oxide ceramic functional thin layer processing by thermal and laser sintering of green layers
Báo cáo khoa học: Uptake of bilirubin into HepG2 cells assayed by thermal lens spectroscopy Function of bilitranslocase doc
... for by the activity of a carrier of bilirubin and nicotinic acid, presumably bilitranslocase Fig ICG uptake by HepG2 monolayers: effect of either antibody A or PMSF in the preincubation Monolayers ... ICG uptake by HepG2 monolayers: effects of either nicotinic acid and bilirubin (A) or taurocholate and digoxin (B) Monolayers of HepG2 cells were grown in 25-cm2 flasks After removal of the culture ... HepG2 monolayers: effect of reducing substrates in the preincubation and of the assay temperature Monolayers of HepG2 cells grown in 25-cm2 flasks were preincubated for h in the presence of either...
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Applied thin layer chromatography best practice and avoidance of mistakes 2nd ed e hahn deinstrop (wiley, 2007) WW
... the Naming and Arrangement of Precoated Layers 33 2.8 Problems in the Naming and Arrangement of Precoated Layers It should be possible to prevent selection of the wrong box of plates by recording ... applies: 2.7 Advice on the Ordering and Storage of Precoated Layers To have a clear picture of the storage of TLC precoated layers for routine and developmental work, and to comply with the GMP/GLP ... precoated layers, and advise on the choice of stationary phases in compliance with the recommendations of the pharmacopoeias 2.1 Precoated Layers – Why? In the first edition of Stahl’s laboratory handbook...
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Effect of thickness on the physical, thermal and chemical properties of ultra thin polymer films
... of Tg behaviour in which the films are considered to consist of three layers. 18, 25-27 Thus the dynamics of the material and the Tg of each layer are postulated to be different In the first layer ... buildup of polyelectrolyte multilayers via layer- by -layer assembly.35-39 Margarita et al 39 reported a method for surface modification of hydrophobic substrates through the absorption of poly(vinyl ... as a function of temperature and Tg corresponds to the discontinuous change in thermal expansion Keddie et al also prepared a series of polystyrene films on native oxide layer of silicon wafers...
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Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
... pH 7.0 and °C for h, followed by SDS ⁄ PAGE The rate of ligation was estimated from percentage intensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and 19-kDa ... form and the isolated 19-kDa propeptide are mixed; lanes d, the purified proenzyme (a mixture of the nicked and intact forms of the precursor) Proteins were detected by silver staining The band of ... time-dependent enhancement of the 57-kDa band with a concomitant diminution of the 38-kDa and 19-kDa bands, as analyzed by SDS ⁄ PAGE (Fig 3A) The 38-kDa and 19-kDa bands did not disappear completely...
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Báo cáo khoa học: Regulated expression by PPARa and unique localization of 17b-hydroxysteroid dehydrogenase type 11 protein in mouse intestine and liver pdf
... amounts of proteins (20 lg) were separated by SDS ⁄ PAGE, and expression levels of 17b-HSD11 and L-FABP were analyzed by western blotting using the specific antibodies (C) The duodenum and jejunum ... Comparison of the induction levels of 17b-HSD11 in the liver and intestine The same amounts (20 lg) of proteins from the PNS fractions of liver and small intestinal mucosa were analyzed by western ... Briefly, 0.4 lg per well of plasmid DNA was incubated with 0.8 lL per well of PlusReagent, 1.2 lL per well of Lipofectamine Reagent and 250 lL of serum free F-12 medium, and the 60% confluent cells...
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Báo cáo khoa học: High thermal and chemical stability of Thermus thermophilus seven-iron ferredoxin Linear clusters form at high pH on polypeptide unfolding doc
... nm, characteristic of the presence of secondary structure Both CD bands lose intensity as the protein unfolds (Fig 2C), and the CD spectrum on unfolding resembles that of a random-coil polypeptide ... GdnHCl-induced midpoints were 1.7 and 1.6 M GdnHCl after h of incubation and 1.1 and 1.0 M GdnHCl after 24 h of incubation for 80 and 10 lM FdTt, respectively Thermally induced FdTt unfolding In buffer at ... Understanding and probing protein stability at high temperatures and extreme conditions is relevant for a variety of biochemical and biotechnological applications Intriguingly, the stability of a...
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Báo cáo Y học: Substrate selectivity and sensitivity to inhibition by FK506 and cyclosporin A of calcineurin heterodimers composed of the a or b catalytic subunit potx
... initiated by addition of substrate, and the peptide dephosphorylation assays terminated by the addition of 100 lL of BioMol Green reagent, while the pNPP dephosphorylation assays were terminated by ... baculoviruses were screened by plaque assay and Western blotting using anti-CnAb Ig, and amplified and titered by plaque assay as described [10] The coinfection, expression and purification of baculovirus-expressed ... 50% of the observed amplitude change RESULTS Expression and purification of CaNa and CaNb CaN heterodimers composed of the Ca2+-binding B subunit and either the a or b catalytic subunit isoform...
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Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
... information towards understanding the biological relevance of YopD multimerization and even the general role of TTSS chaperone function, because functional homologues of YopD and LcrH exist in other ... NOESY spectrum with a mixing time of 150 ms The chemical shift indices are indicated by an index with values of )1, and +1, which corresponds to upfield, random coil and downfield, respectively This ... corrected by the addition of small aliquots of HCl and NaOH NMR Ó FEBS 2002 Tertiary structure of the YopD amphipathic domain (Eur J Biochem 269) 3661 experiments were also conducted between 10 and...
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báo cáo hóa học:" Ultrasonication effects on thermal and rheological properties of carbon nanotube suspensions" pot
... B and n are constants, and t is the sonication time The data in Figures 13 and 14 were fit to curves of the forms given by (Equations and 2) The values of the constants and the coefficients of ... each nanofluid sample to ensure the accuracy of the test The images recorded with the microscope were analyzed by standard image processing methods, and the average sizes of agglomerates and their ... ultrasonication time effects on sediment and the thermal conductivity of the carbon nanotubeethylene glycol nanofluids and found that thermal conductivity of the nanofluids increased with sonication...
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Báo cáo vật lý: "Compatibility, Mechanical, Thermal, and Morphological Properties of Epoxy Resin Modified with Carbonyl-Terminated Butadiene Acrylonitrile Copolymer Liquid Rubber" pps
... on the curing behaviours and adhesive strengths of an epoxy (DGEB-A) and dicyandiamide/2-methyl imidazole system was studied by Hong and Chan.11 Many works in toughening of the EP have been reported.7–11 ... CTBN and the EP, and to investigate the thermal, mechanical, and morphological properties of the modified EP EXPERIMENTAL 2.1 Materials The clear epoxy 331, with an epoxide equivalent weight of ... gel temperature, and cure temperature) of the EP are influenced by the amount of CTBN A predetermined amount of the EP was cured with a stoichiometric content of the curing agent and various CTBN...
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báo cáo khoa học: " Modification of tobacco plant development by sense and antisense expression of the tomato viroid-induced AGC VIIIa protein kinase PKV suggests involvement in gibberellin signaling" doc
... (lane 3, Figure 2A), an indication of gene silencing of the tobacco homolog of PKV and resulting from formation of doublestranded RNAs of target gene transcripts and the introduced antisense gene ... Morphology of flowers and pollen from of transformants Morphology of flowers and pollen from of transformants (A) Over expression of pkv results in reduced flower size (XS) as compared to control (X) and ... the dwarfing phenotype of XS (C) Fully mature greenhouse-grown plants showing the increased height and flowering of XAS and the dwarfing habit and reduced/delayed flowering of XS (D) Transverse...
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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
... induction Effects of a-T and its derivatives on the enhancement by TS of LPS/IFN-induced NO production We compared the effects of a-T and its derivatives TA and TN with that of TS on LPS/IFN-induced ... with Ro31-2880 and GF109203X were approximately 70 and 30%, respectively Effects of TS on the amount of PKCa We further examined the effects of TS on the amounts of PKCa and PKCb by Western blotting ... upregulation of PKCa DISCUSSION Fig Effects of PKA inhibitors (KT-5720 and H8) and PKC inhibitors (Ro31-8220 and GF109203X) on the enhancement by TS of LPS/IFNinduced NO production in VSMC (A), and Western...
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Báo cáo khoa học: Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro doc
... absorption and CD above 230 nm in UV and CD spectra of metallothioneins devoid of any aromatic residues that Fig Wavelength and time scans of the CD of MT2 in the presence and absence of NO and GSH/GSSG ... suppression of the expression and activity of inducible nitric oxide synthase (iNOS) by zinc [32,33], released from MT under the influence of nitric oxide (NO), and the scavenging of NO through ... spectroscopy was first used by Kagi and coworkers to follow the metal-binding stochiom¨ etry of MTs [42] To study the metal release by NO and its modulation by GSH and GSSG, a solution of 100 lM Cd5Zn2-MT2...
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Báo cáo khoa học: "Deep Syntactic Processing by Combining Shallow Methods" ppt
... 2001 Joint and conditional estimation of tagging and parsing models In Proceedings of the 39th Annual Meeting of the Association for Computational Linguistics and the 10th Conference of the European ... Madrid James R Curran and Stephen Clark 2003 Investigating GIS and smoothing for maximum entropy taggers In Proceedings of the 11th Annual Meeting of the European Chapter of the Association for ... words before and after it) When evaluating antecedent recovery, the EE s are regarded as four-tuples, consisting of the type of the EE , its location, the type of its antecedent and the location(s)...
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Morphology and growth mechanism study of self assembled silicon nanowires synthesized by thermal evaporation
... made of a Si core and a SiO2 sheath The one-dimensional growth of SiNWs is facilitated by the SiO2 sheath that con®nes the lateral growth of SiNWs, and the high absorptivity for Si±O clusters of ... formation of a sucient amount of silicon atoms and/ or silicon oxide clusters in gas phase from the target powders of silicon or silicon oxide by laser ablation or high temperature evaporation ... oven -laser ablation [10±13] and thermal evaporation [14±17] are two methods normally used for the synthesis of SiNWs The key point in these two methods is the formation of a sucient amount of...
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Large scale synthesis, characterization and photoluminescence properties of amorphous silica nanowires by thermal evaporation of silicon monoxide
... in the form of wires having hundreds of micrometers length is clearly seen Fig 2(a) also reveals that several layers of nanowires were deposited one over another and total thickness of the film ... diffusion of Si/SiOx vapors into the metal particles, (ii) formation of liquid droplet of metal and Si/SiOx and (iii) the precipitation in the form of solid nanowires after super-saturation of liquid ... of crystalline Si core and amorphous SiO2 outer shell structure as confirmed by HRTEM image and SAED pattern On the other hand, the formation of crystalline Si–SiO2 core-shell nanowires first and...
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Silicon nanowires fabricated by thermal evaporation of silicon monoxide
... structure [20] Some SiO2 and -Al2 O3 came from the decomposition of SiO and the Al2 O3 substrate, respectively Fig TEM images of the SiNWs produced by thermal evaporation of SiO on a silicon wafer ... low-energy places of silicon wafers such as defects With the increase of Si atoms, the nuclei will grow up to wires And because of oxide reaction and growth energy, some growth directions of SiNWs will ... SiNWs on silicon wafers and Al2 O3 templates were fabricated by the thermal evaporation of silicon monoxide (SiO), respectively The SiNWs were about ∼30 nm in diameter and tens of micrometers in length...
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Sulfide assisted growth of silicon nano wires by thermal evaporation of sulfur powders
... and 60225010) and the Key Project of Chinese Ministry of Education The authors would like to thank Prof Youwen Wang and Mr Z.C Chen for their great help in the measurements of TEM and Raman spectroscopy ... oxidation of nanowires This suggests that the nanowires were composed of silicon and silicon oxide as sheath Actually, some SiNWs could be oxidized to be silicon oxide nanowires because of the low-vacuum ... diameter of B20 nm were successfully synthesized on silicon wafers by thermal evaporation of sulfur powders It is considered that the decomposition of SiS resulted in the formation of SiNWs Furthermore,...
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Synthesis and photoluminescence property of silicon carbon nanowires synthesized by the thermal evaporation method
... ratio of Si/C/O of the nanowires is about 3:2:2, which corresponds well to the standard SiC and SiO2 The small quantity of oxygen may come from the resident oxide layer The IR spectrum of the ... reveals that large quantities of randomly distributed wire-like products have been obtained The nanowires are of uniform diameter of about 50 nm and lengths up to tens of micrometers In addition, ... to room temperature, and the AAO template surface was deposited with thick layer of light-blue fluffylike products Morphology and crystal lattice of the samples were observed by field-emission transmission...
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Temperature-Controlled Growth of Silicon-Based Nanostructures by Thermal Evaporation of SiO Powders
... ball covered with a thin amorphous silicon oxide layer, while the arm is a crystal Si wire sheathed with a thick amorphous outer layer of silicon oxide The composition of silicon oxide has been determined ... is often observed (Figure 6b) Both TEM examination and EDS analysis reveal that the tip of the pinlike nanowire is composed of a crystalline Si ball and a very thin amorphous silicon oxide layer, ... Si ball covered with a thin silicon oxide layer and the wire has a Si core and a thick silicon oxide outer sheath In zone I, there also exist many nanowires on the surface of the round-tip rods,...
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