region of nf90ctv is involved in interaction with rev
Báo cáo y học: " Tenofovir treatment augments anti-viral immunity against drug-resistant SIV challenge in chronically infected rhesus macaques" pot
... deviation of 5–7 time points within 2.5 years before administration of tenofovir d Mean ± standard deviation of days -14, -6 and before administration of tenofovir e Day before administration of tenofovir ... live, attenuated SIV, mirroring in certain aspects the clinical progression of chronic HIV-1 infection in humans, including increasing viral burden and progressive loss of CD4+ T cells The immunologic ... when inoculated intravenously at a dose of 104 TCID50/ml, is able to infect and replicate in the majority of rhesus macaques receiving concurrent antiretroviral treatment with tenofovir Administration...
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Báo cáo y học: "High-Resolution Flow Cytometry: a Suitable Tool for Monitoring Aneuploid Prostate Cancer Cells after TMZ and TMZ-BioShuttle Treatment"
... Hoeijmakers JH Genome maintenance mechanisms for preventing cancer Nature 2001; 411: 366-374 Jurgensmeier JM and Bauer G Interference of bcl-2 with intercellular control of carcinogenesis Int J Cancer 1997; ... and Levine JS Necrosis and apoptosis in acute renal failure Semin Nephrol 1998; 18: 505-518 57 Tisdale MJ, Antitumor imidazotetrazines XV Role of guanine O6 alkylation in the mechanism of cytotoxicity ... Denkert C Expression of the ELAV-like protein HuR in human prostate carcinoma is an indicator of disease relapse and linked to COX-2 expression Int J Oncol 2008; 32: 341-347 Meisner NC, Hackermuller...
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báo cáo hóa học:" Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor antagonist" potx
... Representative histogram of AF488-MCP-1 staining in human whole blood Representative histogram of AF488-MCP-1 staining in human whole blood Cells were stained with AF488-MCP-1 in the presence ... effect of overnight shipping on inhibition of internalization of AF488-MCP-1 by the receptor antagonist a direct comparison of the effect of overnight storage on the IC50 of the CCR2 inhibitor ... to demonstrate inhibition of ligand internalization at low concentrations of CCR2 inhibitor, was determined by serially diluting the CCR2 antagonist into whole blood and incubating for hour at...
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báo cáo hóa học:" Static platelet adhesion, flow cytometry and serum TXB2 levels for monitoring platelet inhibiting treatment with ASA and clopidogrel in coronary artery disease: a randomised cross-over study" potx
... measurements of fibrinogen-binding or expression of P-selectin Presented results are the mean-% of fibrinogen-binding and Pselectin expression ± SEM Reference values (obtained earlier during routine analysis ... measurements but instead compared the individual variables After in vitro activation, binding of fibrinogen and expression of P-selectin were (with the exception of ADP-induced P-selectin expression ... Platelet expression of P-selectin and binding of fibrinogen were measured by flow cytometry as indicators of platelet activation [29-32] To tubes intended for fibrinogen binding analysis, 10 μL FITC-conjugated...
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Báo cáo khoa học: " Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules" ppsx
... Comparison of labeling with CADO34A to labeling with BAQ44A revealed the pattern of labeling is similar to the labeling pattern obtained with BAQ44A for T and B lymphocytes Simultaneous labeling with ... one of the mAbs indicates the epitopes detected are sterically close on the same molecule, with the binding of one mAb interfering with the binding of the second mAb These methods of analysis ... Ken (diagonal co-labeling) CD5 (inferred from pattern of FC labeling) CD8 (diagonal co-labeling with ISC27A, ISC29A, ISC38A) CD8 (diagonal co-labeling with1 2.C7, ISC29A, ISC38A, RT1A) CD8 CD8...
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Báo cáo y học: "Flow cytometry analysis of glucocorticoid receptor expression and binding in steroid-sensitive and steroid-resistant patients with systemic lupus erythematosus" pps
... binding activity SPSS version 15.0 (SPSS Inc, Chicago, IL, USA) was used for the analysis Results Evaluation of FCM analysis of GR In several tissues and cultured cells lines, administration of ... difference in the expression and binding of GRs in SS patients compared with those in controls except for the binding of GR in T lymphocytes Similar results were also found in patients with nephrotic ... performed most of the statistical analyses and revised the manuscript XZ participated in study design, interpretation of data and revision of the manuscript WZ contributed in FCM analysis of GR WG...
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Báo cáo y học: "The basophil activation test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives" pot
... agonists of this receptor As previously observed in eosinophils [39], the interaction of prostaglandin D2 with the DP1 receptor limited the activation of basophils by this prostaglandin This suggested ... crucial in determining the magnitude of basophil responses during allergic processes since prostaglandin D2 is known to be involved in allergic diseases and asthma Using a similar approach, Heinemann ... the basis of side scatter characteristics (SSC, y-axis) and CD203c (x-axis) Left histogram depicts resting cells, basophils express low levels of CD203c (some of them are not distinguishable...
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Báo cáo y học: "Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts" pps
... event is triggered (Figure 4a) This kind of binary response is typical for apoptosis One key player of the apoptotic pathway is the enzyme caspase-3, which is activated at the onset of apoptosis in ... of the data distribution are visible in the simple scatter plot (b) The contour plot reveals the bimodality of the data (c) Coloring of points according to point density and (d) density map with ... and after removal of contaminants We observe a reduction in the proportion of data points with very small fluorescence values in both channels after removing contaminants This is reasonable because...
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Intake manifold design effect on air fuel mixing and flow for an LPG heavy duty engine
... received his MS Degree in 2008 in mechanic and energy engineering from the National School of Engineers of Sfax He is pursuing for his PhD Degree in energy engineering and engine researches of ENIS, ... degree in chemical engineering in 1981, his Doctor-engineer in 1984 and his PhD in chemical engineering in 1988 He is interested in Computational Fluid Dynamics CFD and energy He is author of different ... [7] is interested in the description of a linear acoustic model that has proven useful in obtaining a better understanding of the nature of acoustic wave dynamics in the intake system of an internal...
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Clinical Flow Cytometry – Emerging Applications Edited by Ingrid Schmid ppt
... 2004) This may also explain the atypical inflammation observed in invasive amoebiasis, in which there is decreased chemotaxis and disequilibrium in cytokine production This is supported by in vivo ... physical injury This response induces the infiltration of leukocytes and plasma molecules into regions of infection or injury Its main effects include increased blood flow to the region, increased vascular ... the Y-axis shows staining for phycoerythrin (PE) a) Autofluorescence; b) Isotype control, staining with mouse IgG1-FITC; c) Staining for subpopulations of CD3 coupled to FITC; d) Staining for...
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flow cytometry protocols 2d ed - teresa s. hawley
... of flow cytometry is the possibility of “gating.” Gating is the term used for the designation of cells of interest within a data file for further analysis It permits the analysis of subsets of ... been used with samples containing small numbers of cells DiOC2(3)/TO-PRO-3 staining: This method has been used in instruments with 488 nm and red illuminating beams separated in space; it is not ... more complete discussion of this aspect of immunophenotyping can be found in Chapter of ref 3.2.2 Gating the Data Gating is an analysis process of evaluating a restricted part of the file, for...
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flow cytometry protocols
... expression of the antigen of interest Positive controls are essentialfor establishing that the antibody used is capable of ident@ing the antigen of interest This type of sample is typically prepared with ... is most likely the result of nonspecific binding A useful method for determining the origin of secondary peaks in this type of control sample is to examine two types of plots These are FSC vs ... reagents Data analysis is a paramount issue in flow cytometry Analysis includes interpreting as well as presenting data that has been stored in list-mode files Data analysis is very graphically...
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hughes t. groove and flow - six analytical essays on the music of stevie wonder (диссертация)
... affirming the tonic, usually appearing in the song’s interior, with lyrics that remain constant with each hearing If there is no refrain, the chorus is the container of the song’s title Refrain: ... because I found some very interesting results in my initial investigation of it Chapter is an analysis of “Living for the City,” Wonder’s moving, epic marriage of soul, American history, the twelve-bar ... music is the question of the nature of the work itself My decision is in no way meant as an assertion of the primacy of album versions However, in the case of “Living for the City” the single is...
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Báo cáo hóa học: "Exhaustive expansion: A novel technique for analyzing complex data generated by higherorder polychromatic flow cytometry experiments" pot
... reasoning This distinction is clearly applicable to the interpretation of higher-order multiparameter flow cytometry data Herein, we apply a powerful inductive data analysis approach to two distinctly ... Each of the panels consists of mAbs The differences from the main panel are indicated both in the name of the panel and by the antibody listed in parentheses combinations of these markers to delineate ... memory T cells generated using WinList’s FCOM function, after setting positive and negative staining regions for each marker with FMO controls This resulted in delineation of 6,561 (3 ) sets per...
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CLINICAL FLOW CYTOMETRY – EMERGING APPLICATIONS pot
... 2004) This may also explain the atypical inflammation observed in invasive amoebiasis, in which there is decreased chemotaxis and disequilibrium in cytokine production This is supported by in vivo ... physical injury This response induces the infiltration of leukocytes and plasma molecules into regions of infection or injury Its main effects include increased blood flow to the region, increased vascular ... the Y-axis shows staining for phycoerythrin (PE) a) Autofluorescence; b) Isotype control, staining with mouse IgG1-FITC; c) Staining for subpopulations of CD3 coupled to FITC; d) Staining for...
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Báo cáo y học: " Diagnostic value of anti-human citrullinated fibrinogen ELISA and comparison with four other anti-citrullinated protein assays" potx
... resulting in an assay with 52% sensitivity at a specificity of 95% in a cohort of patients with established disease [9,10] An ELISA using a cyclic citrullinated peptide (CCP) derived from filaggrin ... reported in established RA Recently, the presence of citrullinated fibrin in the synovial membrane of RA patients [17] and the use of citrullinated fibrinogen to assay the serum antibodies to deiminated ... for ACPA determination within the context of a diagnostic investigation Patients were diagnosed by a clinician by reviewing of files and the patients were classified in accordance with the ACR...
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Báo cáo khoa học: "Cutaneous vascular reactivity and flow motion response to vasopressin in advanced vasodilatory shock and severe postoperative multiple organ dysfunction syndrome" pot
... combined infusion of AVP and norepinephrine did not increase oscillation frequency of the Doppler signal when compared with norepinephrine infusion alone At least in these patients, this finding ... microcirculatory response to a combined infusion of AVP and norepinephrine when compared with infusion of norepinephrine alone, using laser Doppler flowmetry in patients with advanced vasodilatory shock ... during supplementary AVP infusion by Dubois and colleagues [24] Interestingly, in this patient administration of AVP actually appeared to improve microcirculatory flow because of an increase in...
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Báo cáo y học: "Differential response of human basophil activation markers: a multi-parameter flow cytometry approach" pot
... upon intracellular staining of CD63 Therefore, our data suggest the existence of a main subpopulation of fully viable and responsive, but non degranulating basophils in human blood, and this is in ... did Discussion In this work human basophil activation was investigated in vitro by using a two laser multiparameter flow cytometry, a technique that is becoming prevalent in immunophenotyping ... basophil isolation and pooling has the advantages of washing out the plasma and the majority of platelets and of reducing the impact of the great variability of individual responsiveness [33] This...
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Influence of pipe length and flow rate on nano particle deposition in laminar circular pipe flows
... his B.S in Mechanical Engineering from the Tehran University in 1965 He received his M.S degree in Civil Engineering from the Purdue University 1968 and his Ph.D degree in Mechanical Engineering ... received his PhD from Queensland University of Technology in 2014 He is currently a lecturer in the department of Petroleum and gas Engineering, University of Salford, United Kingdom His main research ... be explained by the increasing inertia of larger particles since the inertia effect cannot be considered in the Eulerian method or mass diffusion equation and this is another advantage of the...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose