production of l amino acids using immobilized aminoacylase
Effects of novel purine analogs and the role of aromatic amino acids on MRP4 functions
... W G L P L Q L A F F L C P N L W A M P V Q I I A L F C P L P A T T P I L M V 228 457 454 443 310 443 414 451 254 L L W M E I G I S C L A G M L L W L N L G P S V L A G V F L W R E L G P S V L A ... and peripheral blood mononuclear cells (Cole et al., 1992) MRP1 is located in the basolateral membranes of polarized epithelial cells rather than apical membranes where P-gp, MRP2 are located MRP1 ... enterocytes of the proximal small intestine, and proximal renal tubular cells as well as in the brain and placenta (Kartenbeck et al., 1996; Paulusma et al., 1999) Therefore, it is functionally similar...
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Microbial Production of Amino Acids in Japan
... l- Glutamate l- Glutamine l- Histidine l- Isoleucine l- Leucine l- Lysine l- Methionine dl-Methionine l- Phenylalanine l- Proline l- Serine l- Threonine l- Tryptophan l- Tyronsine l- Valine l- Dihydroxyphenylalanine ... Methods employing wild strain bacteria (l- glutamic acid, l- alanine, l- valine production) Methods employing mutants (l- lysine, l- threonine, l- arginine, l- citrulline, l- ornithine, l- homoserine, l- trypophan, ... Japan Table Amino acid production in Japan and the world in 1996 Amino acid Method Japan Glycine l- Alanine dl-Alanine l- Aspertate l- Aspergine l- Arginine and analogs l- Cysteine and analogs l- Glutamate...
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Báo cáo vật lý: "Preliminary Studies for Production of Fatty Acids from Hydrolysis of Cooking Palm Oil Using C. rugosa Lipase" pot
... Typical Hydrolysis Profile Figure shows a typical hydrolysis profile of palm oil at temperature of 45ºC for an initial palm oil concentration of 0.1 g palm oil/ml iso-octane at agitation speed of ... Chemicals (UK) All chemicals are of analytical grade and used without further purification 2.2 Enzymatic Hydrolysis A stoppered 250 ml conical flask was initially filled with g of cooking palm oil ... degree of hydrolysis, X is calculated as below: X,% = (ml NaOH used) (molarity of NaOH) (average molecular weight of fatty acid) 10 (weight of sample) (1) The rate of hydrolysis, r was calculated...
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Production of hydrogen using composite membrane in PEM water electrolysis
... from top of the cell at respective electrodes Thus evolved water, gases are lifted by circulating water on both sides of the single cell and collected in vessels atop of the electrolyser 2.5 ... operation is collected in vessels provided at the top of the single cell electrolyser The theoretical yield of hydrogen is calculated using Faraday laws equation(Eq:2) as given below [20] W H2 ... of single cell PEM water electrolyser The PEM water electrolyser is fabricated with SS316 material of 10 cm2 single cell Current collectors used are perforated titanium sheets The thickness of...
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Biodegradable Polymers Composed of Naturally Occurring α-Amino Acids
... “tail-to-tail”), parallel, antiparallel, or mixed (Figure 5.2) These could be polymers of other classes – polyurethanes and polyureas along with the said polyamides To render the polymers easily cleavable ... substituent of hydrophobic amino acids like: L- alanine (R=CH 3), L- valine (R=CH(CH3)2), L- leucine (R=CH2CH(CH3)2), L- isoleucine (R= CH(CH3)CH2CH3), L phenylalanine (R=CH2C6H5), L and DL-methionine ... O2N Cl NO2 NO2 O2N Cl Cl Cl O F F N F F Cl Cl Cl NO2 F Cl etc O A is divalent radical like: α,ω-Alkylenedicarboxylic acids (CH2)y with y = 2, 4, 8, 10, 12 O O Bis-(succinic acid)-α,ω-alkylene...
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Tài liệu Báo cáo khoa học: The mechanism of a-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase doc
... reversible quinonoid formation for the reactions of TPL with L- phenylalanine, L- methionine, and their deuterated analogs Substrate Solvent KS (mM) kf (s)1) L- Phenylalanine [a-2H] -L- Phenylalanine L- Phenylalanine ... internal return of the proton is equal to 0.33 For the reaction of L- phenylalanine the observed value of the internal return coefficient (a ¼ 0294) is only slightly less Consequently, it is reasonable ... Experimental data; solid line, the experimental curve calculated using Eqn (6) and kinetic parameters from Table The experimental values are much higher than the calculated ones, and the initial rate of...
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Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt
... Alliance for Chemistry) References Walsh CT, Zydowsky LD & McKeon FD (1992) Cyclosporin A, the cyclophilin class of peptidylprolyl isomerases, and blockade of T cell signal transduction J Biol ... bacterial strains that produce an enzyme catalyzing the production of N-methyl-lphenylalanine from phenylpyruvate and methylamine in order to develop a new method for the industrial production of ... N-methyl groups of depsipeptides and pilins, those of free N-methyl amino acids are derived from methylamine The reaction catalyzed by N-methylalanine dehydrogenase resembles that of alanine...
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Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are influenced by the C-terminal 42 amino acids doc
... Michaelis–Menten equation using a Km of 460 lM, a Vmax of 2.52 nmol PiÆminÆmg)1 and an offset of 0.1 nmol PiÆminÆmg)1 for SUR2A, and a Km of 41 lM, a Vmax of 0.73nmol PiÆminÆmg)1 and an offset of ... tightly than SUR2A The Km values of all four isolated NBDs were significantly larger than that of SUR2B Inhibition of ATP hydrolysis by MgADP and beryllium fluoride MgADP inhibited ATP hydrolysis ... not block ATP hydrolysis by full-length ATPase activity of SUR2A and SUR2B SUR2A or SUR2B; similar results were found for SUR1 [9] A possible explanation is that the ADP affinity of the full-length...
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Báo cáo khoa học: Control of mammalian gene expression by amino acids, especially glutamine potx
... through cytosolic O-glycosylation of Sp1 in Caco-2 cells J Biol Chem 278, 52504–52510 166 Vij N & Zeitlin PL (2006) Regulation of the ClC-2 lung epithelial chloride channel by glycosylation of Sp1 Am ... parallel, glutamine could also stimulate specific cell differentiation as shown by microarray analysis in a pancreatic b-cell line revealing multiple gene changes with a particular stimulation of ... Brasse-Lagnel et al transcriptional regulation via its production from the intracellular metabolism of glutamine, as we [44] and others [45] have recently reported in intestinal cells Finally, Table...
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Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf
... (Table 2) Fig Inhibition of L1 and L2 by NAT (A) Double reciprocal plots of inhibition of L1 by lM (e), 11 lM (D), 22 lM (s), 44 lM ( ), 88 lM ( ) and 120 lM (+) NAT (B) Double reciprocal plots ... eluted after application of 00.1 M NaCl (dotted line) Elution of proteins and of LAAO were followed as stated earlier Indicated areas of L1 and L2 peaks were pooled Inset: 10% SDS PAGE proles ... plots intercepting to the left of the v axis On the other hand, reciprocal plots for mutually excluding binding of two inhibitors will be a set of parallel lines [28,43] All the data presented here...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... cells mL)1 Twelve hours postinduction, lactacystine (10 lM final concentration), N-benzyloxycarbonyl -L- leucinyl -L- leucinyl -L- norvalinal (Z-Leu-Leu-Norvalinal, Z-LLnV, MG115; 50 lM final concentration), ... or N-benzyloxycarbonyl -L- leucinyl -L- leucinyl -L- leucinal (Z-Leu-Leu-Leucinal, Z-LLnL, MG132; 50 lM final concentration) was added to the medium and incubation continued for additional 0, 4, or ... expression levels of the individual variants in stably transfected induced cells (lg per 105 cells) *To visualize and quantify the individual variants in one blot, different numbers of cells were loaded...
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Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt
... (composed of 17.5 lL of rabbit reticulocyte lysate, 0.5 lL of mM leucine- or methionine-free amino acid mixture, or mM complete amino acid mixture, 4.0 lL of [3H]leucine (5 lCi), [35S]methionine (1 lCi), ... was used to transfect each plate of COS-1 cells along with lL of LipofectAmine (2 mgÆmL)1; Gibco BRL) in mL of serum-free medium After incubation for h at 37 °C, the cells were re-fed with serum-containing ... (M)GKQNSKLRPEMLQDL (M)GKQNSKLRPEVMQDL (M)GKQNSKLRPEVLQDL (M)GKQNSKLRPEVLQDL (M)GKVLSKIFGNKEMRI be the Lys residue at position To test this possibility, the effect of a Lys residue at position on the amino...
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Báo cáo khoa học: Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin pptx
... between plasmin and antiplasmin Molecular modelling of the C-terminal portion of antiplasmin The amino- acid sequence of the C-terminal 40 residues in antiplasmin is GNKDFLQSLKGFPRGDKLFGPDLKL VPPMEEDYPQFGSPK-OH ... (3 · 106 mL)1, in a total volume of mL), using a concentration ratio between expression plasmid and selection plasmid of 19 : (19 lg expression plasmid and lg selection plasmid) The cells were ... antiplasmin in detail by constructing seven single-site mutants of antiplasmin Fig Computer model of the C-terminal 40 amino acids in antiplasmin Some of the residues are labelled to facilitate...
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Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx
... mutated plasmid was transformed into Epicurian Coli XL1Blue Supercompetent cells by heat pulse Bacterial cells were transferred to LB-ampicillin agar plates and single colonies were isolated and ... R., Booth, B.J., Bullis, B .L. , Singh, D.N & Fliegel, L (2001) Functional analysis of polar amino acids residues in membrane associated regions of the NHE1 isoform of the mammalian Na+/H+ exchanger ... unexplained [37] The clinical picture of nonfunctional NTCP carriers would differ from patients with cholestasis where blockade of bile acid secretion at the canalicula pole of hepatocytes leads...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
... USA) Cells were plated in 24-well plates at a density of · 104 cells per well, in a total volume of 0.5 mL Cells were transfected with MMTV-LUC reporter plasmid (50 ngÆwell)1) and pSVAR.DBD.LBD ... complex would also explain the stimulation of AR23FxxLF27/AR.LBD inter- action by substitution of Q24 and N25 by alanine residues Structural analyses of AR.LBD/AR16)36 complexes have to reveal ... system, deletion of AR3)36 (GalAD-AR.NTDD3–36) completely abolished the ligand-dependent functional N/C interaction (Fig 2A) Deletion of the N-terminal 13 amino acids (GalAD- AR.NTDD1-13) resulted...
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Báo cáo " Research, design and fabrication of a high-power combiner using Wilkinson bridge of L-band " pptx
... the smaller ones The basic modules were designed by using the microtrip technology [4], which are small and portable (figure 4a) After simulink modelling, the Wilkinson bridge was designed using ... amplifying coefficient even more We have also investigated the S11 factor of the power divider Wilkinson on network analyzer, the result was relatively similar to that of the simulink model Afterthat ... outputs of port and port 3, and the lossless divider is not matched at all ports, and the resistive power divider is lossy The Wilkinson power divider has all ports matched and has isolation between...
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Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
... chymase Dog a-chymase Asp ⁄ Glu Asp Glu > Leu Glu ⁄ Asp Glu ⁄ Asp > Gln > Ala Ser ⁄ Leu > Glu ⁄ Asp Lys His Ser Ala Lys Ala Arg Arg Arg Arg Arg Lys Lys Lys Lys Lys Lys Lys rMCP-1 (b-chymase) rMCP-4 ... as follows: aromatic amino acids (Phe, Tyr, and Trp); negatively charged amino acids (Asp and Glu); positively charged amino acids (Lys and Arg); small aliphatic amino acids (Gly and Ala); larger ... L G H G L E D E A G L F S L D E G T V N E D S L L L A S E Q S E S T H M C R H H P R L L F F F T W H E W R V L D E C D M S A V M A V S R K E G S G S L T L L W V F I L P A C L W R P L L A A L L...
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Báo cáo khoa học: Chromophore attachment in phycocyanin Functional amino acids of phycocyanobilin – a-phycocyanin lyase and evidence for chromophore binding doc
... Several residues have been identified that relate to the different functions However, a general catalytic model is still lacking Investigations of the new class of distantly related lyases recently ... 173–177 Supplementary material The following supplementary material is available online: Fig S1 Kinetics of inactivation of CpcE by CHD (A) and PGO (B) The insets show plots of log(1 ⁄ t0.5) of inactivation ... photochemistry of bili-pigments – bilirubin and biliverdin dimethylesters and related linear tetrapyrroles Angew Chem Int Ed Engl 22, 656–674 Berkelman T & Lagarias JC (1986) Visualization of bilin-linked...
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FEASIBILITY OF CONTIMUOUS MAIN FERMENTATION OF BEER USING IMMOBILIZED YEAST pot
... of alcohols in beer and it has an impact on the flavour of beer Of the higher alcohols 3-methyl butanol (isoamyl alcohol) and 2-methyl butanol (amyl alcohol) also have an impact on the flavour ... (living) cells is less frequent, if production of vinegar and wastewater treatment are not included On the other hand, higher cells, especially animal cells, need to be immobilized Animal cells ... acceptable and well-balanced flavour profile similar to that of the traditionally fermented product (Maeba et al 2000) 3.2.9 Semi-industrial main fermentation at Hartwall Plc, Finland Hartwall's...
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Báo cáo khoa học: "AN EXPERT SYSTEM FOR THE PRODUCTION OF PHONEME STRINGS FROM UNMARKED ENGLISH TEXT USING MACHINE-INDUCED RULES" pdf
... syllable in that word will consist solely of a consonant cluster Similarly, if the word ends in a vowel then the final syllable will consist of a vowel cluster alone In all other cases, a syllable ... Key-syllable (final syllable excluding suffixes) is "in" Left-syllable (left of key-syllable) is "eem" Prefix ("pre") overlaps left-syllable ("eem") since they share an "e" Proper stress placement ... key-syllable (in terms of the v-c map representation); left-syllable (in terms of the v-c map representation); suffix type (neutral, weak or strong); preflx/left-syllable overlap (true or false)...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25