phương pháp ly trích bằng sóng siêu âm
Folk medicine cratoxylum cochinchinense antioxidant but cytotoxic
... signaling mediators, ROS are present ubiquitously within all cell types; they are easily synthesized, readily activated, highly diffusible, and are easily degraded when a mission has been fulfilled ... Sometimes + + + + Usually Sometimes + + + (85-kDa fragment) + (50- to 62-kDa fragments) + + + + + Sometimes Sometimes + Late Usually + + DEVD, Asp-Glu-Val-Asp; PARP, poly(ADP-ribose) polymerase Modified ... inactivation of DNA repair enzymes such as poly(ADPribose) polymerase (Van Rensburg et al., 1991; 1992; Pero et al., 1996) and damage isolated calf-thymus DNA directly, causing extensive oxidative damage...
- 200
- 289
- 0
Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
... flour 94 7.2.2 Hydrolysis at different temperatures 94 7.2.3 HPLC-ESI-MS Analysis 94 7.2.4 Capillary Temperature Optimum of ESI-MS analysis and MS-MS (MSn) analysis ... represents the amount before hydrolysis, open bar represents hydrolysis in a closed container in a 80° water bath, hatched bars was hydrolysis at 100° and solid bar was hydrolysis in a C C 120° dry oven ... soyasaponin A4 2.5 HYDROLYSIS Much of the early work on soyasaponins and other triterpenes such as the dammarane saponins from ginseng utilized acid or alkaline hydrolysis to deglycosolate the sugar...
- 147
- 280
- 0
Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
... EHEChemolysin, coded by the EHEC hly operon There are two different plasmid-encoded hemolysins, both members of the RTX toxin family, have been described for STEC Alphahemolysin is formed by porcine ... easily Recently, immunomagnetic particles for the separation of E coli O26 and O111 have become commercially available With the use of IMS, the isolation rate of E coli O157 has been markedly ... respectively 10 Ji-Yeon Kim et al (Nasco, USA), and placed into the icebox Upon arrival at the laboratory, samples were either analyzed immediately or held at 4oC for no longer than 24 h before analysis...
- 13
- 456
- 0
Báo cáo khoa học: " Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)" ppt
... (TEM, JME-100EA III; JEOL, Japan) Additionally, ultrathin sectioning of cultured cells was used to examine inclusion bodies with reference to a previously described method [8] The neutralization ... using the method of Reed-Muench [1] The neutralization test was performed as previously described [7] Briefly, FCWF cells were grown in 96-well flat-bottomed plates until a monolayer of cells ... recommendations and then sequenced The sequence was analyzed by GENETYX version 9.0 computer software (Software Development, Japan) and DNAMAN version 4.0 (Lynnon BioSoft, Canada) Bacteriology failed to...
- 3
- 308
- 0
Báo cáo y học: "Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)" potx
... inhibit the expression of Epstein-Barr virus (EBV) lytic proteins during the viral lytic cycle in P3HR1 cells, an oral lymphoma cell line latently infected by EBV Andrographolide inhibits the production ... BW) [101] blood glucose in STZ-induced hyperglycaemic rats (50 mg/kg BW, 10d) [115] hepatic CAT, SOD and GST activities in lymphoma bearing mice (orally 10~30 mg/d) [123] d: day; BW: body weight ... tumor in melanoma subcutaneously implanted mice (orally 200, 400 mg/kg BW, 10d) [45] Immunomodulation proliferation and IL-2 induction in hPBL [108] Anti-hyperglycemic effect plasma glucose concentrations...
- 15
- 311
- 1
Isolation and identification of components from ixeris sonchifolia hance as potential anti stroke agents
... release 101 5.2.5 RNA extraction and reverse transcription-polymerase chain reaction 102 5.2.6 Western blot analysis 104 5.2.7 Statistical analysis 105 5.3 Results 105 5.3.1 Effects of flavonoid compounds ... efficacy in stroke management But the biological targets and the underlying chemical components remained mostly unknown Accordingly, (1) the effectiveness of the herbal preparation may suffer from ... radicals not only directly damage proteins, lipids and nucleic acids, but also cause mitochondrial membrane permealization that entails cell death (Kroemer et al., 2000) Thus, the early response...
- 188
- 422
- 0
isolation, selection and identification bacillus subtilis from muddreg of beer
... EXAMINATION COMMITTEE Abstract Bacillus subtilis had widely used in many fields such as medicine, food processing, veterinary, aquaculture and especially in environmental treatment It helps disintegrate ... is approximately seconds Put a counterstain, such as basic fuchsin solution, on the slide over the bacterial smear Allow the counterstain to remain on the smear for approximately 40 to 60 seconds ... Dũng, Phạm Thị Trân Châu, Nguyễn Thanh Hiền, Lê Đình Lương, Đoàn Xuân Mượu Phạm Văn Ty.1978 Các phương pháp nghiên cứu vi sinh vật, tập III, Nxb Khoa học kỹ thuật, Hà Nội, tr.116-135 22 English Cedergren,...
- 29
- 299
- 0
Purification and cloning of PCR products
... cloning only works efficiently with thermostable DNA polymerases, such as Taq DNA polymerase, which have terminal transferase activity However, for many PCR cloning purposes Taq DNA polymerase ... the terminal transferase activity of Taq DNA polymerase that leads to the addition of an additional nucleotide, usually an A, at the 3′-end of the newly synthesized DNA strand This nontemplate-directed ... proofreading enzyme such as the Klenow fragment of DNA polymerase I, or T4 or T7 DNA polymerase, or a proofreading thermostable DNA polymerase, in the presence of the four dNTPs (see Protocol...
- 26
- 555
- 0
Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
... why they are typically maintained predominantly in either the highly methylated or the highly demethylated state This is because any repressive modification is likely to be rapidly removed if it ... models of chromatin depict chains of regularly spaced nucleosomes that fold up into a helix as highly ordered chromatin fibres However, this image is really only representative of inactive loci that ... pattern is essentially random after mobilization (A) Analysis of the intact GM-CSF locus (B) Analysis of the GM-CSF locus with a specific deletion of the 0.7 kb enhancer of regularly spaced discrete...
- 29
- 743
- 0
Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... only 0.03% and 0.2% of the wild-type activity, respectively This suggests that in the B circulans and the Alteromonas sp chitinases a negatively charged catalytic aspartate residue is absolutely ... increased only slightly, demonstrating that substrate binding is hardly affected by this mutation The Asp125Asn mutant has 50% of the wildtype hevamine activity, while its Km value is approximately ... resolution structure of hevamine, a plant chitinase/lysozyme, and analysis of the conserved sequence and structure motifs of glycosyl hydrolase family 18 J Mol Biol 262, 243–257 Read, R.J (1986) Improved...
- 9
- 616
- 0
Báo cáo " Analysis and identification of multi-variate random pressure fields using covariance and spectral proper transformations " pdf
... analysis of mean and fluctuating pressure fields around low-rise building directly measured due to turbulent flows A linkage between pattern of the pressure distribution and POD modes, especially ... analysis, synthesis and identification of the unsteady pressure fields It contributes dominantly the system energy of the pressure fields as well as contains certain frequency peaks of possibly ... modes observed differently in two cases Kikuchi et al 1997 [6] applied the POD to pressure field of tall buildings, then fluctuating pressure field was reconstructed due to only few dominant POD...
- 14
- 390
- 0
Báo cáo sinh học: "Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" pot
... glycoprotein EEV glycoprotein Profilin homolog Interact with host IL-1 EEV glycoprtn, hemagglutinin EEV glycoprotein Serpin (C16L) Capping enzyme subunit virion component virion component Early ... for analysis, and helped analyze the mass spectrometry data TSC prepared samples for analysis, analyzed the mass spectrometry data and helped to draft the manuscript SV assisted with the analysis ... amounts it does not adversely affect MS analysis, whereas, conventional detergents such as SDS and Triton X100 can greatly interfere with HPLC and mass spectrometric analysis [8] We tested the efficiency...
- 16
- 331
- 0
báo cáo hóa học:"Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" docx
... glycoprotein EEV glycoprotein Profilin homolog Interact with host IL-1 EEV glycoprtn, hemagglutinin EEV glycoprotein Serpin (C16L) Capping enzyme subunit virion component virion component Early ... for analysis, and helped analyze the mass spectrometry data TSC prepared samples for analysis, analyzed the mass spectrometry data and helped to draft the manuscript SV assisted with the analysis ... amounts it does not adversely affect MS analysis, whereas, conventional detergents such as SDS and Triton X100 can greatly interfere with HPLC and mass spectrometric analysis [8] We tested the efficiency...
- 16
- 455
- 0
báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... only slightly retarded on this column (Fig 2) and was initially identified (data not shown) by Alcian Blue staining [16] To specifically identify Muc16, we conducted Western blot analysis of Pool ... analyses The widely employed clinical serum CA125 assay was also unable to detect CA125 in spent media of MOVCAR-2 cells We therefore tested a panel of ten anti-MUC16 antibodies that we recently ... approximately 250 kDa band for Muc16 (Fig 3) The binding to Muc16 was weaker as compared to MUC16 and was usually observed at 1:250 dilution of the primary antibodies 618F and 653F specifically recognize...
- 7
- 430
- 0
Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot
... have discovered the novel and remarkably strong firbinolytic enzymes from Perionyx excavatus earthworm (family Megascolecidae) This species has been widely cultivated in Southern Vietnam for ... (USA) Page of 11 Autolysis of P excavatus Earthworm biomass was washed and finely homogenized in distilled water containing 0.1% (w/v) sodium azide Autolysis was initially performed at 45°C for ... by a 15-day autolysis at room temperature Consequently, a two-fold increase of caseinolytic activity was achieved after 10 days Precipitation of earthworm proteins from the autolysate by acetone...
- 11
- 375
- 0
Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc
... ecneuqes eht etelpmoc ot tuo deirrac eb lliw slairt eroM etis noitalysocylg laitnetop eno ylno htiw nietorp enarbmemsnart detalysocylg a si nietorp largetni sihT )%55( sullag sullaG dna )%06( ... ytiniffA giF gm 526.0 saw detule snietorpocylg lavral fo tnuoma latot ehT )3 giF( edisonirypoculg D-α lyhtem M 2.0 htiw detule erew hcihw ,snietorpocylg lavral eht yfirup ot esorahpeS-AnoC yb deifirup ... lareveS sutalunna B sulihpooB la te mikaH lE E rmA 671 .h/lm 03 fo etar wolf a ta edisonirypoculg D-α lyhtem M 2.0 htiw detule erew snietorpocylg dnuob eht dna ,4.7 Hp ,lCaN M 5.0 ,lCH-sirT Mm 02 gnisu...
- 6
- 267
- 0
Báo cáo y học: "Genome-wide prioritization of disease genes and identification of disease-disease associations from an integrated human functional linkage network" pps
... diseases still have an AUC of over 0.75, 0.8, and 0.9, respectively Additionally, we have also performed the disease centric analysis using only the area of the extreme left side of the ROC curve, which ... predictions not solely rely on the potentially biased text mining, molecular function and cellular component annotations of GO, and curated PPI data sources; other relatively unbiased sources ... links among human genes Since each data source usually characterizes only one type of functional association between genes, and covers a relatively limited set of genes, functional associations...
- 17
- 415
- 0
báo cáo khoa học: " Screening and Identification of a Renal Carcinoma Specific Peptide from a Phage Display Peptide Library" pot
... and analyzed the data statistically WWW performed immunocytochemical staining ZL performed immunohistochemical staining ZLY and ZJQ performed immunofluorescence microscopy and image analysis DCH ... the early detection of the RCC Histopathologically, although several promising biomarkers such as Carbonic anhydrase IX, B7-H1 and P53 for RCC have been under investigation, none currently have ... in the second and third rounds individually, and increasing washing with TBST for times and times in the second and third round individually Sequence Analysis of Selected Phages and Peptide Synthesis...
- 28
- 266
- 0
Báo cáo y học: "Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus" pps
... the rabbit anti-tgK polyclonal antibody Lane1 represented that the polyclonal antibody was cursorily extracted by saturated ammonium sulfate; Lane stood for the purified polyclonal antibody by ... chromatography The heavy chain and light chain were approximately 55 KDa and 22 KDa, respectively B Western blot assay Lane 1, Western blotting analysis showed that a specific band was recognized by rabbit ... Zhiwen, Chen Zhengli, Chen Xiaoyue, Wang Xiaoyu: Bioinformatics Analysis and Characteristics of Glycoprotein K Encoded by the Newly Identified UL53 Gene of Duck Enteritis Virus IEEE, the 4th International...
- 7
- 270
- 0
- chữa bệnh bằng sóng siêu âm
- nguyên lý làm sạch bằng sóng siêu âm
- nguyên lý đo lưu lượng bằng sóng siêu âm
- nguyên lý đo mức bằng sóng siêu âm
- bài 4 dựng hình ảnh và các phương pháp tạo ảnh bằng sóng siêu âm
- xử lý bằng sóng siêu âm
- phương pháp ly trích kết hợp vi sóng 4 6
- phương pháp xử lý tế bào bằng sóng siêu âm
- phương pháp khử khuẩn bằng sóng siêu âm hiệu quả diệt khuẩn tối ưu đối với môi trường
- thí nghiệm ảnh hưởng của phương pháp chiết bằng sóng siêu âm đến hàm lượng polyphenol tổng số và khả năng chống oxy hóa của dịch chiết từ lá giang
- phương pháp ly trích bằng dung môi
- phương pháp ly trích bằng enzyme
- tiền xử lý bột nấm linh chi bằng sóng siêu âm và enzyme papain
- ảnh hưởng của thời gian xử lý bằng sóng siêu âm lên hiệu suất ly trích polysaccharide từ bột nấm linh chi
- phương pháp chiết xuất curcumin bằng sóng siêu âm
Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25