of the gods of the gentiles and souls of the celestiall bodies and what places were consecrated in times past and to what deities
study on the immune response of orange spotted grouper (epinephelus coioides hamilton, 1822) cultured in khanh hoa province to bacteria vibrio parahaemolyticus
... new vaccine approach is highly demanded for development of marine fish farming The use of vaccines to avoid diseases and spread of pathogens also lead to reduced or no use of antibiotics and chemicals ... the farming industry 5 1.4.2 Vaccine for fish The use of commercial fish vaccines and experimental vaccine testing are conducted many places in the world Most vaccines are developed and tested ... antibodies in fish sera were analyzed by ELISA following Jakobsen et al (1999) The antibody response of orange-spotted grouper to the bacterin containing FIA and the protective efficacy testing:...
- 28
- 558
- 0
The effects of bottom up techniques in teaching listening skills to first year students at the university of fire fighting and prevention
... that they find it pleasing and interesting when they are listening With this type of listening, students are not reinforcing a structure or practicing a grammar point linked to the rest of the ... Types of listening There are many different types of listening We can classify these according to a number of variables, including listening purposes, the role of the listener and the types of text ... the material is available or not, the interest of the class and the teacher, the place in which the work is being carried out, the nature and the content of the listening text itself If one of...
- 46
- 1,172
- 4
Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing determined amounts of the purified UP12 were separated by SDS/PAGE along with the cell ... of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids long; they ... (5–10 lg of protein) were separated by SDS/PAGE using the standard Laemmli system [30] with 13% and 4% (w/w) acrylamide in the separating and stacking gels, respectively In order to estimate the ratio...
- 9
- 548
- 0
Báo cáo khoa học: Concepts and tools to exploit the potential of bacterial inclusion bodies in protein science and biotechnology pdf
... a-helices of the N-terminal 185 residues of the functional domain of the HA2 subunit of the in uenza virus hemagglutinin protein and to detect conformational heterogeneity of the protein within IBs ... proteins increases in the cell, chaperones are saturated and the equilibrium shifts toward the free version of r32, leading to induction of the stress response Nevertheless, the nature and variability ... those containing disulfide bonds or requiring cofactors, multidomain proteins, fusion proteins In the following we summarize recent progress in this field, whereas the use of IBs in the study of amyloid...
- 11
- 584
- 0
Báo cáo khoa học: Protein aggregation and amyloid fibril formation prediction software from primary sequence: towards controlling the formation of bacterial inclusion bodies pot
... chosen and the program calculates the average of a3v values over the sliding window and assigns it to the central residue of the window (sliding-window lengths of 5, 7, and 11 residues were trained ... contacts higher than the cut-off) and the size of the region is greater than or equal to the size of the sliding window used Optimum values for the cut-off (threshold) and the sliding-window length are ... are averaged inside the window and the average is assigned to the central residue of the window The ‘smoothed’ expected values for every position of the polypeptide chain provide the final profile,...
- 8
- 415
- 0
Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
... 422 and 428 aa; nine aa in total) were located near the interface of the physiological dimer The Cterminal domain may contain structural features involved in protein–protein interactions to form ... (disordered in the ligand-free state in YbgJ) In the absence of the ligand, the lid part is separated from the active site, which leads to a more open active site Upon the binding of ligand in the acyl-enzyme ... conditions: in the absence of the additives (referred to as N); in the presence of Tris (referred to as T); in the presence of l-glutamate (referred to as G); and in the presence of both Tris and l-glutamate...
- 11
- 521
- 0
Báo cáo khoa học: Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) pptx
... distribution and binding activities of FABPs and regulation of FABP genes, including FABP gene knock-out experiments [8], our understanding of the physiological function(s) of these proteins remains limited ... given a variety of names, such as ileal lipid-binding protein, intestinal 15 kDa protein, ileal bile acid-binding protein and gastrotropin, reflecting the speculations of authors on its intracellular ... region of the intestine (A) and remained confined to this region of the intestine up to days postfertilization (C), the last time point assayed (B) Distribution of fabp6 transcripts throughout the...
- 10
- 379
- 0
Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx
... CaM binding domains in the channel protein Indeed, we found two more sites, one in the C-terminus neighboring to the previously identified site and one domain in the N-terminus of hEAG1 The latter ... 1–206 in steps of 3) and C-terminal (amino-acids 480–962 in steps of and amino-acids 645–895 in steps of 2) cytosolic domains of hEAG1 Binding of BODIPY FL-labeled hCaM in the presence of 25 ... replacements of either the basic arginines or both phenylalanines (underlined in Fig 3C) in BD-C2 led to Ca2+ ⁄ CaM insensitivity of hEAG1 channels Ca2+-dependent binding of hCaM to the N-terminal binding...
- 13
- 500
- 0
Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... was incubated with different ratio of E600 In each case, the kinetics of inactivation of Rv1399c have been monitored by measuring the remaining activity, as a function of time, using tripropionin ... corresponding to pH values below 3.75 have been extrapolated due to Rv1399c instability at acidic pH (D) Rv1399c inhibitory effect using the E600 inhibitor as function of time The protein/inhibitor ... expression vector following the manufacturer’s instruction (Gateway, Invitrogen) The correct insertion of the Rv1399c ORF was confirmed by DNA sequencing In our hands, protein expression using the commercially...
- 9
- 584
- 0
Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt
... being minimal the trapping of two main-chain conformations and increased B-factors at 295 K suggests that the presence of the coordinating Lys in some way can in uence the dynamics of the ligand ... different In the wt structure the side chain slithers along the side of the protein surface, whereas in the M100K structure it points out into the solvent (Fig 4C) The position of the K99 side chain ... fold of the wt protein is maintained for the M100K variant, with an r.m.s.d for the ˚ backbone atoms of 0.4 A Significant deviations for main- and side-chain atoms in the ligand loop containing the...
- 15
- 509
- 0
Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt
... authors b-alanine) has been previously introduced in the sequence of the C-terminal heptapeptide of NKA, another peptide of the tachykinin family that binds the NK-1 and NK-2 receptors [HGly8] ... conformations of a-amino acids The atoms used in the rmsd calculation are those involved in the two peptide bonds and the two N- and C-terminal methyl groups, in order to compare the backbone ... Regarding the minor binding site NK-1m, SP and [Pro9]SP show a 10-fold increase in affinity compared to their affinity for the NK-1M binding site, with Ki in the subnanomolar range (0.13 nM) and...
- 11
- 860
- 0
The Carbon and Global Warming Potential Impacts of Organic Farming: Does It Have a Significant Role in an Energy Constrained World? pptx
... the growing of crops and livestock production, manufacture and application of agricultural inputs, processing, packaging, distribution and cold storage, preparing and serving, and disposing of ... carbon and to the type of organic matter stored and its permanence In some studies, soil C content increases within the top 7.5 cm of the soil profile, but results in no changes over the entire profile ... phase of the grain handling process—from harvest to threshing, drying, storage and milling—can produce up to 10% losses, for cumulative losses of 40% Fruit and vegetable losses run in the 10–70%...
- 41
- 524
- 1
The effect of grammar teaching (syntax) in English on 5 to 16 year olds’ accuracy and quality in written composition potx
... arising from the use of language in speech and writing on the other It is interesting to note that in the evaluation of the pilot of The Key Stage Strategy’ (Office for Standards in Education (Ofsted), ... of the study) to the review question (D) an overall weight taking into account (A), (B) and (C) The data were then synthesised to bring together the studies which answer the review question and ... including the contents of the databases, manuals, and keywording and dataextraction systems The Centre and authors give permission for users of the site to display and print the contents of the...
- 85
- 700
- 1
The Power of Habit: Why We Do What We Do in Life and Business
... behavior,” the doctor told her Everyone in the room felt like they were on the brink of something important And they were When you woke up this morning, what did you first? Did you hop in the shower, ... one point, we all consciously decided how much to eat and what to focus on when we got to the office, how often to have a drink or when to go for a jog Then we stopped making a choice, and the ... routines for building bases, setting strategic priorities, and de- ciding how to respond to attacks In those early days of the war, when the insurgency was spreading and death tolls were mounting,...
- 625
- 3,105
- 2
Introduction to Logic and to the Methodology of Deductive Sciences
... of learning, its domain of investigation embraces ever wider ranges of phenomena, and new theories continue to enter into the large circle of mathematical disciplines And finally, it is growing ... on Introduction to Logic was done by the present editor My thanks go, in the first place, to Professor Dana Scott, who took the initiative regarding the expansion and the revision of the original ... is entirely insignificant from the standpoint of requirements of other sciences, and of mathematics in particular In view of these circumstances, and of the aim which we have here, there will...
- 254
- 377
- 0
in what ways and to what effect did the british cinema of the second world war mobilize support for th
... guidelines were therefore enforced to raise the cultural and intellectual levels of the general public whilst also moderating the amount of information they were exposed to whether it be on the ... from the dangers of falling bombs Despite on screen warnings of impending air raids, many patrons chose to stay in their seats as they felt safer in the cinema than in their own homes due to the ... of the buildings The use of 'story' documentary was popular in portraying real situations, often using real people instead of actors, to reflect a nation united in her difficulties Although often...
- 4
- 433
- 0
Báo cáo khoa học: Salicylic acid and the hypersensitive response initiate distinct signal transduction pathways in tobacco that converge on the as-1-like element of the PR-1a promoter pot
... Plants handicapped in the accumulation of SA were infected with TMV, and PR-1 proteins were monitored in the necrotic tissue induced by the spreading lesions Whereas lesions and the accumulation of ... for expression in plants were integrated into the binary vector pBin19, and Agrobacterium-mediated transformation was employed to introduce gene constructs into the genome of tobacco (Nicotiana ... Gatz, C (2000) Tobacco transcription factor TGA2.2 is the main component of as-1binding factor ASF-1 and is involved in salicylic acid- and auxininducible expression of as-1-containing target promoters...
- 11
- 452
- 0
Báo cáo khoa học: The )148 to )124 region of c-jun interacts with a positive regulatory factor in rat liver and enhances transcription Dipali Sharma*, Sujata Ohri and Aparna Dixit ppt
... results in the dissociation of the dimers and the monomers are not able to bind to the )148 to )124 region of c-jun This study thus provides an insight into the molecular mechanisms regulating the ... (RLjunRP) in normal rat liver The positive regulatory factor interacting with this region was purified to homogeneity and the cDNA cloning of the gene encoding this factor is in progress to help in understanding ... even in the presence of 0.5 M NaCl indicated that the factor has a higher than usual affinity to the recognition sequence Most of the DNA-binding proteins exhibit binding activity with a rather...
- 9
- 449
- 0
Protecting the Elderly in Times of Disaster: The Critical Need for Comprehensive Disaster Planning and Exercise Design doc
... prior to, and during, a disaster Area Lack of adequate understanding and training of the first responder community concerning the special needs of these populations Area Lack of understanding of the ... Posed During Training Session There are several questions that are posed at the beginning our two day train -the- trainer sessions and conference intensives They are used to raise the awareness of issues ... together to go door -to- door to find these hidden residents, help them prepare, and to be able to reach them if needed during a disaster Issues addressed in this paper apply to these harder to...
- 7
- 405
- 0
Báo cáo khoa học: A distinct sequence in the adenine nucleotide translocase from Artemia franciscana embryos is associated with insensitivity to bongkrekate and atypical effects of adenine nucleotides on Ca2+ uptake and sequestration pdf
... in the presence and absence of the ANT inhibitory ligand cATR, and of the F0F1ATP synthase inhibitor oligomycin The results are shown in Fig In Fig 1A, ADP was present prior to the addition of ... [37] Mindful of (a) the well-established ligand profile of ANT, (b) the modulatory role of ANT in the mammalian PTP, and (c) the absence of a Ca2+-induced PTP in mitochondria from the embryos of ... exploiting the differential affinity of ADP and ATP for Mg2+ The rate of ATP appearing in the medium following addition of ADP to energized mitochondria (or vice versa in the case of deenergized mitochondria)...
- 15
- 505
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose