property that a system performs its intended function in an unim paired manner free from deliberate or accidental unauthorized manipulation of the system 62
... vitro translated 35 S-labelled DYS-1 The signal intensity ofthe autoradiogram was quantitated with a radiographic analyser (Biorad) MW, Molecular mass markers T, aliquot ofthein vitro translation ... expressing yeast strain was selected and transformed with plasmids AD -AN4 50, AD-2¢5, AD-5¢1, AD-5¢2B, AD-5¢5B, or AD-6¢4 Transformants were selected on SD media minus tryptophan and leucin Interactions ... binding of dystrobrevin to dystrophin and functional activity of dystrobrevin The C elegans model organism was particularly well suited for the latter part because transgenic animals can be quickly...
... this, Marchand et al reported thatthe actuarial freedom rate from major bleeding in patients with an implanted CEP valve was 86.6%±3.2% at 14 years [1] These data suggest thata bioprosthetic valve ... the risk of thromboembolism, and consequently of hemorrhage associated with the use of warfarin, in patients with a mechanical valve Cannegier et al reported thatthe risk of hemorrhage in patients ... revealed no cases that had undergone CEP valve implantation inthe mitral position at more than 70 years of age, with subsequent SVD and explantation ofthe valve The average life expectancy of...
... PPs by airflow and maintain them at a uniform, low temperature Fig 4f displays branching ofthe air containers from tracheae that can pump air in and out ofthe air sacs and thence to the more delicate ... lateral aspects, and also in Figure in sagittal and axial views Both plates evince the fact thatthe greater volume ofthe gaster is occupied by the air sacs and that these sacs are separated at the ... resolution ofthe axial images and 88 µm for thatofthe sagittal images Any organs or tissue elements whose size or typical pattern is smaller (such as small tracheae), will not appear inthe image, and...
... et al Critical Care 1998, 2:79 http://ccforum.com number of bacteria after hand-washing /the number of bacteria before hand-washing)] Values are calculated from raw data and expressed as mean ... methicillin-resistant Staphlococcus aureus IN s in vitro [6] Moreover the flow of water enhances the antiseptic effects of this system by washing away bacterial contamination and organic material, ... details Intensive Care Unit, Osaka University Hospital, Yamadaoka, Suita, Osaka 565, Japan 2Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita, Osaka 565, Japan Published:...
... oxyheme appeared at 540 and 579 nm Then, a broad band appeared at around 660 nm, and was maximal 9–12 after initiation ofthe reaction The spectral features ofthe final reaction mixture were analogous, ... ofthe Soret band After several minutes, a broad band appears at around 660–675 nm; this increases in intensity with time, indicating the formation of biliverdin In this case, neither bands of ... (Fig 3A) Here, anisotropy ofthe gxy component is apparently larger than thatof heme–rHO-1, as shown inthe partly expanded spectra (a- 1 in Fig 3), indicating that in- plane anisotropy of heme...
... in normal growth medium Abbreviations Vpr: viral protein R; ATR: ataxia telangiectasia-mutated and Rad3-related protein; DCAF: DDB1-Cul 4A- associated factor 1; DDB1 and DDB2: damaged DNA-binding ... components that are relevant to Vpr Affinity chromatography and mass spectrometry identify DCAF1 as a potential interactor of Vpr Inan effort to identify cellular proteins that may interact with ... types of inactive Vpr mutants The first category would include Vpr mutants that fail to bind to DCAF1 The second type of mutants would include those that retain the ability to bind to DCAF1 but are...
... in normal growth medium Abbreviations Vpr: viral protein R; ATR: ataxia telangiectasia-mutated and Rad3-related protein; DCAF: DDB1-Cul 4A- associated factor 1; DDB1 and DDB2: damaged DNA-binding ... components that are relevant to Vpr Affinity chromatography and mass spectrometry identify DCAF1 as a potential interactor of Vpr Inan effort to identify cellular proteins that may interact with ... types of inactive Vpr mutants The first category would include Vpr mutants that fail to bind to DCAF1 The second type of mutants would include those that retain the ability to bind to DCAF1 but are...
... statistical analysis and participated inthe writing of this paper GM: participated inthe organ extraction and performed histological cuts and histological analysis JEMC: Supervised and participated ... than thatof group I and group III, and there was a delay inthe recovery of normal thyroid functionin group II in comparison to both groups I and III that may be caused by the inflammation induced ... administration to the study rats BGC: carried out the DNA extraction NAC: carried out organ extraction and participated inthe DNA extraction and inthe real time PCR AD: performed the statistical...
... monkeys, indicating limited bacterial invasion into the liver, or complete clearance, by days after boost vaccination Our pilot results warrant the testing of attenuated Lm vectors as part ofan orally ... demonstrated that oral inoculation of live attenuated Lmdd and i.v D-ala administration was safe and well tolerated in rhesus macaques Liver toxicity secondary to bacterial invasion can be a serious ... by the American Foundation for AIDS Research (amfAR) Grant 02882-32-RGV, National Institutes of Health Grant AI054183 to R.M.R, National Institutes of Health Grant AI078779 to F.R.F and National...
... however, the patient developed severe pain and vesicles along the tracts of V2 and V3 (Figure 1) The pain was characteristically different fromthe original pain of neuralgia This pain was of constant ... in this case report Case presentation A 54-year-old Caucasian man with a classic long-standing, left-sided maxillary and mandibular (V2 and V3) division primary trigeminal neuralgia, refractory ... medical management, underwent a posterior fossa exploration aimed at performing a MVD via a standard retromastoid craniectomy The trigeminal nerve was identified and exposed fromits origin to its...
... and death Animal studies suggest that agents such as heparin [10] and lidocaine [15] attenuate the thrombo-inflammatory response ofthe endothelium to luminal air In conclusion, the removal of ... between the atmosphere and a noncollapsed vein and a pressure gradient favoring the passage of air into the circulation [8] The patient described inthe present report met these criteria by having a ... patent lumen from skin to the central vein formed by the internal jugular sheath and by developing a pressure gradient while taking a deep inspiration immediately before or after a Valsalva maneuver...
... and clear information about the care available to them, inclusive of foreseeable risk, and thatthe provision of such information and the patient’s understanding thereof is of greater importance ... specifically non-steroidal anti-inflammatory drugs (NSAIDS), aspirin and paracetamol, surgery and anaesthesia Going to the hairdresser, driving or crossing the road and the chances of being struck ... risk associated with cervical manipulation remains controversial and poorly implemented and largely stems froma fear on the part ofthe chiropractor that to reveal the risk may alarm the patient...
... data interpretation and manuscript evaluation Genotyping data acquisition was supervised by CL All authors read and approved the final manuscript Competing interests The authors declare that they ... set revealed five haplotypes (TAGA, TAGG, TGAG, GGAA and X for haplotypes other than these four) inthe 480 animals ofthe LW population used for QTL mapping; [andro] is the mean +/- SD of androstenone ... housekeeping gene, TOB2B (GGGATGTCTGAAGAAGTACGAAAC//CATTCCTACAAGCCATTCCTTACG) Data was analysed with ABI software to obtain Ct values (threshold cycle) Four points of dilutions ofa mix of cDNA were...
... NirF in some organisms might be periplasmic but with an N-terminal transmembrane helix anchoring the protein to the membrane However, our bioinformatics analysis ofthe N-terminal sequences for ... cellular location For all these reasons we wanted to develop anin vivo system to investigate its role by making use ofthe generation and characterization ofan unmarked deletion in nirF The unmarked ... for analysis Western blots to detect strep II tags were performed using an alkaline phosphatase conjugate of strep-tactin antibody (IBA, Gottingen, ¨ Germany) according to the manufacturer’s instructions...
... (Ser16Ala and Ser127Ala) and a double-mutant protein (Ser16AlaSer127Ala) In this article, we report thatthe replacement of both invariant serine residues (Ser16AlaSer127Ala double-mutant protein) ... Ser127Ala mutant proteins or 12.5 lm ofthe Ser16Ala and Ser16AlaSer127Ala mutant proteins) Chorismate formation was monitored at 275 nm under anaerobic conditions For determination ofthe decay rates ... phosphateleaving group to facilitate C–O bond breakage Results Expression and purification ofthe Ser16Ala and Ser127Ala single-mutant proteins and ofthe Ser16AlaSer127Ala double-mutant protein The mutant...
... duration of each stage is an indication ofits ecological significance for the species and provides a more thorough understanding ofits life cycle Another advantage of this general scale for maturity ... male cephalopods, stages IV and V are short and perhaps of little ecological importance since they have to accumulate a large quantity of spermatophores inthe Needham sac at stage VI The duration ... of any stage means either that animals cannot be found at this stage orthatthe stage is of short duration For instance, stage IV (physiological maturation) is important for octopus and cuttlefish...
... [25–27] Mammalian transferrin can also bind various trace metals, including manganese, copper, and zinc, although the affinity of transferrin for these trace metals is lower than that for iron ... the demand for zinc; the latter pathway appears to be more important in females than in males, for the transport of larger amounts of zinc After its release from MYP in nutritive phagocytes and ... weight))1 at stage 1, which was less than half ofthatin ovary at stage 1, and remained at a similar value to stage Testes at stage were not analyzed because ofa lack of samples We calculated the amount...
... [17,33] The fact that all lack a IIA domain may support the speculation that IIACrr serves as the corresponding phosphotransferase A fascinating issue to investigate is whether the mechanism of inducer ... DNA sequence data [25] DNA databank and protein databank searches were performed using the BLAST server ofthe National Center for Biotechnology Information at the National Institutes of Health ... increase of RU caused by buffer components and protein binding Removal of MalK by application of washing buffer revealed an RU-increase ofthe baseline (dotted line) indicating solely the binding of...
... can lead to ABC systems failure and that organizational culture is ‘‘important’’ in explaining such resistance Argyris and Kaplan (1994) identify organisational culture change strategies that ... (TOP) At the heart of this organisational drive, was an attempt to engender greater flexibility of operational practices and an enhanced outward management orientation Many managers across the organisation ... the structuring of technical information reports Engineering analyses were indicative ofthe systematic fusion of diagrammatic and quantitative data and the blending of theoretical concepts and...
... humans and animals after SCI [46,47] and with results from intracellular recordings from sacrocaudal motoneurons that show a sustained and exaggerated firing rate in animals with SCI [48] Minutes ... system and LabChart software (ADInstruments, Inc, CO, USA) were used to acquire and analyze the data Once a single motoneuron was isolated at the left and right side ofthe spinal cord, few antidromic ... remarkable enhancement ofthe excitability ofthe corticomotoneuronal pathway induced by unilateral application ofthe dCMS This enhancement was observed in control animals and in SCI animals that...