... includingcell-fate specification, regulation of differentiation, and control of cell proliferation and movement [45].Recently, we have shown that activation of the heat-shock response in the ciliate T. ... hypothesis that c-T1 and c-T2 have evolved sequence-based structural alterations that facilitate template nucleation of microtu-bules by the c-tubulin ring complex at cold temperatures.AbbreviationsqPCR, ... 45cycles of denaturation at 95 °C for 30 s and annealing and extension at 60 °C for 15 s each. Following amplification,melting curve analysis of the DNA was performed at tem-peratures in the...
... reaction) and Km(DNA)(DNA concentration at the half-maximal rate of ATP hydrolysis) for the reactions werecalculated. Km(ATP)was determined at a saturating concen-tration (1 lm) of ssDNA, and ... [9].Psychrophilic and psychrotrophic bacteria fromAntarctica have evolved various novel adaptive featuresthat allow them to survive and grow at a very low temperature [10–14]. A molecular understanding ... proteins at various temperatures, it appears that,by and large, the in vitro assay temperatures (4 and 25 °C) do not affect the binding (Fig. 5C). Table 2summarizes the key biochemical and biological...
... excessiveattenuation loss atlow temperatures (0˚C to -40˚C). Subsequent research also reveals that theissue becomes more severe for cables exposed to initially high temperatures (greater than ... ADC's own data, engineers set out to pinpoint and formulate a preventative process for dealing with the issue in the field.Dramatic Attenuation in Fiber Access Terminals at Low Temperatures ... paper that outlined similar problems withcertain fibers in varying temperature environments. The paper, entitled "Time- and Temperature- Dependent Material Behavior and Its Impact on Low- Temperature...
... the difference between bkcat and kcat at high pH is large relative to the difference atlow pH, and the difference between bkcat at high pH and bkcat at low pHis relatively large (Fig. 5). ... panel). Atlow pH, bkcatis larger thankcat, but athigh pH bkcatis smaller than kcat.Thatistosay,A328W goes from substrate activation, atlow pH, tosubstrate inhibition, athigh pH. ... bkcat at high pH becomes closer to its value at low pH as the size of the residue at position 328 gets larger(Fig. 2). For example, the difference between bkcat at high pH and bkcat at low...
... spectroscopic and pro-teolysis data, which indicate that apoMb at pH 2.0 ismostly in a random coil conformation, lacks specifictertiary interactions, and displays an extended confor-mation with ... metabolism and pH during globalcerebral ischemia and reperfusion in rats: correlation of1H and 31P NMR spectroscopy with fatty acid and excitatory amino acid levels. J Cereb Blood Flow Metab12, ... path-lengthcuvette (2 mL) was used for CD measurements. The proteinsamples were incubated at the desired denaturant concen-tration for 5 h at 25 °C in order to attain equilibrium.Denaturation...
... obser-vations are not included in this analysis. The SPRF=0wascalculated using estimates of natural mortality, weight at age, and maturity at age. Maturity and weight at age wereusually estimated ... pop-ulation numbers and fishing mortality were usually esti-mated using sequential population analysis (SPA) ofcommercial and (or) recreational catch at age data for most marine populations. ... and related methodsthat reconstruct population size from catch at age data (seeHilborn and Walters (1992, chaps. 10 and 11) for a descrip-tion of the methods used to reconstruct the population...
... fact that the elec-tron donating ability of NH3is higher than those of ethanol and methanol. One is due to the higher electronegativity of the oxy-gen atom than that of the nitrogen atom. ... ppm NH3forthree times at room temperature. It is clear that the response and recovery characteristics are almost reproducible and ratherquick when exposed to NH3 and also when exposed again ... mechanism of low- temperature ammonia oxidation on metal oxides according to thedata of spectrokinetic measurements, Kinet. Catal. 43 (2002) 363–371.[28] J. Watson, The tin oxide gas sensor and its...
... sen-sors at temperatures low enough so that appreciable structuralvariation never occurs, provided that gas reactions occur at areasonable rate.Additionally, the development of sensors that work at ... operated at room temperature. These sensorswere found to be insensitive to NO2, CO and NH3 at low temper-atures of operation. Additionally, Sberveglieri and co-workers[17] reported that, ... paper)were irresponsive to gases when operated at temperatures below350◦C. Finally, it is well known that pure SnO2-based sensorsare almost insensitive to gases when operated at room tempera-ture...
... by a heat treatment in air at 520 8C. Sample TiO2-NT3 was prepared by heat treatment in Ar at 700 8C followed by a heat treatment in air at 520 8C.XRD analysis showed that the heat treatment ... of the photocatalysts for propene oxidation at low concentrationThe photocatalytic performance of these new materials wasinvestigated for the oxidation of propene atlow concentration,100 ppmv, ... argon at higher temperatures, theanatase was transformed into rutile either partially (at 550 8C) orcompletely (at 700 8C). Sample TiO2-NT2 was prepared by heattreatment in Ar at 550 8C followed...
... gainst denaturant c oncentration.Fig. 5. Far UV-CD spectra of stem bromelain. Native protein at pH 7.0 (ÐÁÐ), acid-induced state at pH 2.0 (Ð) and 6MGdnHCldenatured state (± ±).50 S. Khatun Haq ... conformational state that isdifferent from the native state at pH 7.0 as well ascompletely unfolded state in the presence of 6MGdnHCl.Figure 3 shows the near UV CD spectra of the nativestate ... lessconstant, indicative of the presence of a non-native stableintermediate atlow pH.The emission spectrum of stem bromelain at pH 7.0(Fig. 2) shows a maximum at 347 nm that suggests thatsome of...
... adequately described as a two-state processwithout the accumulation of intermediates at neutral and moderately basic conditions (pH 7–9) at 25 °C. The stabilityof the protein, at pH 7.5 and ... suggesting that proteinstability was similar in that pH range.It is interesting to note here that the transition at pH 3.5(Fig. 5A) showed a low degree of cooperativity at low temperatures, although ... thermal denatur-ation experiments, we decided to follow the chemicaldenaturation at pH 7.5 to compare with the stabilityFig. 5. Thermal denaturation profiles of HPr followed by far-UV CD at 222...
... of lowtemperature of RFA at the target sites onlung metastasis of hepatic VX2 carcinomaWe examined by gross observation lung metastasis ofhepatic VX2 carcinoma after RFA atlow temperature. Quantifiable ... HCC. The real ablative temperature ofthe tumor might be nearer to the target temperature, compared with the clinical situation. This was why wechose 85°C as the highest RFA temperature. At present, ... invasion and metastasis when the local ablative temperature wasnot sufficiently h igh. Besides, it seemed that the lowerthe target temperature was, the more significant werethe local proliferation...