mass spectrometry
Tài liệu Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry pdf
... Commun. Mass Spectrom. 2008; 22: 2993–2998DOI: 10.1002/rcm2994 L. Zhu et al.Real-time, on-line monitoring of organic chemicalreactions using extractive electrospray ionization tandem mass spectrometry Liang ... fast response. Mass spec-trometry-based methods are of particular interest for the on-line analysis of reactions,1due to their high sensitivity andhigh specificity. Tandem mass spectrometry ... organicchemistry.2,3Although direct infusion electrospray ioniz-ation spectrometry (ESI-MS)4–9and membrane introduction mass spectrometry (MIMS)10–12are gaining popularity in thisfield, both...
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Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx
... Proteome analysisof human colon cancer by two-dimensional differencegel electrophoresis and mass spectrometry. Proteomics4, 793–811.5 Roblick UJ, Hirschberg D, Habermann JK, PalmbergC, Becker ... colorectal cancer by com-bining two-dimensional polyacrylamide gel electrophore-sis with a strictly mass spectrometry- based approach fordata analysis. Mol Cell Proteomics 5, 2092–2101.9 Kim H, Kang ... eukaryotic organisms. Protein Sci 7,1029–1038.12 Wu CC & Yates JR III (2003) The application of mass spectrometry to membrane proteomics. Nat Biotechnol21, 262–267.13 Bisle B, Schmidt A, Scheibe...
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Tài liệu Báo cáo khoa học: A functional polymorphism of apolipoprotein C1 detected by mass spectrometry docx
... peptide mass fingerprintfrom MALDI-TOF MS showed m ⁄ z values corres-ponding to all eight theoretical peptides plus thepeptide of the truncated protein (residues 1–13,TPDVSSALDKLKE, theoretical mass ... reflectron mode and in aQSTAR o-MALDI mass spectrometer (Applied BiosystemsInc., Bellarica, CA, USA). MS ⁄ MS analysis was conductedwith the QSTAR o-MALDI mass spectrometer. The TOFregion acceleration ... National Hemophilia Clinical Fellow-ship Award. KM is a Bush Medical Fellow (BushFoundation). The mass spectrometry and parts of thegel electrophoresis were conducted with the assistanceof Mr Thomas...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... MS. The measured molecular mass of thepurified product was 11 799.70 ± 2, which concurs closelywith activated (Pyr)-ONC (M23L), which has a theoreticalmolecular mass of 11 801.60.Contribution ... soon as thesamples were dry, the probe was transferred into the mass spectrometer so that spectra could be acquired immediately.The mass spectra for the same samples were acquired again,24 h and ... of the peaks corresponding toGln-ONC (M23L) (theoretical molecular mass 11 818.87)and Pyr-ONC (M23L) (theoretical molecular mass 11 801.60), and expressing it as the fraction of hydrolyzed(Met1)-ONC...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... com-bined MS/MS of singly and doubly charged molecular ions innegative-ion electrospray mass spectrometry. J. Am. Soc. Mass Spectrom. 13, 670–679.15. Domon, B. & Costello, C.E. (1988) A systematic ... °Cat10°CÆmin)1.ES-MSNegative-ion ES-MS and CID MS/MS were carried out ona Micromass (Manchester, UK) Q-Tof mass spectrometer.Nitrogen was used as desolvation and nebuliser gas at a flowrate ... contaminant in DFiLNO as theprecursor (B).1176 H. Kogelberg et al.(Eur. J. Biochem. 271) Ó FEBS 2004 mass spectrometry and1H-/13C-nuclear magnetic resonancespectroscopy. Evidence for a new core...
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Tài liệu Báo cáo khoa học: Si-face stereospecificity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry ppt
... stereospecificity at C5 of coenzyme F420for F420H2oxidase from methanogenic Archaea as determined by mass spectrometry Henning Seedorf1,Jo¨rg Kahnt1, Antonio J. Pierik2and Rudolf K. Thauer11 ... nicotinamideadenine dinucleotide-linked oxidoreductases: electronimpact and field desorption mass spectrometry. AnalBiochem 101, 413–420.22 Warkentin E, Mamat B, Sordel-Klippert M, Wicke M,Thauer ... (5R)-[5-2H1]F420H2to [5-2H]F420as to be expected for aSi-face-specific enzyme. The finding that the 775 Da mass peak in Fig. 2Cb was much lower than inFig. 2Bb is probably due to the fact that reductionof...
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TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx
... Techniques for the Analysis of Organic Chemicals by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Petrochemical Authors Ed McCurdy & Don Potter Agilent Technologies ... Cheadle Royal Business Park Manchester, SK8 3GR UK Abstract Inductively Coupled Plasma Mass Spectrometry (ICP-MS) is used for the routine monitoring of trace and ultra-trace metal contaminants ... technique is available to any Agilent ICP-MS system fitted with the ShieldTorch System and a mass flow controller capable of adding oxygen to the plasma. In routine operation, automatic switching...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... soft ionization mass spectrometry. Int J Mass Spectrom 216, 1–27.54 Heck AJR & van den Heuvel RHH (2004) Investigationof intact protein complexes by mass spectrometry. Mass Spectrom Rev 23, ... (1998)Fourier transform ion cyclotron resonance mass spec-trometry: a primer. Mass Spectrom Rev 17, 1–35.56 Smith RD (2000) Evolution of ESI -mass spectrometry and Fourier transform ion cyclotron ... interconversion ofthe two most common frequency-to -mass calibrationfunctions for Fourier transform ion cyclotron resonance mass spectrometry. Int J Mass Spectrom 195 ⁄ 196, 591–598.83 Otwinowski...
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Báo cáo khoa học: Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry pptx
... least reliable mass isotopomer fractionswere considered in the flux analysis.DiscussionIn the work described, we examined the extent to which mass isotopomer measurements of biomass hydrolysates,after ... flux networks usingstable isotopes and mass spectrometry Maria I. Klapa*, Juan-Carlos Aon† and Gregory StephanopoulosDepartment of Chemical Engineering, Massachusetts Institute of Technology, ... M.I. (2001) High resolution metabolic flux quantificationusing stable isotopes and mass spectrometry. PhD Thesis,Massachusetts Institute of Technology, Cambridge, MA, USA.8. Wiechert, W., Mollney,...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... mass spectrometry forprotein interaction analysis: application to a calmodu-lin ⁄ target peptide complex. Anal Chem 77, 495–503.19 Tang XT, Munske GR, Siems WF & Bruce JE (2005) Mass spectrometry ... stored at )20 °C until MSanalysis.High mass MALDI-TOF MSHigh -mass MALDI-TOF mass spectra of the intact proteincomplexes were obtained using a MALDI-TOF mass spec-trometer (Voyager DE STR; Applied ... 000 98 000 132 000 166 000 200 000Intensity (%)100500 Mass (m/z) Mass (m/z)CountsCountsCountsABCFig. 2. High -mass MALDI-TOF mass spectra of the products generated upon cross-linking....
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... nanoflow electrospray ionizationorthogonal time-of-flight mass spectrometer (MicromassLC-T; Waters, Manchester, UK) modified for high mass operation and operating in positive ion mode [32]. Tomonitor ... described previously [31]. Three independent values wereobtained for each strain.Macromolecular mass spectrometry MS studies were carried out in aqueous ammonium acetate(100 mm, pH 7.0). Kid:Kis ... These protein complexesare detected at different mass- to-charge ratios ( m ⁄ z),separated by differences in their time-of-flight insidethe mass analyser. Here, we use this new powerfultechnology...
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Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx
... derivative.ATACP-TEMethyl-malonyladductUnloadedformsMethyl-malonyladductsUnloadedformadduct33 20032 80032 40039 50039 600 39 70039 400 Mass (Da) Mass (Da)Fig. 3. The electrospray mass spectra pro-duced by the AT and ACP-TE fractionsderived from the extension ... Transform mass spectrometry: The polyketide module of yersiniabactinsynthetase. Biochemistry 42, 13393–13400.33 Hicks LM, O’Connor SE, Mazur MT, Walsh CT &Kelleher NL (2004) Mass spectrometric ... ionization Fourier-Transform mass spec-trometry. Nat Prod Rep 23, 893–918.35 Bumpus SB & Kelleher NL (2008) Accessing naturalproduct biosynthetic processes by mass spectrometry. Curr Opin Chem...
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Báo cáo khoa học: Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry doc
... transform mass spectrometry: The polyketide module of yersiniabactinsynthetase. Biochemistry 42, 13393–13400.17 Hicks LM, O’Connor SE, Mazur MT, Walsh CT &Kelleher NL (2004) Mass spectrometric ... identity of each fragment was obtained by mass mapping from the known amino acid sequence. Giventhe good mass accuracy (0.01%), for any particularobserved mass a match could normally be found witha ... forcatalytic and structural studies.In our work, ion trap mass spectrometry was used.Compared with the Fourier transform-ion cyclotronresonance (FT-ICR) mass spectrometer [16,17], the iontrap is less...
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DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf
... capillary column of a gas chromatography /mass spectrometry (GC/MS) system. Compounds eluting from the GC column are identified by comparingtheir measured mass spectra and retention times to reference ... quadrupole mass spectrometer. Table 8 lists accuracy and precision data from replicatedeterminations of method analytes in tap water using liquid-solid cartridgeextractions and the ion trap mass ... andoutput mass spectral data. The computer software must have thecapability of processing stored GC/MS data by recognizing a GC peakwithin any given retention time window, comparing the mass spectrumfrom...
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Ionization Methods in Organic Mass Spectrometry pot
... the right conditions. For such compounds, negative ion mass spectrometryis more efficient, sensitive and selective than positive-ion mass spectrometry. Negative ions can be produced by a number ... polar or basic compounds. permits the detection of high -mass compounds at mass- to-charge ratios that are easily determined by most mass spectrometers (m/z typically less than 2000 to 3000).. ... electron energy to be precisely controlled for negative-ion mass spectrometry, leading to more sensitive, selective, and reproducible mass spectra for environmentally important compounds.. efficient...
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