radioimmunoassay and enzyme linked immunosorbent assay for calbindin d28k
ELISA - Enzyme-Linked Immunosorbent Assay
... goat anti-mouse Conjugated Enzyme ex: goat anti-mouse with HRP Substrate (enzyme conversion) ex: DAB Antigen ELISA for Antibody Detection (Enzyme- Linked Immunosorbent Assay) • • • • • • • • • ... to wells Washs Add 2nd antibody (attach enzyme) Washes Color development Ex: HIV test Sandwich ELISA for Antigen Detection (Enzyme- Linked Immunosorbent Assay) • • • • • • • Capture 1st antibody ... animal is harvested for B cells Fusion of specific B cell with tumor cells to create hybridomas (immortal B cells) Screen for antibody producing hybridomas Culture hybridomas and purify specific...
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KỸ THUẬT ELISA (ENZYME-LINKED IMMUNOSORBENT ASSAY) TRONG XÁC ĐỊNH CHỦNG MYCOBACTERIA VÀ TRONG CHẨN ĐOÁN SÀNG LỌC BỆNH LAO I. NGUYÊN TẮC: Kỹ thuật ELISA potx
... KỸ THUẬT ELISA (ENZYME- LINKED IMMUNOSORBENT ASSAY) TRONG XÁC ĐỊNH CHỦNG MYCOBACTERIA VÀ TRONG CHẨN ĐOÁN SÀNG LỌC BỆNH LAO I NGUYÊN ... nhiễm bệnh thể Phức hợp kháng nguyên-kháng thể nhận biết cộng hợp kháng huyết tương ứng gắn với enzyme peroxidaza phosphataza… gây chuyển mầu chất phù hợp Kết phản ứng đo thiết bị đo quang phổ...
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Báo cáo khoa học: " Estimation of Paratuberculosis Prevalence in Dairy Cattle in a Province of Korea using an Enzyme-linked Immunosorbent Assay: Application of Bayesian Approach" ppt
... newly developed and employed once without gold standard the prior information on the sensitivity and specificity, denoted by η and θ, respectively, was primarily formed using the information obtained ... commercial enzyme- linked immunosorbent assay for J ohnes disease J Clin Microbiol 1991, 29(2), 272-276 Cox, J C., Drane, D P , Jones, S L., Ridge, S and Milner, A R Development and evaluation ... Posterior medians and 95% credible intervals of the sensitivity and specificity by three different priors of prevalence (one for main prior and two for extreme prior) were given in Table and Sensitivity...
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báo cáo hóa học:" Evaluation of the tuberculin skin test and the interferon-γ release assay for TB screening in French healthcare workers" ppt
... 33 34 35 36 National Institute for Health and Clinical Excellence: Tuberculosis: clinical diagnosis and management of tuberculosis, and measures for its prevention and control London 2006 Zellweger ... the forearm and the transverse diameter of the induration was read after 72 to 96 hours [19] A diameter ≥ 10 mm was considered positive Before TST application, the interview was performed and ... study and was involved in data collection and writing of the paper EC was involved in data collection and gave critical comments for manuscript writing PG was involved in data collection and gave...
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Báo cáo y học: "Comparison of metal-dependent catalysis by HIV-1 and ASV integrase proteins using a new and rapid, moderate throughput assay for joining activity in solution" ppt
... this case, optima for ASV IN were in the range of pH 8–8.5 for both processing and joining, whereas the optima for HIV-1 IN were substantially lower, pH for processing and pH 6.5 for joining, although ... sensitive than the standard gel assay for measuring this activity In addition, the assay is much faster than gel analysis and numerous samples can be handled with relative ease The assay described ... and data analysis, and contributed to writing and editing the manuscript JC designed the assay and performed some of the preliminary experiments GM conducted all of the optimization studies and...
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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens
... Aboujaoude, E.N., Cheon, Q and Ames, B.N (1994) Assays of oxidative DNA damage biomarkers 8-oxo-2-deoxyguanosine and 8-oxoguanine in nuclear DNA and biological fluides by high-performance liquid chromatography ... in S Typhimurium TA strains YG1041 and YG1042 have 50-100 times respectively higher levels of both NR and OAT enzyme activity than their host strains TA98 and TA100 (Hagiwara et al 1993) We compared ... (1041, 1042) and their host TA strains (98, 100) 2-Nitrofluoranthene, 1-nitropyrene and 1,8-dinitropyrene are tested without S9mix, Glu-P-1 and Trp-P-2 are tested with S9mix using TA98 and YG1041...
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Florian Kajuth und Sebastian Watzka: Inflation expectations from index-linked bonds: Correcting for liquidity and inflation risk premia docx
... in (7) and IPt is a measure for the inflation risk premium, and εt is assumed normally distributed whited noise Daily data for spreadt and LPt are taken from the Cleveland Fed homepage and run ... BEIR for both premia The Federal Reserve Bank of Cleveland publishes an adjusted measure for expected inflation each month For May 2008 the Cleveland Fed puts expected inflation after adjustment for ... yields and expected inflation and presents estimation results for the adjusted values for expected inflation Finally section concludes TIPS are linked to the urban not-seasonally adjusted U.S CPI For...
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Báo cáo khoa học: The potyviral virus genome-linked protein VPg forms a ternary complex with the eukaryotic initiation factors eIF4E and eIF4G and reduces eIF4E affinity for a mRNA cap analogue ppt
... like to thank Genevieve Roudet for her skilful assistance, and Dr T Candresse and Dr T Delaunay for stimulating discussions Many thanks to Professor K S Browning for providing us with eIF4G polyclonal ... ice for (1 s cycles) The crude extract was centrifuged at 20 000 g, °C for 30 The supernatant was recovered and centrifuged at 100 000 g, °C for 45 The clarified supernatant was incubated for ... (0.5 mgÆmL)1) was added, and the suspension was incubated for 45 at °C with gentle stirring DNase and RNase (100 lg each) were added and the suspension was incubated at °C for another 45 The lysate...
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Báo cáo khóa học: Modified colorimetric assay for uricase activity and a screen for mutant Bacillus subtilis uricase genes following StEP mutagenesis pptx
... extracts and affinity chromatography of purification and an activity assay by spectrophotometry We have demonstrated the usefulness of this assay and used it to screen for a mutant uricase enzyme ... treated at 60 °C for h for cell lysis and release of the protein Following the addition of substrate, the plate was incubated for 10 at 37 °C, a bright purple color was observed and absorbance ... medium plates are incubated at 37 °C for a maximum of 18 h with shaking to induce the expression of mutant uricase and then treated for 60 °C for h to cause lysis and release of the uricase protein...
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Báo cáo hóa học: " Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood" pptx
... required upon hand-off for assay validation SJ, AW, SWA and KA performed the in vitro assays on monkeys treated with Ab-01 and control Ig and analyzed the data, and KA and SA performed the experiments ... "inventoried" assays available from ABI, and are described in Table cDNA synthesis, preparation of samples for TLDA assay and measurements of RNA concentration were performed as described for the human assay ... administration and minutes post dosing RNA isolation, description of custom TLDA and assay of RNA concentration for monkey studies RNA isolation was performed as described above for the human blood assay...
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Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
... of plasmid DNA for GI NoV and × 107 to × 101 for GII NoV The R2 values for both standard curves were 1.00 with a slope of -3.5 and -3.7 respectively for GI and GII NoV (Fig 1B and Fig 2B) Melting ... 94°C for min, followed by 40 cycles at 94°C for min, 45°C for min, 68°C for and a final extension at 68°C for The PCR for GII was a denaturation at 94°C for min, followed by 40 cycles at 94°C for ... to 101 for GI and × 107 to × 101 for GII (Fig 1A and Fig 2A) Melting curve analysis identified positive samples by large peaks at ~90°C and ~88°C respectively for GI and GII NoV (Fig 1C and Fig...
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Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx
... the Standard curves cellular gene assays RV2 rhadinovirus and Standard curves obtained from the RV2 rhadinovirus and OSM reference cellular gene assays A) The standard RV2 assay was performed ... concentrations were 4.0 mM for the RV2 assay and 2.0 mM for the OSM assay After activation of the polymerase by incubation for minute at 95°C, amplification was performed on a BioRad iCycler ... on a BioRad iCycler equipped with an optical module for 45 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 30 s The copy number for each assay was calculated from the cycle threshold (CT)...
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Báo cáo sinh học: " Shell Vial culture Assay for the rapid diagnosis of Japanese encephalitis, West Nile and Dengue-2 viral encephalitis" pdf
... of WN and Dengue-2 infections in Pondicherry and surrounding areas [7] Various techniques have been tried, standardized, evaluated for the laboratory diagnosis of infections due to JE, WN and Den-2 ... by serology for Japanese encephalitis and Dengue viruses [9] Isolation in cell culture, though cumbersome, time consuming and less sensitive is still considered the gold standard for the diagnosis ... ensure a rapid and precise diagnosis, early, prompt collection, transport and processing of the CSF samples becomes mandatory Conclusion Shell vial culture is a rapid and efficient tool for the etiological...
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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
... 350714 and Y = × 106X + × 107 and the r2 were 1.0 and 0.95 for JCV(Mad1) virus stocks I and II, respectively Page of (page number not for citation purposes) Virology Journal 2006, 3:3 and inter-run ... responsible for red blood cell (RBC) agglutination and traditionally, hemagglutination (HA) and HA inhibition (HAI) assays have been employed for quantitation of JCV [8-14] However, the HA assay is ... blood-brain-barrier and lytic infection of oligodendrocytes, are still unclear Efforts to understand the pathogenesis of PML have been hampered by the lack of standard methods for JCV detection and quantitation...
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Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt
... of plasmid DNA for GI NoV and × 107 to × 101 for GII NoV The R2 values for both standard curves were 1.00 with a slope of -3.5 and -3.7 respectively for GI and GII NoV (Fig 1B and Fig 2B) Melting ... 94°C for min, followed by 40 cycles at 94°C for min, 45°C for min, 68°C for and a final extension at 68°C for The PCR for GII was a denaturation at 94°C for min, followed by 40 cycles at 94°C for ... to 101 for GI and × 107 to × 101 for GII (Fig 1A and Fig 2A) Melting curve analysis identified positive samples by large peaks at ~90°C and ~88°C respectively for GI and GII NoV (Fig 1C and Fig...
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báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot
... the Standard curves cellular gene assays RV2 rhadinovirus and Standard curves obtained from the RV2 rhadinovirus and OSM reference cellular gene assays A) The standard RV2 assay was performed ... concentrations were 4.0 mM for the RV2 assay and 2.0 mM for the OSM assay After activation of the polymerase by incubation for minute at 95°C, amplification was performed on a BioRad iCycler ... on a BioRad iCycler equipped with an optical module for 45 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 30 s The copy number for each assay was calculated from the cycle threshold (CT)...
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báo cáo hóa học:" Shell Vial culture Assay for the rapid diagnosis of Japanese encephalitis, West Nile and Dengue-2 viral encephalitis" pdf
... of WN and Dengue-2 infections in Pondicherry and surrounding areas [7] Various techniques have been tried, standardized, evaluated for the laboratory diagnosis of infections due to JE, WN and Den-2 ... by serology for Japanese encephalitis and Dengue viruses [9] Isolation in cell culture, though cumbersome, time consuming and less sensitive is still considered the gold standard for the diagnosis ... ensure a rapid and precise diagnosis, early, prompt collection, transport and processing of the CSF samples becomes mandatory Conclusion Shell vial culture is a rapid and efficient tool for the etiological...
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báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx
... 350714 and Y = × 106X + × 107 and the r2 were 1.0 and 0.95 for JCV(Mad1) virus stocks I and II, respectively Page of (page number not for citation purposes) Virology Journal 2006, 3:3 and inter-run ... responsible for red blood cell (RBC) agglutination and traditionally, hemagglutination (HA) and HA inhibition (HAI) assays have been employed for quantitation of JCV [8-14] However, the HA assay is ... blood-brain-barrier and lytic infection of oligodendrocytes, are still unclear Efforts to understand the pathogenesis of PML have been hampered by the lack of standard methods for JCV detection and quantitation...
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báo cáo hóa học:" Tuberculosis and HIVNeeded: A New Paradigm for the Control and Management of Linked Epidemics" pot
... fragmented, uncoordinated, and unsuccessful It is essential, in areas of high HIV prevalence and TB burden, that national TB and HIV programs collaborate and that care for TB/HIV co-infected individuals ... various collaborative and integrative efforts at the healthcare delivery level in urban and rural areas have been carried out or are ongoing In Rwanda, integration of TB and HIV services at a ... Antiretrovirals Ol Rx and Px Adherence Support Community Support HIV Prevention Integration of Services Figure Common and alternative TB and HIV program paradigms Common and alternative TB and HIV program...
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