... transplanted with Sall4 expanded cells Strain Number of mouse C57B/6 Sall4A expanded cells, × 106 17 WT-like B6.SJL Sall4A expanded cells, × 106 15 WT-like B6.SJL Sall4A expanded cells, × 106 11 WT-like ... Interestingly, Sall4 is one of the few genes that are also involved in adult tissue stemcells [17,18], and its protein expression is always correlated with the presence ofstemandprogenitor cell populations ... cells, and generation of induced pluripotent stemcells (iPS) [11-13] We and others have determined that Sall4 exists as two isoforms (Sall4A and Sall4B), and they have unique and overlapping functions...
... significant mobilizationofcells expressing hematopoieticstem cell markers Data is presented as percentage of mononuclear cells in Additional File Analysis of the number of the progenitorcells in ... Quantification of peripheral blood cells expressing the hematopoieticstem cell markers CD133 and CD34 was performed at day (pre-treatment) and on days 1, 2, and 14 subsequent to initiation of Stem- Kine ... Chandler AZ), was capable of increasing the number of circulating stemcellsandprogenitorcells This proprietary food supplement is produced by fermentation of a combination of green tea, astralagus,...
... hematopoieticcells to ALCAM expressing components of the hematopoietic niche via dimerization We investigated the role of ALCAM on the homing abilities ofhematopoieticstemandprogenitorcells ... Isothiocyanate HN Hematopoietic Niche HSC HematopoieticStemCells HSPC HematopoieticStemandProgenitorCells Marrow ECM Marrow Extracellular Matrix MSC Mesenchymal StemCells OB Osteoblasts ... price of this thesis, as he suffered through it with me and paid the greater portion of it iii ABSTRACT Mariya Aleksandrova Aleksandrova IMPACT OF ALCAM (CD166) ON HOMING OFHEMATOPOIETICSTEM AND...
... by Lipofectamine 2000, and then cells were exposed to either normoxia (20% O2) or hypoxia (10% O2) for days The percentage of GFP-positive cells was about 70% of that of total neural stemcells ... growth of most kinds of cells, promote the proliferation of some types ofcells There is increasing evidence that mild hypoxia acts as a potent regulator of various types ofstemcells [12] Therefore, ... neural stemcells derived from the embryonic mesencephalon (E13.5) were used in the present study Generally, neural stemcells were grown to form as neurospheres in vitro The ability ofstem cells...
... adult progenitorcells (MAPCs), are the stemcells Page of 15 forming bone marrow [33] Their role is maintenance and turnover of blood cellsand immune system The high rate of regeneration of the ... functional repair of myocardial infarction, and in stroke [31,32] Other types ofstemcellsHematopoieticstemcells together with mesenchymal stem cells, the so-called “side population”, and multipotent ... Induced pluripotent stemcells Induced pluripotent stemcells (iPSCs) from somatic cells are revolutionizing the field ofstemcells Obtained by reprogramming somatic stemcellsof a patient through...
... epitope profile of tonsil-derived mesenchymal progenitorcells (T-MPCs) (a) Immunofluorescence analysis of cell surface epitope profiles of T-MPCs and bone marrow-derived mesenchymal progenitorcells ... T-MPCs and BM-MPCs of the same passage maintained and cultured in expansion medium, and BM-MPCs cultured in adipogenic medium Morphological changes in BM-MPCs and T-MPCs and the formation of cyto- ... of proliferation of alloreactive T cells The MLR was used to test the immunosuppressive properties of T-MPCs and BM-MPCs Initially, using human PBMCs from healthy donors as responding cells and...
... between the EPO therapy and placebo-control group of 0.32%, and a standard deviation of circulating level of EPCs in EPO therapy was 0.7% A 20% rate of protocol violations and incomplete follow-up ... death The secondary objective of this study was to establish the time course of circulating levels of EPCs in patients after acute IS and the ability of two doses of EPO in enhancing circulating ... processes [15,16], neovascularization, mobilizationof endothelial progenitorcells (EPCs), and angiogenesis [21-23] An increase in circulating levels of EPCs in patients after acute IS has been...
... potential is often impaired and liver progenitor cells, also called oval cells, significantly increase both in number and their capability to proliferate [2,3] In recent years, liver progenitorcells ... hybridization and data mining Pipeline profiling of transcriptional and translational changes occurring during hepatocytic differentiation of HepaRG cells Figure for Pipeline for profiling of transcriptional ... differentiation status of the cells Cell death was also a top function of network C (Additional data file 1, C) with the presence of another member of the initiator caspase family, caspase 9, andof FOXO3A,...
... The Law of Corporate Finance: GeneralPrinciplesand EU Law Petri M¨antysaari The Law of Corporate Finance: GeneralPrinciplesand EU Law Volume II: Contracts in General 123 Professor Petri ... common coding for standards, IT system for cataloging and sharing the standards, creation of standards, definition of work processes for usage of standards); step five – use standards; step six ... Contracts in General: The Legal Framework clusion of a contract, the form and the content of an offer, and the acceptance of an offer) There are also directives that specify the content of the information...
... The Law of Corporate Finance: GeneralPrinciplesand EU Law Petri M¨antysaari The Law of Corporate Finance: GeneralPrinciplesand EU Law Volume III: Funding, Exit, Takeovers 123 Professor Petri ... commercial, and legal advantages and disadvantages of different sources of funding The funding mix depends on: the availability and cost of capital; corporate risk management and the management of agency ... include: access to funding; the mechanism of raising funds; the management of costs and the mechanism of payment of costs; and the repayment of funds The modalities of the transaction are, to a large...
... (CD123) and the CD4 [18] Moreover, as a hallmark of pDC, these cells are BDCA2 and BDCA4 (type II C lectin)-positive and CD11c- and CD1a-negative Trypan blue exclusion and cell counting of LV and ... and non-adherent cells in culture were harvested at day and transduced by LV-VSVG-PGK at an MOI of 18 TU/ml and at a fixed concentration of × 106 cells/ ml, in RPMI 1640 After hours at 37°C, cells ... concentration of 2–5 × 106 ofcells per 200–500 μl of medium After hours at 37°C, cells were placed in complete medium and analysed by flow cytometry between day and day 60 Transduction of CD34-pDC...
... potential of non hematopoieticstemcells Because of the literature focusing on the potential effect of endothelial progenitors, we did not perform Lin + cells experiment Recently Cappocia and col ... CD11b+ cells could be responsible of angiogenesis via a paracrine effect In our model, we cannot rule out a potential beneficial effect of Lin + cells The importance of endothelial progenitorcells ... obtain a beneficial effect and to improve repair in the lung However, the detection of a low engraftment ofcellsand the results obtained from BMDC purified stemcells injection in our model...
... structures and members for buildings and civil engineering works It complies with the principlesand requirements for the safety and serviceability of structures, the basis of their design and verification ... 1-1: General rules and rules for buildings Part 1-2: Structural fire design Part 2: General rules and rules for bridges 1.1.2 Scope of Part 1-1 of Eurocode (1) Part 1-1 of Eurocode gives a general ... side of the web Distance between the centres of the outstand shear connectors; mean width of a concrete rib (minimum width for re-entrant sheeting profiles); width of haunch Width of the outstand...
... was 1.8°, the standard deviation of the hydrogen bond energies was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant ... in position With the exception of four a-helices and two pairs of short b-strands, the rest of the protein consists almost entirely of loops connecting the b-strands The loops extending from the ... Denaturation and refolding of reCBH and enzyme assay reCBH (1 mg) was denatured by incubation in a solution of M urea/0.1 M Tris/HCl, pH 8.0, in the presence of 100 mM dithiothreitol and mM EDTA,...
... the number of disulphide bridges, the location of proline and arginine residues, and hydrophobic core interactions are generally recognized as key factors in determining the flexibility of the enzyme ... of charged residues on the surface which enhances flexibility and ability of interaction with the solvent [3] Both factors are of importance for cold activity On the other hand, the presence of ... coexpression of rPFL with the foldase of P aeruginosa did Fig Alignment of the PFL sequence with Pseudomonas lipases of known three-dimensional structure The sequence is identical to that of the lipase...
... known whether p-BSQ of CS causes apoptosis and emphysematous lung damage We have addressed this question for better understanding of the cellular andmolecularmechanismsof emphysema, so that ... and Bcl-2 of the lung extracts of guinea pigs exposed to air or CS supplemented with A, Immunoblots of caspase 3, PARP, A, Immunoblots of caspase 3, PARP, Bax and Bcl-2 of the lung extracts of ... of DNA strand breaks (TUNEL assay) in lung cellsof guinea pigs exposed to smoke from 1, and cigarettes C, Quantitative evaluation of TUNEL assay and protein carbonyl formation in the lungs of...
... understood Hematopoieticstemcells (HSCs) contain specific markers on their surface such as CD34 and CD133 allowing for the isolation of relatively pure populations of these cells for in vitro study of ... because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and ... Isolation and culture ofhematopoieticstemcells (HSCs) Bone marrow samples (BM) from Hb E/b-thalassemic patients and healthy donors were collected for HSCs isolation Briefly, mononuclear cells...
... understood Hematopoieticstemcells (HSCs) contain specific markers on their surface such as CD34 and CD133 allowing for the isolation of relatively pure populations of these cells for in vitro study of ... because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and ... Isolation and culture ofhematopoieticstemcells (HSCs) Bone marrow samples (BM) from Hb E/b-thalassemic patients and healthy donors were collected for HSCs isolation Briefly, mononuclear cells...
... my two supervisors Prof Teoh Swee-Hin and A/Prof Jerry Chan I would like to thank them for their constant sharing of knowledge and ideas, their patience and understanding, and the many hours they ... Human endothelial progenitorstemcells accelerates and potentiates the osteogenic response of bone marrow derived human fetal mesenchymal stemcells through paracrine signalling mechanisms International ... Human endothelial progenitorstemcells enhances osteogenic response of bone marrow derived human fetal mesenchymal stemcells through paracrine signalling mechanisms in vitro and induces neovasculogenesis...
... 1.1 Hematopoieticstemandprogenitorcells (HSPC) Stemcells hold great promise for regenerative medicine and the study of cell and tissue differentiation Hematopoieticstemandprogenitorcells ... of CD137 and CD137 ligand on small subsets of bone marrow cells Activation ofhematopoieticprogenitorcells – CD34+ cells in the human system and Lin-, CD117+ cells in the murine system – through ... TABLE OF CONTENTS ACKNOWLEDGEMENT ii TABLE OF CONTENT iv ABSTRACT x LIST OF TABLES xi LIST OF FIGURES xii LIST OF ABBREVIATIONS xvi CHAPTER INTRODUCTION 1.1 Hematopoieticstemandprogenitor cells...