pebp2beta cbfbeta in human leukemia cdna cloning of the inv 16 chimeric gene
Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
... Mapping the transcription start site of the xMGP gene Fig Sites of intron insertions within the amino-acid sequence of Xenopus, human and mouse MGPs Conserved sites of intron insertions in mammalian ... xMGP introns located within the coding region revealed that introns and are of phase I while intron is of phase II [29] The same phases are found in the corresponding introns of the mouse and human ... (Stratagene) The structure of the gene including the 5Â and 3Â anking regions was determined by double-stranded DNA sequencing, exons were identied according to the sequence of the Xenopus MGP cDNA...
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Báo cáo y học: "Expression of leukemia inhibitory factor (LIF) and its receptor gp190 in human liver and in cultured human liver myofibroblasts. Cloning of new isoforms of LIF mRNA" pdf
... as a continuous sinusoidal staining and in the stroma of portal tracts (Fig 7A) In the cirrhotic liver, the sinusoidal staining was enhanced, whereas a very faint staining was observed in fibrous ... experiments looking at STAT-3 phosphorylation JFB provided the human liver samples JFM was involved in the coordination of the project and in the critical reading of the manuscript JR conceived the study ... required to maintain the cell line; then, cells (5 × 103 per well, in 50 µl, in duplicates) were incubated in the presence of 50 µl of three-fold dilutions of cytokines or supernatant, as indicated...
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Báo cáo khoa học: Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster potx
... DmEH The oligonucleotide primers used in the PCR-based cDNA cloning are depicted in bold, and the arrows indicate orientation Nucleotides numbers are shown to the right of the sequence, and the ... position of substituted amino acids within the mEH catalytic triad (Glu in DmEH; Asp in Jheh1 and Jheh3) The large box encloses the nucleotide sequences surrounding the substituted amino acids in the ... increased, reaching a peak at the wandering stage late in the last larval stadium At the prepupal stage, EH activity declined to a level equal to that in the early time of the stadium Northern blot...
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Báo cáo Y học: Requirement of caspase and p38MAPK activation in zinc-induced apoptosis in human leukemia HL-60 cells potx
... action of Zn in induction of apoptosis remains to be unclear In this study, we investigated the molecular mechanism underlying the mode of action of the induction of apoptosis by elevation of intracellular ... stimuli to the caspase One of the most relevant aspects in the regulation of apoptosis is the involvement of mitogen-activated protein kinases (MAPKs), a family of serine/threonine kinases that ... family of cysteine proteases, play a pivotal role in the execution of apoptosis [26] We therefore examined the involvement of caspases in the apoptosis induced by Py/Zn PARP is a substrate of caspases,...
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báo cáo khoa học: "Basic Mechanisms of Arsenic Trioxide (ATO)Induced Apoptosis in Human Leukemia (HL-60) Cells" potx
... is in support of previous findings indicating that ATO induces clinical remission in a high proportion of patients with APL by inducing apoptosis [2,9] Conclusions We have demonstrated in the ... participated in the implementation of the study, and the acquisition, analysis and interpretation of data All authors read and approved the final draft of the manuscript Competing interests The authors ... subsequent inflammation [30-32] To gain insight into the mechanism of arsenic trioxide (ATO)-induced apoptosis, we examined the modulation of phosphatidylserine externalization in HL-60 promyelocytic leukemia...
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Tài liệu Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt
... HdFull-DNA are indicated as solid lines Bold lines in both sides of the cDNAs indicate primers used for the PCR Lengths of the cDNAs are shown in the parentheses (B) Structure of the genomic fragment, ... (Fig 4B) The positions of introns in the cDNA sequence are also shown in Fig The GU-AG rule in eukaryotic genes is applicable to these intron-exon junctions These results indicate that the Hdcel-1 ... itself Further, the position of intron-2 in the Hdcel-1 DNA was found to coincide to the position of corresponding intron in termite cellulase gene [19] These results strongly suggest that the HdEG66...
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Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot
... Similarly, the maximal effects of insulin on the state of tyrosinephosphorylation of the insulin receptor or of IRS1, or of phosphorylation of ERK1 ⁄ 2, was not significantly different in either group of ... involved in control of PKB, in response to insulin, which involves dual phosphorylation of PKB by insulin-activation of the phosphoinositide-dependent protein kinase-1 (PDK1) [28] and the yet ... mediators are therefore directly comparable The results demonstrate increasing insulin sensitivity downstream of the insulin receptor, probably resulting from the inherent signal amplification in the succeeding...
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Báo cáo khóa học: Furanocoumarin biosynthesis in Ammi majus L. Cloning of bergaptol O-methyltransferase ppt
... amplification of cDNA ends (RLMRACE) techniques, respectively, using gene- specific primers Cloning of the PCR products was performed by TOPO TA Cloning (Invitrogen, Karlsruhe, Germany) Briefly, the protein ... a close similarity of the cDNAs with those of other plant OMTs Therefore, the inserts were subcloned into an expression vector for the expression in E coli, and the recombinant polypeptides were ... labeled cDNAs were used as probes for Northern blotting experiments employing the total RNA of A majus cells at various time points following the addition of the Pmg elicitor The abundance of BMT...
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Báo cáo Y học: Effect of the disease-causing mutations identified in human ribonuclease (RNase) H2 on the activities and stabilities of yeast RNase H2 and archaeal RNase HII pot
... substrate binding affinity Binding analyses using BIAcore system To examine whether the mutation of Gly10 to Leu or Pro affects the substrate binding affinity of the protein, the interactions between the ... system The production levels of these subunits in the cells and the amount of the mutant proteins of Sc-RNase H2* purified from L of culture were not seriously changed regardless of the loci of the ... which the interactions between the protein and substrate are analyzed The Km values were determined in the presence of metal cofactor, whereas the KD values were determined in the absence of metal...
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Báo cáo khoa học: Adipophilin increases triglyceride storage in human macrophages by stimulation of biosynthesis and inhibition of b-oxidation doc
... However, the precise role of adipophilin in macrophage foam cell formation and, in turn, in the development of atherosclerotic lesions remains unclear In this study, we investigated the impact of adipophilin ... stimulation of lipid synthesis The effect of adipophilin on intracellular lipase activity was determined by adding the acyl-coenzyme A synthetase inhibitor triacsin C to the medium of macrophages ... Cells infected with 500 m.o.i of Ad.CMV.adipophilin then incubated in RPMI -164 0 supplemented with 10% fetal bovine serum (E) Cells infected with 500 m.o.i of Ad.CMV.GFP then incubated in RPMI -164 0...
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Báo cáo sinh học: " Open Access Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" docx
... used in the rescue of recombinant influenza will function only in primate or avian cells [5-7] In order to rescue influenza using the RNA pol I transcription machinery in canine cells, cloning the ... from the start of the 18S rRNA gene sequence The resulting MDCK clone then would be tested for RNA pol I promoter activity in vitro The sequence of the region upstream of the canine 18S rRNA initiation ... canine RNA pol I promoter was necessary In developing an approach to cloning the canine RNA pol I promoter, we examined the known features of the RNA pol I promoters and rRNA genes from other...
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Báo cáo hóa học: " Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" pdf
... used in the rescue of recombinant influenza will function only in primate or avian cells [5-7] In order to rescue influenza using the RNA pol I transcription machinery in canine cells, cloning the ... from the start of the 18S rRNA gene sequence The resulting MDCK clone then would be tested for RNA pol I promoter activity in vitro The sequence of the region upstream of the canine 18S rRNA initiation ... canine RNA pol I promoter was necessary In developing an approach to cloning the canine RNA pol I promoter, we examined the known features of the RNA pol I promoters and rRNA genes from other...
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Báo cáo khoa học: "Molecular cloning of the cDNA of canine homeodomaininteracting protein kinase 2" ppsx
... that the functions of canine HIPK2 were similar Molecular cloning of the cDNA of canine homeodomain-interacting protein kinase to those of human and murine HIPK2, because the deduced amino acid ... catalytic domain there is a glycine-rich stretch of residues in the vicinity of a lysine residue, which has been shown to be involved in ATP binding In the central part of the catalytic domain there ... Molecular cloning of the cDNA of canine homeodomain-interacting protein kinase 143 Fig Continued Spinal cord preperation A physically normal, middle-aged, mixed male dog was euthanized with 20 ml of...
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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
... aAccession number of the bacterial species in the EMBL database bLength of alignment on which the 16S rDNA inserted sequence and the corresponding sequence in the database are similar cIn the '% similarity' ... increase the risk of triggering an immune response finally culminating in synovitis However, genetic susceptibility factors of the host are also playing an important role particularly in persistent infections ... amplification of 16S rRNA genes, cloning and sequencing of bacterial DNA For 10 minutes, 500 μl of SF were centrifuged at 10,000 g The whole SF cell pellet was subjected to DNA extraction using the MagNA...
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Báo cáo y học: "Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1β-induced NF-κB-mediated inflammation and apoptosis" potx
... proinflammatory and matrix degradation gene products in chondrocytes We investigated whether resveratrol and curcumin can modulate IL-1β-induced NF-κB-regulated gene products involved in the inflammation ... observed In contrast, pre-treatment with resveratrol and curcumin resulted in a time-dependent increase of these anti-apoptotic proteins Synthesis of the housekeeping protein β-actin remained unaffected ... curcumin inhibited the expression of the above-mentioned proteins in primary chondrocytes (Figure 6) Synthesis of the housekeeping protein β-actin remained unaffected (Figure 6) Effect of resveratrol...
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báo cáo khoa học: "First-in-class, first-in-human phase I results of targeted agents: Highlights of the 2008 American Society of Clinical Oncology meeting" pot
... intra-cytoplasmic Survivin inhibitors Survivin is a member of the inhibitor of apoptosis protein (IAP) family, and has generated interest because of its increased expression in many human cancer cell lines [19] ... revolutionizing the treatment of cancer – the use of biomarkers to select individual therapies for individual patients Even from these preliminary phase I trials, where toxicity and dose-finding are the ... first -in- human agents entering the clinical arena is a further confirmation of this phenomenon Competing interests Monitoring of downstream pathways of drug targets was also presented for many of these...
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Báo cáo y học: "Human embryonal epithelial cells of the developing small intestinal crypts can express the Hodgkin-cell associated antigen Ki-1 (CD30) in spontaneous abortions during the first trimester of gestation" ppsx
... taking into account that the ratio of the area of the intestinal stroma to the area of surface epithelium Page of (page number not for citation purposes) Theoretical Biology and Medical Modelling ... cells within the intestine in all settings examined (Fig 1), with percentages varying from 3.2 to 3.9 (mean ± sd = 3.61 ± 0 .16) In the neighbouring intestinal stroma a slight cellular infiltration ... fields of the small intestines were evaluated in each case without knowledge of the clinical data (TABLE 1) Two observers examined the sections independently, and positive cellular staining for...
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Báo cáo y học: "Facing medical care problems of victims of sexual violence in Goma/Eastern Democratic Republic of the Congo"
... Our results regarding inconsistencies in post-discharge care of sexually abused females are in line with the findings of the United Nations panel reporting unmet needs of victims of sexual violence, ... violence seeking medical care in the hospitals of Goma; (2) assessment of the availability of key resources to treat victims of sexual violence in hospitals regularly caring for victims of sexual ... patients following sexual violence, these institutions were visited and included in the survey During on-site visits consisting of personal interviews and visits of key hospital facilities (e.g outpatient...
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Tài liệu USB in a Nutshell - Making Sense of the USB Standard ppt
... 200mV less than D- The polarity of the signal is inverted depending on the speed of the bus Therefore the terms ‘J’ and ‘K’ states are used in signifying the logic levels In low speed a ‘J’ state ... Packet The above diagram shows the format of an Interrupt IN and Interrupt OUT transaction • IN: The host will periodically poll the interrupt endpoint This rate of polling is specified in the endpoint ... bus topology of USB is somewhat limiting One of the original intentions of USB was to reduce the amount of cabling at the back of your PC Apple people will say the idea came from the Apple Desktop...
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An analysis of nouns formed by suffixes in english a case study of the textbook solutions pre intermedite
... little 60% of the lecturers think that their students feel interesting and 40% of them think their students not find interesting in the texts There are many short coming in students‟ learning process ... using this textbook, 32% of the students understand the texts little and 68% of the students understand so much 64% of them find the texts interesting and 36% of the students think it is unintersting ... underspecified “person having to with X” With the exact meaning of the derivative being a function of the meaning of the base and further inferencing These suffixes will be presented in this part respectively...
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