nod scid gcnull mice support human cell engraftment from a variety of stem cell sources
báo cáo khoa học: "The transplant iron score as a predictor of stem cell transplant survival" pptx
... lysozyme, alpha-1 antitrypsin and other acute phase proteins Values for aspartate transaminase (AST), alanine transaminase (ALT), total bilirubin (TB), and the international normalized ratio (INR) ... represents a or AML indicates acute myeloid leukemia; ALL, acute lymphoblastic leukemia; MDS myelodysplastic syndrome; AA, aplastic anemia plant echocardiogram or MUGA scan All measures of EF were ... 7(3):195-212 Altes A, Remacha AF, Sarda P, Sancho FJ, Sureda A, Martino R, et al.: Frequent severe liver iron overload after stem cell transplantation and its possible association with invasive aspergillosis...
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Báo cáo y học: " Study protocol: The development of a pilot study employing a randomised controlled design to investigate the feasibility and effects of a peer support program following discharge from a specialist first-episode " pdf
... post-discharge We also aim to identify what participants want from a peer support program at this time If the program proves effective and acceptable to participants, we aim to develop a treatment manual ... below) and via a telephone call made by the RA to the new treating clinician asking about attendance at appointments and medication adherence These treatment adherence measures were created specifically ... participant will take place on-site with the case manager and the peer support coordinator present The nature of the intervention will be explained again and both the participant and PSW will sign a...
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Báo cáo y học: " Differential effects of cigarette smoke on oxidative stress and proinflammatory cytokine release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells" potx
... evidence of apoptosis in primary human small airway epithelial cells (SAEC) Primary human small airway epithelial cells (SAEC) were treated with media alone (control) and various concentrations of ... proinflammatory cytokine release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells Respir Res 2006, 7:132 Publish with Bio Med Central and every ... chromatin condensation, whereas necrotic or late apoptotic cells had normal/condensed nuclei that were brightly stained with ethidium bromide and appeared red Percentage of viable (white bars), apoptotic...
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Báo cáo y học: " Differential effects of cigarette smoke on oxidative stress and proinflammatory cytokine release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells" pps
... caused cytotoxicity in a variety of alveolar epithelial cells and in primary human small airway epithelial cells A Various alveolar epithelial cells such as human lung cancer cells (H1299), human ... evidence of apoptosis in human lung epithelial cell from papillary adenocarcinoma patient (H441) Human lung epithelial cell from papillary adenocarcinoma patient (H441) were treated with media alone ... study along with the primary human small airway epithelial cells (SAEC) The sources of various cell lines were as follows: the human adenocarcinoma epithelial cells (A5 49) derived from lungs of adenocarcinoma...
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Báo cáo sinh học: " Performance of AAV8 vectors expressing human factor IX from a hepatic-selective promoter following intravenous injection into rats" ppt
... additional limitations of AAV2 [10] A number of studies have already assessed the potential of AAV8 using mouse and non -human primate models for haemophilia gene therapy and application to muscular dystrophy ... (5'-TTTCCTGATGTGGACTATGT-3') and 200 nmol/L of anti-sense primer (5'-TCATGGAAGCCAGCACAGAACATG-3'), to quantify vector DNA by TaqMan PCR (Perkin-Elmer, Applied Biosystems, Foster City, California) A human FIX ... analysis of vector AAV8LP-1FIX into rats Results and Discussion Quantitative analysis of vector biodistribution following intravascular administration of AAV8LP-1FIX into rats DNA was extracted and...
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Báo cáo sinh học: " Spectratyping analysis of the islet-reactive T cell repertoire in diabetic NOD Igμnull mice after polyclonal B cell reconstitution" potx
... analysis of BV-BC of neonatal NOD. scid pancreas transplanted under the kidney capsule of diabetic NOD. Igμnull mice reconstituted with B cells (black bars) Spectratyping profile of pancreata from diabetic ... with anti-CD3/CD28 beads and 5-day supernatants were screened by cytokine bead assays (average and SD of animals) Vong et al Journal of Translational Medicine 2011, 9:101 http://www.translational-medicine.com/content/9/1/101 ... from whole pancreata, pancreatic lymph nodes and spleen and spectratyped according to the protocol of Pannetier et al [9] Total RNA was isolated from pancreatic tissue or cells isolated from spleen...
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o cáo hóa học:" Spectratyping analysis of the islet-reactive T cell repertoire in diabetic NOD Igμnull mice after polyclonal B cell reconstitution" docx
... analysis of BV-BC of neonatal NOD. scid pancreas transplanted under the kidney capsule of diabetic NOD. Igμnull mice reconstituted with B cells (black bars) Spectratyping profile of pancreata from diabetic ... with anti-CD3/CD28 beads and 5-day supernatants were screened by cytokine bead assays (average and SD of animals) Vong et al Journal of Translational Medicine 2011, 9:101 http://www.translational-medicine.com/content/9/1/101 ... from whole pancreata, pancreatic lymph nodes and spleen and spectratyped according to the protocol of Pannetier et al [9] Total RNA was isolated from pancreatic tissue or cells isolated from spleen...
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Báo cáo sinh học: " A strategy of tumor treatment in mice with doxorubicin-cyclophosphamide combination based on dendritic cell activation by human double-stranded DNA preparation" doc
... fragmented in an ultrasonic disintegrator at a frequency of 22 kHz to obtain a mixture of DNA fragments with a size 200-6,000 bp The human DNA was a pharmacopeian preparation “Panagen” (Registration ... study has demonstrated that a preparation of human fragmented dsDNA stimulated maturation of mouse DCs in culture [47] The salient finding was that the dsDNA preparation was just as effective at ... Takeshita F, Akira S, Ishii KJ: Molecular and cellular mechanisms of DNA vaccines Hum Vaccin 2008, 4:453-456 23 Martin DA, Elkon KB: Intracellular mammalian DNA stimulates myeloid dendritic cells...
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Improvements on humanized NOD SCID ll 2rg mice
... role at a much earlier stage in mouse macrophage development After the induction of human macrophages by human M-CSF, we observed engraftment of human macrophages in various organs, such as liver ... lymphomas around months old (Prochazka et al., 1992; Shultz et al., 1995) In addition, although the engraftment of human cells is much improved in NOD/ scid mice, high levels of human cell engraftment ... rest of the peritoneal lavage was centrifuged down to obtain cells for flow cytometry analysis, and to extract RNA for quantitative RT-PCR analysis as described above 2.15 Statistical analysis Data...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... Therapeutics (Tsukubu, Japan), and scrambled HO-2 siRNA was used as a negative control: HO-2 siRNA: sense, 5¢-AGGACUUCUUGAAAGGCAA CAUUAAAG-3¢, antisense, 3¢-UAUCCUGAAGAACUU UCCGUUGUAAUU-5¢; scrambled ... HO-2 siRNA: sense, 5¢-UAUAAGAGUCAGUACACAUCAUGGAAG-3¢, antisense, 3¢-UAAUAUUCUCAGUCAUGUGUAGUACCU-5¢ Another HO-2-specific siRNA, HO-2 siRNA1 (target base 212–232) reported by other investigators [32], ... cDNA were: forward, 5¢-AAGCTTCATGTCAGCGGAAGTG GAAAC-3¢; reverse, 5¢-CTGCAGTCACATGTAGTACC AGGCCAA-3¢ The sequence underlined is an artificial HindIII site A full-length HO-2 cDNA fragment was isolated...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... R, Takahashi K, Takeda K, Furuyama K, Kaneko K, Takahashi S, Tamai M & Shibahara S (2004) Expression of heme oxygenase-1 is repressed by interferon-gamma and induced by hypoxia in human retinal ... oxygenase by hemin in cultured pig alveolar macrophages Arch Biochem Biophys 188, 243–250 43 Okinaga S, Takahashi K, Takeda K, Yoshizawa M, Fujita H, Sasaki H & Shibahara S (1996) Regulation of human ... transcriptional regulation: TRANSFAC, TRRD and COMPEL Nucleic Acids Res 26, 362–367 47 Takahashi S, Takahashi Y, Ito K, Nagano T, Shibahara S & Miura T (1999) Positive and negative regulation of the human...
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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx
... LysoTracker incubation (B) Visualization of the Alexa-488 label (C) Visualization of acidic compartments by LysoTracker (D) Overlay of B and C, indicating co-localization of cystatin C and LysoTracker ... might be associated with its intracellular activity Nevertheless, a specific cystatin pathway implicating cellular uptake by active internalization of extracellularly located cystatins has not been ... MDA-MB-468, (all three cell lines are human breast adenocarcinoma), A- 431 (epidermoid carcinoma) and Capan-1 (human pancreas adenocarcinoma) Cell culture medium used was Dulbecco’s modified Eagle’s medium...
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Support to the identification of potential risks for the environment and human health arising from hydrocarbons operations involving hydraulic fracturing in Europe doc
... has issued Final Oil and Natural Gas Air Pollution Standards including for natural gas wells that are hydraulically fractured as well as Draft Permitting Guidance for Oil and Gas Hydraulic Fracturing ... research indicates that pathways may potentially exist in certain geological areas such as those encountered in parts of Pennsylvania, emphasising the need for a high standard of characterisation ... habitats and ecosystems from water degradation, reduced water quantity and quality; changes to water temperature; and erosion Areas already experiencing water scarcity may be affected especially...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... We also assessed their cytotoxicity against some human cell lines, such as human keratinocytes (HaCaT cells), human embryo lung fibroblast cells (HEL cells), human nasopharyngeal carcinoma cells ... NADH as well as cell viability Talanta 44, 1299–1305 Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura Y & Ueno K (1996) A combined assay of cell viability and in vitro cytotoxicity with a ... defined as an imbalance between cellular production of ROS and antioxidant defense mechanisms [1] It is a key component of inflammation and inflammatory disorders The processes associated with inflammatory...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... presence of EBV-encoded EBNA-1 protein are continuously propagated in 1% of initially transfected cells [4] EBV plasmid replication has been demonstrated for a large variety of human cell lines; ... transcription almost completely Transporter expression in 293 cells was assayed functionally by initial rates of uptake of substrates; initial rates of uptake are directly proportional to transporter ... Grundemann ¨ unpublished results) For pEBTet ⁄ SLC2 2A1 6h, the 5¢ interface is GGTACC CCCCCGGA; the 3¢ interface is ATGCCTGC GGGGATCCAC TAGTAACGGC CGCC AGTGTG CTGGAATTCT GCAGATATCC ATCACAC TGGCGGCC...
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Báo cáo khoa học: Poly(ADP-ribose) polymerase-1 protects excessive DNA strand breaks from deterioration during repair in human cell extracts pot
... is always an integral part of the BER process and that processing of uracil in human cell extracts may be divided into two major steps: removal of the damaged base and incision of the generated ... FEBS J L Parsons et al Role of PARP-1 in base excision repair A B Fig Increased degradation of repair intermediates in PARP-depleted cell extracts (A) HeLa WCE was incubated with a FAM-labelled ... England Biolabs (Beverly, MA, USA) Recombinant human PARP-1 was obtained from Alexis Biochemicals (Nottingham, UK) Antibodies XRCC1 (ab144) and DNA ligase III (ab587) antibodies were purchased from...
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human cell culture, volume iv
... CCA CAT ACG AGC AAG GCG TCG, 3: AAA ATG AAA GAA AAA GGA GAT ATT CCT GAG, 4: CTG AGG CCA CAT ATG AGA GTG GAT TTG TCA, 5: CAG AGA AAC AAA GGA AAC TTC CCT GGT CGA, 5b: TTC CCT AAC TAT AGC TCT GAG ... GGC AGA TGA CTC AGG GCT GCC CAA, 7: ATA AAT GAA AGT GTG CCA AGT CGC TTC TCA, 8: AAC GTT CCG ATA GAT GAT TCA GGG ATG CCC, 9: CAT TAT AAA TGA AAC AGT TCC AAA TCG CTT, 10: CTT ATT CAG AAA GCA GAA ATA ... ATA GCT GAA GGG TAC AGC GTC TCT CGG GAG, 20: TCT AAT ATT CAT CAA TGG CCA GCG ACC CT, : GCA GTA GAC GAT TCA CAG TT, 22: ATG CAG AGC GAT AAA GGA AG, 23: ATC TCA GAG AAG TCT GAA AT, 24: GAT TTT AAC...
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báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt
... display cellular characteristics of senescence J Vasc Surg 1998, 28:876-883 Hasan A, Murata H, Falabella A, Ochoa S, Zhou L, Badiavas E, Falanga V: Dermal fibroblasts from venous ulcers are unresponsive ... YlaHerttuala S, Alitalo K: Vascular endothelial growth factor-C accelerates diabetic wound healing Am J Pathol 2006, 169:1080-1087 Galiano RD, Tepper OM, Pelo CR, Bhatt KA, Callaghan M, Bastidas ... Lupattelli T, Morabito A: Early and five-year amputation and survival rate of diabetic patients with critical limb ischemia: data of a cohort study of 564 patients Eur J Vasc Endovasc Surg 2006,...
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báo cáo sinh học:" Paris on the Mekong: using the aid effectiveness agenda to support human resources for health in the Lao People''''s Democratic Republic" pdf
... resource constraints, historical patterns tend to be maintained According to a staff member of a multilateral agency: "PACSA does not have an overview of staffing allocation or technical awareness ... quantitative human resources analysis that informed the study and offered critical comments in the drafting and review of the manuscript All authors have read and approved the final manuscript Additional ... Total Source: Extracted from, Fernandes Antunes A, Khampasong T, Shuey D, Xaysida S, Vangkonvilay P, Manivong L, Ministry of Health of Lao People's Democratic Republic: Human Resources for Health:...
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
... purification and characterisation of virions, a major obstacle is contaminations of the viral preparations The contaminations can stem from soluble proteins derived from the producer cells or from ... Identification of cellular proteins associated with HHV- 6A Identification of cellular proteins associated with HHV- 6A particles Media from HHV- 6A- and mockinfected JJHAN cells were collected at dpi and ... analyses of the gradient peak fractions 11–15 showed apparently intact and spherical virus particles in the HHV- 6A sample Importantly, no obvious cellular material was visible in the peak fractions...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008