metabolic map of grr1δ cells grown on 2 glucose previous page gene and protein expression measurements were overlaid onto a metabolic map of s cerevisiae metabolism enzymes are represented by nodes with the color of the node denot
... 30 24 0 at 37 °C The media andcellswere separated andthe 59Fe levels in each assessed Cellswere lysed andthe cytosols subjected to native PAGE- 59Fe-autoradiography (see Materials and methods) ... cellswere separated andthe 59Fe levels in each assessed Cellswere lysed andthe cytosols subjected to native PAGE- 59Fe-autoradiography (see Materials and methods) (B) Native PAGE- 59Fe-autoradiographs ... levels in each assessed Cellswere lysed andthe cytosols subjected to native PAGE- 59Feautoradiography (see Materials and methods) (B) Native PAGE- 59Fe-autoradiographs of cellular cytosols treated...
... In approximately 2% ofthe withdrawal cases, the cause of cessation was registered as both treatment failure and AE; for these patients, the cause of withdrawal was subsequently classified as an ... registered: health assessment questionnaire (HAQ) score, patient-scored visual analogue scale for pain (VASpain) and general health (VASglobal), physician 's global assessment of disease activity ... retrieved results for this study Statistical analysis Baseline clinical characteristics were analysed bythe KruskalWallis and Mann-Whitney U tests for comparison of groups for continuous variables, whereas...
... induction, most ofthecellsare still in the transcriptionally inactive state and no protein is synthesized; they constitute the left-peak population in the histogram As soon as thegene template ... level as described in the present study; the other is binary activation of TA in response to upstream signaling With stochastic gene activation, the all-or-none response ofproteinexpression lies ... lifetime and high detection sensitivity favor the appearance of binary proteinexpressionThe reverse conditions favor the appearance of graded proteinexpression Binary responses observed with commonly-used...
... 1.8 .2. 1 Array Fabrication 1.8 .2. 2 Probe Preparation and Hybridization 1.8 .2. 3 Data Collection and Analysis 1.8 .2. 4 Data Validation, Quality, and Statistical Issues 1.8 .2. 5 Limitations ofExpression ... asthma and atopic dermatitis), late phase reactions are pronounced The late phase manesfestion includes These responses are caused bythe activation of allergen-specific T cells after hours to days, ... the respiratory tract Inhalation of dust mite allergens by sensitive individuals can cause allergy diseases such as bronchial asthma, allergic rhinitis, atopic eczema, andare occasionally fatal...
... isocitrate lyase and malate synthase TheScerevisiaeenzymes Icl1p, Mls1p, Mdh2p, Cit1p, and Aco1p are noted, these being essential for growth of yeast cellson C2 carbon sources such as ethanol ... in cellsgrownon media supplemented with ethanol or oleic acid MATERIALS AND METHODS Strains, plasmid constructions andgene disruptions Scerevisiae strains, plasmids and oligonucleotides used ... mitochondrial citrate synthase encoded by CIT1 (and possibly also by CIT3), cytosolic Mdh2p, and extraperoxisomal Aco1p As mentioned previously, malate synthase catalyses the formation of malate...
... also the role of phosphate in reducing the uptake of Cu by Brassica juncea L 2.2 Sampling and chemical analysis Plant and soil samples were taken and analysed at the harvest time (45 days after ... plant height and biomass Total Cu in soil and plant tissues, Cu2+ (HNO3 0.43N) in soil was determined by Atomic Absorption Spectroscopy (AAS) Materials and methods Results and discussion 2. 1 Pot experiments ... Effects of Cu application on plan growth and Cu accumulation in plant The results ofthe effects of added Cu on plant growth and heavy metal accumulation in Brassica juncea L plants are presented...
... and A5 49 cells [30] In order to establish the validity ofthe EA.hy. 926 cells as a model for studies on cPLA2 -a in endothelial cells, expression levels of cPLA2 -a in EA.hy. 926 cellsand HUVECs were ... compared Equivalent amounts ofproteinwere separated by SDS ⁄ PAGEand immunoblotted for cPLA2 -a Lysates from the parental A5 49 cell line and HeLa cellswere also analysed The results (Fig 1A, B) ... and site of cPLA2 -a relocation Comparison ofthe location of cPLA2 -a in EA.hy. 926 endothelial cellswith markers for specific cellular organelles The relocation of cPLA2 -a in endothelial cells...
... immunocytochemistry stains (Panel A, 20 0×) Alkaline phosphatase (ALP) activity of osteoblasts was assayed using an ALP kit and visualized as the pink color (Panel B, 20 0×) In addition, von Kossa staining was ... out the cell culture, scaffold preparation, biochemistry assessment, acquisition, and analysis of data SYY participated in the image and statistical analysis All authors read and approved the ... ofthe ALP assay was normalized against the amount of total DNA in each sample The nitric oxide generated by endothelial cellson scaffolds was assessed using the Nitric Oxide Colorimetric Assay...
... materials, such as GaSb/GaAs [8-10], InAlAs/InP [11], InP/InGaP [ 12, 13], InP/GaAs [14], GaAsSb/GaAs [15], and InAs/GaSb [16, 17] The reason is that they offer comparatively large bandgap energies ... height size distribution of GaSb/ InGaAs QDs on InP (100) substrate, the AFM and STEM measurementswere carried out Figure shows the AFM and STEM images of GaSb/In0.53Ga0.47As QDs andthe histogram ... and STEM images of GaSb/In0.53Ga0.47As QDs and histogram ofthe height of GaSb/In0.53Ga0.47As QDs (a) The AFM image of GaSb/In0.53Ga0.47As QDs, (b) histogram ofthe height of GaSb/In0.53Ga0.47As...
... this study, 2, 4, and cycles of Ag2 S deposition were performed (denoted as Ag 2S (2) , Ag 2S( 4), and Ag 2S( 8), respectively) Finally, the as-prepared samples were dried in a N2 stream The preparation ... spherical nanoparticles onthe TNTs andthe wall thickness ofthe Ag S( 4)/TNTs is similar to that ofthe plain TNTs In addition, a uniform distribution ofthe Ag 2S nanoparticles with diameters of approximately ... increase in the cycles of Ag 2S deposition, resulting from an increased amount of Ag 2S nanoparticles For the performance comparison of as-prepared Ag2Ssensitized TNT and ZnO/TNT electrodes, the...
... Authors’ contributions RHM carried out DLTS and LDLTS measurements, prepared figures and wrote the first draft MS, MA, AK and MH participated in the analysis ofthe data andthe preparation of ... their argument Experimental details A set of six AlGaAs samples with different Be-doping concentrations grownby MBE on semi-insulating (100) and (311 )A GaAs substrates have been studied The samples, ... field-dependent studies that both charged and neutral like traps exist in the samples The traps showing the effect of electric field onthe carrier emission rates are ionised after carrier emission and carry...
... magnetic domains are randomly distributed in MFM images for MnAs films grownon both GaAs (110) and GaAs (001) We also observed the cross-sectional MFM images for MnAs/GaAs (001) and MnAs/GaAs ... films have a well-ordered crystal orientation anda sharp interface between MnAs and GaAs Judged from the chromatic aberration of MnAs and GaAs substrate, the thickness ofthe epitaxial MnAs film ... carried out the MFM and SQUID measurements, the statistical analysis and drafted the manuscript JZ conceived ofthe study and participated in its design and coordination All authors read and approved...
... character ofthegrown SLEG Figure SEM images ofa monolayer graphene islands grownonthe C-face of an 8° off-axis 4H-SiC substrate (a, b) Images ofthe largest homogeneous SLEG islands, (c) early ... http://www.nanoscalereslett.com/content/6/1/141 Pageof Figure AFM images of continuous and almost free standing monolayer graphene islands grownonthe C-face of an 8° off-axis 4H-SiC substrate (a) at a ... shown the possibility to grow large islands of monolayer graphene onthe C-face of on- axis and 8° off-axis commercial 4H-SiC wafers The graphene layers are continuous, almost free-standing and show...
... (311)B sample shows two peaks associated with defect states labelled EB1 and EB2 Trap EB1 appears as a shoulder ofthe main peak EB2 at temperature *390 K and is resolved by using LDLTS as shown ... 2. 6 2. 7 2. 8 2. 9 -1 1000/T (K ) Fig a DLTS spectra of GaAs/AlGaAs multi-quantum well structures grownon (100) and (311)B GaAs substrates The inset shows the peaks resolved by Laplace DLTS technique; ... the Al composition Their results show that the decrease in the thermal emission rates with increasing field is strongest for the layers having medium Al compositions (Al: 30–40%) and smallest for...
... height size distribution of GaSb/InGaAs QDs on InP (100) substrate, the AFM and STEM measurementswere carried out Figure shows the AFM and STEM images of GaSb/ In0.53Ga0.47 As QDs andthe histogram ... GaSb/In0.53Ga0.47As QDs (a) The AFM image of GaSb/In0.53Ga0.47As QDs, (b) histogram ofthe height of GaSb/In0.53Ga0.47As QDs, and (c) the STEM image of GaSb/In0.53Ga0.47As QDs Shuhui et al Nanoscale Research ... histogram ofthe height of GaSb/In 0.53 Ga 0.47 As QDs As shown in Figure 1a, the statistical data indicate that the density ofthe QDs is approximately × 109 cm -2 and that the shape of GaSb QDs is rectangular-shaped...
... layer is grownonthe GaAs(111)B substrate To ensure n-type doping ofthe buffer layer, a separate sample has been grownonthe GaAs(111)B semi-insulating substrate at the same growth conditions ... oxygen plasma until the tips of GaAs NWs are exposed Conventional Ohmic contacts for the backside of n-type substrate are fabricated by electron-beam evaporating of AuGe (30 nm) and Ni/Au (10/150 ... we also present the schematics of device structure (Fig 1d) Results and Discussion I–V characteristics are measured using a Keithley 23 8 source meter The samples are placed ona copper base from...
... height size distribution of GaSb/InGaAs QDs on InP (100) substrate, the AFM and STEM measurementswere carried out Figure shows the AFM and STEM images of GaSb/ In0.53Ga0.47 As QDs andthe histogram ... GaSb/In0.53Ga0.47As QDs (a) The AFM image of GaSb/In0.53Ga0.47As QDs, (b) histogram ofthe height of GaSb/In0.53Ga0.47As QDs, and (c) the STEM image of GaSb/In0.53Ga0.47As QDs Shuhui et al Nanoscale Research ... histogram ofthe height of GaSb/In 0.53 Ga 0.47 As QDs As shown in Figure 1a, the statistical data indicate that the density ofthe QDs is approximately × 109 cm -2 and that the shape of GaSb QDs is rectangular-shaped...
... shows AFM images of InAs QDs grownon (In)GaAs buffer layer grownon GaAs Fig AFM images for InAs/ (In)GaAs QDs grownon GaAs (311) B and (100) substrates a InAs/InGaAs (311) B; b InAs/ InGaAs (100); ... IF bonds consisted of In–Sb bonds rather than the Ga–As bonds [20 , 21 ] This is due to additional IF strain offered bythe higher atom sizeof In and Sb than that of Ga and As However, one cannot ... of larger size onthe GaAs (100) substrates than those on GaAs (311) B substrates The average density, height, and lateral size for these two samples are 4.8 108 cm -2 and Nanoscale Res Lett (20 09)...
... shown as a bright spot spontaneously bythe formation ofthe IMF at the GaSb/ GaAs interface Further proof of spontaneous relaxation of IMF-based samples is provided via the SAED double spot pattern ... that the GaSb layer is completely relaxed Further proof of strain is also achieved from SAED measurements, which shows that GaSb and GaAs has lattice constants almost similar to the expected lattice ... atoms are deposited on Ga-rich GaAs surface before starting the growth of bulk GaSb epitaxial layer If Sb is deposited on As-rich GaAs surface instead of Ga-rich GaAs surface, the resulting epitaxial...