quantitative measurement of human immunodeficiency virus type 1 rna by using the roche amplicor pcr kit
Báo cáo y học: "Mutations in matrix and SP1 repair the packaging specificity of a Human Immunodeficiency Virus Type 1 mutant by reducing the association of Gag with spliced viral RN" ppsx
... 2080 of the NL4-3, NL- 913 and NL1907 genomic RNAs and nt 456 to 2037 of the NLΔSL1, NLΔSL1- 913 and NLΔSL1 -19 07 genomic RNAs were used for the folding predictions Quantitative PCR measurement of RNA ... 2000, 3 01: 4 91- 511 Page 11 of 12 17 Berkowitz R, Fisher J, Goff SP: RNA packaging Curr Top Microbiol Immunol 19 96, 214 :17 7- 218 18 Freed EO: HIV -1 gag proteins: diverse functions in the virus life ... targeting cellular Y1, Y3 and SRP RNA The amount of cellular RNA in the NL4-3 virion was set at 10 0% Copy numbers for Y1, Y3 and SRP RNA were 275-362, 1, 710 2,006 and 1. 1 × 10 6 -1. 3 × 10 6, respectively,...
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Báo cáo hóa học: " Toll-like receptor 9 polymorphisms influence mother-to-child transmission of human immunodeficiency virus type 1" pptx
... study, 11 8 HIV -1- infected and 18 2 HIV1-uninfected, the infection was confirmed by disease onset and/or persistence of HIV -1 antibody after 18 months of age, while lack of infection was confirmed by ... categorizing mothers according to low (< 10 00 HIV -1 RNA copies/ml of plasma) and high (> 10 00 HIV -1 RNA copies/ml of plasma) viral load As for the larger cohort, while neither of the two SNPs was ... For prevention of MTCT of HIV -1, highly active antiretroviral therapy was recently Page of recommended for all women with HIV -1 RNA levels of ≥ 10 00 copies/ml of plasma [13 ] A further analysis...
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Báo cáo sinh học: " Inhibition of human immunodeficiency virus type-1 (HIV-1) glycoprotein-mediated cell-cell fusion by immunor (IM28)" docx
... Sodroski J, Springer TA: The lymphocyte chemoattractant SDF -1 is a lig- Page of (page number not for citation purposes) Virology Journal 2005, 2:9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 and ... 2.5 15 IM28 (ug/ml) Figure IM28 on fusion of TF228 .1. 16 cells with SupT1 cells Effect of3 Effect of IM28 on fusion of TF228 .1. 16 cells with SupT1 cells TF228 .1. 16 cells were mixed with SupT 11 cells ... HIV -1 infection, and SupT1 cells [15 ,16 ] The fusion of 293/CD4+ cells with TF228 .1. 16 cells was completely inhibited by a lower dose of IM28 than was the fusion of SupT1 cells with TF228 .1. 16...
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Báo cáo hóa học: " The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay" ppt
... Interaction of HIV -1 and human salivary mucins J Acquir Immune Defic Syndr 19 94, 7:995 -10 02 Page of 10 (page number not for citation purposes) Virology Journal 2008, 5:59 10 11 12 13 14 15 16 17 18 19 ... aggregation of the virus by the mucins, leaving no free viruses to pass through the filter paper into the filtrate to cause viral infection On the other hand the 10 0% infection of the CEM-SS cells ... or compare the efficiency of HIV -1 aggregation by the crude pregnancy plug mucus and purified pregnancy plug mucins, at the end of the incubation period (1 h), the mixtures of (HIV -1 plus crude...
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báo cáo hóa học:" Inhibition of human immunodeficiency virus type-1 (HIV-1) glycoprotein-mediated cell-cell fusion by immunor (IM28)" docx
... Sodroski J, Springer TA: The lymphocyte chemoattractant SDF -1 is a lig- Page of (page number not for citation purposes) Virology Journal 2005, 2:9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 and ... 2.5 15 IM28 (ug/ml) Figure IM28 on fusion of TF228 .1. 16 cells with SupT1 cells Effect of3 Effect of IM28 on fusion of TF228 .1. 16 cells with SupT1 cells TF228 .1. 16 cells were mixed with SupT 11 cells ... HIV -1 infection, and SupT1 cells [15 ,16 ] The fusion of 293/CD4+ cells with TF228 .1. 16 cells was completely inhibited by a lower dose of IM28 than was the fusion of SupT1 cells with TF228 .1. 16...
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Báo cáo y học: "Inhibition of human immunodeficiency virus type-1 by cdk inhibitors" pdf
... instructions Page 13 of 14 10 11 12 13 14 15 Acknowledgements We would like to thank the members of the Kashanchi lab for experiments and assistance with the manuscript This work was partly funded by AI043894, ... mammalian RNA polymerase II carboxyl-terminal domain Proc Natl Acad Sci USA 19 93, 90 :11 167 -11 1 71 Zhang J, Corden JL: Phosphorylation causes a conformational change in the carboxyl-terminal domain of the ... Selectivity Name ACH2 J1 .1 OM10 .1 U1 CEM Infected Jurkat U937 Uninfected Alsterpaullone (10 μM) 11 25 15 37 89 92 88 Indirubin-3’-monoxime Indirubin-3’-monoxime-5’-indo (10 μM) (10 μM) 22 24 32 35...
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Báo cáo y học: "Autoprocessing of human immunodeficiency virus type 1 protease miniprecursor fusions in mammalian cells" pptx
... AH: Ordered processing of the human immunodeficiency virus type GagPol precursor is influenced by the context of the embedded viral protease J Virol 2005, 79 (16 ) :10 6 01- 10607 Pettit SC, Everitt ... E, Carter C: Deletion of sequences upstream of the proteinase improves the proteolytic processing of human immunodeficiency virus type Proc Natl Acad Sci USA 19 91, 88 (11 ):4776-4780 30 Mizushima ... inhibition of assembly and budding of virus- like particles Virology 19 93, 19 3(2):6 61- 6 71 16 Krausslich HG: Human immunodeficiency virus proteinase dimer as component of the viral polyprotein prevents...
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Báo cáo y học: "Phenotypic and genotypic characterization of Human Immunodeficiency Virus type 1 CRF07_BC strains circulating in the Xinjiang Province of China" pot
... XJDC19 81 SHXDC148 SHXDC168 SHXDC162 2 010 0 311 202 011 88 2 010 1324 2 010 1 810 2 010 0374 2 010 1796 2 010 014 1 2 010 0 419 20200407 20200084 2 010 0687 20200068 20200092 20200259 20200079 2020 010 8 2 010 0096 Tro- V3 sequence* ... FPD 3 41 590 2 91 322 463 298 588 376 75 237 319 15 5 30 11 484 61 128 19 6 48 12 5 16 57 41 139 264 387 365 479 2 21 246 14 1
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Báo cáo y học: "Characterization of the invariable residue 51 mutations of human immunodeficiency virus type 1 capsid protein on in vitro CA assembly and infectivity" docx
... (B), D51Q (C) and the wild type CAp24 (D) Bars indicate 10 0 nm tion of the D51E mutant was reduced by 2- to 6-fold as compared to the wild type The effect of the three CAp24 mutations on virus ... digestion The resulting plasmid was designated pET11a-CA and verified by sequencing The three HIV -1 CAp24 mutants, D51N, D51E, and D51Q, in the pET11a-CA vector were then engineered by site-directed ... Productive Human Immunodeficiency Virus Type Assembly Takes Place at the Plasma Membrane 2007, 81( 14):7476-7490 Kaplan AH, Manchester M, Swanstrom R: The activity of the protease of human immunodeficiency...
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Báo cáo y học: " Human Immunodeficiency Virus Type 1 Nef protein modulates the lipid composition of virions and host cell membrane microdomains" pot
... 2000, 1( 1): 31- 39 Gaus K, Chklovskaia E, Fazekas de St Groth B, Jessup W, Harder T: Condensation of the plasma membrane at the site of T lymphocyte activation J Cell Biol 2005, 17 1 (1) :12 1 -13 1 Gaus ... phosphorylation-independent downregulation of cell-surface CD4 by nef Nature 19 91, 350(6 318 ):508- 511 Michel N, Allespach I, Venzke S, Fackler OT, Keppler OT: The Nef protein of human immunodeficiency virus establishes ... Sci U S A 19 99, 96 (14 ): 816 7- 817 2 Thoulouze MI, Sol-Foulon N, Blanchet F, Dautry-Varsat A, Schwartz O, Alcover A: Human immunodeficiency virus type- 1 infection impairs the formation of the immunological...
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Báo cáo y học: "Requirements for the selective degradation of CD4 receptor molecules by the human immunodeficiency virus type 1 Vpu protein in the endoplasmic reticulum" doc
... 2003, 10 0 :15 154 -15 159 Schubert U, Clouse KA, Strebel K: Augmentation of virus secretion by the human immunodeficiency virus type Vpu protein is cell type independent and occurs in cultured human ... role of human immunodeficiency virus type vpu Proc Natl Acad Sci USA 19 89, 86: 516 3- 516 7 Klimkait T, Strebel K, Hoggan MD, Martin MA, Orenstein JM: The human immunodeficiency virus type 1- specific ... into human immunodeficiency virus type viral particles J Virol 19 94, 68 :19 26 -19 34 Ward CL, Omura S, Kopito RR: Degradation of CFTR by the ubiquitin-proteasome pathway Cell 19 95, 83 :12 1 -12 7 Tiganos...
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Báo cáo y học: " The predominance of Human Immunodeficiency Virus type 1 (HIV-1) circulating recombinant form 02 (CRF02_AG) in West Central Africa may be related to its replicative fitness" pps
... 5.59 4.93 4.52 446 4.20 CM CM CM 10 5 91 23 5 .18 5.83 5.38 Average MP8 01 MP582 MP1370 MP1033 MP1 416 MP1433 MP 812 AG AG AG AG AG 19 97 19 96 19 97 19 97 19 97 73 5.46 other4 AG AG AG AG AG Coreceptor ... A5 G AG7 AG7 AG8 G8 A7 G8 G7 A8 G7 19 97 19 97 19 97 19 96 19 99 19 97 19 97 19 99 19 98 19 99 19 99 19 97 19 99 19 99 19 99 R5 R5X4 X4 X4 R5 R5 R5 R5 R5 R5 R5 X4 R5 R5 R5X4 1Complete env nucleotide sequence ... http://www.retrovirology.com/content/3 /1/ 40 17 References 10 11 12 13 14 15 16 Robertson DL, Sharp PM, McCutchan FE, Hahn BH: Recombination in HIV -1 Nature 19 95, 374 :12 4 -12 6 Gao F, Robertson DL, Morrison...
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Báo cáo y học: " Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import" pps
... involvement of human immu- 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 nodeficiency virus type integrase in nuclear import of viral cDNA during acute infection J Virol 2004, 78 :11 563 -11 573 Nakamura ... following the first PCR, a second PCR was carried-out to amplify a portion of the HIV -1 LTR sequence from the first Alu-LTR PCR- amplified products The first PCR was carried out by Page 13 of 15 (page ... confirmed by Petit and colleagues by studying the intracellular localization of HIV -1 Flag-IN [18 ], other studies, using GFP-IN fusion protein, did not reveal the importance of K186Q and Q 214 / 216 L...
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Báo cáo sinh học: " Oligomerization of the human immunodeficiency virus type 1 (HIV-1) Vpu protein – a genetic, biochemical and biophysical analysis" ppt
... enhancement of particle budding: role of the cytoplasmic domain J Virol 19 96, 70:2669-2673 Page 10 of 11 (page number not for citation purposes) Virology Journal 2007, 4: 81 10 11 12 13 14 15 16 17 18 19 ... 20 01: 19 71- 20 41 Strebel K, Klimkait T, Martin MA: A novel gene of HIV -1, vpu, and its 16 -kilodalton product Science 19 88, 2 41: 12 21- 1223 Bour S, Strebel K: The human immunodeficiency virus (HIV) type ... recruits other proteins such as Skp1, Cul -1 and the Cdc34 E2 ubiquitin ligase [11 ] This results in ubiquitination of CD4, its dislocation from the ER and degradation by the proteosome [12 ,13 ] The stable...
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Báo cáo y học: "Near full-length genome analysis of low prevalent human immunodeficiency virus type 1 subclade F1 in São Paulo, Brazil" docx
... F1.RO .19 94.BCI2 F1.CD .19 85.85CD2 41 F1.ES.-.X1670 F1.RO .19 96.BCI12 F1.RO .19 94.BCI1 82 F1.RO .19 96.BCI15 F1.RO .19 96.BCI16 86 F1.RO .19 96.BCI19 79 F1.ES.-.P 114 6 F1.AO.20 01. HDC240 F1.AO.20 01. 01AOSNS48 F1.CD .19 97.KP35 ... BF1.BR.2002.02BR034 F1.CD .19 97.KTB136 F1.CD .19 98.patie nt 98CG869 10 0 F1.BE .19 94 .14 /00-37 F1.BE .19 95 .14 /00-33 F1.RO .19 96.BCI13 F1.RO .19 96.BCI 11 77 84 F1.FR .19 95.BCB85 75 F1.AO.20 01. 01AOCSE126 F1.RO .19 96.BCI20 ... BF1.UY.2005.UY05 4752 F1.BR .19 96.96RJ039 72 F1.BR .19 96.96RJ036 92 BF1.BR .19 90.BZ162A BF1.AR.2002.02AR 115 455 84 BF1.ES.2002.X12 41 F1.BR .19 95.SP004 83 F1.BR .19 95.RJ 014 F1.BR .19 93.BR020 F1.BR .19 96.96RJ035...
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Báo cáo y học: " Genotyping of TRIM5 locus in northern pig-tailed macaques (Macaca leonina), a primate species susceptible to Human Immunodeficiency Virus type 1 infection" pps
... http://www.retrovirology.com/content/6 /1/ 58 A 62 524 -1 KMZ-2 -1 50 528 -1 83 87 015 -1 97203 -1 65 932 01- 1 992 01- 1 5 51- 1 64 KMZ-5 -1 KMZ -1- 1 KMZ-4 -1 B C KMZ-4 KMZ -1 992 01 524 KMZ-5 97203 56 932 01 M.nem 5 51 87 015 M.mul KMZ-2 ... 925, 10 26, 10 87, 213 4, 22 51, 2303, 2 415 , 2494, 2506 and 2529) are in the coding region (39%), and the others (site 16 9, 248, 252, 350, 364, 462, 12 62, 12 65, 14 40, 17 18, 17 19, 17 21, 17 23, 17 59, 17 65, ... (KSCX1-YW-R -15 , KSCX2-YW-R092), and Scientific and Technological projects of China (2008ZX100 010 02, 2008ZX100 01- 013 , 2008ZX10005-005) and Yunnan (2006PT08, 2007BC006) References 10 11 12 13 14 ...
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Báo cáo y học: "A single amino acid substitution of the human immunodeficiency virus type 1 capsid protein affects viral sensitivity to TRIM5α" potx
... http://www.retrovirology.com/content/7 /1/ 58 Page of 10 A 10 000 10 000 NL-vifS NL-G 116 EvifS 10 0 10 0 .1 1000 p24 ng/mL 10 0 p24 ng/mL 10 00 p24 ng/mL 10 00 10 000 NL-4/5SG 116 E6/7S vifS 10 0 .1 0. 01 10 0 .1 0. 01 100 0. 01 Days after infection ... http://www.retrovirology.com/content/7 /1/ 58 Page of 10 10 000 10 00 p24 ng/mL 10 0 10 0 .1 CEMss (Hu) 0. 01 15 10 Days after infection 20 25 10 000 10 00 p24 ng/mL 10 0 10 0 .1 MT4 (Hu) 0. 01 15 10 Days after infection ... 4/5SG 116 E6/7S 40 16 10 Viral dose p24 (ng) 10 0 ** 4/5 10 Viral dose p24 (ng) ** 10 0 6E 10 0 .1 0 .1 100 4/5S6/7S 50 12 0 4/5 S6 10 0 Hu TRIM5α CM TRIM5α 60 WT 10 0 10 Viral dose p24 (ng) B 10 G1 1 G 116 E % infection...
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Báo cáo y học: "Nef-mediated enhancement of cellular activation and human immunodeficiency virus type 1 replication in primary T cells" docx
... 10 15 20 25 5C 5C-3 5C-AxxA 12 15 5C 5C-3 5C-AxxA 300 240 0 18 10 00 800 42 18 0 12 0 60 12 15 18 21 200 5C 5C-3 5C-AxxA 400 14 600 28 p24 (ng/ml) SF SF2 Nef 70 12 15 18 21 6I -1 6I 56 300 240 12 15 ... 6I 56 300 240 12 15 18 400 6I -1 6I 18 0 12 0 60 80 0 12 15 18 21 160 14 240 28 6I -1 6I 320 42 10 15 20 25 12 15 18 0 12 15 Days Post-Activation Figure Nef-mediated enhancement of replication is highly ... http://www.retrovirology.com/content/8 /1/ 64 Page of 17 A C Vector 51. 8% CD8 T cells CD8 6I -1 65.3% 46.4% 12 86 7 61 CD3 6I 662 B CD4 T cells 57.4% 72 .1% 61. 6% 11 07 13 36 10 66 Nef zsGreen+ 51. 5% 60.8% 42.6% 874 10 99 715 Nef zsGreen-...
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