... 428AAA) and ExoS(LD426–427AA) into target cells The cells were then harvested and the resultant lysate was separated on a SDS ⁄ PAGE followed by immunoblotting with anti-Ras and anti-pan-Erk antibodies ... recover from the initial infection (Fig 3A, lanes 4, and 8) Together withthe results from the GST-pull-down assay (Figs and 2) using ExoS mutants withthe same amino acid substitution, it is noticeable ... positions 424 and 427 not mimic phosphorylated serine motifs important for theinteraction between 14-3-3 and ExoS Therefore, this interaction is more complex and must occur in another way, whereby...
... of the most influenced residues duringthe titration of [15N]b-DG(654–750) with thioredoxin-a-DG(485–620) [15] Therefore, we decided to mutate it to an alanine, together with two other phenylalanines, ... (lane 2), or containing DG–EGFP (lane 3) and its mutants DGPhe554 fi Ala–EGFP (lane 4) and DGPhe692 fi Ala ⁄ Phe718 fi Ala–EGFP (lane 5) (A) a-DG bound to the plasma membrane was probed withthe ... between the two important phenylalanines Phe692 and Phe718, does not perturb theinteraction between a-DG and b-DG recombinant peptides, as can be deduced by comparing the values of the apparent...
... study theinteraction between the 4-kDa peptide and the 43-kDa protein We also investigated the binding mechanism of the 4-kDa peptide, by alanine-scanning mutagenesis The results indicate that the ... be the most important in binding to the 43-kDa protein Mutation of Val29 and Phe31 to alanine resulted in the 43-kDa protein withthe lowest affinity, and substitution of Ile25 and Ile33 with alanine ... the importance of these amino acids in theinteraction between the 4-kDa peptide and the 43-kDa protein The secondary structures of these two fragments, as revealed from NMR spectroscopy of the...
... cultivating the land, and discussing the weather and the crops; a merchant reporting prices-current and the rates of exchange, and directing the making up of invoices; a politician speculating upon the ... since the new-fangled counselors have taken their oaths The importance, of which they consider the meeting of the Congress, and the result thereof to the community withholds the arm of vengeance ... as they, i e., the meetings, are so managed Such is the bitterness and rancor, the malice and revenge, the pride and vanity, which prevail in these men And such minds are possessed of all the...
... cultivating the land, and discussing the weather and the crops; a merchant reporting prices-current and the rates of exchange, and directing the making up of invoices; a politician speculating upon the ... since the new-fangled counselors have taken their oaths The importance, of which they consider the meeting of the Congress, and the result thereof to the community withholds the arm of vengeance ... as they, i e., the meetings, are so managed Such is the bitterness and rancor, the malice and revenge, the pride and vanity, which prevail in these men And such minds are possessed of all the...
... immunoblotting withthe anti-(calponin h3) Ig (Fig 1A, lane 1, panel c) This band showed a faint intensity when probed with anti-phosphotyrosine Ig (Fig 1A, lane 1, panel b) The cells transfected withthe ... (Gibco) according to the protocol of the manufacturer The transfected cells were incubated for 36 h and, after the addition of mM Na vanadate, incubated for a further 30 They were then washed twice ... °C and 5% CO2 Cells were transfected withthe pSV vector containing the cDNA encoding Fyn kinase [23], or withthe empty pSV vector The transfection was conducted by using the lipofectamin system...
... FNRox and FNRsq and, although it is not an isosbestic point for FNRsq and FNRrd, the absorbance change associated withthe FNRsq fi FNRrd transition is negligible when compared with that due to the ... binding and ET between FNR and NADP+/H, while the nature of the charge and the conformation of the side-chain at position 139 of Anabaena FNR modulates the precise enzyme interactionwiththe protein ... all of the processes analysed with E139Q FNR have values intermediate of those described for the E139K mutant and the wild-type FNRs Thus, the kcat and K Fd steady-state parameters and the kobs...
... combination withthe statistics and typical ages of embedded clusters The whole list of clusters within the ATLASGAL range is given in Appendix B, together withan important fraction of the compiled ... removal, they could be disrupted by another mechanism: tidal shocks from the natal dense environment On the other hand, they claim that the protostellar population with lower densities can be affected ... to the high over-pressure of the warm ionized gas (Te 104 K) with respect to the cold neutral surrounding medium (T0 in the range [10, 100] K) The expansion velocity can exceed the (significantly...
... from the NHãããS H-bond Rather than the CS antibond, the SS s* orbital is the recipient of charge in the next minimum m, this time extracted from both the N lone pair and the CO p* orbital An Nlp ... by the CO p bond (2.75 kcal mol1) A methyl group on the NMA engages in a CHãããO H-bond with S, and there is another contribution involving charge transfer from the S lone pairs to the CO p* antibonding ... combination with it Other than the NHãããS H-bond occurring in the global minimum, the CHãããO H-bond is the only other that occurs on its own in any of the structures, thus allowing an assessment...
... convey the meaning So withthe significant line, the significant light and shade, the significant look up or down, and the significant gesture, with means technically of the simplest, and, be ... beside these the greatest names in Venetian art, the Vivarini, the Bellini, Giorgione, Titian, and Tintoret The difference is striking The significance of the Venetian names is exhausted with their ... so many of the fifteenth-century improvements, is not withthe artists of the Renaissance, but withthe story-tellers and costumed fairytale painters of the transition, with Spinello Aretino and...
... ∞)) and a does not vanish identically on any subinterval (H2): Theadvanced argument θ Î C((0, 1), (0, 1)) and t ≤ θ(t) ≤ 1, ∀t Î (0, 1) Let E = C[0, 1] be the Banach space endowed withthe sup-norm ... Ioannina, Ioannina 45110, Greece Authors’ contributions GW completed the main part of this paper, SKN and LZ corrected the main theorems and gave two examples All authors read and approved the ... equations withadvanced arguments Nonlinear Anal 2003, 53:495-505 Yan JR: Oscillation of first-order impulsive differential equations withadvanced argument Comput Math Appl 2001, 42:1353-1363 Yang...
... is off resonant withthe ‘gate’ field [22] Thus, the aforesaid storage protocol can be applied to thesystem comprising the nanotip, the nanowire, and the emitter In the second stage, the ‘signal’ ... the nanotip and nanowire are excited surface plasmons [22] In the stage of photon storage, the ‘gate’ photon propagating along the nanotip is on resonant withthe transition jgi ! je1 i and the ... (SP) in thesystem consists of an emitter and a nanotip The emitter is initially in the ground state jgi and the dipole moment p of the emitter is parallel to the axis of the nanotip Under the action...
... Russian language capability The second group had knowledge of the discipline, but not the Russian language The third group had the Russian language capability but no expertise in the substance And ... The Magnitude of the Translation Problem Our most immediate concern is withthe translation of the Russian scientific and technical press for the benefit of the American scientific ... discussion He examined the machine output sentence by sentence and compared the translation withthe original Russian text His findings were that there was effective meaning transfer We then undertook...
... data and model fitting (n = 3) where C2 is the protein in the bulk, C10 is the concentration inside the gel, S and V1 are, respectively, the surface and the volume of film, V2 is the volume of the ... partially supported by the NINIVE (Non Invasive Nanotransducer for In Vivo gene thErapy, STRP 033378) project, co-financed by the 6FP of the European Commission, and by the IIT (Italian Institute of ... bulk, h is the massive exchange coefficient, Cs0 is the protein concentration on the surface of the film and finally ks is the desorption rate constant Substituting known values and by fitting the experimental...
... benefit the company Copier technicians can be linked by walkie-talkie to help each other They can share knowledge with each other instantaneously by simply clicking the handset Newer techs can lean ... capital the organization attains its performance and thus we can see the benefits outlined in the box connected by the dotted lines By social capital, they refer to the theory that the knowledge and ... this is an example of an external CoP, the same thing can be done within an organization, keeping the benefits more centralized and thus providing the company with a competitive 22 edge Organizations...
... and helps the company manages business strategies Some questions were defined and need to be answered within this thesis: What are the goals and strategies of the company? What makes manage and ... time-lagged and aggregate to provide valuable management information Johnsson and Kaplan claim that the organizations’ efforts should be managed through systems other than the financial ones Important ... after the tenth step, BSC has been included in the routine part of the strategic managementsystemThe communication within the organization follows the different units in the business plan and...
... mixed withthe contaminated room air by the time it reaches the inhalation zone of the occupants As a result, contaminant concentration is usually the same in the whole space and occupants breathe ... is mainly the momentum of supply air, here the momentum of the supplied air is small and the buoyancy is the dominant force for creating the room air movement The buoyancy is caused by the presence ... distribution without, with unheated and with heated manikin at L/s and 23.5°C/23.5°C isothermal case 71 Figure 4.12c Comparison of centreline velocity distribution without, with unheated and with heated...
... enzyme variant with mm Mn2+ for 10 at 60 °C These results indicate the existence of high and low affinity bindings of manganese ions to arginase II and provide an explanation for the manganese stoichiometries ... agreement withthe inactive state of dialyzed species of the His120Asn variant, its manganese content was almost undetectable (< 0.1 Mn2+Æsubunit)1) On the other hand, the half active wild-type and ... convert the enzymes to their fully active state In contrast, the arginase activity of the Asn149Asp variant was practically undetectable, both before and after the incubation withthe manganese...
... constant, but varies with atrial contraction and instantaneous atrial volume) The pressure of the point at the bottom right corner of the loop is the pressure in the ventricle at the end of the ... mechanical properties by plotting the time course of change in the slope of the instantaneous PVR Above, we referred to the slope of the ESPVR as an elastance Similarly, we can refer to the slopes ... obtaining these loops the properties of the arterial system were changed (specifically, the total peripheral resistance was modified) without modifying anything about the way the ventricle works The...