molecular characterization of pestiviruses
Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding specificity and target candidates doc
... APUM-2 (A) Scheme of the three-hybrid strategy used in the screen (B) Number of colonies identified in each step of the screen (C) Distribution of the 63 distinct sequences in relation of their position ... Binding of APUM to NRE To test the predictions regarding the RNA-binding specificities of the putative APUM proteins, we investigated the capacity of APUM to bind to the NRE sequence of hunchback ... transcripts are potential targets for regulation by the PUF family of proteins The results obtained reveal a molecular conservation of PUF proteins in Arabidopsis thaliana and suggest that translational...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... positions of the Ca of Ala156 in HYDJs and the Ca of Met156 in HYDBp located in the SGL3, which leads to the increased size of the substrate binding pocket of HYDJs The side chain of Ala156 is ... understanding of the roles of each of these residues will enable manipulation of these SGLs by rational design or molecular evolution methods to obtain the desired catalytic activity Overexpression of heterologous ... characterization and Jannaschia sp CCS1 evaluation of HYDJs from Characterization of HYDJs To explore the possible cause of the higher specific activity for conversion of d,l-p-HPH to N-carbamoylp-hydroxyphenylglycine...
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Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf
... role was suggested for this specific isoform [10] However the physiological role of these LOXs is largely unclear Because of their role in the generation of pleasant or unpleasant flavours and ... intake of lmol of O2 per minute pH optimum of hazelnut LOX was determined as previously described [9] LOX activity was also assayed spectrophotometrically monitoring the increase in A234 of the ... in the middle of July and are harvested at the end of August The maturation stages considered in this work were identified on the basis of seed weight Stage I (harvested at the end of May 2002)...
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Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx
... (1988) Characterization of two allelic forms of Neurospora crassa laccase J Biol Chem 263, 885–896 328 S Chen et al (Eur J Biochem 271) 38 Williamson, P.R (1994) Biochemical and molecular characterization ... molecular characterization of the diphenol oxidase of Cryptococcus neoformans: identification as a laccase J Bacteriol 176, 656–664 39 Zhao, J & Kwan, H.S (1999) Characterization, molecular cloning, and ... rate of 0.5 mLÆmin)1 Pooled active fractions ( 30 mL) were concentrated with a Centriprep YM-10 centrifugal filter (Millipore) and stored at )20 °C Enzyme characterization The molecular mass of...
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Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot
... overexpression of ORP2 led to a significant reduction of ACAT activity and cholesterol esters [8] Overexpression of a splice variant of ORP4 (ORP4-S) caused a 40% reduction in esterification of low density ... processed to its mature form (M form with molecular mass of 61 kDa) Deficiency in this process leads to accumulation of and secretion of p2 CPY As shown in Fig 7, at of chase, p1 CPY was the predominant ... in the absence of Osh6p or the presence of a high level of Osh6p The discrepancy may be due to the fact that a more drastic disruption of sterol homeostasis is incurred upon loss of all Osh function...
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Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus potx
... presence of mM dithiothreitol Half-life values were calculated from a fit of the data (exponential decay: y ¼ aÆe–bx) Results Characterization based on amino acid sequence The sequences of the three ... NoxA-1 gave a clear band of the expected size (48 kDa) Expression of noxB-1 was less clear, but still visible on the gel The expected molecular mass based on the sequence of NoxB-1 is 68 kDa NoxC ... instead of FAD, or NADPH instead of NADH no NoxC activity was found For this reason, further purification and characterization of NoxC was abandoned Purification of the recombinant enzymes NoxA-1...
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Báo cáo khoa học: Molecular characterization of a novel nuclear transglutaminase that is expressed during starfish embryogenesis ppt
... that of the catalytic site-containing region of the other TGs was amplified To obtain further sequences upstream of primer TG5 and downstream of primer TG3V, we used the rapid amplification of cDNA ... of a c-carbonyl of histone H2B by esterification, followed by a nucleophilic attack by an e-amino group of Lys residue of histone H4 [38], the fact that the cross-link is formed between Gln9 of ... concentration of 200 ngÆlL)1 Twenty picoliters of the solution, along with a small amount of KF96 silicone oil (Shin-Etsu Chemical, Tokyo, Japan), were then microinjected into the germinal vesicle of an...
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Báo cáo khoa học: Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis docx
... survival of ticks, for which the blood-meal from the host is the only source of energy Elucidation of the function of HlSCP1 in the hard tick may contribute a better understanding of the physiology of ... having a molecular mass of 53 kDa and a pI of 7.5 (Fig 2A), corresponding to the predicted size of the putative protein calculated from the HlSCP1 amino acid sequence To detect the localization of ... predicted molecular mass of 53 294 Da (Fig 1) This deduced protein has a possible cleavable signal peptide of 27 amino acid residues and the preprotein has a predicted molecular mass of 50 365...
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Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx
... IMAGEQUANT software (Amersham Biosciences) Standards consisted of lL aliquots of serial dilutions of the reaction mixtures, with the moles of phosphate defined using the ATP concentration Division of the ... long and short isoforms of the enzyme Many of the tissues included in this analysis also showed a prevalence of one isoform over the other For instance in the spleen, the short isoform is the predominant ... a percentage of the stoichiometry of PKC-dependent phosphorylation of wild-type mAK-L (F) The effect of thrombin cleavage on the phosphorylation of mAK-L by PKC SDS/PAGE analysis of Coomassie-stained...
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Báo cáo khoa học: Molecular characterization of secretory proteins Rv3619c and Rv3620c fromMycobacterium tuberculosisH37Rv docx
... here in the form of SI An SI > was considered to be significant: SI = (D540 nm of activated cells – D540 nm of inactivated cells) ⁄ D540 nm of inactivated cells Characterization of Rv3619c and Rv3620c ... square fit The heat of dilution was subtracted from the raw data of titration of Rv3619c with Rv3620c (B) Far-UV CD spectra of Rv3619c, Rv3620c, and the : complex CD spectra of lM Rv3619c (j), ... 343 Characterization of Rv3619c and Rv3620c A Mahmood et al Table A comparative analysis of thermodynamic parameters of ESAT–CFP-10, Rv0287–Rv0288, and Rv3619c–Rv3620c The stoichiometry of interaction...
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Báo cáo khoa học: Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference pptx
... 5¢-region of Pem-GIH The negative PCR is indicated by )ve The actin transcript of P monodon was used as an internal control of the amount of RNA template Each panel shows tissue distribution of Pem-GIH ... of Pem-GIH cDNA in this study GIH-specific dsRNA was synthesized from a 240 bp coding region of the mature Pem-GIH The use of long dsRNA provides the possibility of generating more varieties of ... (2004) Noise amidst the silence: off-target effects of siRNAs? Trends Genet 20, 521–524 27 Quin S, Ademal M & Lane T (2005) A computational study of off-target effects of RNA interference Nucleic...
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Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt
... harvested after 24 h of culture on 2% (w/v) glucose, by using the method of Raeder & Broda [33] Amplification of a DNA fragment encoding a portion of the catalytic domain of T emersonii cel7 was ... structure solution of the native enzyme Family contains both CBHs and endoglucanases The structure of CBHs is distinguished from that of endoglucanases by the presence of loops of polypeptide chain ... 55, 303–315 46 Littlejohn, T.G & Hynes, M.J (1992) Analysis of the site of action of the amdR product for regulation of the amdS gene of Aspergillus nidulans Mol Gen Genet 235, 81–88 47 Johnson,...
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Báo cáo khoa học: Molecular characterization of artemin and ferritin from Artemia franciscana pot
... amino-acid sequence of the artemin monomer consists of 230 residues with a calculated molecular mass of 25 976 Da The ferritin cDNA (accession number AY062897) of 725 bp consists of an ORF of 516 bp, ... regulation of artemin and Artemia ferritin mRNAs Probing of Northern blots with a labeled artemin probe revealed bands of 2.1 and 3.7 kb, the former of expected length based on the size of cloned ... encoding residues 169– 230 of the ORF and flanked by 112 bp of the 3¢-UTR The ferritin probe took in nucleotides 81–380 of the cloned cDNA, corresponding to residues 3–102 of the ferritin ORF The...
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Báo cáo khoa học: Biochemical and molecular characterization of purified chicken pancreatic phospholipase A2 docx
... flow rate of mLÆmin)1 Molecular mass markers were used to estimate the molecular masses of eluted proteins Right panel: SDS ⁄ PAGE (15%) Lane 1: molecular mass markers Lane 2: 10 lg of fractions ... presence of increasing concentrations of Ca2+ (Fig 2A) No PLA2 Table Flow sheet of ChPLA2 purification; 30 g of defatted pancreas obtained from 100 g of fresh tissue was homogenized in 300 mL of buffrer ... 2009 FEBS 4547 A Karray et al A effect of bile salts on ChPLA2 activity, the rate of hydrolysis of PC by ChPLA2 in the presence of various concentrations of bile salts, at pH 9.5 and at 37 °C,...
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Báo cáo Y học: Molecular characterization of MRG19 of Saccharomyces cerevisiae Implication in the regulation of galactose and nonfermentable carbon source utilization pdf
... expected, a protein of molecular weight 49 kDa was induced from transformant bearing the p19C-S in the presence, but not in the absence, of IPTG For immunization, a protein of molecular mass 49 ... 5% of the total soluble proteins when the cell grows on galactose as sole carbon source [28,29] Phosphorylation of S699 of Gal4p has been shown to be important for activating transcription of ... energy status of the cell is an important determinant of GAL gene induction [15,25–27] This suggests that the availability of metabolic energy is a rate-limiting step in the synthesis of GAL enzymes...
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Báo cáo sinh học: " Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... retrieval of viral pathogens from infected plant tissues allows not only identification of the viral pathogens but also detailed molecular study of their genomes, generating increased understanding of ... bring to sampling and molecular study of viral crop diseases, we tested the efficacy of FTA for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene ... used to study the efficacy of FTA technology for sampling, retrieval and molecular analysis of these viruses Geminiviruses are composed of monopartite or bipartite genomes of ssDNA, 2.7–2.8 kb in...
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Báo cáo hóa học: " Molecular characterization of highly pathogenic H5N1 avian influenza viruses isolated in Sweden in 2006" potx
... North of Europe in 2006 The close genetic relationship of Swedish isolates sub-clade 2.2.2 to the contemporary German and Danish isolates supports the proposition of the introduction and spread of ... Europe experienced a peak of outbreaks of H5N1 HPAI in domestic poultry and wild birds in March 2006 – that was supposedly the consequence of an unusual westward movement of waterfowl from the Black ... Europe Evolutionary relationships of HA genes of Swedish HP H5N1 AIVs compared to genetically closely related H5N1 viruses isoEvolutionary relationships of HA genes of Swedish HP H5N1 AIVs compared...
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Báo cáo hóa học: " Molecular characterization of Umbre virus (Bunyaviridae)" pdf
... comparison of partial M RNA segments of the Umbre virus with other Orthobuyaviruses Phylogenetic comparison of partial M RNA segments of the Umbre virus with other Orthobuyaviruses Using Mega 3.0 software ... neutralization test (Calisher et al., 1984) Availability of more sequences of Turlock group may answer about placement of this group of viruses Bunyaviruses being three segmented RNA viruses ... 50°C-5 sec and extension of at 60°C for 30 cycles The partial M gene sequence was curetted with the help of KODON Software and aligned with known Gene Bank sequences of Bunyamwera serogroup,...
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báo cáo hóa học:" Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... retrieval of viral pathogens from infected plant tissues allows not only identification of the viral pathogens but also detailed molecular study of their genomes, generating increased understanding of ... bring to sampling and molecular study of viral crop diseases, we tested the efficacy of FTA for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene ... used to study the efficacy of FTA technology for sampling, retrieval and molecular analysis of these viruses Geminiviruses are composed of monopartite or bipartite genomes of ssDNA, 2.7–2.8 kb in...
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Báo cáo khoa học: "Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea" pps
... deletion of thirteen nucleotides in 3' NTR region These nucleotide deletions Molecular characterization of Japanese encephalitis virus in Korea 203 Fig Nucleotide sequence alignment of the variable ... genotype pattern of virus may be a function of time and the immune status of host populations rather than genetic potential of the virus In addition, the variable region in 3' NTR of JEV could be ... protection against JEV challenge of recent isolate Characterization of the KV1899 strain at molecular level would be an important step towards identifying those properties of the virus, which may aid...
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