overexpression purification and in vivo reconstitution of hexahistidine tagged wild type and b d186 433 cor
Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
... lung, and < /b> kidney were fixed in < /b> 10% phosphate buffered formalin, embedded in < /b> paraffin, sectioned, and < /b> stained with hematoxylin and < /b> eosin (H&E) In < /b> vivo < /b> pharmacokinetics and < /b> bioavailability In < /b> vivo < /b> ... the stability and < /b> toxicity of < /b> PANs, and < /b> further investigated the sustained release behavior in < /b> vivo < /b> of < /b> drug loading in < /b> PANs PA was firstly synthesized and < /b> characterized by FT-IR and < /b> 1H NMR, and < /b> ... clearance in < /b> mice: the effect of < /b> a separate and < /b> combined presence of < /b> surface charge and < /b> polymer coating Int J Pharm 240:95–102 Li, S.D., Huang, L (2008) Pharmacokinetics and < /b> biodistribution of < /b> nanoparticles...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... expected Finally, UP12 expression in < /b> the mutant and < /b> wild-< /b> type < /b> cells was examined by Western blotting, as described below Purification of < /b> UP12 and < /b> preparation of < /b> anti-UP12 Ig The UP12 hybrid containing ... Luria–Bertani broth Single colonies of < /b> wild-< /b> type < /b> and < /b> D ybdQ strains were plated on Luria–Bertani agar plates After overnight incubation, several colonies of < /b> each strain were suspended in < /b> Luria–Bertani ... memory of < /b> the late Professor Alexander Girshovich who initiated and < /b> was actively involved in < /b> the beginning of < /b> this work We wish to thank I Gokhman for her help during the subcloning of < /b> YbdQ This...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... Tricine ⁄ NaOH, pH 7.5, 0.06% dodecyl -b- maltoside PSI incubations were stopped by addition of < /b> soybean trypsin inhibitor (Sigma type < /b> I-S) to a final concentration of < /b> mgÆmL)1 and < /b> boiling loading buffer ... degradation product of < /b> membrane-inserted Lhcb1 digested by thermolysin or trypsin and < /b> ⁄ or its interaction with other proteins such as the PSI core Incorporation of < /b> tagged < /b> PSI-G into PSI Whilst it ... pools of < /b> plants expressing similar levels of < /b> PSI-G, and < /b> analysed by immunoblotting, using antibodies to PSI-G and < /b> PSI-F, as an indicator of < /b> the relative content of < /b> PSI in < /b> the samples Plants lacking...
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Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
... [27] In < /b> rats Fig Western blot analysis of < /b> p53 and < /b> c-Jun in < /b> liver and < /b> lung Total protein extracts were obtained as described in < /b> experimental procedures A single band of < /b> about 53 kDa was detected in < /b> ... gene and < /b> 0.9-kb of < /b> mRNA c-H-Ras gene) Equal loading of < /b> the gels was assessed using ethidium bromide staining of < /b> the gel The probes were labeled with [a32P]dCTP (3000 CiÆmmol)1) by random priming ... aorta in < /b> heparinized syringes and < /b> then liver and < /b> lung samples were taken and < /b> processed immediately Retinol and < /b> retinyl palmitate were measured in < /b> serum and < /b> tissues following the method described by...
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Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx
... 1.13 kb of < /b> GO cDNA was inserted into the EcoRI site of < /b> the MCS of < /b> pT7-DBam/Hind plasmid [6] The original ATG starting codon is shown in < /b> bold and < /b> the ATG codon corresponding to the Met1 of < /b> the wild-< /b> type < /b> ... recombinant enzyme catalysed the oxidation of < /b> sarcosine and < /b> glycine (specific activity of < /b> 0.57 and < /b> 0.43 UÆmg)1 protein, at 25 °C) Table Purification of < /b> recombinant B subtilis glycine oxidase from BL21(DE3)pLysS ... residue, which is involved in < /b> the recognition/binding of < /b> the substrate in < /b> both MSOX and < /b> DAAO [12,15], is not conserved in < /b> GO and < /b> probably represents a key element for defining its substrate specificity...
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Báo cáo khoa học: A study of microRNAs in silico and in vivo: bioimaging of microRNA biogenesis and regulation doc
... can be used for the in < /b> vitro and < /b> in < /b> vivo < /b> investigations of < /b> miRNA production patterns Second, noninvasive monitoring of < /b> miRNAs provides useful information of < /b> the possible role of < /b> miRNAs involving ... used to create a better understanding of < /b> the production and < /b> function of < /b> miRNA in < /b> vivo < /b> as well as for monitoring the therapeutic potential of < /b> miRNAs in < /b> cancer Bioimaging of < /b> microRNA biogenesis The ... identified to be one of < /b> the endogenous targets of < /b> miRNA221 that is highly expressed in < /b> papillary thyroid carcinoma The in < /b> vivo < /b> bioluminescent signals from the 3¢-UTR region of < /b> homeobox B5 containing the...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
... determining the in < /b> vivo < /b> resistance of < /b> NOS and < /b> HSP90 to calpain degradation Results Levels of < /b> HSP90 and < /b> NOS isozymes in < /b> rat brain and < /b> aorta The level of < /b> HSP90 and < /b> the type < /b> of < /b> NOS isoform present in < /b> ... FEBS M Averna et al In < /b> vivo < /b> degradation of < /b> NOS and < /b> HSP90 by calpain A B C D Fig In < /b> vivo < /b> digestion of < /b> NOS and < /b> HSP90 in < /b> HMS rats during HSD treatment Aliquots (20 lg protein) of < /b> rat brain soluble ... 1204–1212 24 Bellocq A, Doublier S, Suberville S, Perez J, Escoubet B, Fouqueray B, Puyol DR & Baud L (1999) Somatostatin increases glucocorticoid binding and < /b> signalling in < /b> macrophages by blocking the...
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báo cáo hóa học:" Function of anterior talofibular and calcaneofibular ligaments during in-vivo motion of the ankle joint complex" docx
... height of < /b> 175 cm and < /b> average body mass index (BMI) of < /b> 21.8 kg/m2) were included in < /b> this study None of < /b> the participating subjects had subjective complaints of < /b> ankle pain, previous history of < /b> ankle injury ... inversion and < /b> internal rotation Strain in < /b> the CFL was found to increase in < /b> dorsiflexion and < /b> inversion Ozeki et al.[7] investigated strain changes in < /b> central fibers of < /b> the lateral ankle ligaments in < /b> ... understanding of < /b> function of < /b> these ligaments and < /b> injury mechanisms However, the in-< /b> vivo < /b> behavior of < /b> the lateral ankle ligaments during the range of < /b> motion has not been well studied Data in < /b> this...
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Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs doc
... 2004) Bacillus species, a type < /b> of < /b> exogenous spore-forming bacteria, are not normally found in < /b> the gastrointestinal tract but have also been shown to be effective in < /b> keeping a favorable balance of < /b> ... considerable variability in < /b> their ability to inhibit the growth of < /b> E coli K88 Based on their high inhibitory activity against E coli K88 during the initial screening, six Bacillus strains were selected ... 90:139–146 Table Effect of < /b> simulated gastric fluid on the viability of < /b> six Bacillus strains during a h incubation t (n=3)* Strains Viable counts (log CFU/ml) 0h Bacillus spp 23 B subtilis MA139 Bacillus...
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Báo cáo toán học: "OXIDATIVE PHOSPHORYLATION: Kinetic and Thermodynamic Correlation between Electron Flow, Proton Translocation, Oxygen Consumption and ATP Synthesis under Close to In Vivo Concentrations of Oxygen" docx
... “with Pi labeled with 18O and < /b> 32P and < /b> unlabeled ATP in < /b> the presence or absence of < /b> substrates or of < /b> oxidation inhibitors” [8], 18O is incorporated into Pi during the period of < /b> equilibrium in < /b> which ... experiment was initiated by adding 400 nmols of < /b> ADP and < /b> 6.3 nmols of < /b> ATP (as contaminant of < /b> ADP) to an air-saturated medium free from RLM and < /b> succinate After 1.5 of < /b> incubation, 1.0 mg of < /b> RLM protein was ... reactions were initiated by consecutively adding 18.4 nmols of < /b> O in < /b> (a), 2.76 in < /b> (b) and < /b> 0.92 in < /b> (c) The time course of < /b> both O2 consumption and < /b> ATP synthesis were simultaneously recorded during the...
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Báo cáo y học: "The in vivo expression of actin/salt-resistant hyperactive DNase I inhibits the development of anti-ssDNA and anti-histone autoantibodies in a murine model of systemic lupus erythematosus" ppt
... from Pharmingen-Becton Dickinson (San Diego, CA, USA) Biotinylated antibodies were detected using an allophycocyanin-conjugated streptavidin antibody (Pharmingen-Becton Dickinson) Staining was ... activity was mainly measured from urine instead of < /b> serum because the absence in < /b> urine of < /b> natural inhibitors of < /b> DNase activity such as G-actin To test if the expression of < /b> DNase I from T cells in < /b> the ... by the development of < /b> a range of < /b> autoantibodies of < /b> both the IgM and < /b> IgG isotypes, and < /b> subsequent glomerular disease [37,38,49,50] In < /b> our hands, high levels of < /b> IgM anti-ssDNA antibodies were induced...
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Báo cáo khoa học: "Biological in-vivo measurement of dose distribution in patients’ lymphocytes by gamma-H2AX immunofluorescence staining: 3D conformal- vs. step-and-shoot IMRT of the prostate gland" ppsx
... this rule are described and < /b> due to different irradiation conditions or different kinds of < /b> ionizing irradiation [18] Page of < /b> DLH 15 10 Proband Proband Proband Proband Proband Regression 3D IMRT ... measuring the actual dose distribution On the other hand, table time in < /b> both modalities differs by factor 1.4 During 11.5 vs 16.3 of < /b> table time, lymphocytes in < /b> both groups have the chance of < /b> being ... contains beams, whereas in < /b> SSIMRT, dose is distributed within 7-9 beams The distribution of < /b> low doses is broader in < /b> a larger volume in < /b> SSIMRT Using the gamma-H2AX stain to detect DNA-double strand...
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Báo cáo khoa học: " In vivo assessment of catheter positioning accuracy and prolonged irradiation time on liver tolerance dose after single-fraction 192 Ir high-dose-rate brachytherapy" pot
... number of < /b> dose-contributing catheters increases Therefore, the catheter contribution index increases with the number of < /b> contributing catheters In < /b> case of < /b> a single contributing catheter, IP = In < /b> ... dysfunction and < /b> interstitial edema and < /b> the corresponding follow-up dates are displayed in < /b> Table The mean of < /b> AC I is > in < /b> each subgroup, indicating that the catheter contribution index in < /b> “MA+” is ... Based on metric analysis of < /b> target mobility and < /b> set-up inaccuracy in < /b> the CT simulation prior to or during treatment, safety margins for defining the planning target volume (PTV) of < /b> about mm in...
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Báo cáo khoa hoc:" Biodegradable Nanoparticles are Excellent Vehicle for Site Directed in-vivo Delivery of Drugs and Vaccines" pptx
... facilitate bone healing by using PLGA nanoparticles containing therapeutic genes such as bone morphogenic protein Nanoparticles for drug delivery into the brain: The blood-brain barrier (BBB) is the ... release profiles for a number of < /b> drugs, vaccines and < /b> biomolecules to be used in < /b> a variety of < /b> applications in < /b> the field of < /b> medicine In < /b> this manuscript, the methods of < /b> preparation of < /b> biodegradable NPs, ... caused by presence of < /b> high concentration of < /b> polysorbate 80 [64] OX26 MAbs (antitransferrin receptor MAbs), the most studied BBB targeting antibody, have been used to enhance the BBB penetration of...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... PBS 1% bovine serum albumin (BSA) and < /b> incubated with anti-CD4-FITC and < /b> anti-CD8-Per-CP antibodies (eBioscience) for 30 minutes and < /b> then washed twice with PBS 1% BSA followed by detection of < /b> incorporated ... expression of < /b> PD-L1, iNOS, and < /b> COX-2 in < /b> MSCs, making these molecules candidate mediators of < /b> T-cell inhibition The involvement of < /b> iNOS and < /b> COX-2 in < /b> inhibition of < /b> T-cell proliferation was demonstrated by ... cytokines have been shown to synergistically induce the expression of < /b> iNOS [34] and < /b> IDO [35] in < /b> fibroblasts The expression of < /b> iNOS, IDO, PD-L1, and < /b> COX-2, molecules involved in < /b> inhibition of < /b> T-cell...
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Báo cáo y học: " In vivo imaging of the airway wall in asthma: fibered confocal fluorescence microscopy in relation to histology and lung function" potx
... a bundle of < /b> optical microfibres to the tip of < /b> the miniprobe of < /b> mm in < /b> diameter, which can be inserted into the working channel of < /b> a fibreoptic bronchoscope [14] High-quality and < /b> real-time in < /b> vivo < /b> ... ‘optical biopsies’ of < /b> the airway wall are obtained by placing the tip of < /b> the miniprobe onto the airway wall surface Another advantage of < /b> FCFM and < /b> its miniprobe is the ability to reach and < /b> therefore ... where the miniprobe had been placed before (Figure 1b, d) Directly after collection, the biopsy specimen was fixed in < /b> 4% buffered formaldehyde and < /b> embedded in < /b> paraffin Elastic fibres and < /b> FCFM pattern...
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Báo cáo khoa học: "Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains" pptx
... available In < /b> the case of < /b> animal 191, as discussed above, the reason could have been the lack of < /b> infection Page of < /b> Conclusions The in < /b> vitro and < /b> in < /b> vivo < /b> properties of < /b> two strains of < /b> BoHV-5 belonging ... A663 strain (BoHV-5 subtype b ) possesses diminished capacities concerning cell lysis and < /b> cell-tocell spread in < /b> comparison with the N569 strain (BoHV5 subtype “a”) In < /b> addition, the in < /b> vivo < /b> properties ... background of < /b> N569 and < /b> A663 strains is unknown and < /b> we are not able to rule out the possibility of < /b> genomic differences having an impact on the in < /b> vitro behavior of < /b> both BoHV-5 strains Concerning...
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Báo cáo y học: "In vitro and in vivo evaluation of a new active heat moisture exchange" potx
... [16,30], causing an increase in < /b> carbon dioxide levels, especially during low tidal volume ventilation [31] HMEs also cause an increase the inspiratory work of < /b> breathing, with an increase in < /b> intrinsic ... used this offset (in < /b> the order of < /b> 0.1–0.2°C) to correct the measurements obtained during the study In < /b> each condition, the average of < /b> three or four readings from the wet and < /b> dry probe was computed ... 3) At a minute ventilation of < /b> 10 l/min, passive Performer, Hygrobac and < /b> Hygroster all had significantly higher temperature and < /b> AH than at minute ventilations of < /b> and < /b> 15 l/min Increasing the tidal...
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Báo cáo y học: "Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications" ppsx
... illustrating resorufin fluorescence (red) in < /b> the liver (L) and < /b> gall bladder (GB) (C1 and < /b> C2) In < /b> vivo < /b> imaging of < /b> region of < /b> interest in < /b> frame B3 (white square) showing in < /b> vivo < /b> phenotype of < /b> hepatic ... via gill, in < /b> vivo < /b> metabolism in < /b> gill and < /b> gut, good for investigating blood to bile transport, IHBPs, EHBPs and < /b> intestinal lumen 10–50 μM 7-Ethoxyresorufin DMSO 30/45 In < /b> vivo < /b> imaging of < /b> CYP1A activity ... Tryptophan Hydroxykynurenine glucoside (3-HGK) Serotonin (5-HT) HIAA α-napthol Bilirubin Protoporphyrin Collagen I Elastin Lipofuscin Vitamin A Vitamin E Thiamin (B1 ) Riboflavin (B2 ) Salicylic Acid...
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Báo cáo sinh học: "A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma" ppsx
... result of < /b> steric hindrance and < /b> might be substantially reduced by selecting another linker sequence Longer intervening sequences as well as introduction of < /b> flexible polyglycine linkers may contribute ... [4-8], implying that transfer and < /b> distribution of < /b> the transgene in < /b> human brain tumors were very low in < /b> vivo < /b> and < /b> differed obviously from the findings in < /b> animal experiments Presently, the standard method ... using fluorescence and < /b> bioluminescence were introduced and < /b> increasingly used for temporal and < /b> spatial monitoring of < /b> transgene expression [11-13] Bioluminescence imaging (BLI) using luciferase (Luc)...
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