oreochromis sp in yeast pichia pastoris and its stimulatory effects on food intake and growth
Báo cáo lâm nghiệp: "Occurrence of Ips duplicatus (Coleoptera: Curculionidae, Scolytinae) on pines (Pinus sp.) in the Czech Republic and southern Poland – Short Communication" pdf
... Turcz ex Trautv.), exceptionally on fir (Abies sp. ) and rarely on juniper (Juniperus sp. ) Pfeffer (1989) summarized only spruces and pines (Picea obovata, Picea abies (in orig Picea excelsa), Picea ... 1993, Pinus sylvestris, trees infested densely along the whole stem length from top to crown, felled in pine-spruce stands damaged by wind in 1990 (W. Szczepański, personal communication) I duplicatus ... high population densities In the study area in the Czech Republic, spruces are declining because of drought and honey fungus attacks and 50–60% (n = 635) of them are consequently infested by I. duplicatus...
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Báo cáo khoa học: "Pregnancy loss in dairy cows: the contributing factors, the effects on reproductive performance and the economic impact" doc
... dedulcni LP fo noitinifed ehT ]71[ sisongaid ycnangerp evitisop a rof noiretirc eht saw yhpargonosartlu yb sutef ro oyrbme ,elcisev eht fo noitingoceR noitaplap launam dna yhpargonosartlu htob gnimrofrep ... lamron rof kcehc ot yhpargonosartlu dna noitaplap latcer yb denimaxe saw woc LP hcae fo tcart evitcudorper ehT ]53,7[ yduts siht ni ssol latef dna ssol cinoyrbme etal htob dedulcni LP ,suhT sisongaid ... A LP tuohtiw LP htiw tnangerp metI swoc oN swoc oN swoc oN swoc yriad ni )LP( ssol ycnangerp rof srotcaf ksir fo sisylana eht ni dedulcni atad eht rof scitsitats evitpircseD elba T elbaT selbairav...
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A role for chondroitin sulfate proteoglycan in regulating the survival and growth of neural stem cells
... formation .120 3.7.1.6 Notch is involved in neurosphere formation and proliferation 122 3.7.1.7 Shh is involved in neurosphere formation and proliferation .125 3.7.1.8 Phosphatases are involved in ... the midline and continue to expand, eventually giving rise to the brain (Purves, 2001) Introduction: 18 Figure 1.7 Regional specification of the developing brain (A) Early in gestation the neural ... CSPG stimulates NSC survival CSPG also increases neurosphere size and reduces the population doubling time of neurospheres in culture, indicating that CSPG stimulates proliferation In addition,...
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Báo cáo y học: " Chronic obstructive pulmonary disease, asthma and protective effects of food intake: from hypothesis to evidence?" docx
... vitamin C intake and lung function in rural China Am J Epidemiol 1998, 148:594-599 Butland BK, Strachan DP, Anderson HR: Fresh fruit intake and asthma symptoms in young British adults: confounding ... fish intake on lung function was reported in several studies in the mid-1990s, but findings on the association with respiratory symptoms were conflicting [4,5,18,19] More recent observational ... unfavourable intake of these foods [20] The contribution of each of these foods to the overall beneficial effect was largely additive Information on the temporal relationship between dietary intake and...
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Báo cáo Y học: Recombinant pronapin precursor produced in Pichia pastoris displays structural and immunologic equivalent properties to its mature product isolated from rapeseed pptx
... proBnIb-encoding region The deduced amino-acid sequence is also shown The sequence contained in a box corresponds to the IPF connecting both chains The sequences used as primers in the PCR cloning are ... natural and recombinant proteins Polyclonal sera against recombinant pronapin BnIb and Sin a 1, the major allergen from yellow mustard seeds, were prepared by immunizing New Zealand white rabbits ... NT2SAs In HMW-napins, Sin a and castor bean NT2SA, this is a 15-residue segment and 13 amino acids in arabidin (Fig 2B) Only Ric c contains a short IPF with an amino-acid sequence (Ser-Asp-Asn)...
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Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf
... aprotinin, leupeptin and pepstatin, twice in kinase buffer, and used for in vitro kinase assays In vitro kinase assays Recombinant human protein kinase CK2 holoenzyme (0.5 U, corresponding to ... that the C-terminal portion of Rio1p was involved in protein–protein inter- Fig Co-immunopurification experiments and identification of domains essential for Rio1p–Cka2p interactions (A) HA3-tagged ... 402 in the catalytic domain and the presence of several phosphorylation sites for CK2 in the C-terminal portion of Rio1p, one (or more) of them in the segment between positions 402 and 408 In...
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Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elafin and pre-elafin (trappin-2) expressed in Pichia pastoris ppt
... rec-trappin-2 concentration Recombinant elan (rec-elan) and recombinant trappin-2 (rec-trappin-2) preparations were active site titrated using HNE Reaction mixtures (990 lL) containing constant ... PPE and rec-elan or rectrappin-2 Equilibrium dissociation constants governing the interaction between PPE and rec-elan or rec-trappin2 were determined using titration experiments Increasing concentrations ... from the main peak was analyzed by high-resolution Tricine SDS/PAGE which revealed a single protein of about kDa and 12 kDa for elan in reducing and nonreducing conditions, respectively, and 12 kDa...
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... (isoquercitrin) Quercetin-3-GlcRha (rutin) Quercetin-4¢-GlA Kaempferol-3-Glc Isorhamnetin-3-Glc Naringenin-7-GlcRha (naringin) Hesperetin-7-GlcRha (hesperidin) Phloretin-7-Glc (phlorizin) Oleuropein Cyanidin-3-Glc ... containing arbutin or salicin as substrate; 100% C initial, increasing D to 20% (10 min), 50% (15 min), 100% (5 min), held at 100% (5 min); cyanodin glycosides; 5% B/95% A initial (5 min), increasing ... 3¢-¯anking regions) will facilitate future studies concerned with controls of expression for CBG In conclusion, a human cDNA encoding CBG has been cloned and expressed in the yeast P pastoris and...
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Báo cáo Y học: Brassica napus soluble epoxide hydrolase (BNSEH1) Cloning and characterization of the recombinant enzyme expressed in Pichia pastoris docx
... 3¢) containing five His codons, a BamH1 site, a Kozak consensus sequence for a proper translation initiation in P pastoris and also the nine initial bases missing in the isolated cDNA clone The ... a linear decrease in activity at increasing concentrations of the solvent (Fig 5B) Quercitin and chalcone oxide had weaker effects but caused 75% and 98% inhibition at mM concentration, respectively ... described [3] The routine assays contained 100 lM final substrate concentration in 0.1 M potassium phosphate, pH 7.0 and were run at Expression in yeast and purification of recombinant BNSEH1 The BNSEH1...
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Báo cáo khoa hoc:" CP-31398, a putative p53-stabilizing molecule tested in mammalian cells and in yeast for its effects on p53 transcriptional activity" potx
... detected in yeast cells containing the other point mutant variants Average and standard deviation were determined from three independent experiments (C) Growth on selective plates containing 20 mM ... CH: Latent and active p53 are identical in conformation Nat Struct Biol 2001, 8:756-60 Espinosa JM, Emerson BM: Transcriptional regulation by p53 through intrinsic DNA/chromatin binding and site-directed ... the integrated lacZ and HIS3 reporter genes, which resulted in increased β-galactosidase activity (figure 1B) and cell growth on plates lacking histidine and containing 20 mM 3-AT (figure 1C) In...
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Globalization and its effects on the development of educational service in Vietnam
... training Thus, continued participation in export markets requires continuous skill upgrading through training There is also increasing interest in the impact of global value chains on upgrading ... role in international relations o Interdependence amongst national economies has increased o In international relations, there have always existed two dimensions: cooperation and competition 1.2Education ... The growing internationalisation of business education can help the spread of best practice techniques and internationally recognised standards in business education International education providers...
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Tài liệu Báo cáo khoa học: L-Arabinose transport and catabolism in yeast doc
... l-arabinose content [24,27] Our interest in l-arabinose fermentation in yeast led us to screen a collection of strains and recent isolates from wood-rich environments Two yeast strains, C arabinofermentans ... L-Arabinose transport and catabolism in yeast tinct transport systems operating simultaneously and differing in substrate affinity (half-saturation constants of 125 mm and 0.1 mm for low-affinity and ... d-xylose and d-glucose in P guilliermondii and particularly by d-xylose in C arabinofermentans, demonstrating its lower specificity in comparison with the l-arabinose facilitator In C arabinofermentans,...
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Tài liệu Báo cáo khoa học: Oligomerization of the Mg2+-transport proteins Alr1p and Alr2p in yeast plasma membrane pptx
... reduction in growth and in cellular free Mg2+ Finally, effects of C-terminal deletions including one or both TM domains (alr-c96 and alr-c137, respectively) resulted in growth phenotypes and Mg2+ contents ... with increasing methionine concentrations was taken as an internal control to exclude Fig Interactions of Alr1p and Alr2p in the split-ubiquitin system Alr1p and Alr2p were analyzed using the split-ubiquitin ... dropped in equal amounts on to plates lacking histidine and adenine with increasing methionine concentrations (A) Interactions between Alr1–Alr1 pairs, Alr2–Alr2 pairs and Alr1–Alr2 pairs Controls...
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Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx
... microsomes on protein, K+ and valinomycin concentrations Uptake of 29.5 lm BSP was found to linearly depend on the addition of protein (2.6 ± 0.04 lmolÆmin)1Æmg protein)1, with lg valinomycin), as ... activity inhibition The inhibition rate constants can be related to bilirubin concentration by the Scrutton and Utter equation [40]: Fig The dependence of the valinomycin-induced BSP uptake rate into ... bilirubin binding only in the liver, but not in carnation (Table 1, section D) Conversely, antibody B identifies a site involved in the high-affinity binding of cyanidin 3-glucoside in carnation but...
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Tài liệu Báo cáo khoa học: Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites doc
... 000 g for 30 at °C The supernatants containing recombinant proteins of AsPPase were purified using ProBondTM resin (Invitrogen) under nondenaturing conditions and subsequently eluted with a stepwise ... (Michaelis constant) and Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins in the standard reaction mixture in the presence of increasing concentrations of ... decapitation and the lungs were removed and minced with a surgical knife The minced tissue was wrapped in cotton gauze and suspended in NaCl/Pi containing 100 lgÆmL)1 penicillin/ streptomycin at...
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Tài liệu Economic Reforms, Foreign Direct Investment and its Economic Effects in India pdf
... Review of Economics and Finance 40: 337-354 Gupta, S (2005) Multinationals and Foreign Direct Investment in India and China In: E.M Graham (ed.), Multinationals and Foreign Investment in Economic ... our findings on manufacturing are in line with the cross-country findings on FDI and trade reported in Aizenman and Noy (2005) Using Granger causality tests on disaggregated measures of financial ... By using industry-specific FDI and output data and applying a panel cointegration 28 For instance, the contribution of communication services was less than five percent in 2000 (RBI online data)...
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Báo cáo khoa học: Amino acids Thr56 and Thr58 are not essential for elongation factor 2 function in yeast potx
... serine (Fig 2) Mutants containing asparagine, aspartic acid, glycine, lysine or valine were nonfunctional (Fig 2) Clones expressing eEF2 in which the adjacent threonine T58 was replaced by amino ... by recombination using LR clonase The destination vector was transformed into strain YOR133w for confirming gene expression, and into strain GB1 for functional analysis by plasmid shuffling A copy ... Comparison of the amino acid context surrounding the putative phosphorylation site in eEF2 from various fungi, plants and metazoans The position of threonines T56 and T58 (yeast numbering) are indicated...
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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx
... characterization of the yeast I7 OR To examine the presence of the I7 protein in uninduced and induced yeast cells, membrane preparations 526 J Minic et al Fig Modifications of yeast pheromone and signal ... corresponds to the receptor monomer The 51 kDa band may correspond to a glycosylated form of the receptor since I7 OR contains two N-glycosylation sites, one at the N terminus and another in the ... subunit in comparison to the strain coexpressing the Ga15 subunit when grown in the same conditions 527 Expression of olfactory receptors in yeast for screening A J Minic et al and Ga15 in membrane...
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Báo cáo khoa học: Oligosaccharide synthesis in Fibrobacter succinogenes S85 and its modulation by the substrate potx
... Incubation in medium containing soluble sugars 25 20 15 10 20 time (min) 30 40 Fig Time dependence of sugar consumption (A) and metabolite production (B) Fibrobacter succinogenes cells were incubated ... supernatant or in the cell extracts, following incubation with glucose Maltose was not detected in cell extracts following incubation with cellobiose, or glucose and cellobiose In the corresponding supernatants, ... the incubation were taken at 1, and h, and extracellular and intracellular media were separated by centrifugation and analysed in parallel by 1H NMR spectroscopy and by TLC Under these conditions,...
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Báo cáo khoa học: Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris ppt
... galactose-binding and the mannose-binding lectins In contrast to the galactose-binding JRLs that are synthesized on the endoplasmic reticulum and follow the secretory pathway to accumulate in protein ... Expression of Orysata in Pichia pastoris The EasySelect Pichia Expression Kit from Invitrogen was used to clone and express Orysata in the P pastoris strain X-33 To achieve secretion of the recombinant ... carbohydrate-binding site and hence is unlikely to interfere with the carbohydratebinding properties of the lectin (results not shown) Agglutination activity and carbohydrate-binding properties of recombinant...
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