... comparison, the expression level of non-target genes ofthe same number was also correlated with the miRNA expression Density plot of correlation coefficient distribution was generated in R environment ... cells only, node C contained the genes upregulated in EB only, and node D contained the genes that were upregulated in adult cells HMVEC = human microvascular endothelial cells, HUVEC = human umbilical ... cellsThe expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in two types of adult cells HMVEC and HUVEC Differences in the expression...
... Increased frequencyof recombination in vitro can be misleading considering the inability to reconstitute the conditions of endogenous HIV-1 reverse transcription Compositions of buffers, concentrations ... oligonucleotide specifically onto the donor template Reverse transcription was started by the addition of HIV-1 RT at a final concentration of 400 nM and carried out for 60 Synthesis ofthe second ... in the presence or absence of HIV-1 nucleocapsid protein (NC) (schematic, Figure 2D) The addition of 5' non-homologous nt's to the 5' end ofthe donor prevents transfer from the end ofthe donor...
... Effect of heliquinomycin onthe RNA priming activity ofthe DNA polymerase-a/primase complex (A) Top: effect of increasing concentrations of heliquinomycin (HQ) onthe RNA priming action ofthe ... primer of approximately 10 nucleotides was synthesized only in the presence ofthe above complex The synthesis ofthe RNA primer was inhibited in the presence of heliquinomycin at an IC50 value of ... for the T antigen and an average ofthe values was plotted (B) Top: effects of increasing concentrations of heliquinomycin onthe DNA helicase activity ofthe Werner protein Bottom: proportion of...
... The Medicalization of Society This page intentionally left blank The Medicalization of Society Onthe Transformation ofHuman Conditions into Treatable Disorders peter conrad The Johns ... www.press.jhu.edu Library of Congress Cataloging-in-Publication Data Conrad, Peter, 1945– The medicalization of society : onthe transformation ofhuman conditions into treatable disorders / Peter Conrad p ; ... in investigating the rise of medicalization The focus was onthe creation (or construction) of new medical categories with the subsequent expansion of medical jurisdiction Concepts such as moral...
... the performance of VRLA cells Based onthe results presented herein, we can conclude the following Ž1 The XRD analyses showed that the major constituent ofthe additive Na SO4 in the cured plates ... work, we studied the effect of Na SO4 additive in the positive electrode onthe performance of VRLA cells for ES applications Experimental procedures 2.1 Cell construction Each cell contained two ... the paste was - wt.% The current density was controlled at mA cmy2 and the formation capacity had a theoretical capacity of about 200% under the formation After formation, the plates were washed...
... by the proximity ofthecells to the joint space and thebone Page 10 of 15 (page number not for citation purposes) respectively, alignment ofthe chondrocytes along the arcades of Benninghoff ... and CS4, one ofthe monomers ofthe polysaccharide portion of proteoglycan, showed significantly enhanced production of these cartilage matrix proteins at days 10 and 21 of culture in the aggregates ... development ofthe limbs, however, BMPs along with other regulators also mediate the replacement of chondrogenesis by endochondral ossification comprising chondrocyte maturation, hypertrophy, transition...
... expressed strongly in 60% and moderately in 40% ofthe normal tissues (Figure 4B) There was strong staining of FXR in the nucleus ofthe normal bile duct, which reflected the high expression of FXR ... expression (Figure 1C) of FXR Onthe contrary, treatment by the conjugated bile acids (GCA, GDCA and GCDCA) decreased the mRNA and protein expression of FXR According to density level, the mRNA ... Combined treatments of GS and CDCA could reverse the regulating effect of CDCA onthe expression of FXR Additionally, when treating cells with conjugated bile acids-GDCA, FXR expression was decreased...
... MFI of hBST-2 onthe surface of whole cells, and that in the square in the plot indicates MFI of hBST-2 onthe surface of p24-postive cellsThe amount of p24 in the culture supernatant (E), the ... percentage of p24-positive cells (F), the level of hBST-2 onthe surface of whole cells (G), and the level of hBST-2 onthe surface of p24-positive cells (H) following infection with either wild-type ... [7] Therefore, the relevance of surface hBST-2 down-regulation and the antagonistic action of Vpu onthe tethering ability of hBST-2 remain unclear We first set out to analyze the level of endogenous...
... cells include the bulk ofthecells that make up thehuman adult and each of these cells in their differentiated state has its own copy or copies ofthe genome with the only exception being cells ... classes of pluripotent stemcells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... somatic stemcells can be found in diverse tissues and organs The most common adult stemcells are thehematopoieticstem cells, mesechymal stemcells and neuronal stemcells Adult stemcells have...
... humans, 6–18% of CD4+ T cells constitutively express CD25 [11–17] Purified CD4+ CD25+ cells not proliferate in response to cross-linking of their TCRs They inhibit the activation of other T cells ... small number of CD25+ 243 Arthritis Research Vol No Horwitz et al cells in the naive fraction Although
... F, Avioli LV, Cheng SL: Regulation ofbone matrix protein expression and induction of differentiation ofhuman osteoblasts and humanbonemarrow stromal cells by bone morphogenetic protein-2 J ... all donors tested Another factor to consider is whether the age ofthe donors had an influence onthe results of this study Four donors were over 70 years old and one donor was aged 19 The relative ... Expression of BMP-2 and chordinstem cells during osteogenic differentiation ofhuman mesenchymal stemcells (a) Temporal progression ofbone morphogenetic protein (BMP)-2 and chordin expression during...
... activation of basal/ stemcells and their progression toward a metastatic status under the influence of Hh signalling remain to be further elucidated To further characterize the basal cells during ... and sham controls (Figure 3C; (a) indicated two small lymph nodes (on the left) from the sham controls and two enlarged lymph nodes (on the right) from the pCX-shh-IG-injected mice) The metastatic ... reconstituted in vivo and the effects of Hh overexpression onthe normal prostate stemcells was shown by the transformation of P63+/Patch1(Low or -) and P63+/Smo (Low or -) quiescent basal cells...
... differentiation of rat bone marrow- derived mesenchymal stemcells J Bone Miner Res (10):16061614 59 Matsubara T, Tsutsumi S, Pan H, et al 2004;A new technique to expand human mesenchymal stemcells ... 2007;Extracellular matrix made by bonemarrowcells facilitates expansion of marrow- derived mesenchymal progenitor cells and prevents their differentiation into osteoblasts J Bone Miner Res (12):1943-1956 ... mesenchymal stemcells from marrow Tissue Eng (4):415-428 129 Beresford JN, Graves SE, Smoothy CA 1993;Formation of mineralized nodules by bone derived cells in vitro: a model ofbone formation? Am J...
... (cancellous) bone Cortical bone forms the dense outer shell of most bones and it accounts for 80% ofthe total bone mass Cortical bone is mostly found in the shaft portion of long bones and the outer ... cancellous boneOnthe other hand, cancellous bone consists of a network of struts surrounding interconnected spaces It is the spongy interior bone tissue which makes up 20% ofthe total bone mass ... cancellous bones are different in that compact bone consists of haversian sites and osteons while cancellous bone does not Secondly, bone surrounds blood in compact bone while blood surrounds bone in the...
... the calcaneus bone As organs, tendons consist of three parts: the muscle attachment region, the substance ofthe tendon itself, and tendon to bone insertion region These three parts vary in their ... and the inorganic components Collagen constitutes approximately 90% ofthe total protein ofthe tendons or 65-75% ofthe dry mass of tendons Elastin accounts for only about % ofthe dry mass of ... tendons ofthe hand Following a full transection, there is a spontaneous retraction ofthe cut tendon ends In the Achilles tendon of both a rat and rabbit model, the gap formed by the retraction...
... residual bone graft particles were surrounded by a combination of newly formed bone and cartilage tissue The new bone formation in the defects ofthe CPCBB group was more prominent when compared to the ... with newly formed bone in the control groups which had received four week of HBOT (H&E×200) (Nbf; New bone formation, Bm; Bone marrow) Table 1: Overall results ofthebone histomorphometry and ... in host tissue – bone graft interactions The use of adjunctive HBOT to improve bone healing in autogenous bone transplants has been investigated in the histologic observation ofthe iliac grafts...
... The effect of consequent exposure of stress and dermal application of low doses of chlorpyrifos onthe expression of glial fibrillary acidic protein in the hippocampus of adult mice Journal of ... expression [17,18] The effect of dermal application of CPF on GFAP expression in the hippocampus has not been reported The aim of this study was to determine the expression of GFAP in the hippocampal ... Effect of concurrent application of heat, swim stress and repeated dermal application of chlorpyrifos onthe hippocampal neurons in mice Folia Neuropathol 2009, 47(1):60-68 Gordon CJ, Leon LR: Thermal...
... two-dimensional (2D) case Specifically, in OFDM system with symbol period T and subcarrier separation F, the transmission functions of OFDM system consist of translations and modulations of a single ... usually depends onthe precision of carrier frequency offset estimation and symbol timing A generalized framework for the prediction of OFDM system performance in the presence of carrier frequency offset ... channel dispersion point of view Synchronization is considered as a key factor of designing multicarrier modulation system receiver The synchronization precision significantly affects the receiver...
... satisfy these properties, then such an expression is only approximate To facilitate the application ofthe general FIR filter analysis of Section 3, let the signal s(n) pass through the transmission ... Reconstruction SNR for filterbank FDAF equalisation of constant delay Figure 8: Reconstruction SNR for filterbank FDAF equalisation ofthe linear delay channel The solid trace of Figure shows the ... applications, this may be the most appropriate measure of quality In others, including conventional equalisers and That is, the number of bins in thefrequency domain implementation is equal to the...
... IgG concentrations of CONT, CLA1, CLA2, Effects of Combination Dietary Conjugated Linoleic Acid with Vitamin A (Retinol) and Selenium onthe Response ofthe Immunoglobulin Production in Mice ... tion o f th e gu t lu m e n la va ge After blood collection, the end ofthe duodenum and the cranial part ofthe cecum were tied and both ends were cut One end ofthe small intestine was at the ... immunoglobulin concentrations than those of all the 0.5% CLA groups Even serum IgG concentration in 1.5% CLA group was decreased compare to that of control Over-dosage of CLA may affect the depression of...