proteomic characterization of mesenchymal stem cell like populations derived from various tissue types
Báo cáo y học: "proteomic characterization of non-small cell lung cancer in a comprehensive translational thoracic oncology database" docx
... unique from other biorepository systems because it is not merely a listing of available tissue samples but rather offers a glimpse into the proteomic and genomic characterization of these tissues ... reflecting the multiple types of tissue obtained for each patient Therefore, samples were grouped according to tissue source: tumor tissue, normal tissue, and metastatic tissue for each patient ... additional tissue, not essential for the diagnostic process was available, this tissue was banked When available, samples of both normal and tumor tissues were collected from each subject Pathology Tissue...
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báo cáo khoa học: "The research on the immuno-modulatory defect of Mesenchymal Stem Cell from Chronic Myeloid Leukemia patients" pdf
... inhibitory effect of CML -derived MSCs on cell cycle arrest was also impaired Impaired effects of MSCs on T cell activation MSCs from CML patients could significantly inhibit activation of T cells The ... adhesion of tumor cells to the extracellular matrix, the degradation of the extracellular matrix by the proteinase secreted by the tumor cells, and the transfer and proliferation of tumor cells[36] ... 10 of 10 initiating cells in chronic myeloid leukaemia Nature 2010, 463(7281):676-80 39 Zhao ZG, Li WM, Chen ZC, You Y, Zou P: Immunosuppressive properties of mesenchymal stem cells derived from...
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Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc
... Institute of Biology, University of Iceland for providing us with cultures of Vibrio strain PA44, Dr Hiroshi Matsuzawa, Department of Biotechnology, The University of Tokyo, ´ for samples of aqualysin ... the N-terminal of VPR as determined by amino acid sequence analysis [25] and the beginning of the C-terminal extension based on results from MALDI-TOF mass spectrometry Residues of the catalytic ... loops of the C- and N-termini of the protein, calcium is coordinated by the carboxylate oxygens of Asp260 and the backbone oxygen of Thr16, in addition to 4–5 water molecules [57,58] Comparison of...
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Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot
... accelerated degeneration of the treated IVD [18-24] An alternative cell source are adult stem cells, in particular, bone-marrow -derived mesenchymal stem cells (MSCs) The use of these cells would allow ... Figure Cell viability/metabolic activity of injected mesenchymal stem cells Photomicrographs representative of caspase immunopositivity and polyAcells mRNA staining in mesenchymal stem cells injected ... Intervertebral disc cell- mediated mesenchymal stem cell differentiation Stem Cells 2006, 24:707-716 27 Richardson SM, Hughes N, Hunt JA, Freemont AJ, Hoyland JA: Human mesenchymal stem cell differentiation...
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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt
... of mesenchymal stem cell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stem cells from human bone marrow Stem ... 11 of 13 Figure The expression profile of 3A6 immortalized hMSCs converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem ... co-cultured CD34+ hematopoietic stem cells with 3A6 cells, we noted the formation of cobblestone areas from hematopoietic cells that transmigrated beneath the layer of 3A6 cells (Figure 3C) Previously,...
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Báo cáo y học: "Mesenchymal stem cell transplantation ameliorates motor function deterioration of spinocerebellar ataxia by rescuing cerebellar Purkinje cell" ppsx
... Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Lee OK, Kuo TK, Chen WM, Lee KD, Hsieh SL, Chen TH: Isolation of multipotent mesenchymal stem cells from umbilical ... Seuwen K: Stem cell characteristics of human trabecular bone -derived cells Bone 2002, 30:699-704 De Bari C, Dell’Accio F, Tylzanowski P, Luyten FP: Multipotent mesenchymal stem cells from adult ... Effects of Umbilical Cord Blood -Derived Mesenchymal Stem Cell Transplantation in Experimental Lupus Nephritis Cell Transplant 2011, 20:245-257 32 Parekkadan B, Milwid JM: Mesenchymal stem cells...
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báo cáo khoa học: " The HPB-AML-I cell line possesses the properties of mesenchymal stem cells" ppt
... Regulation of stemness and stem cell niche of mesenchymal stem cells: implications in tumorigenesis and metastasis J Cell Physiol 2010, 222:268-277 Ohishi M, Schipani E: Bone marrow mesenchymal stem cells ... Identification of subpopulations in mesenchymal stem cell- like cultures from human umbilical cord Cell Commun Signal 2009, 7:6 24 Xu X, Qian H, Zhu W, Zhang X, Yan Y, Wang M, Xu W: Isolation of cancer stem ... composed of round-polygonal and spindle -like cells Unlike the round-polygonal cells, HPB-AML-I cells with the spindle -like morphology attached to plastic surfaces Since HPB-AML-I was established from...
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Characterization of the secretion of mesenchymal stem cells and its relevance to cardioprotection
... interventions28 Mesenchymal Stem Cells In The Treatment Of Acute Myocardial Infarction With the emergence of stem cells as potential regenerative medicine, attempts to use stem cells to reduce ... cardioprotective exosomes Introduction Mesenchymal stem cells (MSCs) derived from adult bone marrow have emerged as one of the most promising stem cell types for treating cardiovascular disease ... Mitchell, W., et al., 2007 Elucidating the secretion proteome of human embryonic stem cell- derived mesenchymal stem cells Mol Cell Proteomics 6, 1680–1689 Tang, Y.L., Zhao, Q., Qin, X., Shen,...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... of the S mansoni EST database with the complete sequence of Tc52 revealed a clone (EST AI975843) with around 25% sequence identity with the GST -like domain of the T cruzi protein A fragment of ... sites of the bacterial expression vector pQE30 (Qiagen), the cloned vector was transformed into BL21 [DE3] cells (Novagen, Milwaukee, WI, USA) Briefly, a seed culture of the transformed cells ... GSH, 50 mM phosphate buffer, 0.5 units of glutathione reductase and an aliquot of the protein solution The reaction was initiated by the addition of mM of HEDS at 30 °C and followed by 340 nm...
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Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish (Lepidosiren paradoxa) docx
... alignment of the amino-acid sequence of lungfish calhepatin with other members of the S100 family indicates that the number of amino-acid identities between calhepatin and other S100 proteins ranges from ... of calhepatin was aligned with those of the human form of each S100 protein, except for MRP126 and ictacalcin, which have only been isolated from chicken and catfish, respectively The number of ... all mean ± SD; n ¼ 3) Tissue expression of calhepatin Fluorescence titration The intrinsic emission spectrum of calhepatin and those of the protein with increasing amounts of Ca2+ are shown in...
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NGHIÊN CỨU SỬ DỤNG TẾ BÀO GỐC TRUNG MÔ (MESENCHYMAL STEM CELL - MSC) TRONG ĐIỀU TRỊ UNG THƯ ĐẠI - TRỰC TRÀNG TRÊN CHUỘT (SPRAGUE DAWLEY) ppt
... injected with mysenchymal stem cells at the doses of 107cells/ml is statistically less than that of the control group (p
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Báo cáo khoa học: Proteomic characterization of lipid raft proteins in amyotrophic lateral sclerosis mouse spinal cord pot
... Lipid raft proteomics of ALS 18 Fukada K, Zhang F, Vien A, Cashman NR & Zhu H (2004) Mitochondrial proteomic analysis of a cell line model of familial amyotrophic lateral sclerosis Mol Cell Proteomics ... LC–MS ⁄ MS analysis of tryptic peptides from band #6 of the G93A lipid rafts (C) Tandem MS ⁄ MS of the peptide with m ⁄ z 589.31 from (B) The complete series of y ions was detected from the collision-induced ... carried out a proteomic analysis to profile alterations of lipid raft-associated proteins in the spinal cord of the G93A SOD1 transgenic mouse model of ALS Alterations of a consortium of 67 lipid...
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... Influence of Pi starvation on growth and secreted AP activity of tomato suspension cells Tomato cells cultured for days in the absence of exogenous Pi had only approximately 40% of the fresh weight of ... 8-day-old +Pi cells (approximately 63 and 25 g of cells were obtained per 500 mL culture of 8-day-old +Pi and –Pi cells, respectively) Eight days following subculture of the tomato cells into –Pi ... PAGE analyses of purified SAP1 and SAP2 (A) Non-denaturing PAGE on a 10% (w/v) separating gel of AP from the media of tomato suspension cells Lanes and 2, respectively, contain lg of culture media...
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university of california press fundamentals of the stem cell debate the scientific religious ethical and political issues dec 2007
... characteristics of stem cells: (A), Symmetric cell division leads to self-renewal of stem cells; (B), Asymmetric cell division leads to replacement of the stem cell and production of a sister cell, exemplified ... many, if not all, tissues Bryant and Schwartz differentiate embryonic stem cells from adult stem cells and point out that many tissues undergo continual replacement from stem cells They note the ... discovery of human embryonic stem cells The potential of these cells to replace diseased or damaged cells in virtually every tissue of the body heralds the advent of an extraordinary new field of medicine...
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báo cáo hóa học:" Proteomic characterization of HIV-modulated membrane receptors, kinases and signaling proteins involved in novel angiogenic pathways" ppt
... virus-infected cells [48] Expression of TNR9 receptor facilitates clustering of T -cell receptors at the cell surface of HIV-infected cells This interaction is conducive to activation of protein kinases, ... to the cell surface of endothelial cells and activate them Prolonged activation of endothelial cells by MAPK results in dysregulation of cell adhesion molecules that influence migration of the ... production of angiogenic factors involved in wound healing, repair of blood vessels, development of embryonic tissues and maintenance of cell shape [131,140,142] The development of embryonic organ systems...
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Báo cáo sinh học: "Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction" ppt
... days, majority of isolated adipose -derived stem cells expressing CD29 and CD90 characteristic of mesenchymal stem cells (n = 3) Note the spindle-shaped morphological feature of the stem cells (Right ... including bone marrow -derived mesenchymal stem cells (BMDMSCs), hematopoietic stem/ progenitor cells, and cells of embryonic origins, the ethical issue regarding the source and safety of allo- and xeno-grafting ... Journal of Translational Medicine 2011, 9:51 http://www.translational-medicine.com/content/9/1/51 Page of 17 Figure Engraftment of adipose -derived mesenchymal stem cells (ADMSCs) in renal tissue...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... G, Chen SH, Pan PY: Development and function of myeloid -derived suppressor cells generated from mouse embryonic and hematopoietic stem cells Stem Cells 2010, 28:620-632 Poschke I, Mougiakakos ... Functional characterization of human Cd33+ And Cd11b+ myeloid -derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines ... that of a positive T cell proliferation control (T cells alone), an induction negative control (CD33+ cells from medium only cultures), and an induction positive control (CD33+ cells isolated from...
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báo cáo hóa học:" Targeting of mesenchymal stem cells to ovarian tumors via an artificial receptor" docx
... phenomenon of MSCs was confirmed in different experimental systems [2,12] Other cell types, such as umbilical cord matrix stem cells (UCMS) [14], neural stem cells [15,16] and endothelial progenitor cells ... delivery of CX3CL1 to multiple lung tumors by mesenchymal stem cells Stem Cells 2007, 25(7):1618-26 12 Komarova S, Kawakami Y, Stoff-Khalili MA, Curiel DT, Pereboeva L: Mesenchymal progenitor cells ... long-term survival, and safety of human adipose tissue -derived mesenchymal stem cells transplanted in nude mice by high sensitivity non-invasive bioluminescence imaging Stem Cells Dev 2008, 17(5):993-1003...
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