morphology of undifferentiated human embryonic and induced stem cells grown in suspension and in dynamic cultures
Characterization of adult human bone marrow mesenchymal stem cells for effective myocardial repair
... bodies composed of -actinin, the N-terminus of titin, nebulin, and telethonin contribute to the polarized organization of the thin actin filaments to form I-Z-I bands in developing sarcomeres11, ... Likewise, M-band proteins such as myomesin, M protein and the C-terminus of titin play a significant role in incorporating the myosin thick filaments into regular A bands19-25 Importantly, the coincident ... differentiation of stem cells into well-defined lineages in vitro, by introducing ECM molecules into the culture milieu In addition, cell-ECM interactions may promote the proliferation of these adult stem cells...
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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx
... Osteogenic differentiation of hADSCs Compared to non -induced control (A), Alkaline phosphatase staining was increased after being induced for weeks (B) (Bars = 100 μm) and ALP activity was quantified ... Alizarin red staining (D: non -induced control; E: induced for weeks) (Bars = 100 μm) (F) The expression of the osteogenic specific marker osteopontin (OPN) was detected by sqRT-PCR, lane 1: non -induced ... Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and...
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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1
... Carmeliet and Jain (Carmeliet and Jain 2000) (B) Sprouting of the endothelial cells is induced, resulting in tip cells migrating towards the hypoxic stimulus, with stalk cells following behind New ... importance of continuous hypoxic exposure for the culture of human fetal mesenchymal stem cells in bone tissue engineering applications 3rd Tissue Engineering and Regenerative Medicine International ... staining showed greater regions of ectopic mineralisation (black stains inducated by white arrows; scaffolds were indicated by ‘S’) and a larger area of rabbit anti -human osteopontin staining in...
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Serological Diagnosis of Certain Human, Animal and Plant Diseases Edited by Moslih Al-Moslih potx
... vary according to the type of infection and viral etiology We have to take into consideration a stage of infection, incubation period, beginning of clinical appearance and dynamic of immune response ... among siblings and children under the age of In developing countries infection occurs in the first years of life and increases successively involving almost 90% of the 50year-olds In developed ... (HEp-2), human lung carcinoma cells (A549), and others (Leland, 2007) Degenerative changes in monolayer cells provide evidence of viral presence Viruses are quantified in suspension of infected cells, ...
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báo cáo hóa học:" Implantation of neural stem cells embedded in hyaluronic acid and collagen composite conduit promotes regeneration in a rabbit facial nerve injury model" doc
... staining of BrdU and S-100 Representative images of immunohistochemical staining of BrdU and S-100 A: Localization of darkly brownish stained BrdU+ cells to transected tissues of rabbits having ... staining of β-tubulin, glial fibrillary acidic protein (GFAP) and galactocerebroside (GalC) in neurosphere-derived cells cultured on poly-L-ornithine- and laminin-coated coverslips displaying ... for three days in 4% paraformaldehyde and embedded in paraffin Sections were de-waxed and stained with haematoxylin and eosin for histological examination Toluidine blue staining was performed...
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báo cáo hóa học:" Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures" potx
... myosin expression in muscle control and in the induced hFTs cells 1C) Myosin band observed in the muscle control and in the induced hFTs cells by Ponceau S membrane dyeing 1D) IF assay, indicating ... possibility of using stem cells for regenerative medicine has opened a new field of investigation to find the best sources for obtaining multipotent stem cells, in particular through non-invasive ... FBS, 100 IU/mL penicillin (Invitrogen) and 100 IU/mL streptomycin (Invitrogen, Carlsbad, CA) in plastic flasks (25 cm2), and maintained in a humidified atmosphere of 5% CO2 in air at 37°C The culture...
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Báo cáo hóa học: "Hybrid approach of ventricular assist device and autologous bone marrow stem cells implantation in end-stage ischemic heart failure enhances myocardial reperfusion" doc
... difficult to determine, imaging of transplanted stem cells is crucial in order to investigate the attitude of the engrafted stem cells to the hosting myocardium [26] The number of treated patients ... al Journal of Translational Medicine 2011, 9:12 http://www.translational-medicine.com/content/9/1/12 Figure Intraoperative view showing clinical application of stem cells into the failing heart ... support (Figure 1) A stem cells injectate including a mixed population of endothelial progenitor cells (CD133 + ), haematopoietic stem cells (CD34 + ), and Figure A full range of cardiac support...
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SEROLOGICAL DIAGNOSIS OF CERTAIN HUMAN, ANIMAL AND PLANT DISEASES potx
... vary according to the type of infection and viral etiology We have to take into consideration a stage of infection, incubation period, beginning of clinical appearance and dynamic of immune response ... among siblings and children under the age of In developing countries infection occurs in the first years of life and increases successively involving almost 90% of the 50year-olds In developed ... (HEp-2), human lung carcinoma cells (A549), and others (Leland, 2007) Degenerative changes in monolayer cells provide evidence of viral presence Viruses are quantified in suspension of infected cells, ...
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báo cáo khoa học: "A male case of an undifferentiated carcinoma with osteoclast-like giant cells originating in an indeterminate mucin-producing cystic neoplasm of the pancreas. A case report and review of the literature" docx
... atypia In Figure 2-B we present views of the cut surface of the cystic tumor delineating the pathological mapping of carcinoma in situ, stromal invasion and gastric invasion In the stroma of the ... spread and a much better prognosis than ordinary carcinoma [14,15], the prognosis of UC with OGCs of the pancreas originating in a MCN and indeterminate mucin-producing cystic neoplasm remains unclear ... male case of an undifferentiated carcinoma with osteoclast-like giant cells originating in an indeterminate mucin-producing cystic neoplasm of the pancreas A case report and review of the literature...
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Báo cáo y học: "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions" pdf
... http://www.jbiomedsci.com/content/18/1/37 Page of Figure Immunostaining of anti-a-actinin and anti-a MHC in cells at different time points of culturing A strong expression of both a-actinin and MHC proteins (A, B, D, E, G, ... immunostaining of aactinin of cells from three groups, while, J-L shows the fluorescent immunostaining of MHC of cells from these groups after and 10 days’ culturing Cells from CMCM and CMCM+S1P ... expression of both a-actinin and MHC proteins, but not those cells from DMEM+S1P group Figure shows the time dependent expression and the percentage of cells expressing a-actinin and sarcomeric...
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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps
... self-antigen-reactive T cells in vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the ... that administration of UC-MSCs attenuated systemic inflammation in CIA in mice UC-MSCs downregulated the production of the proinflammatory cytokines TNF-a, and IL-6 in vitro and in vivo In addition, ... modulators involved in UC-MSCs mediated T-cell inhibition, while IDO, TGF-b1 and IL-10 were mainly involved in the suppressive effect of UCMSCs on FLSs Systemic administration of human UC-MSCs in established...
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Báo cáo sinh học: " Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats" potx
... tail vein Double Immunofluorescence Staining In order to identify survival and differentiate of the transplanted MSCs, a method of double immunofluorescent staining was used Rats in the GFP and ... After blocking for nonspecific binding with buffer containing 1% bovine serum albumin, the cells were incubated for 20 minutes at 4°C with the following antibodies: anti-CD29, Phycoerythrin (PE), ... 18) Page of 10 Cells were differentiated in vitro using adipogenic and oesteogenic induction media Following weeks of adipogenic induction, the cells stained Oil red ‘O’ positive showing lipid...
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o cáo hóa học:" Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats" docx
... tail vein Double Immunofluorescence Staining In order to identify survival and differentiate of the transplanted MSCs, a method of double immunofluorescent staining was used Rats in the GFP and ... After blocking for nonspecific binding with buffer containing 1% bovine serum albumin, the cells were incubated for 20 minutes at 4°C with the following antibodies: anti-CD29, Phycoerythrin (PE), ... 18) Page of 10 Cells were differentiated in vitro using adipogenic and oesteogenic induction media Following weeks of adipogenic induction, the cells stained Oil red ‘O’ positive showing lipid...
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Báo cáo lâm nghiệp: "Effect of soil temperature upon the root growth and mycorrhizal formation of white spruce (Picea glauca (Moench) Voss) seedlings grown in controlled environments" doc
... physiologies interactions between inoculation and tem- maintained out -of- doors before the first trials; were stored at 2°C in darkness for the period Dec 87-May 88, a common B.C practice; and had ... formed only by T terrestris and E-strain Accordingly, analyses of Buds were dormant until wk prior to final harvest when most flushed, with the flushing rate independent of root zone temperature ... growth flush in July 88 The growth chambers were programmed for 14 h daily photoperiods with a constant air temperature of 20°C and soil temperatures of 3, 10 and 17°C Seedling caliper and shoot...
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Báo cáo y học: " In-vivo kinetics of inhaled 5-Aminolevulinic acid-Induced Protoporphyrin IX fluorescence in bronchial tissue" pdf
... 500 time [min] Values of PPIX-fluorescence in normal findings, findings ≤ mild dysplasia and findings ≥ moderate dysplasia plotted against the Figure time between 5-ALA application and spectroscopy ... heterogeneity of 5-ALA -induced fluorescence intensity in premalignant and malignant changes may be a distinctive feature of 5-ALA metabolism as well as the patterns of tumor invasion This was also found in ... spectroscopy Values of PPIX-fluorescence in normal findings, findings ≤ mild dysplasia and findings ≥ moderate dysplasia plotted against the time between 5-ALA application and spectroscopy Fitted...
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Báo cáo y học: " Intragenic transcriptional cis-activation of the human immunodeficiency virus 1 does not result in allele-specific inhibition of the endogenous gene" pdf
... reaction, ruling out perturbation of the system by such a strong RNA binding protein Expression of Rev instead increased the proportion of unspliced RNA, consistent with its role in RREcontaining RNA ... previously [34,47] The following day cells were incubated in hypoxanthine, aminopterin and thymidine (HAT) medium and then cloned by colony picking and expansion in complete DMEM medium Colonies ... the RNA since positive cells showed invariably two alleles of equal intensity, where in the case of technical problems there would be a higher proportion of single-allele expressing cells The...
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Báo cáo y học: "Diagnostic implications of soluble triggering receptor expressed on myeloid cells-1 in patients with acute respiratory distress syndrome and abdominal diseases: a preliminary observational study" potx
... diagnosing lung infections in the context of acute respiratory distress syndrome • Peritoneal sTREM-1 is capable of identifying an abdominal infection, including those developed in the setting of ... discordance in the findings could be due to differences in the techniques for alveolar sample acquisition, in the method of measurement of sTREM-1, or in the type of patients included in the study Anand ... diagnose lung and abdominal infections, respectively Nine patients had lung infection (without abdominal infection), had abdominal infection (without lung infection), and had both infections The patients...
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Tài liệu Báo cáo khoa học: Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation in proteins with a role in cell–cell interaction pptx
... Glutamine incorporation in Caco-2 proteins determining the relative uptake of glutamine; (b) by searching for changes in the intestinal proteome; and (c) by examining glutamine incorporation into ... analysis of glutamine-containing peptides and the corresponding isotopomer peaks at high resolution revealed significant labelling of 12 proteins, which are indicate in bold NQ, No glutamine-containing ... 71-kDa protein) The third group of proteins is involved in the regulation of the redox status in cells and the fourth group in glutamine metabolism Annexin A2 and A4 belong to a family of soluble...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010,...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... greater in hES cells than in EB cells and it was not even detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in ... names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25