movements home ranges activity and dispersal
Tài liệu Carnivore Ecology and Conservation A Handbook of Techniques pptx
... thoughts Movements, home ranges, activity, and dispersal Roger A Powell 9.1 Research design 9.2 Movements 9.3 Home range 9.4 Territories 9.5 Estimating animals home- ranges and territories 9.6 Home- range ... gulo) and far northern bears (polar, brown, and black) and wolves with home ranges of 100s to >1000s of km2 And, while black-footed ferrets are recovering from a population low of 10 individuals and ... researchers and managers must understand the concepts pertinent to their goals before they can design research In this chapter, I (R.A Powell) dene terms and concepts related to movements and home ranges...
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Carnivore Ecology and Conservation: A Handbook of Techniques pptx
... thoughts Movements, home ranges, activity, and dispersal Roger A Powell 9.1 Research design 9.2 Movements 9.3 Home range 9.4 Territories 9.5 Estimating animals home- ranges and territories 9.6 Home- range ... gulo) and far northern bears (polar, brown, and black) and wolves with home ranges of 100s to >1000s of km2 And, while black-footed ferrets are recovering from a population low of 10 individuals and ... researchers and managers must understand the concepts pertinent to their goals before they can design research In this chapter, I (R.A Powell) dene terms and concepts related to movements and home ranges...
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Báo cáo y học: "Pravastatin Provides Antioxidant Activity and Protection of Erythrocytes Loaded Primaqe"
... fragility and oxidative damage of erythrocytes of zinc-deficient rats J Nutr 1997; 127: 1290–1296 Lajos N, Miki N, Sandor S Protein and non-protein sulfhydryls and disulfides in gastric mucosa and ... TCA and resuspended in 1.0 ml of M HCl for blank and M HCl containing 2% 2,4dinitrophenyl hydrazine After incubation for h at 37°C, protein samples were washed with alcohol and ethyl acetate, and ... gluteraldehyde Aldehyde was drained and rinsed times each of in phosphate buffer and post-fixed in osmium tetroxide for h After this, the samples were rinsed in distilled water and then dehydrated using...
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Windows Home Server Protect and Simplify Your Digital Life
... Chapter 13 Security and Your Home Server 235 How Windows Home Server Helps with Security 235 Monitoring and Managing Home Network Health 236 Understanding Security ... backups and user accounts on Windows Home Server, and gain an understanding of how specific facets of your home network might drive you to change some of the default behaviors of Windows Home Server ... differential, and full backups Windows Home Server presents backup and restore functionality in a manner that provides a sensible and usable default experience, and also offers flexibility to handle...
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Tài liệu Physical Activity and Women’s Health pptx
... occupational and leisuretime activity was combined and subjects were grouped into three activity classifications A strong inverse relationship was found between physical activity and CHD mortality ... observational and experimental evidence that physical inactivity plays a significant role in the development of cardiovascular disease in women, and that habitual physical activity and at least ... based programs in schools and communities that are culturally sensitive and ethnic-specific R EF ER EN CE S Albanes, D., Blair, A., and Taylor, P.R (1989) Physical activity and risk of cancer in...
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Tài liệu Time trends in leisure time physical activity and physical fitness in elderly people: 20 year followup of the Spanish population national health survey (1987-2006) docx
... proportional random selection of primary and secondary sampling units (towns and sections, respectively), with the final units (individuals) being selected by means of random routes and sex- and age-based ... sleeping > hours per day and those sleeping < hours per day Statistical analysis In this study we analyzed physical activity and physical fitness separately for men and women and we excluded respondents ... Team: Older adults, chronic disease and leisuretime physical activity Gerontology 2009, 55:64-72 Centers for Disease Control and Prevention: Physical activity and health A report of the Surgeon...
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Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
... = 9.76 · 10)10 M and AzU = 50.8 · 10)10 M and for the wild-type XAN, X6U = 1.02 · 10)10 M, XyU = 7.72 · 10)10 M and AzU = 9.65 · 10)10 M for the activity on X6, Xylazyme AX and Azo-wheat AX, ... single curve) The reported temperature and pH ranges indicate the intervals in which the observed activity was at least 95% of the maximal activity of the enzyme Activity (%) on Xylazyme AX Xylohexaose ... relative to the ratio of the wild-type enzyme (100%) and were calculated based on two independent activity measurements on X6 and two independent activity measurements on Xylazyme AX on the same...
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Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
... 38-kDa and 19-kDa proteins were mixed and subjected to nondenaturing PAGE, these proteins comigrated with each other, showing a broad protein band that differed from the respective original bands and ... time-dependent enhancement of the 57-kDa band with a concomitant diminution of the 38-kDa and 19-kDa bands, as analyzed by SDS ⁄ PAGE (Fig 3A) The 38-kDa and 19-kDa bands did not disappear completely ... wild-type 37-kDa mature form were incubated at pH and 50 °C for 10 and then subjected to SDS ⁄ PAGE, the 19-kDa band disappeared, whereas the 37-kDa band remained (Fig 2C) During the course of our...
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Tài liệu Báo cáo khoa học:Symmetric fluoro-substituted diol-based HIV protease inhibitors Ortho-fluorinated and meta-fluorinated P1/P1¢-benzyloxy side groups significantly improve the antiviral activity and preserve binding efficacyy docx
... Padgett, W.L., Oshunleti, O., Daly, J.W & Kirk, K.L (2000) Syntheses of (R )and (S)-2- and 6-fluoronorepinephrine and (R)- and (S)-2- and 6-fluoroepinephrine: effect of stereochemistry on fluorine-induced ... and Leu23, which are displaced ˚ 0.5 A with respect to inhibitors 1–5 2- and 3-Fluorobenzyloxy side groups The mono-substituted inhibitors and are fluoro-substituted in the ortho (2-fluoro) and ... extends the contact surface and has polarity differences This is documented by the threefold improvement in activity of inhibitors and against the triple mutant M46I, I82V and V84A compared with the...
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... behaviour and a low unstable inhibitory activity, and for I137A a biphasic loss of activity (Fig and Table 3) The phenotype of these substitutions is therefore different from that of N139A, T146A and ... with an A leads to substrate behaviour and a low, stable inhibitory activity (Fig and Table 3) This W is located in the cleft between hF and s2A (Fig 5), and the presence of an aromatic side chain ... 100 and 128 are highly conserved among serpins (F119 and F147, respectively, in a1PI) [2] and buried in the hydrophobic interface between hF and b-sheet A (Fig 5) The substrate behaviour and...
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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
... intense signals of 70 and 50 kDa, while the bands at 60 and 150 kDa vanished With oxyblot (Fig 4C), antibodies stained carbonylated proteins mainly in band ranges of 30–60 and 70–120 kDa at 10 ... exhibited a comparable pattern of bands, although two bands of 60 and 150 kDa strongly visible at 10 months (lane 1) were absent at 27 months, and an important band of 70 kDa (lane 2) emerged in ... purity ATP-stimulated protease activity in aging rats Fig Lon protease quantification and activity in liver mitochondrial matrix from 10- and 27-month-old rats Activity was determined in the absence...
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Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf
... Kdo, and tetrasaccharide 1-monophosphate (TS 1-MP) One-dimensional 1H- and 31P-, and two-dimensional homo- (1H,1H-DQF-COSY) and heteronuclear (1H,13C-,1H,31P-HMQC) NMR spectroscopy (Figs and 6) and ... C18:0, C20:0 and 3-OH-C20:0 MALDI-MS and ESI-MS analysis As a first step in the structural analysis, LPS and de-Oacylated LPS of C trachomatis serotype E was analyzed by MALDI-TOF and ESI-FT-ICR ... from C trachomatis serotype E and L2 were of similar activity and both less active than smooth LPS by a factor of 100 whereas, LPS from Chl psittaci was of lower activity than the two other chlamydial...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
... on their effects on stability and activity parameters of RNase-A Functional stability and activity by osmolytes Results and Discussion Protein stability and enzyme activity have a well-correlated ... advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the relationship between protein stabilization by osmolytes and its consequent ... presence and absence of almost all naturally occurring osmolytes We report here that protein stability and activity are not largely coupled in the presence of osmolytes However, protein stability and...
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Báo cáo khoa học: Haptoglobin binds the antiatherogenic protein apolipoprotein E – impairment of apolipoprotein E stimulation of both lecithin:cholesterol acyltransferase activity and cholesterol uptake by hepatocytes pdf
... polyacrylamide gel in denaturing and reducing conditions, and by western blotting (A) Coomassie-stained bands of isolated Hpt (lane 1), standard ApoE (lane 2), standard ApoA-I (lane 3), and partially purified ... HRP-conjugated avidin and ECL Coomassiestained bands of VLDL and LDL proteins are shown in lanes and 2, respectively VLDL and LDL proteins, blotted onto the PVDF membrane and incubated with biotinylated ... anti-Hpt IgG and rabbit anti-goat HRP-conjugated IgG Two bands, reacting with the antibodies and with the molecular masses of b and a2 (41 and 21 kDa, respectively), were detected (Fig 4, lanes and 6)...
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Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt
... mutations from both the stability and activity mutants, and demonstrated a significant improvement in both stability and catalytic activity (Fig 1) As such, the activity and stability mutation combinations ... remaining activity, mutants AS1–AS7 were selected on the basis of a co-improvement in stability and activity For example, the T1 ⁄ values for AS4 and AS6 were 6.7 and 11.2 °C higher, respectively, and ... wild-type protein and two mutants, AS4 and AS7, were purified to homogeneity with purification yields of over 40%, and investigated for their stability and activity at temperatures between 40 and 80 °C...
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Báo cáo khoa học: Hyperthermophilic enzymes ) stability, activity and implementation strategies for high temperature applications pot
... food and drug biosynthesis applications; (g) enzyme immobilization may increase heat stability and therefore, improve biocatalyst performance; and (h) protein engineering by rational design and ... hydrophilicities and charges at given environmental conditions, such as temperature, concentration, ionic strength and pH Thus, the diverse chemical and physical properties of proteins and surfaces ... (a) 30 000 and 600-fold increases in half-life compared to free and single-point attached enzymes, respectively; (b) retention of 65% of residual activity (cf soluble) upon bonding; and (c) retention...
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Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf
... ATPase domain and C-terminal helix [36] showing the location of residues that were mutated The TRP, RDF and DEL sequences are shown in green, dark blue and red, respectively The b domain and C-terminal ... as well as the b-domain DEL sequence, for Vps4p ATPase activity, wild-type and mutant Vps4p proteins were purified (Fig 4A) and the ATPase activity of each Vps4p protein was assayed (Fig 4B) Mutant ... SDS ⁄ PAGE and stained with Coomassie Brilliant Blue (B) The purified 6His-tagged wild-type Vps4p and Vps4p mutant proteins were assayed in vitro for ATPase activity at 30 °C ATPase activity is...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... screening Error-prone PCR and DNA shuffling were separately used to create a randomized library of protein variants based on the Q143A hMnSOD template One hundred and fifty thousand QC774 transformants ... cloning site of the vector by digestion with NcoI and PstI This vector backbone was excised from an agarose gel and purified For both standard and error-prone PCR amplification of hMnSOD genes, ... grant GM54903 (DS) and National Science Foundation CAREER Award Bes-0348107 (HZ) We thank Patrick Quint and Diane Cabelli for help with kinetics, and Chingkuang Tu for assistance and helpful discussion...
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Báo cáo khoa học: Alpha-fetoprotein antagonizes X-linked inhibitor of apoptosis protein anticaspase activity and disrupts XIAP–caspase interaction ppt
... incubated with rhXIAP and AFP or HSA, and protein complexes were thereafter immobilized on Ni–Sepharose beads After extensive washing the supernatant and pellets (beads) were blotted and probed with ... blue and red, respectively The BIR2 and BIR3 domains are shown in green The dashed yellow lines connect the ATIF peptides of each AFP monomer and the IBM-interacting grooves of the BIR2 and BIR3 ... necessary and sufficient for cytochrome c to activate caspases and trigger cell death [9,16] The activity of IAPs is regulated by a group of IAP-regulatory proteins that bind to IAPs and inhibit...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... denaturing SDS buffer and loaded onto a single lane Activity levels are indicated as follows: (+), measurable NAAG-hydrolyzing activity; (+/–), extremely low activity; (–), no activity; ND, not determined ... buffer and 10 lL of the mixture was loaded onto a single lane Activity levels are indicated as follows: (+), measurable NAAG-hydrolyzing activity; (+/–), extremely low activity; (–), no activity; ... electroblotted onto a nitrocellulose membrane, and immunostained as described in the Materials and methods Relative band intensities were recorded using a CCD camera, and the concentrations of individual...
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