... form of miR9 To detect the transcript level of pri-miR9, the upstreamregion of pri-miR9-1 was isolated from the genomic DNA of HeLa cells and cloned into a promoterless vector,pGL3_Basic vector ... amplifiedusing the primer pairs listed in Table 1, and then sequenced to determine the orientation ofthe fragments in the repor-ter vector. These constructs were then transfected into P19cells ... for the transcriptionfactors that are required to maintain the neuronaldifferentiation of stem cells.Moreover, the molecular mechanisms ofthe bioge-neses of a number of miRs are equivocal....
... red. C1 of the hexanucleotide is at the bottom left and the target adenine is at the top of each model. The sidechains of RTA are shown in blue. (A) Model 1 has the tetraloop bound in the active ... determine whether the reduction inactivity of this RTA variant could be attributed solely to the change in charge and size ofthe sidechain of this singleresidue. The structure of RTA R213D is essentially ... with the first ofthe models proposed byMonzingo and Robertus [10].In order to understand more fully the precise role of Asn122 in the catalytic activity of RTA, it would be of value to solve the...
... half ofthe year, down from net inflows of EUR 142 billion for the same period in 2010. This reduction reflects the change in investor confidence from a high level at the beginning of 2010 to ... during the second quarter of 2011, compared to EUR 30 billion in the first quarter. This reduction was attributable to a rise in net outflows from money market funds, from EUR 9 billion in the ... into UCITS amounted to EUR 48 billion during the first half of 2011, slightly behind the EUR 55 billion recorded in the first half of 2010. Long-term UCITS attracted EUR 87 billion during the...
... percent during the quarter. Over the first half of 2012, total net assets of UCITS have increased by 5.6 percent. The number of UCITS at end June 2012 stood at 35,766, compared to 36,106 at ... debt crisis increased investor caution during the second quarter. This can be seen from the trends in monthly net sales1 for UCITS. Equity funds continued to suffer on account ofthe poor ... billion recorded in the previous quarter. This was mainly attributable to equity funds, which registered net outflows of EUR 28 billion in the second quarter. Balanced funds also registered a turnaround...
... between the projection ofthe tilt ofthe z axis on the xÂ,y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location ofthe rhombic (x, y) axes. The orientation ofthe ... that the Tyr is similarlyoriented with respect tothe iron and positioned to providethe H-bond tothe bound cyanide, and byimplication, tothe bound O2in the reduced form in each of the three ... case, the distance in bold isthe one in better agreement with the experiment, and each of the four distances dictate that the solution structure of WTmetHbCN is consistent only with the crystal...
... affected the level ofthe mRNAfor the other ADAM, confirming the specificity of theseASOs [19]. The ASOs reduced the level of their respectiveproteins in cell lysates but had little effect on the otherprotein ... failed to activate the shedding of APP, revealing that this is the first pharmacological agent to clearly differentiate between the shedding of APP and ACE. What is emerging fromthis and other ... indicating that the effect of APMA on ACE sheddingwas not as a result of over-expression ofthe protein.Together these data confirm that APMA induces the shedding of ACE but not the shedding of APP from...
... geographers about the aesthetic politics of landscape.There is no denying the importance ofthe visual.What is more, there is no escaping the inherently political quality ofthe visual; of imagery; ... into the distance. A sharp distinction was main- tained between the viewing subject and the viewedobject. The re-theorization of nature and dissolution of the subject–object dualism has led to ... Iprecisely wanted to highlight was the power of astory of landscape told with visual means to bringabout deep emotional feeling (which is not to betaken lightly, even if that is all too often the...
... from one side of the protein tothe other [1]. As the CCO is normally inserted in the mitochondrial membrane, this pumping, added to the consumption of protons in the mitochondrion, results in the formation ... other factors. None-theless, catalytic activity is still oneofthe few characteristicsthat reflect the actual role ofthe oxidase in the tissues.What is that role? The oxidase catalyzes the ... ascorbate. As the concen-tration of cytochrome c is increased, the fraction of cytochrome a that is reduced increases. The fraction of cytochrome a that is reduced is, in part, represented by the peak...
... easier to ask someone to do something on computer for them, instead of learning how to do it themselves. The third cause is lack of access to computer. The fourthcause is dissatisfactory school ... depend on other people.Causes The first cause ofthe problem of computer illiteracy isthe lack of useful information in the course of information science. The second cause is unwillingness to learning ... a oneofmain problems of business students.Symptoms The main symptom ofthe computer illiteracy is an inability in computer working. Forexample, student cannot find necessary information the...
... on the same parameter q. The order ofthe equation ofthe error is, usually, greater than theoneofthe differentialequation. Therefore, an higher number of conditions are needed to get the ... internal tothe boundary locus is the negative half complex plane. This completes the proof. L.Acetoetal. 11Proof. We consider only the case related tothe polynomial σk(z), being the other one ... When a discrete linear boundary value problem is considered, the error equation is well-conditioned if the number of initial conditions is equal to the number of roots ofthe char acteristic polynomial...
... wake at 6 a.m., but nap for two hours or so in the early afternoon. Thus the influence on one& apos;s sleep pattern is worthy of consideration when choosing an occupation. ...
... however, the inequality becomes even more pronounced. In families of 1 - 2 children, men maintain approximately the same number of hours of unpaid work as in childless households, but the number of ... work in the home rises to 52 per week, much of it, on doubt, due to childcare responsibilities. Interestingly, when there are three or more children in the household, men are found to work ... minutes on this task.model answer: The diagram reveals that the number of hours per week spent in unpaid work is unequally distributed between men and women. In households where there are no...
... the finished product, then adjustments will have to be made tothe product's design. Similarly, market research, which examines the extent and nature ofthe demand for products, has the role ... design is not only the result of product research, but is also influenced by testing and market research. If the testing stage (after assembly and inspection) reveals unacceptable problems in the ... the role of guiding product design to suit consumer demands which may change with time. Market research, while influenced by product sales, also serves to foster future sales by devising suitable...