... acids and agar were from BD (Franklin Lakes, NJ, USA) Ammonium sulphate and amino acids were from Merck (Darmstadt, Germany) Strains, plasmids and primers The strains and plasmids used are listed ... sequencing and by growing on the selective media, SC-Ura-Leu and SC-Leu-His GB1 cells transformed with empty pCBG1202 vector and the same vector containing wild-type eEF2 were used as negative and positive ... suggest that threonines T56 and T58 arenot essential for the viability of yeast cells under normal growth conditions Both threonines could be replaced by amino acids that cannot be phosphorylated...
... specific mutation andnot to some unpredictable side effects Function of hypA and hypB in hydrogenase maturation The function of hypA and hypB was investigated in more detail Because HypA and HypB were ... and urease activity and metal transport Surprisingly, DhupE did not show a difference compared with wild-type cells concerning its hydrogenase activity (Fig 1A) or its urease activity (data not ... hypB1 and sll1079 hypB2 The only hyp genes encoded in the same Cyanobacterial hydrogenase accessory genes gene cluster are hypA2 and hypB2 A similarity search in the complete genome did not uncover...
... survival and recurrence when we computed the Kaplan-Meier estimates and compared differences in survival based on genotypes using a log rank test (data not shown) Page of (page number not for ... three individual genotypes, and appear in bold font if ≤ 0.05 The significance was lost after adjustment for age, sex, phenotypic index, moles, freckles, and race £Genotypes 5A6A and 6A6A combined ... growth and migration as well as angiogenesis [1] MMPs are expressed in melanocytes and their overexpression has been linked to tumor development, progression and metastasis [2-5] Certain MMPs are...
... Castillo and coworkers [1] - and prospective trials comparing different extracorporeal circuit management strategies and providing recommendations to operators in the field are welcome and urgently ... session length, downtime and dose are progressively leading to more detailed research in the field of anticoagulaPage of (page number not for citation purposes) tion and vascular access In the ... is not the most important difference between adults and children Rather, practical clinical problems have significant impact in pediatric CRRT, such as the frequent interruption of total parenteral...
... 12:00 PM 3:00 PM 6:00 PM 9:00 PM 12:00 AM 3:00 AM 6:00 AM 9:00 AM SB50 Brands LEADING BRANDS 328,091 Super Bowl 50 Brand mentions over the last 24 hours • Doritos claimed 33.5% of the Super Bowl ... SENTIMENT Game • Ads The words “best”, “happy”, “congrats”, and “great” were the most frequently used terms among all positive comments Gender and Age Demographics Game Ads Game Ads • In the week leading ... during the game resulting in an equal split between males & females • The majority of tweets were shared by individuals in the 25-44 age range ...
... kids are plugged in to social media, only about 40% of parents are involved themselves [4] Parents may not be aware of their children’s “pages” and don’t fully understand privacy policies and ... effects of social media on our children, why then, are many parents not taking more action to protect their children? There perhaps are three major reasons: 1) Parents are unaware of the issues ... e ouur opinion and d share it with friends and peers p [3] Thiss world off social media gives scope to everyone to t express and d share t be the partt of it ideeas, thoughts and feelings, who...
... forge meaningful, profitable and long-lasting connections with their customers through email marketing and social media Featuring a unique combination of technology and services, StrongMail takes ... provides you with easy access to customer data, superior performance, low total cost of ownership and award-winning agency services Learn more at www.strongmail.com or call us at (800) 971-0380...
... involvement in home life, interactions with family members and peers and the development and enactment of cognitive, physical and social skills and compliance with rules [15] Children’s social functioning ... peer-problems and self-reported PA, a positive association between pro-social behaviour and PA among 11-12 year old boys and girls and a positive association between conduct problems and PA among ... acceptance scores were moderate to high and did not differ significantly between boys and girls Boys engaged in significantly greater PA (CPM and MVPA) daily and after school than girls Boys performed...
... social mediaand integrate their social media activities • Social media marketing takes a lot of time: The majority of marketers (58%) are using social media for hours or more each week, and more ... employing social media marketing for 12 months or less, Facebook and Twitter are their top two choices Tools used by those using social media for to years Facebook and Twitter are the two standouts among ... analytics are the top three areas that social media marketers are outsourcing Those with or more years experience are more likely to outsource design and development tasks (32%) 2011 Social Media...
... social media tools? What social media platforms are customers going to be looking at in three months and three years from now? Which elements are persistent and long-term versus those that are fads? ... social media tools: Wondering which social media tools marketers are using the most? Those questions are answered, along with an examination of what the most experienced folks are using • Social media ... generates sales and what generates business? How I monetize social media marketing? #9: How I cohesively tie different social media efforts together? Can social mediaand traditional media work together?...
... social mediaand branding, brand and branding in general and branding in fast moving consumer goods sector, brand strategy, brand equity and brand awareness, the management of brand portfolio The area ... brand strategy, brand equity and assets that underlie brand equity which are brand awareness, perceived quality, brand loyalty, brand association and other proprietary brand assets 1.1.2.3 B RAND ... awareness? How is brand awareness measured when traditional mediaare used? How is brand awareness measured when social mediaare used? What is measure regarding brand awareness and social mediaThe number...
... anti-a, -b, -c, -d, -e, and -f for immunoblotting were obtained from Gibco (Grand Island, NY, USA) Rabbit anti-PKCf for immunoprecipitation and activity determination, and the antiPKCf blocking ... rapidly aspirating the medium and immersing the dishes in liquid N2 Glycolysis and glycogen synthesis Materials and methods Materials Enzymes, M199 medium, collagenase A and the transfection agent ... fluoride, 20 mM 2-mercaptoethanol) and centrifuged at 100 000 g for 30 Supernatant and membrane fraction were diluted with lysis buffer (without sucrose and EGTA) and 8–14 lg of protein from each...
... Ni(II) and Cu(II) complexes of peptides at I = 0.1 M (KNO3) and 25 C Standard deviations on the last digits are given in parentheses EPR The X-band EPR spectra of Cu(II) complexes of VIHN and DAHKam ... considered Comparison of Cu(II) and Ni(II) binding between model peptides and albumins Afnity for Ni(II) at site I can be compared between albumins on one hand and DAHam and DAHKam on the other Much ... Tables and thus present only the novel data: protonation constants for DAHKam and VIHN, and stability constants (log a values) of Cu(II)-VIHN, Cu-DAHKam and Ni-DAHKam systems The parameters of CD and...
... activity and statistical significances are expressed as indicated in (A.) HsSRF, DmSRF and AfSRF and the endogenous factor in C2C12 cells These data also show similar levels of expression of HsSRF and ... conserved MADS box and immediately C-terminal SAM domain, devoid of the rest of N- and C-terminal regions, did not activate transcription, suggesting that this region is necessary but not sufficient ... the N-terminal and MADS box regions from A franciscana SRF EXPERIMENTAL PROCEDURES Cell culture and transfection Myoblastoid C2C12 [24] and monkey kidney epithelial cells, Bsc1 [25] and Bsc40 (a...
... good ideas, and the best ones attract large numbers of users Users arenot prepared to pay for services even if they are useful Services are assumed to be free Free basic services are a good lure ... http://www.ischool.berkeley.edu/~plyman/articles/Lazerow.pdf 12 users of social media may include individuals who not belong to the community and only utilise social media Social media applications are characterised by9: • participation ... a synonym for social media, clearly raising the role of users and also the fact that the applications arenot related solely to the media sector It is essential for social media applications that...
... users and those who have a cell phone but no landline are less likely to get local TV news on a typical day than non-internet users and those who have a landline phone Political Independents are ... compared with 81% of Democrats and 82% of Republicans National broadcast and cable TV news: These are some of the demographic groups that are particularly likely to watch national broadcast and ... (those who have dropped their landline and rely exclusively on their cell phone) get radio news on a typical day, compared with 39% of those who rely exclusively on landlines Radio news is also...
... Raghavan, and Andrew Tomkins 2004 Structure and evolution of blogspace Commun ACM, 47:35–39, December Amanda Lenhart, Kristen Purcell, Aaron Smith, and Kathryn Zickuhr 2010 Social mediaand young ... AIM and weblogs coincide with the earlier shifts at 1977 and 1979, SMS messaging coincide with both the earlier and later shifts at 1979 and 1982, and the social networking sites, MySpace and ... in the profile page from 1500 random blogs in three age groups These age groups are used solely to illustrate the differences that occur at different ages andarenot used in our classification experiments...
... incorporate social media into their marketing and communication strategies, initiatives, and campaigns? What are their goals for social media? Which social media sites and tools are in use in the ... education sector and which are most successful? What are current best practices? How they staff their social media efforts? How are schools measuring the ROI or impact of social media initiatives? ... input from us Another unit manages all of our social media activities, with input from us We handle our own social media activities, with some input from others We handle our own social media activities,...
... Healthcare Research and Quality Centers for Disease Control and Prevention Centers for Medicare & Medicaid Services Early and Periodic Screening, Diagnostic, and Treatment Department of Health and ... dental disease and receive dental care, arenot new Our reports dating back to 2000 highlighted the problem of chronic dental disease and the factors that contribute to low use of dental care by lowincome ... dental disease and often not receive needed dental care, and although receipt of dental care has improved somewhat in recent years, the extent of dental disease for most age groups has not Information...