rapa the rna polymerase associated homolog of the swi snf protein superfamily

Báo cáo khoa học: The SWI⁄SNF protein BAF60b is ubiquitinated through a signalling process involving Rac GTPase and the RING ﬁnger protein Unkempt doc

... these transcription factors and SWI ⁄ SNF complexes, thereby allowing the recruitment of SWI ⁄ SNF to target genes [5–9] Biochemical puriﬁcation and analysis of SWI ⁄ SNF complexes have revealed ... process, the molecular composition of the E3 ligase involved and the role of Unkempt RING ﬁnger remain uncertain On the basis of the results of a mutational analysis (Figs and S1), it appears that the ... However, the mechanisms leading to ubiquitination of speciﬁc SWI ⁄ SNF subunits and their regulation have not been molecularly deﬁned Ubiquitination consists of the covalent attachment to proteins of...

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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot

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... activation of these genes within the same time window The activator-binding domains in the Swi1 and Snf5 subunits of the SWI ⁄ SNF complex are essential for recruitment of the SWI ⁄ SNF complex to these ... targets of SWI ⁄ SNF Before we could test the signiﬁcance of the Swi1 and Snf5 activator interaction domains, it was necessary to investigate whether the selected genes were direct targets of the SWI ... ORFs of SWI1 and SNF5 are both deleted, because deletion of these genes has been shown to disrupt the integrity of the SWI ⁄ SNF complex [39,40] The most appropriate time for revealing SWI ⁄ SNF...

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Báo cáo sinh học: "Imp-L2, a putative homolog of vertebrate IGF-binding protein 7, counteracts insulin signaling in Drosophila and is essential for starvation resistance" ppt

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... vertebrates, most of the circulating IGFs are part of ternary complexes consisting of an IGF, IGFBP-3 and ALS [29] These ternary complexes prolong the half-lives of the IGFs and restrict them to the vascular ... including Journal of Biology 2008, 7:10 10.8 Journal of Biology 2008, Volume 7, Article 10 Honegger et al the controlled expression of binding partners of the extracellular ligands Surprisingly, the well-characterized ... carbohydrate levels in the larval hemolymph [18,19], reminiscent of the phenotypes of starved or IISimpaired flies The Drosophila genome does not encode an obvious homolog of the IGFBPs Furthermore, genetic...

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Báo cáo y học: "The SET -domain protein superfamily: protein lysine methyltransferase" docx

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... the first SET-domain protein lysine methyltransferases to be characterized, following the discovery of sequence homology between their SET domains [13] These proteins, with other members of the ... third of the protein The GLP1 gene has 25 exons - it lacks homologs of the first three introns of G9a - and the 20 exons from the 3Ј end have identical junctions to those found in G9a The GLP ... regions of the first and the last exons (these are shown to scale with the length of the exons except where lines are broken) (a,c) Protein structures are shown on the same scale as the coding...

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Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot

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... interaction could be mediated by another protein, or other regions of RNA pol II Bulky DNA lesions block the passage of elongating RNA pol II, thereby stalling the transcription apparatus [43] To ... overlapped with the sites of RNA synthesis [58,59] Another example for prominent replication factors that interact with RNA pol II are the MCM proteins [10] MCM proteins can be detected in RNA pol II ... phosphatase treatment, the cellular fraction of RNA pol II that associated with Pol e showed increased mobility, corresponding to that of isoform IIA The isoform IIO was also lost from RNA pol II precipitates...

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Báo cáo khóa học: Three cyclin-dependent kinases preferentially phosphorylate different parts of the C-terminal domain of the large subunit of RNA polymerase II potx

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... maximum of 6–20% of the GST-CTD molecules could be phosphorylated over the course of the reaction It is therefore unlikely that the observed generation of the IIo band was a consequence of limiting ... also need to mention the mobility of the IIo forms of the different substrates The IIo form of the N-terminal 1–15 repeats was only slightly retarded relative to the position of the unphosphorylated ... possible action of the three kinases on the C-terminal domain (CTD) The amino acid sequence of the mouse CTD is shown at the bottom In the diagram, the heptad repeats, with N at position of the YSPTSPS...

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Báo cáo khoa học: Mode of action of the microbial metabolite GE23077, a novel potent and selective inhibitor of bacterial RNA polymerase docx

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... stages of the transcription process, for example (a) lipiarmycin inhibits the formation of the ﬁrst dinucleotide of the nascent RNA chain [11], (b) rifampicin blocks the synthesis of RNA molecules ... coli RNAP, the possibility was investigated that the compound might inhibit RNA synthesis by preventing RNAP from binding to DNA Binding of RNAP to DNA is indeed one of the earliest steps of the ... totally inhibited RNA synthesis when added before DNA, whereas Fig Eﬀect of GE23077 and other RNA polymerase (RNAP) inhibitors on in vitro RNA synthesis: comparison of the eﬀects of compound addition...

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Báo cáo sinh học: " Open Access Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" docx

Danh mục: Hóa học - Dầu khí

... into the cytoplasm and subsequently enter the nucleus where replication of the vRNA results in the production of both mRNA and complementary RNA (cRNA), the template for synthesis of more vRNA ... from the start of the 18S rRNA gene sequence The resulting MDCK clone then would be tested for RNA pol I promoter activity in vitro The sequence of the region upstream of the canine 18S rRNA initiation ... from other mammalian species In the genomes of human, mouse and rat, the distance from the beginning of the 18S rRNA sequences to Page of 12 (page number not for citation purposes) Virology Journal...

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Báo cáo hóa học: " Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" pdf

Danh mục: Hóa học - Dầu khí

... into the cytoplasm and subsequently enter the nucleus where replication of the vRNA results in the production of both mRNA and complementary RNA (cRNA), the template for synthesis of more vRNA ... from the start of the 18S rRNA gene sequence The resulting MDCK clone then would be tested for RNA pol I promoter activity in vitro The sequence of the region upstream of the canine 18S rRNA initiation ... from other mammalian species In the genomes of human, mouse and rat, the distance from the beginning of the 18S rRNA sequences to Page of 12 (page number not for citation purposes) Virology Journal...

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Báo cáo hóa học: " The use of RNA-dependent RNA polymerase for the taxonomic assignment of Picorna-like viruses (order Picornavirales) infecting Apis mellifera L. populations" pptx

Danh mục: Hóa học - Dầu khí

... HaRNAV Picornaviridae Picornaviridae Picornaviridae Picornaviridae Picornaviridae Picornaviridae Picornaviridae Picornaviridae Picornaviridae Picornaviridae Picornaviridae Picornaviridae Picornaviridae ... separated the family Picornaviridae from the others The amino acid Glycine (G) was nonetheless found to be conserved amongst all of the members With the exception of the genus Ilfavirus, all members of ... replicase polyprotein and also with the order of these domains [6] The newly defined order Picornavirales, often referred to as the Picorna-like superfamily, encompasses the families Picornaviridae,...

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Báo cáo khoa học: " Avian reovirus L2 genome segment sequences and predicted structure/function of the encoded RNA-dependent RNA polymerase protein" docx

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... Journal 2008, 5:153 Background The avian reoviruses (ARVs) are members of the family Reoviridae, the only group of dsRNA viruses (out of seven dsRNA virus families) that infect mammals [1,2] The ... Differences in the functional properties of homologous ARV and MRV proteins have also been reported For example, two non-homologous dsRNA-binding proteins (the ARV σA core protein and the MRV σ3 ... help tether the RdRp to the underside of the core shell This hypothesis could be tested by extending such ARV:MRV:AqRV sequence comparisons to the other core proteins In conclusion, we report the...

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THE ROLE OF THE CTD PHOSPHATASE RTR1 AND POST-TRANSLATIONAL MODIFICATIONS IN REGULATION OF RNA POLYMERASE II

Danh mục: Y khoa - Dược

... Given the importance of the activity of dephosphorylation of the RNAPII, it is expected that defects of these regulatory genes have dire consequences One prominent example is the human homolog of ... Figure Model of regulation of phosphorylation of the RNA Polymerase II CTD during transcription elongation in S cerevisiae The roles of the three known protein phosphatases are hypothesized as ... L Cox THE ROLE OF THE CTD PHOSPHATASE RTR1 AND POST-TRANSLATIONAL MODIFICATIONS IN REGULATION OF RNA POLYMERASE II RNA polymerase II (RNAPII) is regulated by multiple modifications to the Cterminal...

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MECHANISMS OF RECRUITMENT OF THE CTD PHOSPHATASE RTR1 TO RNA POLYMERASE II

Danh mục: Y khoa - Dược

... facilitate cleavage of the fusion protein N-terminal to the Protein A portion of the epitope tag resulting in elution of the protein of interest from the IgG resin Following preparation of the yeast cell ... introduce the TAP tag to the protein of interest through the direct incorporation of the C-terminal TAP tag sequence into the chromosomal locus of the protein- encoding gene of interest The work ... variety of mechanisms, one of which involves the recruitment of specific sets of RNA processing factors to specific modified forms of the C-terminal domain (CTD) of Rpb1, the largest subunit of RNAPII,...

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Nhp6p and med3p regulate gene expression by controlling the local subunit composition of RNA polymerase II

Danh mục: Cao đẳng - Đại học

... in RNA splicing RNA polymerase III is needed for the synthesis of transfer RNA (tRNA) and other small nuclear RNAs (including the small 5S rRNA) (Lewin, 2004) RNA polymerase II (also called RNA ... ribosomal RNA (rRNA), which is processed into 28S, 5.8S and 18S rRNA RNA polymerase II catalyzes the transcription of DNA to synthesize the precursors of mRNA and four of the five small nuclear RNAs ... complexes depending on the type of ATPase subunit present in the complex: SWI/ SNF and ISWI The ATPase activity of the SWI/ SNF complex is preferentially stimulated by naked DNA The SWI/ SNF complex remodels...

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Báo cáo khoa học: Effect of oculopharyngeal muscular dystrophy-associated extension of seven alanines on the ﬁbrillation properties of the N-terminal domain of PABPN1 pot

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... consisting of amino acids 1–125 of the wild-type protein (N-WT) Because the aim of this study was to investigate the effect on the ﬁbrillation properties of the most extreme disease -associated ... segment [13,14] The poly l-alanine extensions affect the N-terminal fragment of the protein, which comprises 125 amino acids In the wild-type protein, the sequence (Ala)10Gly(Ala)2 follows the start ... e.g of the NM domain of the yeast prion protein Sup35p [29] and also for the detection of a-synuclein ﬁbrils [30] In fact, ANS signals showed a linear correlation with the concentration of ﬁbrils...

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Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx

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... medium [20], the capacity of the cell to synthesize the different metabolic precursors (GDP-Man, UDP-Glc, Man-P-Dol, Glc-P-Dol), and the variations in the level of expression of the various glycosytransferases, ... correlated with the decrease in the percentage of radioactivity associated with oligomannoside-type glycans, mainly the Man9 population If we look at the end products of processing, most of the Man5GlcNAc2 ... cells, the monoglucosylated species detected on glycoproteins could only originate from the action of UGGT Furthermore, when the incubation was performed in the presence of 20 lM kifunensine, the...

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Báo cáo khoa học: Spectroscopic characterization of the isolated heme-bound PAS-B domain of neuronal PAS domain protein 2 associated with circadian rhythms doc

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... in various functions of PAS proteins, including the interaction of NPAS2 with BMAL1 The PAS domain is critical for the ability of PAS proteins to bind other proteins [27,28] The small chemical ... 413 cm)1) of the Fe(II)–CO complex of isolated PAS-B in the low (left) and high (right) frequency regions The lower spectra are of the Fe(II)–12C16O complexes, the middle spectra are of the Fe(II)–13C18O ... et al make up part of the core of the PAS-B domain [27,28] Therefore, the lack of these residues in the study by Dioum et al may have led to instability of their protein [14] Dioum et al [14]...

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Báo cáo khoa học: Identiﬁcation of mitogen-activated protein⁄extracellular signal-responsive kinase kinase 2 as a novel partner of the scaffolding protein human homolog of disc-large docx

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... that the PDZ2 domain of hDlg and the C-terminal RTAV sequence of MEK2 are required for the optimal interaction of the two protein partners Coimmunoprecipitation of endogenous hDlg and MEK2 proteins ... outside the vascular system, including the brain, liver and heart, have initially revealed the existence of several isoforms of the hDlg protein, containing various combinations of alternatively ... in their target proteins [8] On the basis of site-directed mutagenesis of both interactants, we conﬁrmed the major involvement of the PDZ-2 domain of hDlg in the interaction with the C-terminal...

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Báo cáo Y học: The DNA-polymerase inhibiting activity of poly(b-L-malic acid) in nuclear extract during the cell cycle of Physarum polycephalum pot

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... DNA polymerases a and e [13], and DNA polymerase d [14] were consistent with the recovery of > 95% of DNA polymerase a and > 75% of the other DNA polymerases in the extract Standard DNA polymerase ... whether the intrinsic DNA polymerase activity of the extract varied during the cell cycle or whether it was totally modulated by the degree of complex formation of DNA polymerases with PMLA The ... to vi and vo The expression for the relative inhibitory activity is then (vo ) vi)/vi ¼ [PMLA]/Ki Therefore, the higher the concentration of free PMLA, and the lower the value of the inhibition...

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Báo cáo sinh học: "Bisphosphonate-associated osteonecrosis of the jaw is linked to suppressed TGFb1-signaling and increased Galectin-3 expression: A histological study on biopsies" pot

Danh mục: Hóa học - Dầu khí

... from the lower jaw The gender and age of patients were matched in the BRONJ and control groups, except the samples from the orthognatic surgery procedure The average age of the patients in the ... immunohistochemical methods The observers were blinded to the tissue origin of the visual fields The qualifaction of the observers were dentist (1) and physician (2) engaged in their dental/medical thesis dealing ... tissues, the overexpression of TGFb1 causes an arrest of the EMT process in activated myofibroblasts; conversely, in BRONJ, the lack of TGFb1 and Smad-2/3 activity attenuated the stimulation of EMT...

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