... 2080 ofthe NL4-3, NL- 913 and NL1907 genomic RNAs and nt 456 to 2037 ofthe NLΔSL1, NLΔSL1- 913 and NLΔSL1 -19 07 genomic RNAs were used for the folding predictions QuantitativePCRmeasurementofRNA ... 2000, 3 01: 4 91- 511 Page 11 of 12 17 Berkowitz R, Fisher J, Goff SP: RNA packaging Curr Top Microbiol Immunol 19 96, 214 :17 7- 218 18 Freed EO: HIV -1 gag proteins: diverse functions in thevirus life ... targeting cellular Y1, Y3 and SRP RNAThe amount of cellular RNA in the NL4-3 virion was set at 10 0% Copy numbers for Y1, Y3 and SRP RNA were 275-362, 1, 710 2,006 and 1.1 × 10 6 -1. 3 × 10 6, respectively,...
... study, 11 8 HIV -1- infected and 18 2 HIV1-uninfected, the infection was confirmed by disease onset and/or persistence of HIV -1 antibody after 18 months of age, while lack of infection was confirmed by ... categorizing mothers according to low (< 10 00 HIV -1 RNA copies/ml of plasma) and high (> 10 00 HIV -1 RNA copies/ml of plasma) viral load As for the larger cohort, while neither ofthe two SNPs was ... risk of MTCT of HIV -1 compared to the most frequent [G;A] and [A;G] haplotypes (Table 1) Values of maternal viral load at delivery were available in a subgroup of 10 9 infants (median 8790 HIV -1 RNA...
... Sodroski J, Springer TA: The lymphocyte chemoattractant SDF -1 is a lig- Page of (page number not for citation purposes) Virology Journal 2005, 2:9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 and ... 2.5 15 IM28 (ug/ml) Figure IM28 on fusion of TF228 .1. 16 cells with SupT1 cells Effect of3 Effect of IM28 on fusion of TF228 .1. 16 cells with SupT1 cells TF228 .1. 16 cells were mixed with SupT 11 cells ... HIV -1 infection, and SupT1 cells [15 ,16 ] The fusion of 293/CD4+ cells with TF228 .1. 16 cells was completely inhibited by a lower dose of IM28 than was the fusion of SupT1 cells with TF228 .1. 16...
... Interaction of HIV -1 and human salivary mucins J Acquir Immune Defic Syndr 19 94, 7:995 -10 02 Page of 10 (page number not for citation purposes) Virology Journal 2008, 5:59 10 11 12 13 14 15 16 17 18 19 ... aggregation ofthevirusbythe mucins, leaving no free viruses to pass through the filter paper into the filtrate to cause viral infection On the other hand the 10 0% infection ofthe CEM-SS cells ... or compare the efficiency of HIV -1 aggregation bythe crude pregnancy plug mucus and purified pregnancy plug mucins, at the end ofthe incubation period (1 h), the mixtures of (HIV -1 plus crude...
... Sodroski J, Springer TA: The lymphocyte chemoattractant SDF -1 is a lig- Page of (page number not for citation purposes) Virology Journal 2005, 2:9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 and ... 2.5 15 IM28 (ug/ml) Figure IM28 on fusion of TF228 .1. 16 cells with SupT1 cells Effect of3 Effect of IM28 on fusion of TF228 .1. 16 cells with SupT1 cells TF228 .1. 16 cells were mixed with SupT 11 cells ... HIV -1 infection, and SupT1 cells [15 ,16 ] The fusion of 293/CD4+ cells with TF228 .1. 16 cells was completely inhibited by a lower dose of IM28 than was the fusion of SupT1 cells with TF228 .1. 16...
... instructions Page 13 of 14 10 11 12 13 14 15 Acknowledgements We would like to thank the members ofthe Kashanchi lab for experiments and assistance with the manuscript This work was partly funded by AI043894, ... mammalian RNA polymerase II carboxyl-terminal domain Proc Natl Acad Sci USA 19 93, 90 :11 167 -11 1 71 Zhang J, Corden JL: Phosphorylation causes a conformational change in the carboxyl-terminal domain ofthe ... Selectivity Name ACH2 J1 .1 OM10 .1 U1 CEM Infected Jurkat U937 Uninfected Alsterpaullone (10 μM) 11 25 15 37 89 92 88 Indirubin-3’-monoxime Indirubin-3’-monoxime-5’-indo (10 μM) (10 μM) 22 24 32 35...
... (B), D51Q (C) and the wild type CAp24 (D) Bars indicate 10 0 nm tion ofthe D51E mutant was reduced by 2- to 6-fold as compared to the wild typeThe effect ofthe three CAp24 mutations on virus ... digestion The resulting plasmid was designated pET11a-CA and verified by sequencing The three HIV -1 CAp24 mutants, D51N, D51E, and D51Q, in the pET11a-CA vector were then engineered by site-directed ... Productive HumanImmunodeficiencyVirusType Assembly Takes Place at the Plasma Membrane 2007, 81( 14):7476-7490 Kaplan AH, Manchester M, Swanstrom R: The activity ofthe protease ofhuman immunodeficiency...
... 2000, 1( 1): 31- 39 Gaus K, Chklovskaia E, Fazekas de St Groth B, Jessup W, Harder T: Condensation ofthe plasma membrane at the site of T lymphocyte activation J Cell Biol 2005, 17 1 (1) :12 1 -13 1 Gaus ... phosphorylation-independent downregulation of cell-surface CD4 by nef Nature 19 91, 350(6 318 ):508- 511 Michel N, Allespach I, Venzke S, Fackler OT, Keppler OT: The Nef protein ofhumanimmunodeficiencyvirus establishes ... Sci U S A 19 99, 96 (14 ): 816 7- 817 2 Thoulouze MI, Sol-Foulon N, Blanchet F, Dautry-Varsat A, Schwartz O, Alcover A: Humanimmunodeficiencyvirus type- 1 infection impairs the formation ofthe immunological...
... involvement ofhuman immu- 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 nodeficiency virustype integrase in nuclear import of viral cDNA during acute infection J Virol 2004, 78 :11 563 -11 573 Nakamura ... following the first PCR, a second PCR was carried-out to amplify a portion ofthe HIV -1 LTR sequence from the first Alu-LTR PCR- amplified products The first PCR was carried out by Page 13 of 15 (page ... confirmed by Petit and colleagues by studying the intracellular localization of HIV -1 Flag-IN [18 ], other studies, using GFP-IN fusion protein, did not reveal the importance of K186Q and Q 214 / 216 L...
... enhancement of particle budding: role ofthe cytoplasmic domain J Virol 19 96, 70:2669-2673 Page 10 of 11 (page number not for citation purposes) Virology Journal 2007, 4: 81 10 11 12 13 14 15 16 17 18 19 ... 20 01: 19 71- 20 41 Strebel K, Klimkait T, Martin MA: A novel gene of HIV -1, vpu, and its 16 -kilodalton product Science 19 88, 2 41: 12 21- 1223 Bour S, Strebel K: Thehumanimmunodeficiencyvirus (HIV) type ... recruits other proteins such as Skp1, Cul -1 and the Cdc34 E2 ubiquitin ligase [11 ] This results in ubiquitination of CD4, its dislocation from the ER and degradation bythe proteosome [12 ,13 ] The stable...