proteomic methods for biomarker discovery in a rat model of alcohol steatosis
Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
... with an intra-peritoneal injection of 400 mg/kg chloral hydrate (Pharmaceutical Plant of Tiantan Hospital, Beijing, China) The rectal temperature was monitored and maintained at 37.5°C A scalp incision ... infarcted brain parenchyma of transplanted rats (A- iii and A- iv) A comparable extent of class II MHC was noted in ischemic rats irrespective of any therapy but unremarkable in normal rat (panel B, ... staining of Class II MHC was evident in the infarcted brain parenchyma (B-i), along the meninge (B-ii), areas near the ventricular lining and vascular wall near the hippocampus (B-iv) of transplanted...
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Báo cáo y học: "Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain"
... cDNA sequence of rat TLR4 (GenBank accession NM_019178) were: 5’-CUACCAACAGAGAGGAUAU-3” (siRNA1), 5’-GUCUCAGAUAUCUAGAUCU-3’ (siRNA2), 5’-GAGCCGGAAAGUUAUUGUG-3’ (siRNA3) All siRNAs were chemically ... stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) (37) MAPKs are important for intracellular signal transduction and play critical roles in regulating neural plasticity and inflammatory ... neuropathic pain In addition, we have also demonstrated that suppression of TLR4 with intrathecal siRNA delivery could alleviate pain responses in a rat CCI model, suggesting that siRNA targeting...
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báo cáo hóa học: " Selective COX-2 inhibition prevents progressive dopamine neuron degeneration in a rat model of Parkinson''''s disease" potx
... dynamic data were acquired at two different coronal brain levels for an hour After an additional hour of decay time 11C-CFT (2 – mCi iv.) was administered and data were acquired as above Calibration ... hemiparkinsonian rats Brain Res 2004, 996:237-245 Adams FS, LaRosa FG, Kumar S, Edwards-Prasad J, Kentroti S, Vernadakis A, Freed CR, Prasad KN: Characterization and transplantation of two neuronal ... by an i.p injection of sodium pentobarbital (100 mg/kg) and perfused intracardially with heparin saline (0.1% heparin in 0.9% saline; 100 ml /rat) followed by paraformaldehyde (4% in phosphate...
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báo cáo hóa học: " Inhibition of NF-κB activation by 5-lipoxygenase inhibitors protects brain against injury in a rat model of focal cerebral ischemia" docx
... Justicia C, Camins A, Planas AM: Activation of nuclear factor-kappaB in the rat brain after transient focal ischemia Brain Res Mol Brain Res 1999, 65:61-69 Zhang W, Potrovita I, Tarabin V, Herrmann ... Schwaninger M: NF-kappaB is activated and promotes cell death in focal cerebral ischemia Nat Med 1999, 5:554-559 Rothwarf DM, Karin M: The NF-kappa B activation pathway: a paradigm in information ... by a rectal probe and maintained at about 37 ± 0.5 °C by a homeothermic blanket control unit (Harvard Apparatus, Holliston, MA) Cranial temperature was measured by HSE Plugsys TAM-D (Harvard Apparatus)...
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báo cáo hóa học: " CD200-CD200R dysfunction exacerbates microglial activation and dopaminergic neurodegeneration in a rat model of Parkinson’s disease" pot
... were analyzed and each sample was analyzed in duplicate Statistical analysis Statistical analysis of the data was performed using GraphPad Prism version 5.00 for Windows (GraphPad Software, San ... were obtained from a rat brain atlas by Paxinos and Watson The injection was made at a rate of μl/ using a 10 μl Hamilton syringe with a 26-gauge needle At the end of each injection, the syringe ... Contralateral rotation measurements following administration of apomorphine in each experimental group are shown in bar graph at days and 21 days post-6-OHDA injection Data are presented as mean...
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Báo cáo khoa học: "Role of mucosal mast cells in visceral hypersensitivity in a rat model of irritable bowel syndrome" ppt
... Princeton Instrument, USA) Three sections per animal were examined and the number of MMC was counted in at least 10 randomly selected fields using an image analyzing software Loss of intracellular granules, ... La et al animals received saline instead of acetic acid Rats were left to recover from colitis for days, and used for experiments at days after induction of colitis Mucosal mast cells counting ... balloon was cm inside the rectum Rats were placed in a transparent cubicle (20 × × cm) on a mirror-based elevated platform and were allowed to recover and acclimate for a minimum of 30 before testing...
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Quantitative evaluation of motor function before and after engraftment of dopaminergic neurons in a rat model of Parkinson''''s disease ppt
... engrafting ES-derived dopaminergic neurons Max area The size of the print area at maximal contact of all four paws during floor contact was smaller in rats weeks after 6-OHDA-lesioning than in ... changes in rats after unilateral 6OHDA lesioning and dopaminergic neuron grafting Some of the dynamic and static parameters were altered in rats with 6-OHDA-induced lesions including paw contact ... evaluate gait changes before and after transplant of dopamine neurons derived from embryonic stem cells (ES cells) in a unilateral 6-OHDA rat model of PD Materials and methods Animals and housing...
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Báo cáo y học: "Gender-based reciprocal expression of transforming growth factor-β1 and the inducible nitric oxide synthase in a rat model of cyclophosphamide-induced cystitis" ppsx
... emerge as a central mediator of the resolution of inflammation and induction of healing and in the bladder Our results therefore argue in favor of evaluating urinary TGF-β1 in IC patients in order ... reported as pg/mg of creatinine Panel A Urine levels of TGF-β1 at baseline In the absence of CYP injection, male and female rats excreted very low amounts of TGF-β1 Total (empty black triangle) and active ... Figure Urine levels of active and latent/total TGF-β1 at baseline and after CYP Urine levels of active and latent/total TGF-β1 at baseline and after CYP Active and latent/total TGF-β1 values are reported...
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Báo cáo y học: " Ultrastructural changes of the intracellular surfactant pool in a rat model of lung transplantation-related events" pdf
... surfactant preparations via the trachea has no impact on the amount of the intracellular surfactant pool [39], recent data suggest that alterations of the intracellular surfactant can occur already ... barrier, indicating that inactivation of the intra-alveolar surfactant predates the formation of alveolar edema [11] Consequentially, the prophylactic administration of exogenous surfactant turned ... conditions, leading to an increase of cytoplasmatic Ca 2+ concentration [25] Taken together, an intra-cellular surfactant pool within the AE2 cells can be distinguished from an intra-alveolar surfactant...
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Báo cáo y học: "Kinematic and dynamic gait compensations in a rat model of lumbar radiculopathy and the effects of tumor necrosis factor-alpha antagonis" ppsx
... p38 MAP kinase mediates pain in a rat model of lumbar disc herniation and induces motor dysfunction in a rat model of lumbar spinal canal stenosis Spine (Phila Pa 1976) 2007, 32:159-167 Igarashi ... percentage stance times of and a gait symmetry variable of approximately 0.5 A shift in either of these variables would indicate a shift away from balanced, symmetric gait For the statistical analyses ... Kinematic and dynamic gait compensations in a rat model of lumbar radiculopathy and the effects of tumor necrosis factor-alpha antagonism Kyle D Allen1,2, Mohammed F Shamji1,3, Brian A Mata2,...
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Báo cáo y học: "Cellular infiltrates and injury evaluation in a rat model of warm pulmonary ischemia–reperfusion" pot
... identical pattern of alveolar oedema in a function of time by using a histological examination (H&E) and assessment by wet : dry ratio An important increase of alveolar oedema was observed after ... underwent intramuscular injection of tramadol for pain control To prevent cooling, rats were placed on a warm-water pad during the operation and under a heating light during both ischemia and reperfusion ... model of acute lung injury, which was defined by quantifying apoptosis and alveolar oedema R2 Materials and methods Animals Male inbred Wistar rats (mean weight 225 g), obtained from Iffa Credo...
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Báo cáo y học: "Effect of magnesium sulfate administration on blood–brain barrier in a rat model of intraperitoneal sepsis: a randomized controlled experimental study" ppt
... leads to the formation of brain edema in septic rats Magnesium administration attenuated the increased BBB permeability and caused a reduction in brain edema formation in our rat model of intraperitoneal ... infusion of bacteria in a chronic porcine model Anesthesiology 2000, 93:793-804 18 van den Brink WA, Marmarou A, Avezaat CJ: Brain edema in experimental closed head injury in the rat Acta Neurochir ... resulting clot was then incubated at room temperature for 30 before implantation into the abdominal cavity The Escherichia coli strain was isolated from an intraabdominal collection from a patient with...
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Gene expression profiling in a rat model of neurodegeneration
... Chen 1998) and a non-degradable analog of glutamate KA is 30 times more potent than glutamate as a neurotoxin and can bind to both AMPA and KA receptors (KARs) in the brain (Bleakman & Lodge ... response and inflammation were observed at a very early time point to as late as months after excitotoxin administration Within hours, activation of resident microglia (Akiyama et al 1994), a hallmark ... RNA quality was analyzed using an Agilent 2100 Bioanalyzer, and cRNA generated and labelled using the one-cycle target labeling method cRNA generated from each sample was hybridized to a single...
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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx
... Sequence coverage (%) Fb Pb Observed change RPLP2 Parathymosin eIF 5A isoform B L7 ⁄ L12, mitochondrial Peroxiredoxin Annexin A5 Annexin A2 Aldolase A Fascin Pyruvate kinase VDAC-2 Stathmin Ran1BP GSTp ... eukaryotic initiation factor 5A (eIF 5A) , parathymosin, L7 ⁄ L12, annexin A2 , annexin A5 , aldolase A, fascin and peroxyredoxin 1] displayed quantitative differences, regardless of whether or not a- synuclein ... Germany) at 25 V for h The membrane was incubated with mouse anti -a- synuclein (BD Transduction Laboratories, Franklin Lakes, NJ, USA), mouse antiHSP70 (Zymed Laboratories, San Francisco, CA, USA)...
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Báo cáo hóa học: " Research Article Adaptive Probabilistic Tracking Embedded in Smart Cameras for Distributed Surveillance in a 3D Model" doc
... localization visualization of all your buddies instead of just a binary online/away classification Additionally, an idea is that available navigation software could be used on top of this to allow ... person handover unit and to a log file that allows for debugging and playback of recorded data Additionally, raw camera images can be acquired from any camera node for the camera GUI 3.3.2 Camera GUI ... single PC Camera nodes 3.2 Besides the preferred realization as smart camera, our system also allows for using standard cameras in combination with a PC to form a camera node for easier migration...
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Báo cáo y học: "Protease inhibitor-induced nausea and vomiting is attenuated by a peripherally acting, opioid-receptor antagonist in a rat model" doc
... naloxone on kaolin intake induced by ritonavir in rats Effects of pretreatment with naloxone on kaolin intake induced by ritonavir in rats Ritonavir-induced increase in kaolin intake was attenuated with ... Takeda N, Hasegawa S, Morita M, Horii A, Uno A, Yamatodani A, Matsunaga T: Neuropharmacological mechanisms of emesis I Effects of antiemetic drugs on motion- and apomorphineinduced pica in rats ... the article, final approval of the article, and obtaining of funding CZW was responsible for the study design, collection and assembly of data, analysis and interpretation of the data, critical...
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Báo cáo y học: "Effect of Zofenopril on regeneration of sciatic nerve crush injury in a rat model" doc
... crush injury in peripheral nerve Acta Med Okayama 2005, 59:37-44 Ikeda T, Choi BH, Yee S, Murata Y, Quilligan EJ: Oxidative stress, brain white matter damage and intrauterine asphyxia in fetal lambs ... histomorphometry of myelinated nerve fibers Walking tract analysis Functional recovery was analyzed using a WTA, and quantified using the sciatic functional index (SFI) [18] Rats were tested at 14th and 42nd ... experimental studies The healing process after nerve injury is reduced mainly free oxygen radicals rather than inflammation and edema [2] Therefore, in recent years many researchers started to stand...
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báo cáo khoa học: " Comparison of Radioimmuno and Carbon Nanotube Field-Effect Transistor Assays for Measuring Insulin-Like Growth Factor-1 in a Preclinical Model of Human Breast Cancer" doc
... conditions in the University of Maryland, Baltimore animal facility and maintained in accordance with institutional guidelines approved by the University of Maryland, Baltimore Animal Care and Use ... this article as: Jones et al.: Comparison of Radioimmuno and Carbon Nanotube Field-Effect Transistor Assays for Measuring InsulinLike Growth Factor-1 in a Preclinical Model of Human Breast Cancer ... a pattern of progressive adenocarcinoma with similar genetic changes and pathophysiology as seen in human breast cancers associated with BRCA1-mutations [11,12] Additionally, as in human BRCA1-associated...
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Báo cáo y học: "Effects of monoclonal anti-PcrV antibody on Pseudomonas aeruginosa-induced acute lung injury in a rat model" ppt
... hour after the airspace instillation of P aeruginosa PA103 (5 × 107 CFU) Bacterial instillate preparation and administration The instillate consisted of 5% bovine serum albumin (BSA), mg of Evans ... development of infection Therefore, we investigated the protective properties of intratracheally administered Mab166 and its Fab fragments on acute lung injury in a rat model of P aeruginosa pneumonia Methods ... administration of Mab166 intratracheally protects against P aeruginosa-induced acute lung injury Next, we evaluated the therapeutic administration of antiPcrV IgG in our rat model In this series of the experiments,...
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Báo cáo y học: " Protective effects of hydrogen-rich saline on monocrotaline-induced pulmonary hypertension in a rat model" potx
... Ohsawa I, Shinmura K, Tamaki K, Kimura K, Endo J, Katayama T, Kawamura A, Kohsaka S, Makino S, Ohta S, Ogawa S, Fukuda K: Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ... of Shandong (Grant: Z2008C09) Author details Artherosclerosis Research Institute of Taishan Medical University, Taian 271000, P.R.China 2Province Key Laboratory of Oral and Maxillofacial, Head ... and a gradual increase in arterial pressure beginning approximately days after a single dose of MCT [18] In our study, the rat model mimics several aspects of both primary and secondary human...
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