... amplification by "real time" technology (7300 Real Time PCR system, Applied Biosystems, Rotkreuz, Switzerland) according to manufacturer's recommendation in the presence of primers and probes specific ... 0.001 and r = 0.629, p = 0.012 respectively) induced by HCMV pp65495–503, EBV BMLF-1259– 267, EBV LMP-2426–434 and IM58–66 HLA-A0201 restricted peptides and the numbers of CD8+ T cells stained by ... extracted, reverse transcribed and amplified by qRT-PCR in the presence of primers and probes specific for the indicated genes and β-actin house keeping gene (panels A-F) Cytokine and chemokine gene expression...
... understanding can reduce validation burden by providing better options for justifying and qualifying systems intended to monitor and control physical, and/ or chemical attributes of materials and ... QR400 probe and pretreated with SNV followed by 2nd derivative In this plot, X and Y axes represent the wavelength (nm) and absorbance, respectively 74 Figure 18: SNV followed by 1st derivative ... granules’ compactibility This dwell time dependency of plastic materials can be minimized by the decreasing dwell time which can be achieved by increasing roll speed and decreasing screw speed For...
... F and Fcor are uorescence intensity before and after the correction, and P and DA denote the initial sample absorbance at the excitation wavelength and the change in absorbance introduced by ... 1ị where SD and SRF are the respective areas under the emission spectra of the donor and a reference compound, and ARF and AD are the respective absorbances of the reference compound and donor ... apo-CRP structure induced by binding of cAMP and DNA by measuring uorescence resonance energy transfer (FRET) and uorescence anisotropy decay Two tryptophan residues, Trp13 and Trp85, were used as...
... the Fig Relative MIMS signals of the m ⁄ z ¼ 32 and 44 components versus time These mass components correspond, respectively, to O2 and to CO2 Time is given in hours since the fermentor started ... interpolated so that the points are separated by equal time intervals (see Experimental procedures for details) The points in the time series can thus be labeled by an index i The analysis starts with ... computation of the mutual information I(k) between points in the time series separated by k time intervals, i.e between points i and i + k for all values of i The mutual information I(k) measures...
... the database for land suitability classification by using integration ALES and GIS Materials and methods The FAO method is based on matching land quality (supply side, the land) versus land use ... requirements (demand side, product) The key of this method is to divide the landscape of the studied area into specific types of land units called land systems The land system concept, as explained by most ... the land unit No 25 is sand dunes and sand at river banks or coastal zone Mechanic composition of the top soil is disjointed sand Terrain form is depression, there is no active irrigation and...
... first individual) and reproductive lifespan (the length of time from the start of breeding by an individual until its death) Data are for female (lines and 2) and male (lines and 4) sockeye salmon ... higher survival) and the benefits of breeding late (more energy can be acquired by adults) The optimal breeding time for an individual is then determined by its condition andby proximity to the ... and anonymous reviewers Additional advice on relevant literature was provided by J McKay, L Rieseberg, L Rowe, R Shaw, and B Sinervo A Hendry and T Day are supported by the Natural Sciences and...
... V H B CP B C H X X R e B b R C b B C $GDG(Di($W(ƯfciăgD$lWDăpiq`Ô9fDfDf9$Ôjă9Ô DISCRETIZING TIME SERIES w b B V X B B e P B CP X R CT e b BT S S R V X R CT X F b R C 3tDă`ÔDAD(D(lAăhw9$GDfÔDc(dƯ$A9$G`9GÔ9$Ôb ... Â Â Ă z & ) h(âÔụĂể#Đc è 1âd Ă r$ & " 1l COMPUTING SCORES BY COMPARING TREES w z $YÔG`j(DhGfÔ$9`d9$fpGDe P C T X e RT b V @ X S R V HT B V RTF X V ... 2048 4096 8192 16384 32768 65536 0.01 0.015 0.02 average of |z(w)| 0.025 0.03 0.035 0.04 0.045 random.dat 0.05 ắ (Ôă(Âđrc$â c{ é y â è è è ắ đ ẻ ôé ê Đ ắ â đ đ ưô ả ă ă...
... study was designed by MJH and EANA; experimental work was done by EANA, MJH and AN; interpretation and laboratory work was conducted by MJH, EANA, AN, SK and RB; EANA, RLB, SK and MJH were responsible ... bacteriophage λ DNA and a primer pair directed against λ DNA The performance of the PCR was monitored by quantitative real -time PCR (qPCR) The mean cycle threshold (Ct) and the standard deviation ... for the HSV realtime quantitative assay, which used SYBR Green I, modified by the addition of extra cycles and a final extension at 72°C for PCR DNA product and purity were checked by electrophoresis...
... study was designed by MJH and EANA; experimental work was done by EANA, MJH and AN; interpretation and laboratory work was conducted by MJH, EANA, AN, SK and RB; EANA, RLB, SK and MJH were responsible ... bacteriophage λ DNA and a primer pair directed against λ DNA The performance of the PCR was monitored by quantitative real -time PCR (qPCR) The mean cycle threshold (Ct) and the standard deviation ... for the HSV realtime quantitative assay, which used SYBR Green I, modified by the addition of extra cycles and a final extension at 72°C for PCR DNA product and purity were checked by electrophoresis...
... [58], and synthesis of proteins and proteoglycans by cartilage cells is inhibited by static compression but not by intermittent loading [59] Other, more basic, differences between animal species and ... imaging; n/a not applicable; N/A not available with standard and polarised light and images captured and digitised using a closed-circuit television and Image Grabber software (Neotech Ltd, Hampshire, ... regions was given ethical approval by Shropshire Research and Ethics Committee and all patients gave fully informed consent Twenty-three full-depth cores of cartilage and subchondral bone were obtained...
... cartilage by Tepic and colleagues [13] Further studies have recently been carried out by Zheng and colleagues [14] and Wang and colleagues [15,16], who developed a new ultrasound system for monitoring ... the patella was used Ultrasound monitoring system The ultrasound monitoring system used in this study was originally developed by Zheng and colleagues [14-16] and modified to a 10 MHz ultrasound ... represent the standard deviation of each group *P < 0.05 by the nonparametric Kruskal-Wallis test ARR was observed between the control group and group II (P = 0.008) and between group I and II (P =...
... contributions WTS and JW-C prepared the manuscript and developed and performed the PCR assays EA and MC identified and obtained oral cancer tissue EMK and SC-U and identified and obtained cervical ... skin and anogenital mucosa were assessed (Table 2) Both biopsies and fluids have been tested A subset of specimens (anogenital/skin and oral cancers) have been assessed by both standard assays and ... entire genome of HPV6 and HPV11, respectively, were obtained from the American Type Culture Collection (ATCC) and were used to derive standard curves for HPV6 and 11 in the real time PCR assay Seaman...
... 23 Chapter 2: TCM Database Development 24 2.1 24 Introduction 2.2 Database Development Method 25 2.3 Database Structure and Access 26 2.3.1 Databaseand Source of Data 26 2.3.2 Database Access ... which include drugs' Properties and Flavors, Meridians of herbs and Toxicity property, etc Based on the theories of Yin and Yang, Viscera, Channels and Collaterals, and treatment principles of traditional ... syndrome and other syndromes Herbs with salty flavor have the effects of relieving constipation by purgation, and softening and resolving hard mass They are used in the treatment of dry stool and...
... pituitary gland secretes thyrotropin (TSH) which stimulates iodine trapping and increasing cAMP, thus thyroid hormone synthesis, and release of T3 and T4 by the thyroid gland TSH is regulated by feedback ... antibodies (TRAB) by measuring the TBII, TSAB and TSBAB in these immunized animals To obtain relative quantities, by real -time PCR, of Th1 and Th2 cytokines present in the thyroidal, splenic and orbital ... Michelle Tan and Ms Lai Oi Fah for imparting invaluable knowledge on Real -Time PCR technique and for giving me advice on how to prepare my samples and write my thesis Thanks to Dr Michelle Tan and Dr...
... and the corresponding LDT values were found in both IDCM subgroups Results Systolic and diastolic time intervals The clinical characteristics andtime interval details of the patient groups and ... processed and analyzed by a semi-automated computer routine (8) Right- and left-ventricular regions of interest were traced simultaneously on diastolic images and functional images obtained by Fourier ... block biventricular pacing typically improves the time course of cardiac contraction at rest and during exercise by reducing LV dyssynchrony and thereby liberating a further segment of the RR interval...
... explanations and arguments when marking assignments More importantly, by using smart- thinking skills to understand context—the situations in which we learn and communicate knowledge—you can understand ... political and social purposes, its biases and consequences, its exclusions and errors Thinking smart is about recognising the contexts of power and influence in which knowledge exists Thinking smart ... however, often obscured by their mode of expression By understanding what claims are and what their properties are, we can better understand how to use claims as premises and conclusions in our...
... amplitude, and that rays, or arrivals within a single cluster, also decayed with time Their model proposes that both of these decaying patterns are exponential with time, and are controlled by two time ... power within clusters The time of arrival is described by two Poisson processes which model the arrival times of clusters and the arrival times of rays within clusters The time of arrival of each ... arrival rate, and λ is the ray arrival rate 2.1.1 Model Parameters In [1], the parameters Γ and γ were estimated by superimposing clusters with normalized amplitudes andtime delays and selecting...
... 2000 Real -time PCR The microcystin-degrading bacteria were quantified by real -time PCR using QMF and QMR oligonucleotide primers, and a QMT TaqMan probe BACT1369F and PROK1492R primers and a TM1389BACT2 ... sensitively and specifically by real -time PCR using newly designed primers and a TaqMan probe The microcystin-degrading bacteria were detected using a new probe and primer sets, and quantified ... quantified by real -time PCR using BACT1369F and PROK1492R primers and a TM1389BACT2 probe Microcystin-degrading bacteria comprised approximately 0.005% of the total bacteria present in the biofilm, and...