module thuc hien chu ky va kiem tra chu ky so su dung chung chi so
Proteins in food processing
... and CRC Press LLC stabilized by covalent bonds such as intramolecular disulfide bonds Using again the example of lysozyme, the presence of four intramolecular disulfide bonds contributes to its ... to the C-terminus The primary structure also includes other covalently bonded structures, such as the location of disulfide bridges and the sites of posttranslational modifications of side chains ... are usually larger than monomeric proteins For example, the 11 S globulins of various monocotyledonous as well as dicotyledonous plants such as soybean, are composed of acidic and basic subunits...
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Báo cáo khoa học: Protein and mRNA content of TcDHH1-containing mRNPs in Trypanosoma cruzi pdf
... mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi Nucleic Acids Res 37, 3407–3417 Teixeira SM, Kirchhoff LV & Donelson JE (1999) Trypanosoma cruzi: suppression ... purified previously from polysome and polysome-free fractions [27] TcDHH1 must therefore be, at least partly, associated with mRNAs that are independent of the translation machinery Our analysis of ... in stress granules, such as heat shock proteins and 40S ribosomal subunit proteins Translation initiation factors (eukaryotic initiation factors and 4, eIF3 and eIF4), also typical of SGs, were...
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Báo cáo khoa học: Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates doc
... demonstrated that the disruption of stkP gene resulted in repression of genetic transformability and virulence of S pneumoniae, suggesting an important role for StkP in the regulation of various ... examples of such significant impact of an inactivation of single STPK on the phenotype affecting physiological functions [3,10,11] A few target substrates for bacterial STPKs have been identified so far ... assay with variable divalent cation concentrations (Fig 1D) Mn2+ cation was much more effective as a cofactor than Mg2+ Maximal activation was induced in the range of 0.5–1 mm, while concentrations...
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Báo cáo lâm nghiệp: " Growth and mineral content of young chestnut trees under controlled conditions of nutrition" ppsx
... the surface of the corresponding substratum of the trees that were not destructively harvested Methods Growth parameters, such as height, base diame(at soil surface), number of branches and sum ... the subsupplemented with the other fer(table VI) The substratum pH was strata were tilizers remained acidic and stable under O and slight acidification was observed under A In contrast, pH values ... exchangeable element-poor substratum such as the C Horizon of mesotropic brown soil (Verger et al, 1985, 1994) watered only with totally deionized water This growth capacity on a substratum poor in exchangeable...
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Báo cáo khoa học: "gelling agents on growth, mineral composition and naphthoquinone content of in vitro explants of hybrid walnut tree (Juglans regia x Juglans nigra)" pdf
... with acetone/water (80/20, v/v) at °C by sonication for 30 Supernatants were collected after centrifugation and solvents were evaporated in vacuo (Speed-vac) The phenolic compounds were separated ... Quantitative variations due to the extraction and analysis method were determined from replicates (extracts) of the same dry matter The coefficient of variation did not exceed 6% RESULTS Growth ... aqueous extracts contain several cations (Kordan, 1988) which are available to plant tissues (Kordan, 1980, 1981).Organic impurities, absorbing in the same UV wavelength as phenols are also present...
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Báo cáo y học: "The protective effect of licofelone on experimental osteoarthritis is correlated with the downregulation of gene expression and protein synthesis of several major cartilage catabolic factors: MMP-13, cathepsin K and aggrecanase" docx
... antigen (cell score), with the maximum score being 100% Each slide was subjected to a double-blind evaluation, which resulted in a variation of less than 5% For the purposes of statistical analysis, ... plateaus (0.5–1.0 g) was pooled to allow for the processing of a sufficient amount of tissue for RNA extraction Cartilage was suspended in a TRIzol buffer (Invitrogen; Life Technologies, Burlington, ... analysis was highly variable and, although sometimes higher than that in normal cartilage, the differences did not reach statistical significance (Fig 3) The results were somewhat similar with...
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báo cáo khoa học: " Effect of filtration on morphine and particle content of injections prepared from slow-release oral morphine tablets" ppt
... agent which is insoluble in water) The coating contains other insolubles such as iron oxide, but this was usually removed in preparing the extracts There are advantages and disadvantages in counting ... did not result in an ever-finer powder since the waxy nature of the powder resulted in some re-compaction The crushed tablet suspended in cold water gave an opaque suspension with a milky appearance ... (thousands) Cold Extract: 0.45 m Filtrate Control Extract: 0.45 m Filtrate Particle size Figure Particles75 μm or larger in syringe filtrates Particles μm or larger in syringe filtrates Numbers of...
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Báo cáo y học: " Elevated expression of both mRNA and protein levels of IL-17A in sputum of stable Cystic Fibrosis patients" potx
... therapy The same authors found elevated IL-17A protein levels in BAL fluid of children with CF also during a pulmonary exacerbation [20] Our data suggest a chronic activation of the IL-23/IL-17A axis ... statistical analysis of the results, Graphpad Prism (Graphad software Inc, San Diego, USA) was used For the comparison of two groups, a nonparametric Mann-Whitney U test was used Chi- square test was used ... Protein measurements in sputum samples The BD™ CBA Flex Set System and buffer master kit (BD Biosciences) was used to measure protein levels of IL-17A in the sputum supernatants Measurements were...
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Timing and information content of insider trades, before and after the sarbanes oxley act of 2002
... insider trading suggests that the average insider trade is a potential signal to investors about firm value Insofar as the disclosure does not occur after the news that insiders were trading ... filing date after SOX, versus 1.22% before SOX.16 Post SOX abnormal trading volumes are also significantly positive on average the day before the receipt of Form 4s by the SEC, a result potentially ... post-SOX purchases (1.48%) This suggests that, after SOX, disclosures of insider purchases in financially distressed firms signal good news Overall, for purchases, the multivariate results suggest...
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Báo cáo sinh học: "Genetic parameters for lactation traits of milking ewes: protein content and composition, fat, somatic cells and individual laboratory cheese yield" ppt
... age of the ewe Although such a result may be unexpected, Sevi et al [45] have also recently reported that somatic cell counts in Comisana ewes milk and the prevalence of subclinical mastitis were ... health traits could be improved more easily by better management than by selection Our results support this suggestion The high level of SCC in the entire population of the Churra breed suggests ... respectively Similar results for milk yield, protein content and LSCC were reported for the same breed [17] No results had been reported for the rest of the variables These results demonstrated the importance...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... intraperitoneal injection of streptozotocin (150 mg/kg body weight, dissolved in sodium citrate buffer) after overnight fasting [10] Mice in normal control group were treated with sodium citrate ... Zhejiang Chinese Medical University (Hangzhou, China) Plasmid vector PET28a and Escherichia coli host strain BL21 (DE3) were obtained from Zhejiang University Institute of Life Sciences (Hangzhou, China) ... glucose-dependent insulin secretion [3] Administration of GLP-1 can increase insulin secretion and reduce blood glucose [3] GLP-1 also promotes islet β-cell proliferation, suppresses glucagon...
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Starch and Protein Quality Requirements of Japanese Alkaline Noodles (Ramen).pdf
... in RVA tests is indicated in Fig Without inactivation, the varying levels of αamylase caused substantial variation in the RVA traces for each set of samples However, the use of mM AgNO3 resulted ... for falling number (FN) and then subjected to a series of RVA tests using AgNO3 solutions of various concentrations instead of distilled water The AgNO3 concentrations ranged from 0.01 to 12 mM ... of α-amylase inactivation in RVA tests if the prime focus is to measure the inherent pasting properties RESULTS AND DISCUSSION Effect of AgNO3 Concentration on α-Amylase in RVA Tests The rain-damaged...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... as substrates, whereas the structural basis for substrate recognition and activity was revealed by solving the structure of PRTFDC1 in com˚ plex with the product GMP at 1.7 A resolution Results ... remaining solute was scanned using a spectrophotometer The UV-absorption spectrum of the solute from the precipitated protein displayed a similar UV-absorption spectrum as a GMP solution, suggesting ... concentration of hypoxanthine available for salvage in human tissues is 8.2 ± 1.3 lm [28,29] compared to the Km for PRTFDC1, which is 23 lm, indicated that PRTFDC1 is not turning over this substrate...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... endogenous IL-1b transcript level (by approximately 60%) in comparison to control cells transfected with scrambled siRNA In contrast, cells overexpressing MCPIP had lower IL-1b transcript level ... densitometry measurements were performed using Quantity One software, and transcripts amounts were normalized to the rRNA level In order to analyse the tissue distribution of the MCPIP transcript, ... PCR Transient transfection For transient transfection experiments, HepG2 cells and human fibroblasts were cultured in six-well plates to approximately 80% confluency The next day, cells were transfected...
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Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc
... concentration was calculated from the absorbance of stock solutions measured at 280 nm, using the extinction coefcients of model compounds [34] Fluorescence measurements All uorescence spectra ... magnitudes leaded to a resultant value of DGÂ that decreased linearly as the urea concentration increased (Fig 6C) Conversely, at 318 K, DHÂ and DSÂ also had a positive value, but the absolute magnitude ... m- and transition midpoint-values Fluorescence spectra at different urea concentrations were processed using the wavelength averaged emission intensity, [32] Circular dichroism measurements...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
... synergize to prolong allograft survival J Exp Med 179, 341–346 Hirahara, H., Tsuchida, M., Wanatabe, T., Haga, M., Matsumoto, Y., Abo, T & Eguchi, S (1995) Long-term survival of cardiac allografts ... intramolecular hydrogen bonded, suggesting a stable b-turn of Leu7Val8-Ala9-Glu10 [23] The temperature coefficient of chemical shift of Ser5 amide resonance showed a value >)3.0 p.p.b.ÆK)1 suggesting ... Briefly, the linear peptide was subjected to MD simulations in vacuo at 300K with ROE and disulfide bond constraints [26] The resulting structure was cyclized by forming disulfide bonds The cyclic structure...
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Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
... neurones of the central nervous system, although it has also been reported to be expressed in some other tissues [18,26] To investigate whether Ras-GRF1 plays a role in the activation of Ras ⁄ ERK ... medium with supplements, as described above, prior to starvation in DMEM without serum for the last 16–20 h before serotonin treatment and lysis ( 48 h after transfection for transiently transfected ... HRP-conjugated secondary antibodies were visualized with SuperSignal Dura West extended-duration chemiluminescent substrate and analysed with a UVP BioChemie system isolate mRNA from the pool of total...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... dynamics Quenching experiments by acrylamide in the presence of several combinations of substrates and divalent ions at saturating concentrations were carried out by measuring the quenching of the ... mutated to glycine, and the solvent accessible surface area for the index residue is calculated (reference value for 100% solvent accessible surface) The reference value for each residue is dependent ... reported values for the native enzyme (Table 1) This shows that the derivatized enzyme, even when inactive [7], retains similar afnity for the substrates This suggests that the enzyme inactivation...
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Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx
... decrease in peroxide concentration, and a subsequent slow decay (Fig 1) The absolute levels of peroxide lost in the rapid phase after addition of catalase, was substrate dependent (Fig 1) The ... the requirement for high concentrations of NEM can result in other modications [39] Subsequent incubation of such NEM-treated GAPDH with N-Ac-Trp-OMe peroxides resulted in a much slower, and less ... enzyme This process gives a sulfenic acid (R-SOH), which can be repaired by dithiothreitol The isolated enzyme can also be inhibited by UV light [46], nitric oxide [37], superoxide radicals [42],...
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