niche and the extrinsic factors in drosophila ovary
Interactions between early germ cells and inner germarial sheath cells in drosphila ovary
... the GSC niche together with the escort cells, produces extrinsic factors to regulate the behaviors of the stem cells and their progenies 1.2 GSC Niche Activities in Drosophila Ovary The GSC niche ... Microenvironment (Niche) and the Extrinsic Factors in Drosophila Ovary 11 1.2 GSC Niche Activities in Drosophila Ovary .12 1.2.1 Molecules Involved in GSC Niche Activities ... is then incorporated into the RNA-induced silencing complex (RISC), which binds to the complementary mRNA The binding of the RISC to the target mRNA can either promote the degradation of the...
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Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx
... receptors Dimeric secreted proteins such as noggin, chordin, follistatin and GDF3 bind and therby inactivate BMP dimers [16] Receptor heterooligomers induced by ligand binding signal via Smads, preformed ... can be efficiently bound and thereby inactivated by ID proteins In the rat tumour cell line AR42J, derived from the acinar pancreas, and isolated primary interferon-cNOD epithelial duct cells, ID2 ... signaling in stem cells tolerate lack of ALK3 in absence of the inhibitor Although functional proof is missing, the authors found up-regulation of ALK1 and ALK2 in these cells and suggest these...
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Báo cáo y học: "Global analysis of alternative splicing regulation by insulin and wingless signaling in Drosophila cells" ppt
... of insulin and wingless signaling pathways in Drosophila S2 cells Figure Activation of insulin and wingless signaling pathways in Drosophila S2 cells (a) Schematic representation of the insulin ... morphogens Wingless and Decapentaplegic ([56] and references therein) Another prominent example is the eye imaginal disc, the precursor of the eye Temporal coordination of inputs from the Hedgehog, Wingless, ... splicing of several key genes involved in wingless signaling, including the wingless receptor frizzled2 (fz2) [57] and the wingless modifier rotund (rn), were found [58] (Tables and 4) Equally interesting,...
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Báo cáo khóa học: ICAM-1 expression is highly NF-jB-dependent in A549 cells No role for ERK and p38 MAPK docx
... examine the role of the p38 and the ERK MAPK pathways, we examined the effects of the p38 MAPK inhibitor, SB203580, the MEK1 inhibitor, PD098059 and the MEK 1/2 inhibitor, U0126, on TNFa- and ... in pulmonary epithelial cells is currently lacking As NF-jB is considered to be a candidate for therapeutic intervention in airway in ammation, we have investigated the role of NF-jB in the induction ... ICAM-1 Taken together, these data highlight the potential utility of inhibiting the NF-jB signalling pathway in pulmonary in ammatory diseases and suggest that inhibitors of the p38 and ERK MAPK...
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Báo cáo khoa học: Mammalian 105 kDa heat shock family proteins suppress hydrogen peroxide-induced apoptosis through a p38 MAPK-dependent mitochondrial pathway in HeLa cells potx
... indirectly by cleaving Bid and inducing the release of cytochrome c from the mitochondria [46–49] In contrast, the ‘intrinsic’ pathway is activated directly by death stimuli, and induces the release ... signaling in HeLa cells treated with H2O2 Apoptosis signal-regulating kinases (ASKs) are serine ⁄ threonine kinases that activate both the p38 MAPK and JNK signaling pathways as MAPK kinase kinase ... these kinases was maintained for more than h However, the phosphorylation of JNK and p38 MAPK was markedly reduced in the cells overexpressing Hsp105a or Hsp105b Next, we examined whether the activation...
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Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
... 18SVH–linker and linker) 18VL, 18VH–linker and linker)18VL, 21SVH–linker and linker)21VL, 21VH–linker and linker)21VL and singlechain antibodies, scFvs, designated 18SHL, 18HL, 21SHL, and 21HL ... immunoglobulin and stabilizes and maintains the integrity of the immunoglobulin form [32,33] Bip contains the ER retention signal sequence KDEL (Lys-Asp-Glu-Leu) at the C-terminus and elaborates the ... at the C-terminus We compared the quantity of scFv intrabodies and assessed their binding activity to the WASP-EVH1 domain in the scFv gene-transfected T cells Finally, we succeeded in expressing...
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Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx
... proteins, have been described to promote the transfer of SUMO from E2 to target proteins in vivo The attachment of SUMO may result in diverse effects on the substrate protein, including cellular and ... several transcription factors [31] 426 LRH-1 is a target protein of SUMO modification [32,33] The hinge region lysine, residue 289 in the mouse and 224 in the human LRH-1, was the major SUMO conjugation ... associated with the targeting of LRH-1 into nuclear bodies that is inhibited by cAMP signaling Because the ovary expresses LRH-1, we further examined the effect of sumoylation site mutation on the nuclear...
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báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc
... that during the ovulation process the PKA-dependent pathway involving the ligand activation of G protein-coupled receptors by their ligands in cumulus and/ or granulosa cells induced the expression ... Competing interests The authors declare that they have no competing interests Authors' contributions YY and MS conceived of the study, participated in its design and coordination and drafted the ... expressed in porcine granulosa cells in vivo in response to hCG administration [12] Thus, in vivo during the ovulation process, LH induces EGF-like factor expression in granulosa cells and the release...
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Báo cáo sinh học: "Compound developmental eye disorders following inactivation of TGF signaling in neural-crest stem cells" doc
... corneal stroma and endothelium and to structures of the chamber angle at the junction between the cornea and the iris In mature eyes, the stroma of the iris, the ciliary body, and the trabecular ... (a) Toluidine blue staining of an adult eye The boxed areas correspond to (b) a detailed view of the corneal assembly, including outer epithelium, stroma, and inner endothelium, and (c) the chamber ... transcription factors in the periocular mesenchyme, the forkhead/winged-helix factor FOXC1 and the paired-like homeodomain factor PITX2 In humans, hypomorphic and overactivating mutations in either gene...
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Báo cáo y học: "Targeting Toll-like receptor signaling in plasmacytoid dendritic cells and autoreactive B cells as a therapy for lupus" pps
... of the multireceptor signaling complex containing TLR4 and other accessory molecules (for review, see [2,7]) The question remains regarding whether TLR activation can bypass tolerance and induce ... is that the inducibility or the function of SOCS proteins may be inadequate in autoimmune cells Finally, the endogenous supply of self TLR ligands may be increased either because of the abnormal ... immunoglobulin switch regions) These motifs can block TLR9-induced activation in both a cis and trans manner Surprisingly, chromatin-containing immune complexes in lupus sera were capable of inducing...
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Endofin is a novel component in EGR EGFR oncogenic signaling
... affect the intensity of MAPK signaling 1.8 FYVE domain-containing proteins A group of proteins that are also implicated in EGFR trafficking and signaling are the FYVE domain-containing proteins These ... FYVE domain proteins is EEA1 It binds to PI3P via its FYVE domain in the C-terminus and interacts with Rab5 at two binding sites, one adjacent to the FYVE domain and the other at the N-terminus (Christoforidis ... Endosomal-specific signaling 35 FYVE domain-containing proteins 36 1.8.1 The FYVE zinc-finger domain 36 1.8.2 FYVE domain proteins and membrane trafficking 37 1.8.3 FYVE domain proteins and signal transduction...
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The study of interactions of transmembrane receptors and intracellular signaling proteins in live cells by fluorescence correlation and cross correlation spectroscopy
... C-terminus domain, to expose binding sites for Src-homology-2 (SH2) domain-containing proteins and phosphotyrosine-binding (PTB) domain-containing proteins Adaptor proteins with a SH2-binding domain such ... ligands bind to the extracellular domains of two receptor subunits and form a 2:2 complex (42), resulting in activation of the intracellular kinases of the receptors Upon ligand binding, the ... epitope tag heparin-binding epidermal growth factor protein kinase C-related kinase homology region IQ motif containing GTPase activating protein infrared insulin receptor tyrosine kinase substrate...
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Dysfunctional signaling pathway for nitric oxide production in endothelial cells chronically exposed to high glucose or high fatty acids
... between these factors results in a variable deterioration in insulin sensitivity and insulin secretion 1.1.2 Diabetes-related vascular complications The discovery of insulin in 1921 led to the belief ... for 18 Introduction 69% of the basal norepinephrine release 41 in the control of vascular tone, and thus received the most interest in endothelial dysfunction studies In addition to inducing vascular ... function by acting at the level of the plasma membrane or by increasing the flux and concentration of glucose inside the cell, but the underlying mechanisms remain incompletely understood There are...
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Expression of neurogranin tagged with enhanced green fluorescence protein in HEK293 cells and its effects on neuronal signaling
... region and those coding for the N-terminal amino acid; the second contains the remaining 73 amino acids and a short tail of 3’-untranslated region and the third and the fourth contain the remaining ... regulation in rats and in humans -2- 1.2 Calpacitin family and its members Because of the conserved calmodulin-binding domain and thereby the abilities to regulate free calmodulin availability, the ... protein kinase C substrate Rat Ng cDNA codes for a 78 amino acid protein which contains a conserved IQ motif that defines the overlapping region of CaM binding domain and PKC recoginition domain...
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Expression of neurogranin tagged with enhanced green fluorescence protein in HEK293 cells and its effects on neuronal signaling
... region and those coding for the N-terminal amino acid; the second contains the remaining 73 amino acids and a short tail of 3’-untranslated region and the third and the fourth contain the remaining ... regulation in rats and in humans -2- 1.2 Calpacitin family and its members Because of the conserved calmodulin-binding domain and thereby the abilities to regulate free calmodulin availability, the ... protein kinase C substrate Rat Ng cDNA codes for a 78 amino acid protein which contains a conserved IQ motif that defines the overlapping region of CaM binding domain and PKC recoginition domain...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
... with continuous shaking E coli were maintained in the LB liquid medium (containing 100 µg/ml ampicillin) at 37°C for 12 h with continuous shaking Preparation of Hp and E.coli extract The Hp and E ... containing μmol/L DHR-123 and 60 μg/ml, 120 μg/ml, 240 μg/ml, 480 μg/ml or 960 μg/ml Hp extract for 24 h and those in the other group maintained in the medium containing 480 μg/ml Hp extract and ... et al., their results showed Hp infection resulted in increased mutations in the non-coding D-loop as well as the coding genes ND1 and COI of mtDNA of gastric cells [12] The increase in the number...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... altered expression of many proteins involved in apoptosis, including the expression profiles of proteins involved in the mitochondrial (bcl-2, bax, and bcl-x, etc.) and stress reaction-related pathways ... almost no increase in the phosphorylated JNKs protein level in the cell line Twenty-four-hour exposure to hypoxia resulted in a Int J Med Sci 2007, 42 marked increase (1.8-fold of control) in the level ... 24hr exposure to hypoxia On the other hand, the protein expression of caspase 3, PTEN, and JNK1 in JAR cells exhibited a 4.96-fold increase, 1.92-fold increase, and in the level of phosphorylated...
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Báo cáo y học: " Low concentration of ethanol induce apoptosis in HepG2 cells: role of various signal transduction pathways"
... shifts the balance into the apoptotic direction after its initial effect on protein kinases phosphorylation The most strikingly and novel finding obtained hereby was the strong inhibition of the ... consider in the pathophysiology of ethanol-induced effects on HepG2 cells In conclusion, the data obtained in the present study support our working hypothesis and confirm a balance between pro- and ... representing increases of 1- and 12-fold, respectively These data confirm the effect of ethanol on intracellular signal transduction resulting in increased expression of transcription factors Int...
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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
... approximately 101 bp and 444 bp in size in samples either containing the heterozygous C/T or homozygous T/T SNP while these fragments could not be detected in samples lacking the SNP and therefore corresponded ... wild type samples containing the newly identified C/T SNP versus the reference sequence (Ref Seq.) and samples lacking the SNP Int J Med Sci 2006, Direct DNA sequencing and mutational analysis ... 95°C for minutes and subsequently re-hybridized In case of sequence deviations in the wildtype samples from the reference sequence this resulted in the formation of heteroduplexes containing mismatches...
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Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
... for claudin-1 (17) This opens up the possibility that Wnt signaling is involved in claudin-1 expression and localization 99 Indeed, a study shows that increasing β-catenin increases the expression ... compromised skin integrity, and this has been shown to correlate to the lack of claudin-1 and also to the loss of p63 (15) Further, we have previously shown that increasing claudin-1 expression increases ... using bryostatin instead increased the expression of claudin-1 in the tight junction complex, and increased tight junction barrier integrity (9) In melanoma, we have shown that claudin-1 expression...
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