... entire paw Therefore, the disease score for individual rats ranges between and 16 Adoptive transfer of arthritis Arthritis was transferred adoptively to naive 7-week-old syngeneic rats by intravenous ... (Figure 3a) Within these subsets, presumptive Mϕ were identified by expression of CD163 (Figure 3b) The numbers of MHC IIhiCD163+ cells increased progressively over the 14 days after adoptive transfer ... arthritis and to prepare cells from hind-paw SRT, groups of five rats were examined at days 3, 6, 9, 12, and 14 after adoptive transfer Five healthy rats were included as controls Isolation of cells...
... known to be more sensitive than the Affymetrix gene chip technology, semiquantitative RT-PCR was introduced to validate Affymetrix-derived mRNA expression levels in individual patient samples ... at the Department of Orthopaedic Surgery, University of Leipzig, Germany All samples were collected with the approval of the Ethics Board of the University of Leipzig Clinical, biologic and demographic ... were determined by semiquantitative reverse transcription polymerase chain reaction (PCR) The figure shows a representative analysis of eight cDNA samples derived from patients with RA and of...
... extremities, 27(74.1%) were STAT3 positive and 15(42.9%) were pSTAT3 positive 12 out of the 14 tumors in the retroperitoneum (85.7%) were STAT3 positive while pSTAT3 positives were (57.1%) Tumors in the ... also indicates that increased activation of STAT3 could be associated with more aggressive biological behavior of soft tissue tumors Although constitutive activation of STAT proteins is not the ... constitutively activated in rhabdomyosarcoma and osteosarcoma cells It has been previously reported that STAT3 is overexpressed in cutaneous angiosarcoma, pyogenic granuloma, Figure Representative...
... formation of compression wood in actively growing 3-year-old spruces by maintaining at a 45° angle (relative to vertical) (Fig 5) After 21 days of growth in this leaning position, characteristic ... R2R3-MYB proteins This neighbour-joining (1000 Bootstraps) tree was based on the Clustal W alignment of the complete coding sequences of 13 spruce and five pine MYB proteins identified in this study ... recent gene duplications with relatively low sequence divergence levels Our work provides a clear indication that the conifer MYB family structure is not all that divergent from that of the angiosperms,...
... corresponding mRNA [29] This result suggests a defective regulation of the hepatic 3β-HSD expression at the transcription level [29] Although 3β-HSD is active in both liver and testis, only expression ... androstenone on CYP2E1 activity, but with concentrations very different from those present in vivo Review of genetic aspects of boar taint 139 CONCLUSION AND PERSPECTIVES In this paper, we have discussed ... suggestive (SSC10, SSC11, SSC18) effect on androstenone level only at one stage i.e 100 or 120 days of age In slaughtered individuals, the only QTL detected were the QTL on SSC7 and the suggestive...
... Plasmids encoding constitutively active (CA) and dominant negative (DN) kinases and GTPases were use in this study These include CA-RhoA, CA-Cdc42, CA-Rac1 and their respective DN forms and the corresponding ... of Rac1 The relatively potent activation of the endogenous CTGF/CCN2 gene by the active mutants of RhoA and Cdc42 may simply reflect the ability of Rho GTPases when activated individually to recruit, ... relatively sustained increase of GTP-RhoA compared with the transient increase in GTP-Cdc42 and GTPRac1, the levels of which returned to those in control cells within 15 of stimulation This activation...
... human cord blood-derived mast cells [38], heparin-treated umbilical cord blood was obtained from normal full-term vaginal deliveries under auspices of the Kyungpook National University Hospital ... more than 50% of the native enzyme (Fig 1C) The distribution of the KE2 mutant between the two fractions was similar to that of the native enzyme (Fig 1C) In contrast, the activity of the KE2RS2 ... and -V) These enzymes are in common active on ÔrearrangedÕ cellular membranes that have been primed by various cell activators [6–12], yet sPLA2-IID, relative to -IIA and -V, shows apparent preference...
... increased glycolytic activity at constant activity of the mitochondrial respiratory chain would suggest an increased activity of ATP synthesis To examine whether this increased activity was reflected ... progressive neurodegenerative disorder, which is inherited in an autosomal dominant fashion [1] It affects approximately one in 10 000 individuals The disease is characterized by motor and cognitive ... semi-quantitatively less than 15% and 5% deviations, respectively The reasons why the simulations are not satisfactory for a given metabolite concentration in models 1–5 are shown qualitatively There...
... staged at embryonic day 18 (E18) delivered by cesarian section and newborn pups (1–6 h after spontaneous delivery) were used to study postnatal development of the liver Cell lines HEK-293T cells were ... in cells from the hematopoietic system Citrin is expressed at high levels in the liver This molecule is the liver-specific AGC isoform, as citrin deficiency causes Ó FEBS 2002 3318 A del Arco et ... does not compensate for the loss of citrin in liver Surprisingly, however, we detected a low but unequivocal presence of aralar1 protein in the adult liver (Fig 2B, see also Fig 3C) Furthermore,...
... more effective ovarian cancer treatments These molecular targets may also be especially important in treating triple negative (ER negative, HER2 negative, progesterone receptor negative) breast ... expression A similar observation was observed in MCF7, ERα positive, ERβ negative versus MDA-MB-231 which is ERα negative, ERβ positive [10] ER was previously shown to downregulate the FN1 gene ... suggested that abnormal expression of STYK1 results in a constitutively active state caused by disruption of an inactive vs active state equilibrium However, we did not see an appreciable difference...
... presence of three fold coordinated negatively (K- center) and positively (K+ center) charged silicon atoms as traps for holes and electrons, respectively [48], and third to Si-Si bond or nitrogen vacancy ... sufficient to quench the luminescence of a Si-np [42] In this respect, the passivation of silicon- and nitrogendangling bonds acting as non-radiative recombination Figure Room temperature PL spectra ... N ) The PL peak intensity increases about 28% due to this additional forming gas annealing step, indicating passivation of some non-radiative recombination centers It is noteworthy that the overall...
... below the critical threshold of inactivation [39, 51] In addition to this “colligative” effect, a more specific “non-colligative” effect has been postulated This effect relies on interactions between ... the log values of relative conductivity (RC; %) of the incubation solution and the duration of bark tissue incubation (h) during the investigation period The slope is a relative measure of freezing ... crystallization in the vicinity of biomolecules [39] In this regard, di- and trisaccharides seem to be more effective than monosaccharides [11] This might explain the fact that, in the present investigation,...
... Example More generally, let W be any irreducible T -module We say W is thin if dim Ei∗ W for all i Suppose W is thin Then W is dual thin, i.e., dim Ei W ∗ for all i, and there are integers ǫ (endpoint), ... was then derived from the existence of a specific example We may remark that (6.3), (6.4) supersede these results and give an answer to the problem raised in [31, p 907, Remark] We end this section ... α, β) There are many results characterizing this property in terms of substructures of graphs; see e.g., [42, Theorem 7.2], [41] We show that a nontrivial descendent Y with width w is convex (i.e.,...
... often positive for rheumatoid factor (Table 2) Most of the patients in the cohort with erosive RA were receiving DMARDs, whereas only a minority in the group with nonerosive RA were receiving DMARDs ... with nonerosive RA (67%) than with erosive RA (43%, P = 0.023) (Fig 3) This difference was due in part to the high prevalence of TIMP-2 antibodies in the samples of patients with nonerosive RA (44% ... TIMP-4 Any TIMP-1 TIMP-2 TIMP-3 TIMP-4 Any Controls Erosive RA Non-erosive RA Antibodies against TIMPs in patients with erosive or nonerosive rheumatoid arthritis (RA) and in controls The presence...
... presentation of arthritogenic antigens to autoreactive T cells [19,33-35] This presentation may drive aberrant memory T cell responses, promoting B cell activation and immunoglobulin class switching ... Five distinct subsets of lineage-negative HLA-DR+ DCs have been classified in humans Four subsets – CD1b/c+, CD16+, blood dendritic cell antigen (BDCA)-3+ and CD34+ – are myeloid-lineage derived, ... to examine DC function ex vivo Future technical refinements will be required to perform the desired extensive in vitro analyses of DC function that will more comprehensively address their functional...
... fluid and bone marrow of patients with inflammatory and degenerative arthritis were reported to be negative for CD45 and to be positive for D7-FIB, CD13, CD105, CD55, and CD10 [13]; these mesenchymal ... (adhesion and holding beneath) In vivo, such thymic nurse cells were thought to support the development and expansion of thymocytes and to also play a role in positive/negative selection of T cells in ... and activation of lymphocytes and monocytes, and their unique capacity to promote the differentiation of osteoclasts from myeloid precursors in a receptor activator of NF-κB/receptor activator...