid fuels storage and handling 17 Research for a Future in Space: The Role of Life and Physical...
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Báo cáo y học: " HIV-1 resistance conferred by siRNA cosuppression of CXCR4 and CCR5 coreceptors by a bispecific lentiviral vector" ppt
... strategies offers a promising alternative approach for controlling and managing HIV disease A number of previous approaches that involved the use of transdominant proteins [1-3], decoys [3-7], and ribozymes ... cells and macrophages which are the main targets for the virus Our results showed for the first time that expression of both CXCR4 and CCR5 siRNAs in combination is possible by the use of lentiviral ... consisting of cPPT and CTS (to facilitate the nuclear import of the viral preintegration complex) and the WPRE (to promote nuclear export of transcripts and/ or increase the efficiency of polyadenylation...
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Báo cáo y học: "Relationship between psychosomatic complaints and circadian rhythm irregularity assessed by salivary levels of melatonin and growth hormone" ppsx
... Children study of the WHO [15] and psychosomatic complaints scale for adolescents confirmed by factor analysis at assessment of validity and reliability of the scale [16] The questionnaire for this ... determined by analyzing melatonin and GH as a pair Therefore, we suggest that the asynchronicity and lack of peak secretion for the two hormones reflect an irregular circadian rhythm Chronotypes and ... isolation in adults [14] The purpose of this research was to explore the lifestyle of today’s youth by utilizing the characteristics of melatonin and GH, both of which show circadian rhythms under...
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Báo cáo y học: "Duodenal enteroglucagonoma revealed by differential comparison of serum and tissue glucagon reactivity with Siemens'''' Double Glucagon Antibody and DakoCytomation''''s Polyclonal Rabbit Anti-Human Glucagon: a case report" ppsx
... of Author Details 1Department of Surgery, Division of Transplant Surgery and Hepatobiliary Surgery, Henry Ford Hospital, West Grand Boulevard (CFP-2), Detroit, MI 48202, USA and 2Department of ... assembled the patient information, formulated in depth analyses of the known literature, performed the operative interventions and managed the recovery of the patient, constructed and edited the manuscript, ... referred from an outside hospital for evaluation and treatment of obstructive jaundice secondary to a periampullary mass Patient informed consent for publication of findings was obtained during...
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Báo cáo y học: " Rates and risks for prolonged grief disorder in a sample of orphaned and widowed genocide survivors" potx
... approved by the University of Konstanz Ethical Review Board and by Rwanda’s National Institute of Statistics, Kigali Eligible subjects were widows (female gender) and orphans (female and male ... the design and the coordination of the study TE participated in the design of the study and contributed to the interpretation of findings and writing of the paper All authors read and approved ... reactions and the comparison of findings across studies have been limited by the absence of universally accepted, standardized criteria for diagnosis Investigators use different criteria for grief...
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Báo cáo y học: "Inhibitory effects on HAV IRES-mediated translation and replication by a combination of amantadine and interferon-alpha" doc
... frame of firefly luciferase (Fluc) flanked by the first four amino acids of HAV polyprotein and by 12 C-terminal amino acids of VP1 This segment is followed by P2 and P3 domains of HAV polyprotein ... LY, Tatsuo Kanda, FI and OY conceived and designed the study LY, Tomoko Kiyohara and Tatsuo Kanda performed the experiments LY, Tomoko Kiyohara, Tatsuo Kanda and FI analyzed data and wrote the ... the replication of many DNA and RNA viruses and is also used as a drug for the treatment of Parkinson’s disease [2] It is known that the M2 protein of influenza A virus is a target of amantadine...
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Báo cáo y học: " Human Immunodeficiency Virus Type 1 Nef protein modulates the lipid composition of virions and host cell membrane microdomains" pot
... sequences of all nef inserts of these proviral clones were verified by sequencing of both DNA strands The expression plasmids for wt CD4 and the 2Cm and 4C CD4 mutants were kindly provided by Dr Jin and ... implicated as platforms for central cellular processes such as signal transduction and protein trafficking but are also utilized as preferred sites for entry and egress of a number of viruses, including ... disruption of DRM association of Nef correlates with a loss of infectivity enhancement [19] However, DRM recruitment of HIV structural proteins was not observed in another study and DRM enrichment of...
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Protein turnover in tissues effects of food and hormones
... image features Non-negative matrix factorizations Các bước băm ảnh số Feature Extraction Randomization Quantization Encoding 10 Các bước băm ảnh số Feature Extraction Lấy nét đặc ... cho băm ảnh số Đây bước có nhiều báo, nghiên cứu đề xuất phương pháp để lấy nét đặc trừng ảnh Randomization Trong bước liệu vector lấy từ bước Freature Extraction tạo băm việc sử dụng khóa...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose