... MN participating in drafting the manuscript RR participating in drafting the manuscript and statistical analysis GF participating in statistical analysis and study design MRR: Preparing the revised ... embedded in paraffin, and finally, sections were stained with hematoxylin, eosin and tri-chrome gomori for light-microscopy studies, and were graded for ischemic fiber degeneration (IFD) and edema ... 1°C) and maintained on a 12 h light/dark cycle with free access to food and water All experiments were performed in accordance with institutional guidelines for animal care and use and also "Principles...
... ligand for β2-integrins, and α4-integrins (α4β1/VLA-4 and α4β1/VCAM-1), could be potential candidates [30-34] In addition, neutrophils also express CD11d/CD18 and α9-integrin, which both bind ... the ischemic and non-ischemic liver lobes were fixed in buffered 10% formalin, embedded in paraffin, and used for hematoxylin and eosin (H&E) staining Other portions of ischemic and non-ischemic ... and ischemia- reperfusioninjuryin rat liver Am J Physiol 1991, 260:G355 Jaeschke H, Hasegawa T: Role of neutrophils in acute inflammatory liver injury Liver Int 2006, 26:912-9 Lobb RR: Integrin-Immunoglobulin...
... result in vasopressin-inducing vasoconstriction and cardiac dysfunction in the injured heart Conclusions AVP infusion significantly depressed the myocardial function in I/R injured model and increased ... Vasopressin infusion results in higher mortality following I/R of the LAD than saline or dobutamine When compared with infusions of either saline or DOB, vasopressin results in dramatically increased ... baseline and day following I/R of the LAD Left ventricular tissue was dissected and flash frozen for protein extraction both in control (baseline) animals and at day following ischemia/ reperfusion...
... for estimating daily urine volume and measuring the ratio of urine protein to urine creatinine excretion Quantification of urine protein, BUN, and creatinine level was performed using standard laboratory ... and creatinine, urine amount, and the ratio of urine protein to creatinine Serum levels of blood urea nitrogen (BUN) and creatinine and the ratio of urine protein to creatinine in three groups ... Creatinine and BUN, Urine Amount and the Ratio of Urine Protein to Creatinine after IR Procedure Three time points (i.e 24 h, 72 h, and day 14 after the IR procedure) were chosen for determining...
... CK, CK-MB, cTnT and MDA, indicating that PEP-1-SOD1 and PEP-1-CAT cooperatively protected heart against ischemia- reperfusioninjury by removing OFR Ischemia- reperfusioninjury induces myocardial ... ischemia- reperfusioninjury To observe whether transduced PEP-1-SOD1 and PEP-1CAT protect myocardial ischemia- reperfusioninjuryin Page of 11 vivo, we established the model of myocardial ischemiareperfusion injury ... hypoxiareoxygenation injury [25,26] Our previous studies have shown that application of PEP-1-SOD1 or PEP-1-CAT can tranduced into myocardium and protected against myocardial ischemiareperfusion injuryin rats...
... blunt injury to the abdominal aorta J Trauma -Injury Inferction and Critical Care 2003, 55:795-799 Murry CE, Jennings RB, Reimer KA: Preconditioning with ischemia: a delay of lethal cell injuryin ... Discussion Nerve pathology in acute ischemic injury has beeen delineated in peripheral nerve andreperfusioninjury could amplify ischemic pathology Nerve ischemia plays a major role in the development ... Plexus and Peripheral Nerve Injury 2006, 1:2 with reperfusion [6] An aggrevated reperfusioninjuryin Streptozocine induced diabetic rats could be seen with less severe ischemic times [7] Clinical...
... of spinal cord injury: Pharmacologic management of spine injuries In Lin Spinal Cord Medicine Principles and Practice Edited by: W V, Cardenas DD Demos Medical Publishing LLC; 2003:114 Tsujino ... Chatzimichalis A, Fotopoulos V, Kokkinis K, Papadimitriou E, Tiniakos D, Marinos E, Bellenis I, Dougenis D: In Situ Cooling in a Lung Hilar Clamping Model of Ischemia- ReperfusionInjury Exp Biol Med (Maywood) ... receptor-activated and subsequently mediated pathways, initiates chain reactions and damage cellular macromolecule, including proteins, DNA and lipids, ultimately leading to cell death [6] Eighteen New Zealand...
... the zink finger protein A20 as it combines anti-apoptotic as well as anti-inflammatory effects [61] It has ubiquitinating and de-ubiquitinating properties, thus, activating and/ or inhibiting target ... Long-term effects of acute ischemiaandreperfusioninjury Kidney Int 2004, 66:523-527 Kosieradzki M, Rowinski W: Ischemia/ reperfusioninjuryin kidney transplantation: mechanisms and prevention Transplant ... is a serine-threonine protein kinase activated through the phosphatidylinositol 3-kinase (PI3-kinase) pathway counteracting apoptosis [67] Expression and activation of SGK1 are increased in various...
... the zink finger protein A20 as it combines anti-apoptotic as well as anti-inflammatory effects [61] It has ubiquitinating and de-ubiquitinating properties, thus, activating and/ or inhibiting target ... Long-term effects of acute ischemiaandreperfusioninjury Kidney Int 2004, 66:523-527 Kosieradzki M, Rowinski W: Ischemia/ reperfusioninjuryin kidney transplantation: mechanisms and prevention Transplant ... is a serine-threonine protein kinase activated through the phosphatidylinositol 3-kinase (PI3-kinase) pathway counteracting apoptosis [67] Expression and activation of SGK1 are increased in various...
... IR injury, thus proving that mast cells play a pivotal role in IR injury to skeletal muscle [5] Our IR injury model consists of 70 minutes tourniquet hind limb ischaemia followed by 24 h reperfusion ... fixed for 24 h in 10% BFS, washed in 0.1 M phosphate buffered saline (PBS) and processed into paraffin Five micron sections were cut, dewaxed and stained with Haematoxylin and Eosin (H&E) for general ... http://www.journal-inflammation.com/content/1/1/2 Figure Gastrocnemius muscle stained with Haematoxylin and Eosin Gastrocnemius muscle stained with Haematoxylin and Eosin Control (A & C) and IR treated hind...
... Medicine, Chennai, India They were fed on commercial rat chow (Hindustan Lever, India) and had free access to water Handling of the animals was approved by the Indian Ministry of Social Justices and ... extract was determined by administering the rats with Desmodium gangeticum orally for 30 days and then sacrificing them for reperfusion- induced ischemic injury Lipid peroxidation in drug treated ... root induces myocardial protection against ischemiareperfusioninjuryin isolated rat hearts, as indicated by the improved recovery of cardiac function, reduction in cardiac enzyme release in...
... (adenosine) results in an extraordinary cardioprotective effect during normothermic (37°C) ischaemia andreperfusion Khaliulin I et al Cardiovasc Res 2010;88:324-33 Initial rate of swelling A520.105 ... depletes glycogen in myocardium This promotes binding of Hexokinase II to the mitochondrial membrane and inhibition of mitochondria permeability transition pore (MPTP) opening Cyclic AMP-induced activation ... both PKA and Epac effectively protects heart against ishaemia /reperfusion injury PKA and Epac act synergicaly in the cardioprotective effect of cAMP Scheme of Signal Transduction of the cAMPInduced...
... cerebral ischemia/ reperfusioninjury [7] However, the role of dopamine D2 receptors on myocardial ischemia/ reperfusioninjury has not been clear In this study, we applied myocardial ischemia/ reperfusion ... or incubated in simulated ischemia solution and re-incubated in DMEM in the presence or absence of 10 μM bromocriptine or haloperidol, respectively (B) Changes in fluorescent intensities of intracellular ... chromatin, and mitochondria structure was intact in the control group Morphological change of apoptosis, including nuclear pycnosis, nuclear chromatin margination, aggregation and condensation, and...
... while still maintaining ischemia Atelectasis and reexpansion has been shown to induce injury involving edema, free radical generation, and apoptosis [33,34] This reexpansion-induced injury could ... of ischemia- reperfusioninjury of rat lung Am J Pathol 1997, 150:1773-1784 Eppinger MJ, Jones ML, Deeb GM, Bolling SF, Ward PA: Pattern of injuryand the role of neutrophils inreperfusioninjury ... populations including alveolar macrophages, type II epithelial cells and neutrophils in the progression of IR injury Our findings suggest that cytokines and chemokines, such as TNF-α, KC and MIP-2,...
... Hernandez-Lopez J, Rodriguez-Gallego C: Mannose-binding lectin and mannose-binding lectinassociated serine protease in susceptibility, severity, and outcome of pneumonia in adults J Allergy Clin ... pneumonia; CXCL2, chemokine CXC motif ligand 2; F5, coagulation factor V; IL-6, interleukin-6; IL-10, interleukin-10; MBL2, mannose-binding lectin-2; MIF, macrophage migration inhibitory factor; MV, ... determining susceptibility and outcome in ALI or ARDS still needs to grow and continue improving for the identification of true associations between genotype and clinical outcomes important in the...
... subjected to ischemia (30 min) /reperfusion (10 min), suggesting that like the NHE-1 inhibitor, NaHS may also protect cardiac myocytes against ischemia/ reperfusion- induced injury We further examined the ... oxide OxyHb Oxyhemoglobin PGE2 Prostaglandin E2 pHi Intracellular pH xii PI3K Phosphoinositol-3-kinase PKC Protein kinase C PKG Protein kinase G RAS Renin–angiotensin system RSH Thiol SO Sulfite ... protect hearts against ischemia/ reperfusion- induced injury (Karmazyn 2001), we further tested whether NaHS could also protect the cardiac myocytes against ischemia/ reperfusioninjury The experimental...