production by chemiluminescence and pseudoperoxidase activity of methb
Transplantation of skeletal myoblast in ischemic heart disease 1
... Immunostaining and time observation of the infiltration of macrophages Figure 3.17 Immunostaining and time observation of the infiltration of CD8+ lymphocytes Figure 3.18 Immunostaining and time observation ... immunostaining and doubling time of rSkMs Figure 3.27 Desmin immunostaining and flow cytometry assay for the purity of rSkMs Figure 3.28 Time observation of the infiltration of macrophages, CD8+, and CD4+ ... ischemia, and non-tumorigenic nature The present study was to investigate the characteristics of human SkMs in vitro and in vivo, to investigate and compare immune responses, SkM survival profile, and...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... understanding of genetic mechanisms of endothelial development and functions However, further analyses of vascular phenotypes in vivo have been hampered by the complicated relationship between ECs and ... Phosphorylation of VEGFR-2 and mitogenactivated protein kinases (MAPKs), but not of VEGFR-3, was induced by VEGF-A, whereas phosphorylation of VEGFR-2, VEGFR-3 and MAPKs was induced by VEGF-C, as ... be introduced by lipofection and maintained for at least days after transfection as assessed by GFP expression (Fig 4E) These Fig Isolation and characterization of uterine BECs and LECs expressing...
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot
... Expression of MUC15 in human and bovine tissues and cell types MUC15 mRNA expression was examined by PCR screening of commercial multiple tissue cDNA panels and oligo(dT) primed cDNA libraries and by ... Switzerland), thermolysin, chymotrypsin or elastase (Sigma, St Louis, MO, USA)] and Cloning of the bovine MUC15 cDNA by PCR with degenerate primers Isolation of total RNA from the mammary gland of ... PCR screening of bovine and human cDNA libraries showed the presence of MUC15 mRNA in human breast, bovine mammary gland, bovine lymph nodes and lungs of both species (Table 1) Of the identified...
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cancer cell culture, methods and protocols
... enzyme cofactors, and attachment factors (9) The concentrations of individual components of serum will vary with the age and health status of the animals of origin and for this reason fetal and newborn ... characterize and authenticate the individuality and identity of a cell line include DNA fingerprinting/profiling and cytogenetic analysis and these are covered in detail in Chapters and Techniques ... Daniels, D W., and Flandermeyer, R R (1981) Cross-contamination of cells in culture Science 212, 446–452 Nelson-Rees, W A and Flandermeyer, R R (1977) Inter- and intraspecies contamination of human...
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cell and tissue culture
... reports and certificates of analysis lity organization chart and site plan ils of servicing and maintenance of equipment ies for health surveillance and training of staff y directors and a record of ... extensivelyfor the productionof a variety of therapeutic and prophylactic protein products including hormones, cytokines, enzymes, antibodies and vaccines They offer the advantage of reproducibility and convenience ... quality standard and select an agency accredited to that standard ~ Q R CELL, BANKS ~ K ~ ~ plan study of a ~ r i correction ting and Preparation and §tructure definition of project of SOPS Fumigation...
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introduction to cell and tissue culture
... 1968), and hepatocytes (Thompson et al., 1966) allowed the study not only of growth but of the response to hormones and other environmental factors and the production and secretion of hormones and ... Ferrer and Technical Instrument Company, and Brent Kolhede of Lab Equipment Company Page xi Contents Chapter 1: Introduction The History of Tissue and Organ Culture The Practice and Theory of Tissue ... including production of genetically altered cell lines through mutagenesis and cloning, direct comparison of cells from normal and transformed tissues, the study of cellular physiology and metabolism,...
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báo cáo hóa học:" Isolation and culture of fibroblasts from endoscopic duodenal biopsies of celiac patients" pdf
... small amount of a soft tissue and have an important bacterial contamination caused by the common intestinal flora, the manual management of the endoscope and endoscopic forceps, and their passage ... Laboratory of Clinical Informatics and Cardiovascular Imaging, University of Milan by a single experienced reader blinded to image sequence and assignment Analysis algorithms were developed as a set of ... positive for FSP and negative for cytokeratin 20 in all the cultured and examined cells (Figure 3) Discussion Q-banding analysis of FBs from CD and non-CD subjects demonstrated a normal and stable...
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báo cáo hóa học: "Fibrillar beta-amyloid peptide Aβ1–40 activates microglial proliferation via stimulating TNF-α release and H2O2 derived from NADPH oxidase: a cell culture study" doc
... fA Figure Production of hydrogen peroxide by isolated microglial cells in the presence of Aβ1–40 peptide Production of hydrogen peroxide by isolated microglial cells in the presence of Aβ1–40 ... prevented by apocynin and catalase This suggests that activation of NADPH oxidase is sufficient to induce microglial proliferation via H2O2 production, and that activation of the oxidase by fibrillar ... peroxide production by microglia in the presence and absence of fibrillar Aβ1–40 There was no detectable change in the rate of hydrogen peroxide production immediately after addition of the peptide...
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Báo cáo y học: " Comparative analysis of cell culture and prediction algorithms for phenotyping of genetically diverse HIV-1 strains from Cameroon" pps
... Larger studies and data sets are needed from these regions to further substantiate these findings and to understand the complexity of the emerging diversity of HIV in this region of high diversity ... viral sequences of the strains to identify genetic subtype Phylogenetic analysis of partial sequences of gp41, p17 and pol region revealed that 53% belonged to CRF02_AG, 6% subtype F2 and 41% were ... that majority of viruses were recombinants of CRF02_AG with gene segments of other HIV CRFs and subtypes suggesting that newly emerging HIV strains in Cameroon may be recombinants of the predominant...
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báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture" ppsx
... the conception and experimental design of the in vitro HIV-1 manipulation and infection assays, in the analysis and interpretation of the data, and in the writing and revision of this report ... of PVP-coated AgNPs (0.3 and 0.6 mg/mL) were only cytotoxic to a small population of cells, affecting the viability of 20-23% of the cells in the cervical explant, which correlates with low off-target ... Institute of Allergies and Infectious Diseases and the National Institute of Health and Collaborators: MT-2 (from Dr Douglas Richman), H9+ cells (from Dr Robert Gallo), HeLa-CD4-LTR-β-gal cells and...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
... μl of a solution consisting of 2% agarose in DMEM (without any Page of supplements) into 96-well plates After curing of the agarose solution to each well, 180 μl of cell suspension was added and ... replaced by equal volumes (160 μl) of control medium and control medium containing 100 nM dexamethasone, 50 μM ascorbic acid, and 10 mM b-glyerolphosphate (all from Sigma), respectivey Thus, half of ... medium was changed every day After 3, 7, 10, and 21 days one quarter of the cultivated wells with microspheres of the + and - DAG group was harvested and transferred into Petri dishes for a washing...
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Báo cáo khoa học: " Thermal stability and inactivation of hepatitis C virus grown in cell culture" ppsx
... intracellular HCVcc and those released into culture fluid could be inactivated by each of these detergents, regardless of the presence of human serum, indicating that components of culture medium, ... by the Major Science and Technology Special Project of China Eleventh Five-year Plan (2008ZX10002013), and the financial grants from the National Key Science and from the National Institute of ... environment, and that stability of HCVcc is inversely correlated with temperature Effect of heat treatment on HCVcc infectivity To evaluate the sensitivity of HCVcc to heat treatment, aliquots of HCVcc...
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Báo cáo y học: "Calcium deposition in osteoarthritic meniscus and meniscal cell culture" pps
... = clusters of small calcium deposits inside the meniscus and limited number of clusters of small-sized and medium-sized calcium deposits at the edges of the meniscus, and = clusters of small-sized ... about 65% of the sections among all of the sections of OA menisci The third pattern of calcium deposition was widespread clusters of medium-sized and large-sized calcium deposits This type of calcium ... meniscus and widespread clusters of medium-sized and large-sized calcium deposits at the edges of meniscus Cell preparation Meniscal cells were prepared from the middle part of the anterior portion of...
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Báo cáo y học: "Cell culture and passaging alters gene expression pattern and proliferation rate in rheumatoid arthritis synovial fibroblasts" pdf
... design, and performance; writing, design and structure of the paper BR performed part of the experiments AK was involved in the evaluation and interpretation of data SL was involved in evaluation and ... log-linear region and the noise band The CP determined for the respective genes were normalized to those of 18S RNA to compensate for variabilities in the amount of RNA and for exclusion of general ... proliferation rate (Table and Figure 5) Discussion T-cell independent pathways, such as upregulation of proto-oncogenes, production of growth factors and the release of matrix-degrading enzymes...
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Báo cáo y học: " Distinguishing between linear and exponential cell growth during the division cycle: Single-cell studies, cell-culture studies, and the object of cell-cycle research" ppsx
... sensitivity of the analysis on data that are limited by slight changes in focus and by limited resolution of the optics and of the measurements on projected photographic images This degree of variation ... measurements of thousands of individuals at particular ages The central line of the height pattern is determined from measurements of thousands of individuals It is possible, and even probable, ... uptake of molecules during the division cycle of bacteria [24] Figure Reanalysis of the data of Kubitschek [16] Reanalysis of the data of Kubitschek [16] At the left is the original data of Kubitschek...
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Cell isolation and expansion using dynabeads
... infusion of polyclonal bead-activated and expanded T cells after transplant resulted in the early recovery of both CD4+ and CD8+ T cells In the setting of CLL, following infusion of bead-activated and ... isolation of eukaryotic cells [37], and the use of pure cell populations has now reached the eld of therapy through the initiation of clinical trials Ex vivo isolation and expansion of cells have ... induced, thereby enhancing in vivo survival and homing potential [41, 46, 48] Both CD4+ and CD8+ T cells are expanded by the process, thereby preserving both cytolytic/killing and T helper functions...
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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka
... digested by µl of RNase-free DNase I at 37°C for 15 min, and then synthesized RNA was precipitated by 2.5 µl of M LiCl and 75 µl of cold 100% ethanol after the digestion was stopped by µl of 0.5 ... essential roles of Oct4 and Stat3 in maintenance of stem cell pluripotency in mammals, and the unique features of the medaka, it seems equally important to understand the roles of Oct4 and Stat3 in ... expression of 13 Chapter I Introduction oct4 is regulated by the changes of DNA methylation The statue of DNA methylation is related to the changes of the chromatin structure of the mouse oct4 gene and...
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Phytohormones on cell growth and taxol accumulation via cell culture of taxus wallichiana zucc
... evidence of its superior spectrum of activity In addition, Susan Horwitz and coworkers demonstrated the unique mechanism of action of taxol, namely, promotion of tubulin polymerization and stabilization ... expensive and difficult A more promising approach for the sustainable production of Taxol and related taxanes is provided by plant cell and tissue cultures (Gibson et al., 1995) The capacity of plant ... (Yukimune Y et al., 1996) and tropane alkaloid production by suspension cultures of Datura stramonium (Bellica R et al., 1993) Increasing the activity of metabolic pathways by elicitation, in conjunction...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... H and Kadowaki, K (1985a) Flocculant production by Paecilomyces sp Taxonomic studies and culture conditions for production Agric Biol Chem., Vol.49, No.11, 3151-3157 - 299 - Journal of Water and ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the...
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Personality Traits and Workplace Culture
... of attraction, of added value and information exchange that give substance and fuel to the relationship The optimum degree of cultural match depends on one's psychological profile and, ultimately, ... High scorers enjoy the company of others and tend to be comfortable in groups Low scorers have a greater need for privacy and tend to avoid large groups of people Activity Level: Active individuals ... psychological meaning of the term refers to the readiness to challenge authority, convention and traditional values Low scorers are called conservative and prefer security and stability brought by tradition...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng 9 tr 25