purification of the enzymes substrates and assay conditions
Tài liệu Conditions of the surface water and ground water resources in the rural area of the Mekong Delta, Vietnam pptx
... approx 1.5 m The study site Hoa An is located in an area where an interaction of the tidal regimes of the South Chinese Sea and of the Gulf of Thailand eliminate each other Here, the tidal amplitude ... from the irrigation ditches of the rice fields At both study sites the values for the pH, BOD, NH4 and phosphate are conform to the requirements of the Vietnamese standards for the use of surface ... Outlook Regarding the ground water and the surface water quality the two study sites Hoa An and An Binh are typical for the conditions of the rural area of the Mekong Delta At present, there are substantial...
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Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
... by the cleavage of the bond between 1¢ and 2¢ carbons of the substrate The epimerization product is generated by the re-formation of the C–C bond after the reorientation of GA, which exposes the ... addition of the enzyme, and the middle three spectra were obtained 18, 35, and 70 after the addition of the enzyme The top spectrum is that of DHMP for comparison Only the NMR signals of the 2¢ and 3¢ ... at the N-terminus of the enzyme The binding properties of the Histagged and untagged enzymes were essentially the same (data not shown), and the binding data for SaDHNA in Table are those of the...
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Tài liệu Báo cáo Y học: Evolution of the enzymes of the citric acid cycle and the glyoxylate cycle of higher plants pdf
... three enzymes have an E1, E2, E3 subunit structure, and that some of the subunits of PDH, OGDH and OADH are related The functional and evolutionary relationships between the subunits of these enzymes ... (PDH) complex, the tricarboxylic acid cycle, and the glyoxylate cycle by examining the individual phylogenies of the 21 subunits comprising the 14 enzymes of these pathways as they occur in eukaryotes, ... all of the enzymes of the pathway (Fig 4), it is clear that only about half of the enzymes of the tricarboxylic acid cycle, the major pathway of carbon metabolism in mitochondria of oxygen-respiring...
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Báo cáo Y học: Baboon cytochrome P450 17a-hydroxylase/17,20-lyase (CYP17) Characterization of the adrenal microsomal and cloned enzymes docx
... [3,4] The dual activity of CYP17 and the differential regulation thereof have been the subject of many studies It is apparent that the normal expression and dual hydroxylase and lyase activities of ... Research and Ethics committee of the two Universities and complied with the ÔPrinciples of Laboratory CareÕ and the ÔNIH Guide for the Care and Use of Laboratory AnimalsÕ 1996 Adrenal glands, allocated ... P450 (A) Before the addition and (B) after the addition of NADH to the reference cuvette The reduction in the peak at 425 nm after addition of NADH is indicative of the presence of cytochrome b5...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... voltage of 120 V Other conditions were the same as in Fig In order to evaluate the sensitivity of the CE-ES-MS approach, a series of purified A salmonicida LPS standards containing between 100 lg and ... stainless steel needle (27 g) was butted against the low dead volume tee and enabled the delivery of the sheath solution to the end of the capillary column The separations were obtained on 90-cm long ... amount of 2-amino-2deoxy-D-galactose was identified in the hydrolysis products of both carboxyl-reduced CPS and LPS [19], confirming the presence of GalNAcA in the native CPS and LPS This was further...
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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx
... purification, the presence of impurities could not be excluded Therefore, reproducible and reliable methods for separation and purification are required for the accurate characterization of these ... 1961), and therefore we assume that FMN is lost during the purification process To achieve better stability of the enzyme, FMN was added to the protein solution immediately The requirement of non-heme ... defined as the amount of enzyme that catalyzes the oxidation of μmol of substrate per minute Results Cloning, expression and purification of 2, 5-diketocamphane 1,2-monooxygenase The 2,5-diketocamphane...
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current conditions and assessment on the effects of the caring, supporting and treating activities for aids patients at adult outpatient clinics in 3 provinces of vietnam, 2009 - 2010
... and count the time for the next dosage (82.7%), 16.3% of them skip the missed dosage and take the next dosage as usual, 1.0% of them did not know what to when they missed one dosage Most of the ... cells > 500 account for the rate of 5,2% and 10,2% A number of the patients with the number of CD4 cells
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Báo cáo khoa học: "Comparative studies of the water relations and the hydraulic characteristics in Fraxinus excelsior, Acer pseudoplatanus and A. opalus trees under soil water contrasted conditions Damien Lemoinea, Jean-Paul Peltierb and Gérard" pdf
... through the leaf with a pressure difference of 0.1 MPa The water flow was determined by measuring the changes of the leaf weight when the flow became constant The specific conductibility of the leaf ... selected, one in the valley of the Drac river, the other in a mountain stand in the Alps The experiments were conducted on leaf petioles (full symbols) and branches (empty symbols) These data are ... critical points, ψcav and ψ100 which indicated the water potential values that induced the start of the embolism, and 100% of the maximal hydraulic conductivity, respectively ψcav and ψ100 were measured...
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The specificity of the scientific field and the social conditions (Pierre Bourdieu)
... functioning of the field and is the condition of its and this amounts to the same thing the question of functioning, or, the field’s degree of autonomy (in relation, first, to the social demands of the ... institutions and dispositions, of the sum of the previous strategies of that agent and his competitors, strategies which themselves depend on the structure of the field through the intermediary of the ... terms of the time devoted to research) and in their nature (and especially in the degree of risk involved) on the amount of actual and potential recognition-capital which they possess, and on their...
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Báo cáo y học: "Foramen Magnum Arachnoid Cyst Induces Compression of the Spinal Cord and Syringomyelia: Case Report and Literature Review"
... superficial sensation in the bilateral upper limbs and trunk above the umbilicus, muscle wasting of the bilateral thenar and upper limbs, grade III muscle power of the upper and lower limbs, reduced ... fossa and excision of the arachnoid cyst were then surgically performed A straight median incision was made on the skull via the posterior temporal route The occipital squama was cut off and the ... within the cyst The cyst tightly adhered to the cerebrum, pons and cervical spinal cord The compartments were then separated and the posterior wall of the cyst was excised to break down the cystic...
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A study of semantic and pragmatic features of the adjective warm and its vietnamese equivalents
... “Introduction” states the rationale of the study, Semantics can be simply defined as the study of meaning It defines the aims, objectives and the significance, presents the is central to the study of communication ... review of the research Chapter 3: “Methodology and Procedures” mentions the method and procedures of the research Chapter 4: “Findings and Discussions” is the main frame of need to understand semantics ... element of the meaning of a word; a description of the characteristic feature of what the word refers to E.g the sense of “grasshopper” is “an insect which can jump high the study, which describes and...
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Environmental barriers to trade of the united states and implications for vietnam
... ASEAN, Regional Intergration Process and Challenges, Hanoi 14 Damien J Neven (2000), Evaluating the effects of non-tariff barriers, University of Lausanne 15 The Australian APEC Study Centre-Monash ... trường với điều kiện chấp nhận tiêu chuẩn môi trường; hạn chế thương mại đặt theo quy tắc MEAs [15] 1.1.2.2 Phân loại Theo nghiên cứu Lê Hoàng Lan [10], có loại rào cản môi trường thường áp dụng, ... nhiều nước giới, có Việt Nam Dù kim ngạch xuất hàng Việt Nam sang thị trường Mỹ theo chiều mũi tên lên, song, theo cảnh báo chuyên gia, doanh nghiệp xuất sang Mỹ cần phải thận trọng với "chiêu...
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Tài liệu COMING OUT OF THE FOODSHED: CHANGE AND INNOVATION IN RURAL ALASKAN FOOD SYSTEMS pptx
... them I am committed to continuing this work, and to bringing the power of the researcher and the research institution into their hands for their direction, for only they know the meaningful and ... example; the third, from which the title of this thesis was taken, presents the process and outcomes of contemporary food system change for the Athabascan village of Minto, AK, as they “come out of their ... remains an excellent example of the “commensal” community, where people live and eat together in a manner that is respectful of each other, of the land and the environment, and built upon a moral economy...
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Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx
... concentration of DA and the y axis shows the DA uptake after 10 The y axis demonstrates specific uptake, defined as total uptake subtracted the uptake in the presence of the DAT inhibitor cocaine (200 lM) and ... hydroxyradicals in the presence of iron [3,4] The cytosolic DA represents an additional risk of oxidative stress, as DA can auto-oxidize at the neutral pH of the cytosol and thereby form toxic ... [19], and NMR spectroscopic data of p25a homologs from mice, Caenorhabditis elegans and humans [20–22] demonTable Determination of Vmax and Km for DAT, NET and SERT in the presence and absence of...
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Tài liệu THE HISTORY OF THE RISE, PROGRESS, AND ACCOMPLISHMENT OF THE ABOLITION OF THE SLAVE-TRADE pdf
... within the shores of a little island Most of the islands of a continent, and many of these of considerable population and extent, were filled with them And the continent itself, to which these ... that the chasing ship may be detained by endeavours to pick them up; the dying and the dead strew the deck; women giving birth to the fruit of the womb, amidst the corpses of their husbands and their ... resist the voice of the British people, when it spoke the accents of humanity and of justice The slaves would have met the dawn of the first of August, the day which all the motions in Parliament and...
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Tài liệu DIRECTIVE 2004/39/EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL ppt
... firm and the client that set out the rights and obligations of the parties, and the other terms on which the firm will provide services to the client The rights and duties of the parties to the ... companies within the meaning of the second paragraph of Article 58 of the Treaty, in respect of the formation of public limited liability companies and the maintenance and alteration of their capital, ... on other regulated markets, even without the consent of the issuer and in compliance with the relevant provisions of Directive 2003/71/EC of the European Parliament and of the Council of on the...
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Tài liệu Histology of the Blood Normal and Pathological pptx
... blood, which on the whole fulfil the requirements of preserving the form and colour of the red corpuscles, of preventing their fusing together, and of allowing them to settle rapidly Of the better ... and nature of these seats of election for special proteid and other poisons The researches of Metschnikoff, of Kanthack and Hardy, of Muir, of Buchanan, and others, are supplementary and complementary ... take the blood from the tip of the finger, and only in exceptional cases, e.g in oedema of the finger, are other places chosen, such as the lobule of the ear, or (in the case of children) the...
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Tài liệu Youth Safety on a Living Internet: Report of the Online Safety and Technology Working Group pptx
... business of educating And to that we need to understand the risks and the way youth actually use the Internet and the social media they access through computers, mobile phones, game consoles and other ... knowledge to the students who are “digital natives.” The problem is that “we really don’t live in the same worlds.… There’s a lack of understanding of the kinds of things that happen, the way and the ... We have to take the time to train the teachers, to train the educators and the administrators and the counselors and the professionals who are going to be working with the kids.” And it takes a...
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Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx
... 2, the hyperfine splitting constants of the 15N nucleus of the distal NO and of the 14N nucleus of the proximal His of the GmHO-1 complex are closer to those of the rHO-1 complex than to those of ... analyses of the molecular features of the heme–GmHO-1 complex and of the mechanism of heme degradation were performed, and the results were compared with those for the heme complexes of SynHO-1 and ... The spectrum of the extract is shown in Fig 8B, exhibiting peaks at 404, 534, 636 and 684 nm; the latter two differ from the 660 nm band of the protein complex (Fig 8A) When the solution of the...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25