measurement of oxidized and reduced coenzyme q in biological fluids cells and tissues an hplc ec method
Báo cáo khóa học: C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins docx
... denitricans electron transfer avoprotein, and 13Cand 15N-NMR spectroscopy of human electron transfer avoprotein in the oxidised and reduced states Eur J Biochem 255, 125132 36 Bourn, A.J.R & Randall, ... shifts (in p.p.m.) of native and native reconstituted thioredoxin reductase (TrxR), and of the native mutant protein E159Y in the oxidized and reduced states For comparison the chemical shifts of ... C(4), C(8) and C(9) of the enzymes are least affected by reduction, and those of C(6) and C(7) are upeld shifted by a p-electron density increase coming from N(5) and N(10), respectively In contrast...
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Báo cáo y học: " Invasive versus noninvasive measurement of allergic and cholinergic airway responsiveness in mice" doc
... the standard and new measurements is unlikely to mislead in this way Accordingly, the agreement between the invasive and noninvasive lung function methods was analyzed by the method of Bland and ... in simultaneous invasive measurements of GL, EF50 and Cdyn for allergic and control mice during EAR and cholinergic AR Values are means ± SD (95 % confidence intervals (CI) in brackets) for animals ... significantly overestimate the actual changes in lung resistance after 24 and 48 h of hyperoxia Interestingly, increases in Penh were accompanied by decreased TE and rising VT and f This contrasts with the...
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The measurement of time and income poverty in korea
... conducted jointly by scholars in the Distribution of Income and Wealth and Gender Equality and the Economy programs The central objective of the project is to develop a measure of time and income ... income-poor 10 and time-nonpoor; (c) income-nonpoor and time-poor; and (d) income-nonpoor and timenonpoor 2.2 Empirical Methodology and Data 2.2.1 Statistical Matching The measurement of time and income ... money income in the welfare survey by adding up the following sources of household income: wage and salaries; business income; capital income; social security; social insurance and assistance; and...
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Control and measurement of clamping and machining forces in intelligent fixturing
... sensitive indicators of machining performance Force measurement is an important aspect in machining performance analysis, flexible manufacturing systems, precision machining, intelligent fixturing and ... the analysis and synthesis of fixtures used in manufacturing This shows the importance of fixturing in manufacturing processes and the need for research and development in this field One of the ... manufacturing systems, require the measurement of clamping and cutting forces This research addresses pertinent issues in both cutting force measurements and control of the clamping force and an attempt...
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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
... factor increases syndecan-1 and syndecan-4 mRNAs expression in ®broblasts [48] and in vascular smooth muscle cells [49], respectively At the transcriptional level, the 5¢ ¯anking region of syndecan-1 ... bFGF itself could in uence syndecan-1, syndecan-4 and glypican-1 mRNAs expression bFGF effect on glypican-1, syndecan-1 and syndecan-4 mRNAs expression The relative mRNA expression of these HSPG ... ngáml)1 FSH and increasing concentrations of bFGF (lanes to 6) Total RNA was extracted as described in Materials and methods Then, 500 ng RNA was reverse-transcribed and ampli®ed by relative quantitative...
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Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf
... Materials and methods Anandamide hydrolase activity and expression Materials Chemicals were of the purest analytical grade Leptin (human recombinant), anandamide (N-arachidonoylethanolamine, AEA), ... dissociation constants (Kd) of 2.0 ± 0.1 and 2.2 ± 0.2 nM and maximum binding (Bmax) of 382 ± and 339 ± fmolÆmg protein)1, for U937 cells and CHP100 cells, respectively These values are in agreement ... of FAAH in human U937 and CHP100 cells and on the protein content and the mRNA of FAAH in U937 cells These cells were incubated for 24 h with leptin alone, or with 10 nM ( 150 ngÆmL)1) leptin...
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Báo cáo y học: "Preformulation and stability in biological fluids of the retrocyclin RC-101, a potential anti-HIV topical microbicide/; potx
... between and indicates an intermediate state, and between and 10 indicates bacterial vaginosis [14] Subjects were notified of the test results by telephone within two weeks of collection and directed ... contribution in performing the human samples collection BLH and TPC have made substantial contribution in developing and conducting the analysis for the LC-MS/MS method for protein detection in biological ... preformulation of a recombinant ricin toxin A-chain vaccine J Pharm Sci 2007, 96:44-60 Kikwai L, Babu RJ, Kanikkannan N, Singh M: Preformulation stability of Spantide II, a promising topical anti-inflammatory...
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Bóa cáo y học: "Erroneous measurement of haemodynamic parameters by PiCCO™ monitor in a critically ill patient with renal replacement therapy: a case report" pot
... measurement with the help of EC- A, who was in charge of the patient the following day All authors participated in the draft of the manuscript, and read and approved the final version Acknowledgement ... alteration of the area under the curve obtained by the injection of cool saline We think the same alteration would be produced by any catheter, if calibration is done during haemodialysis, with a working ... A, Reinhart K, Meier-hellmann A: Assessment of cardiac preload and extravascular lung water by single transpulmonary thermodilution Intensive Care Med 2000, 26:180-187 Sakka SG, Klein M, Reinhart...
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Báo cáo y học: "Measurement of ventilation and cardiac related impedance changes with electrical impedance tomography" potx
... impedance change in dependent and non-dependent lung regions The sum of ΔZQ and ΔZV and ΔZV/ΔZQ in dependent and non-dependent regions for supine, prone, left and right lateral position (mean and confidence ... Australia 2Institute of Health and Biomedical Innovation, Queensland University of Technology, 96/110 Victoria Park Road, Kelvin Grove, Queensland 4059, Australia 3Paediatric and Neonatal Intensive ... the method described was suitable for filtering and separating regional ventilation and perfusion related impedance changes The regional distributions of ΔZV, ΔZQ and ΔZV/ΔZQ were examined in...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 1
... Hence, quantification of a wide range of potential chemical compounds in biological fluids and tissues may lead to proper understanding of the origin and progress of a disease For detection and quantification ... environmental contaminants along with biologically relevant molecules is an essential prerequisite for monitoring and understanding biological processes Precise quantification of xenobiotics in biosamples ... example, burning of plastics and certain fertilizers forms dioxin, a xenobiotic toxin, so pervasive and it can be found in nearly every human being [3] Many inorganic species may also be of particular...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 2
... place during the handling of body fluids All body fluids and solvents used in this project were decontaminated according to standard biohazard disposal protocols 26 Chapter 2.3.3 GC-MS Analyses ... systems of both female and male animals and humans Over the past several decades, estrogens have received much attention due to their association with many types of human gynaecological cancer [1] In ... Therefore, determining the level of estrogens in these body fluids would be very important for the study of their roles in the carcinogenesis of ovarian and breast cancer The role of estrogen in the progression...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 3
... C min−1 to 300◦C and held for OCP standards and samples were analyzed in selective ion monitoring (SIM) mode with a detector voltage of 1.5 kV and a scan range of m/z 50 to 500 3.5.2 HPLC analysis ... with respect to their levels in benign and malignant tumor cyst fluids This analysis helps to understand the influence of potential carcinogens in malignant transformation of ovarian tumors and its ... total of sixty prominent carcinogens were profiled in cyst fluids Cyst fluids of the ovary were collected from the subjects with benign (control) and malignant ovarian (study) lesions and were analyzed...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 4
... exposure and diseases or development abnormalities and to distinguish between exposed and control groups The data obtained from contaminant profiling of body fluids, especially tumor cyst fluids, ... supporting evidence pertaining to the tumor etiology to some extent Unfortunately, the analysis of PCBs and their metabolites in biological fluid and tissue samples involves complex, and time -and ... They play an important role and can influence the results provided by the instrumental techniques in quantitative determination, which is the final step of the analysis Classical methods use relatively...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 5
... variations in trace elemental concentrations between benign and malignant human ovarian cyst fluid specimens and to understand the effects of altered homeostasis of these elements in the etiology of ... benign and malignant ovarian cyst fluids are good source of information about tumor marker and other compounds which are associated with the origin and prognosis of tumor Thus, ovarian cyst fluid analysis ... Consequently, whole blood, serum and plasma have been used in biological research for the determination of trace metal status of individuals and groups [9-11] Nonetheless, in the case of ovarian...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 6
... at 10ºC min-1 to 300ºC (held for min) A standard solution of µg L-1 each analyte was initially analyzed in scan mode (at m/z range between 50 and 500) to identify the retention times and peak ... (i.e sample and derivatization agent were retained in the injection port for min) Since the injection port is heated to high temperature (300ºC) and pressurized, the holding time of would allow ... discarded) Using the same syringe, µL of BSTFA was withdrawn, making the total volume in the GC syringe µL The entire µL was injected into the GC-MS for injection port derivatiztion and analysis...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 7
... endorepellin can also attach to endostatin (another matrixderived inhibitor of angiogenesis that had been tested in clinical trials) and work against its anti-angiogenic effects [3] The superior anti-angiogenic ... regulation of proliferation and apoptosis, in turn leading to an altered protein expression and modification [12] Alterations in protein levels can be detected not only in the cancer cells, but also in ... Enhanced resolution of low molecular weight (MW) protein bands was obtained by preparing 12% resolving gel and increasing the final sample loading volume to 15 µl Table 7.2 Composition of mini-size...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 8
... complete profile of environmental organic, inorganic and steroid chemicals in cyst fluids helpful to understand their synergic effect on the malignant transformation and etiologic research of cancer ... important tumor fluids and tissues, the choice of analytes should be pertinent to the tumor, such as 164 Chapter carcinogens and EDCs Most of the analytes chosen are abundant in the environment, and ... factors in detail and other internal factors such as estrogens and endorepellin protein to distinguish benign and malignant stage ovarian tumor 166 Chapter The following conclusions can be drawn...
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INTERNATIONAL STANDARD Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp docx
... intended for human consumption and the feeding of animals; environmental samples in the area of food production and food handling WARNING — The method may not recover all Salmonella Typhi and ... specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing ... harmonization of test methods cannot be immediate, and for certain groups of products International Standards and/ or national standards may already exist that not comply with this horizontal method...
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Báo cáo " Research on the effect of urban expansion on agricultural land in Ho Chi Minh City by using remote sensing method " ppt
... applied and has been recognized as a powerful and effective tool in detecting urban land use and land cover change [4]. GIS technology provides a flexible environment for entering, analyzing ... cannot be stopped, with proper management and planning it can be directed in a desirable and sustainable way, protecting fertile agricultural land and ecological areas, creating green belt for the city. ... classification in an urban area Photogrammetric Engineering and Remote Sensing 61 (1995) 993. [5] Institute of Economic Research and Department of Culture and Information of Ho Chi Minh ...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25