polymerase chain reaction and dna sequence analysis of bacterial ribosomal rna genes
Báo cáo y học: " A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control" docx
... as: Meng and Li, A novel duplex real-time reverse transcriptase -polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control Virology Journal 2010, ... Concentrations of HCV RNA and armored RNA were indicated by FAM and Cy5 signals, respectively Meng and Li Virology Journal 2010, 7:117 http://www.virologyj.com/content/7/1/117 Page of 1.34%, while ... Second International Standard for HCV RNA (National Institute for Biological Standards and Control (NIBSC), code 96/798, UK) was used to determine the Page of limit of detection (LOD) of the duplex...
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Báo cáo y học: "Multiplex Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) for diagnosis of natural infection with canine distemper virus" potx
... panparamyxovirus reverse transcription polymerase chain reaction and sequence analysis for the detection of Canine distemper virus in a case of canine meningoencephalitis of unknown etiology J Vet Diagn ... first-strand cDNA, the RNA and primers were mixed in × reaction buffers, 0.1 M DDT, 0.5 mM of each deoxynucleotide, 200 U SuperScript III reverse transcriptase (Invitrogen) and 40 U RNase inhibitor ... conducted most of this work under supervision of W-L H and T-J C M-S L, CY W, and S-S C participated in clinical sample collection K-H L, F-Y L, and T-H H participated in the sequence analysis of H gene...
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Báo cáo khoa học: " Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo" ppsx
... distribution pattern and nosogenesis of GPV in vivo in goslings Results Concentration of standard pVP3 plasmid DNA The concentration of standard pVP3 plasmid DNA was μg/μL, and the A260/A280 (ratio) ... simultaneous detection and quantification of DNA It is useful for understanding the pathogenesis of the disease and the Previous studies have examined the distribution of GPV in infected Muscovy ... numbers of pVP3 plasmid DNA were 2.76 × 1011 copies/ μL Development and optimization of FQ-PCR and conventional PCR After the optimization of FQ-PCR, we selected the final concentrations of each...
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Báo cáo y học: " DNA microarray analysis of rheumatoid arthritis susceptibility genes identified by genome-wide association studies" docx
... the Ministry of Health, Labor and Welfare of Japan Author details Graduate School of Frontier Bioscience, Osaka University, 1-3 YamadaOka, Suita-City, Osaka 565-0871,Japan 2Laboratory of Immune ... geneexpression profiling consistent? Prostate cancer as an example Plos One 2009, 4:e6551 doi:10.1186/ar2937 Cite this article as: Sugino H, et al.: DNA microarray analysis of rheumatoid arthritis ... and Korean population Arthritis Rheum 2009, 60:364-371 10 Gorlov IP, Gallick GE, Gorlova OY, Amos C, Logothetis CJ: GWAS meets microarray: are the results of genome wide association studies and...
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Báo cáo sinh học: " Molecular cloning and partial sequence characterization of the duplicated amylase genes from Drosophila erecta" pps
... partly sequenced (Boer and Hickey, 1986), and the coding regions showed a high degree of sequence similarity This, in addition to the close linkage of the copies, raised the possibility of sequence ... presented in Fig (inserts of kb and 2.4 kb, respectively, for the proximal and the distal genes) These subclones were used for sequencing DNA sequences were obtained by the dideoxy chain termination ... allozymes and the 2-dimensional gel electrophoresis (Cariou, 1987), mitochondrial DNA (Solignac et at, 1986), ribosomal DNA and histone gene family organization (Coen et at, 1982), DNA sequence...
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Recombinant DNA I - Basics of molecular cloning Polymerase chain reaction cDNA clones and screening
... specific DNA segments • Polymerase Chain Reaction • Requires knowledge of the DNA sequence in the region of interest • As more sequence information becomes available, the uses of PCR expand • With ... Denature, anneal primers, and synthesize new DNA: 14 duplex molecules of desired product PCR: make large amounts of a particular sequence • The number of molecules of the DNA fragment between the ... amounts of DNA • Not limited by the distribution of restriction endonuclease cleavage sites Polymerase chain reaction, cycle Primer Primer Template Cycle 1 Denature Anneal primers Synthesize new DNA...
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Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
... -2, and -3 in the synovium synthase-1, 2, and -3 and (OA) levels of the messages (RA) Relative expressionand rheumatoid arthritis for hyaluronanof knees in 2, and -3 and hyaluronidase-1, -2, and ... HPLC analysis for the measurement of the average molecular weight of HMW hyaluronan Analysis of message expression by quantitative realtime RT-PCR Message expression in the synovium of knees and ... levels for three isoforms of HAS and hyaluronidase in knee synovium obtained from control donors and patients with OA or RA, by quantitative reverse transcriptase polymerase chain reaction (RT-PCR),...
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A COMPARATIVE STUDY OF THE DIAGNOSIS OF PULMONARY TUBERCULOSIS USING CONVENTIONAL TOOLS AND POLYMERASE CHAIN REACTION* pot
... single gold standard was available for comparison of the performance of the individual tests, an analysis of results was done using a variety of standards Efficiency of microscopy, culture and PCR ... diagnosis of tuberculosis by amplification of mycobacterial DNA in clinical samples Lancet 1989; 4:1069 –71 Claridge JE, Shawar R, Shinnick TM, Plikaytis BB Large scale use of polymerase chain reaction ... later determined on the basis of response to ATT and radiological profile after months of treatment initiation and hence not considered in the final tally Indian Journal of Tuberculosis 36 KAVITA...
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Báo cáo khoa học: "Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus" pps
... demrofrep erew noitceted tcudorp dna noitacifilpmA elcyc hcae rof ces/C 02 fo pmar eht htiw nim rof C 06 ta pets noisnetxe/gnilaenna dna ces rof C 49 ta pets noitarutaned fo selcyc 55 yb dewollof ... noissucsiD IPD dna IPD neewteb RCP-TR lanoitnevnoc yb detceted eb dluoc ANR lariv dna suriv evil htob ,revewoH IPD.3 dna IPD neewteb ANR lariv rof evitisop erew sgip dna ,IPD dna IPD neewteb ... ,seniccav dna stset citsongaid rof sdradnats fo launaM seitoozipE seD lanoitanretnI eciffO 17 -36 ,92 ,4002 seneG suriV sniarts aisAnaP rehto dna aeroK ni slamina morf sesuriv esaesid htuom -dna- toof...
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Báo cáo khoa học: "Simultaneous detection of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in swine intestinal specimens by multiplex polymerase chain reaction" ppsx
... morf AND ]81,3[ seuqinhcet dradnats yb demrofrep erew siralullecartni o o L rof snemiceps eussit fo snoitanimaxe lacigolohtapotsih dna ,eairetnesydoyh B dna pps allenomlaS fo noitacifitnedi dna ... ciretne rehto dna setalosi dleif gnidulcni pps aripsyhcarB 01 ,.pps allenomlaS 11 ,AND cimoneg siralullecartni L AND dna sniarts lairetcaB sdohteM dna slairetaM dohtem citsongaid dradnats rof tset ... ;M pps allenomlaS dna siralullecartni L fo noitceted rof RCP xelpitlum fo yticificepS giF AND fo gp 05 ;5 enaL ,AND fo gp 001 ;4 enaL ,AND fo gn ;3 enaL ,AND fo gn 01 ;2 enaL ,AND etalpmet fo gn...
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Báo cáo y học: " Diagnosis of systemic toxoplasmosis with HIV infection using DNA extracted from paraffinembedded tissue for polymerase chain reaction: a case report" pptx
... extraction buffer and incubated at 56°C for 12 hours The supernatant was then purified by phenol-chloroform extraction and ethanol precipitation, and was resuspended in 25 mL of DNase-free buffer ... paraffin-embedded tissue for polymerase chain reaction: a case report Journal of Medical Case Reports 2010 4:265 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online ... resonance imaging; PCR: polymerase chain reaction Consent Written informed consent was obtained from the patient for publication of this case report and any accompanying images A copy of the written consent...
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Báo cáo y học: "Diagnosis of systemic toxoplasmosis with HIV infection using DNA extracted from paraffinembedded tissue for polymerase chain reaction: a case report" ppt
... extraction buffer and incubated at 56°C for 12 hours The supernatant was then purified by phenol-chloroform extraction and ethanol precipitation, and was resuspended in 25 mL of DNase-free buffer ... paraffin-embedded tissue for polymerase chain reaction: a case report Journal of Medical Case Reports 2010 4:265 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online ... resonance imaging; PCR: polymerase chain reaction Consent Written informed consent was obtained from the patient for publication of this case report and any accompanying images A copy of the written consent...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... remainder of the reactions were made up by µl PCR Buffer (Gibco BRL, Grand Island, NY), 0.25 µg of each primer, mM MgC12 in each CD4 reaction and mM MgC12 for CD8 reactions, mM total dNTP's, and 0.5 ... antilog of x when y = will give the concentration of unknown amount of DNA – that of CD4, CD8 or GAPDH The success of the RNA extraction and reverse transcription is determined by quantification of ... The standard curve plots the log cDNA/competitor band intensity (as determined by analysis of PICT files with NIH image) on the x-axis versus the log of incremental dilutions of competitor DNA...
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Báo cáo y học: " “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies.”" pptx
... both pp65 antigen and HCMV DNA Of the 19 unrelated (table 2) donor-recipient pairs, all recipients were positive for the presence of both pp65 antigen and HCMV DNA the results of which were ranging ... the presence of HCMV DNA and low count of pp65 antigenemia does not always lead to development of disease process which was made evident from the study And also, the importance of giving preemptive ... antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies.” Virology Journal...
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Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... products were cloned and sequenced, and the sequences were aligned with the H genes of a number of CDV strains available in GenBank using the MegAlign function of the DNAStar software package Thirty ... availability of genomic sequences, molecular diagnostic methods for detection of viruses have significantly improved[15] Complementary DNA and RNA probes have been used to detect RNA and mRNA of the ... transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus Virology Journal 2010, 7:86 Page of ...
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Báo cáo khoa học: " An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction" pptx
... recorded 108 cases of DHF and cases of DSS, with a total of deaths Large outbreaks of dengue occurred in Barbados in 1995 and 1997 and were associated with circulation of serotype (1995) and serotype ... serotype of the virus However, single-step reverse transcriptase polymerase chain reaction (RT-PCR) detection and typing of dengue virus offers a sensitive, specific, reproducible and rapid alternative ... being dengue positive and selected for confirmation and serotyping by reverse transcriptase polymerase chain reaction In 2003, 45 specimens were analysed of which 14 were males and 31 were females...
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Báo cáo thú y: " DNA methylation patterns detected by the Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) technique among various cell types of bulls" pot
... Methylation of satellite sequences in mouse spermatogenic and somatic DNAs Nucleic Acids Res 1984, 12:2807-2822 Liu ZJ, Maekawa M: Polymerase chain reaction- based methods of DNA methylation analysis ... clear and reproducible marker bands were counted, and the comparison of bands between genomic and digested templates was done according to the appearance of the marker mentioned above Number and ... 2School of Chemistry and Molecular Bioscience, Faculty of Science, The University of Queensland, Brisbane, QLD 4072, Australia 3School of Animal Studies, Faculty of Natural Resources, Agriculture and...
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Báo cáo y học: "Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit" pdf
... bacteria and between the 18 S and 5.8 S ribosomal spacer in fungi and is often used to detect bacterial/ fungal genes [8,9] For bacterial/ fungal DNA identification after amplification, the DNA of each ... Amplification and detection For detection of Gram-positive and Gram-negative bacteria, and for detection of fungi, 50 μL of each DNA extract was used The LightCycler SeptiFast kit and LightCycler ... pathogens by DNA Detection Kit and blood culture analysis, and we analyzed the number of pathogens that could be detected when the results of both assay methods were combined Materials and methods...
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Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx
... R&R Antibacterial Antibacterial Antibacterial Antibacterial Antibacterial Antibacterial Antibacterial Antibacterial Unknown Antibacterial Antibacterial Antibacterial Antibacterial Antibacterial ... N-terminal sequences of all basic Y proteins (basic Y6 through Y9), and repeats of the di- and tri-peptide sequences QQ and QQQ were observed in the amino terminal sequences of basic QH proteins and ... 0.1 lg of poly(A)+ RNA was performed using SuperScript III RNase H– reverse transcriptase (Invitrogen) and random primers One two-hundredth of the resulting first strand cDNA and 10 pmol of each...
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Báo cáo khoa học: Nucleosome positioning in relation to nucleosome spacing and DNA sequence-specific binding of a protein doc
... footprinting of the chromatin assembled over d35 shows the increased nuclease sensitivity of the top strand of the DNA in the presence of R3 The primer hybridizes to the bottom strand 93 bp upstream of ... difference of bp from d30 disturbs the protection of the ends of the core DNA in d25 and d35 Thus, despite the clear R3 footprint on L1 and L2 in d35 (Fig 5D,F), and the probable presence of the ... (1984) The locus of sequencedirected and protein-induced DNA bending Nature 308, 509–513 Thomas GH & Elgin S (1988) Protein ⁄ DNA architecture of the DNase I hypersensitive region of the Drosophila...
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