processes determination of the reaction mechanism and fitting of equilibrium and rate constants
Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... 20–564), and the model is composed of residues 25–540 The overall structure of the model of IPU and the mutated residues in this study are shown in Fig To elucidate the mechanism of the substrate ... reducing end side of these substrates (Fig 5B) The activities of IPU for 32 mM of IMTG, panose and MM were assayed, and mg of the enzyme liberated 18.5, 14.0 and 7.37 lmolÆ min)1 of glucose, respectively ... of Glc +2 of the a-(1fi4)-linked glucose units of pullulan (Fig 4A) IPU is an anomer-inverting enzyme Comparison of the active sites of the model of IPU and Dex49A The active site structures of...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... with the fact that the positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and ... with the sequences of 1CEV and 1RLA and pasted into the structural alignment The alignment was then corrected by hand Since the sequences of the two arginases and agmatinase greately differ in the ... indicating that the environment of tryptophan residues is essentially conserved in the mutant enzyme On the other hand, the absence of major differences in the CD spectra of the wild-type and D153N...
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Báo cáo Y học: Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase ppt
... with the addition of citrate, suggesting the transfer of the labeled phosphate from the enzyme to citrate (data not shown) The results support an ordered mechanism of the enzyme reaction where the ... velocity of the reaction, Vmax is the maximum velocity, [S] is the concentration of citrate, Ks is the dissociation constant of the enzyme-citrate (ES) complex, and a, b, and c are the interaction ... ATP-citrate lyase The results provide the rst insight on the reaction mechanism of an ATP-citrate lyase with a heteromeric structure, and display some interesting features of the enzyme The His273...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... inhibition of the binding of the superoxide substrate and the other to product inhibition by hydrogen peroxide There are two mechanisms proposed for the reaction cycle of Mn ⁄ Fe-SOD: the 5-6-5 mechanism ... Mn-reconstituted enzyme and an additional water oxygen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed an equatorial plane (Fig 3B) The metal ion and the addi˚ tional ... (3) and ribonuclease A (4) The estimated molecular mass of ApeSOD is 57.0 kDa (B) Sedimentation equilibrium distribution of ApeSOD The line reflects the best fit of the data and indicates that the...
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Báo cáo khoa học: Spectroscopic investigation of the reaction mechanism of CopB-B, the catalytic fragment from an archaeal thermophilic ATP-driven heavy metal transporter potx
... monocytogenes and Bacillus subtilis, the N P and A domains of Archaeoglobus fulgidus CopA and the N P domains of Sulfolobus solfataricus CopB [1721] In order to study the reaction mechanism of the ATPase, ... b For mant-ATP in the absence of competitor, the value for Kdiss is given GTP showed afnities in the same order of magnitude On the other hand, ADP, the product of the ATPase reaction, bound to ... precursor molecule The time course of the difference band corresponds to rates of and s)1 in the presence or absence of CopB-B, respectively, which demonstrates that the release of ATP is much faster...
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catalytic activity and reaction mechanism of au,tio2 for the reduction of nox by propene
... on the catalytic activity To investigate the reaction mechanism of the SCR by propene over Au/TiO2 To understand the mechanistic effect of the additional CeO2 and Mn2O3 This thesis consists of ... activated on the Au surface in CO oxidation reaction [18] On the other hand, the adsorption of oxygen can occur at the vacancy sites of TiO2 [13] The adsorption of C3H6 on TiO2 and on Au islands dispersed ... which then react with adsorbed NO3- to produce N2 and other products The kinetic data showed that the presence of Cu2+ ions promote the rate of actate formation, nitrate formation, nitrate reaction...
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Supported nanosized gold catalysi the influence of support morphology and reaction mechanism 2
... the catalytic reaction and may affect the activity of catalyst, the addition of capping agent and reduction agent and the followed washing procedure are able to remove almost of chlorine in the ... spectrum of the surface The mix of elemental and cluster ions in the spectrum can generate a rich store of information regarding the chemistry of the surface layer Hence static SIMS is a technique of ... surface (the IR energy not absorbed by the sample) The latter is the radiation penetrated into the sample and then emerged It is the diffuse reflected radiation that carries the information about the...
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Supported nanosized gold catalysi the influence of support morphology and reaction mechanism 3
... the catalytic reaction and may affect the activity of catalyst, the addition of capping agent and reduction agent and the followed washing procedure are able to remove almost of chlorine in the ... For the AuCH and AuCM samples the calcination temperature did not have very much influence over the CO conversion of both reactions as the AuCP and AuDP sample Similarly, the CO selectivity of ... clarify the influences induced by the thermal pretreatment on the support , gold nanoparticles and in turn the influence on the catalytic activity towards CO oxidation and PROX reaction XRD profiles...
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Supported nanosized gold catalysi the influence of support morphology and reaction mechanism 4
... Oxidation of Carbon Monoxide in the Presence of Hhydrogen (PROX) Figure 4.9 and Figure 4.10 show the CO conversion and the O2 selectivity as a function of reaction temperature over the Au/TiO2 (NT) and ... band of the Au/TiO2 catalysts However the CO+O2 adsorption 121 results in evident observation of Ti-O-H as well as the enhanced intensity of the 3300cm-1 band, indicating the participation of the ... assumption of non-competitive adsorption, i.e the adsorption of carbon monoxide was not affected by the presence of oxygen Though it is rather weak the IR band at 2141 cm-1 appears in the spectra, and...
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Supported nanosized gold catalysi the influence of support morphology and reaction mechanism 5
... also detected These bands are assigned as the C—H stretching vibration of HCOO- ion This species was formed by the CO and the surface OH group: CO + OH- → HCOO-.28 The reaction of HCOO + O2 can ... Other than the bands at 1033, 1125 and 1285 cm-1, the CuO (NP) sample also showed bands at 1431 and 1492 cm-1 The band at 1492 cm-1 is assigned to the νas(CO2) of carbonites.30,31 The CuO (NP) DRIFT ... investigate the effect of surface lattice oxygen and adsorbed oxygen species on the CO oxidation reaction TPR profiles of CuO (CB), CuO (NP), Au/CuO (CB) and Au/CuO (NP) are shown in Figure 5.5 All the...
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Supported nanosized gold catalysi the influence of support morphology and reaction mechanism 6
... activity The reaction mechanism derived from the in-situ DRIFT studies is basically the same as those proposed by G.C Bond and H.H Kung In summary, the study in this thesis indicated that the improved ... of a reaction mechanism similar to those proposed by G.C Bond and H.H Kung All these results provide deep insights on the gold catalysis and valuable guidance for future study of it In this thesis ... study of the CO oxidation over Au/CuO could detect the presence of various Au species as well as a variety of reaction intermediates including formate, carbonate, and hydrocarboxylate, allowing the...
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Supported nanosized gold catalysi the influence of support morphology and reaction mechanism 1
... those of copper or silver, and accounts for the ready formation of the Au- state The former effect obviously explains the predominance of the AuIII state, which has the 5d8 configuration The AuI ... with the support and therefore influenced by it, while at the same time the fraction of coordinatively unsaturated surface atoms also increases, and this changes the physical properties of the ... determining the structure and composition of the finished catalyst, and in the COPPT method the support is formed during the preparation Hematite is an easily reducible support, and hence is one of the...
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Tài liệu TREES OF THE NORTHERN UNITED STATES THEIR STUDY, DESCRIPTION AND DETERMINATION FOR THE USE OF SCHOOLS AND PRIVATE STUDENTS doc
... The last of the four kinds of parts is found in the center of the flower, and is called the pistil It is this part which forms the fruit and incloses the seed The stamens and the pistil are the ... etc The taste and odor of the bark are characteristics worthy of notice: the strong, fragrant odor of the Spice-bush; the fetid odor of the Papaw; the aromatic taste of the Sweet Birch; the bitter ... other time of the year The plan of branching, the position, number, size, form, color, and surface of buds, as well as the arrangement of the leaves within the bud and the peculiarities of the scales...
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Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
... involving the measurements of both equilibrium and kinetic constants of the physical steps by equilibrium and stopped-flow fluorimetric analysis and the determination of the rate constants of the chemical ... measured the rate constants for the binding of HPO, the oxidized form of HP These measured rate constants are reliable and accurate, because of (a) the high quality of the stopped-flow data as illustrated ... experiments We ran the forward reaction (the formation of HP) using both DHNP and DHMP as the substrates and the reverse reaction (the formation of DHNP and DHMP) with HP and GA For the forward reaction, ...
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Tài liệu Báo cáo khoa học: Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of specificity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates pdf
... crystallographic studies of LlPDH complexes with physiological ligands, the substrate, the product of the enzyme reaction, and also with the cofactor These represent the first structures of a bacterial ... forms the floor of the active site The C1 of 6PG is placed near to the cofactor-binding domain with the phosphate directed to the loop between a10–a11 and the tail domain of the partner subunit There ... ordered in the ternary complex with NADP+ On the other side, the adenine forms hydrophobic interactions with Ala78 and Ala79 The hydrogen bonding of the enzyme with the adenine part of the cofactor...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... demonstrate our strategy of the combined use of ES-MS and NMR for analysis of the core-branching pattern and full sequence assignment of four oligosaccharides isolated from human milk These are ... determinant As the C3a ion is at m/z 690, this excludes the possibility of the other Fuc being at the Gal next to the subterminal GlcNAc The position of the second Fuc 3-linked to the internal ... oligosaccharides The significant aspects of this strategy are the combined use of CID MS/MS of singly and doubly charged molecular ions as precursors to deduce the Ó FEBS 2004 Sequence determination of oligosaccharides...
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Tài liệu Báo cáo khoa học: Unraveling the catalytic mechanism of lactoperoxidase and myeloperoxidase A reflection on some controversial features Elena Ghibaudi and Enzo Laurenti docx
... in the presence of H2O2 alone, a non-physiological condition We believe that the absence of direct evidence of the radical in the presence of cosubstrates limits the validity of extrapolating the ... LPO and MPO They deal with the the major importance of catalase activity in MPO with respect to LPO, the different sensitivity of the enzymes to Cpd III inactivation and the redox potential of the ... oxidize them [19] On the other hand, this fact does not represent proof of the presence of one or two oxidizing equivalents on the decay product of Cpd I The EPR spectrum of a spin-trapped radical...
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Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... 36% of the interaction of the full peptide with the binding cavity of SM3 The sizes of the surfaces were determined using distances between ligand ˚ atoms and protein atoms of less than A These ... a distance of A or less to the ligand The C-terminal part of the peptide (RPAP) has close contacts to the next protein in the crystal cell and is therefore stabilized on the surface of SM3 It ... reference pairs The build-up of the trNOE between Asp2-b and b¢ is much faster than that of the other two reference points indicating differences of the rotational correlation times of these proton...
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Tài liệu Báo cáo Y học: Mutations in the docking site for cytochrome c on the Paracoccus heme aa3 oxidase Electron entry and kinetic phases of the reaction pptx
... hypothetical second site have been made here for the bacterial enzyme by stripping subunits III and IV off the native complex, and by further destroying a large part of the acidic lobe(s) of the ... of the turnover number The reaction of cytochrome c oxidase shows a strict dependence of the turnover number on ionic strength We measured the oxidation of 20 lM horse heart cytochrome c by the ... on (a) the presence of subunits III Ó FEBS 2002 and IV in the Paracoccus enzyme, and by inference on the presence of cytoplasmically coded subunits of the mitochondrial enzyme as well, and (b)...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25