... of bacteriophageP 22 and the mechanism of capsid maturation. J. Mol. Biol. 28 7, 527 –538. 26 . Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of ... utilizing the principle of protein–dye binding. Anal. Biochem. 72, 24 8 25 4. 27 . Paladini, A.A. & Weber, G. (1981) Pressure-induced reversibledissociation of enolase. Biochemistry 20 , 25 87 25 93. 28 . ... measurements of WT MS2 as a function of urea concentration. To verify the dissociation and denaturation processes, we measured the light scattering ofthe particlesat 320 nm (d)andthespectralcenterofmassoftheparticles(s)....
... Journal 27 7 (20 10) 1319–1330 ª 20 10 The Authors Journal compilation ª 20 10 FEBS 1 325 The ubiquitin ligase Itch mediates the antiapoptoticactivity of epidermal growth factor by promoting the ubiquitylation ... test this hypothesis, we treatedHEK -29 3T cells with TRAIL and EGF in the presence of the JNK inhibitor SP600 125 or in control condi-tions (Fig. 3C). Although the presence ofthe inhibitorhad ... degradation B. A. Azakir et al.1 326 FEBS Journal 27 7 (20 10) 13191330 ê 20 10 The Authors Journal compilation ê 20 10 FEBS conditions [29 ]. Treatment of HEK -29 3T cells withTRAIL is known to induce...
... notunderstand the “rules ofthe game” the way the United States does.That is one interpretation of both the crude Chinese nuclear threatsover the Taiwan crisis and Chinese views ofthe U.S. bombing of the Chinese ... confronta-tion between the Soviet Union and the United States was the centralreality in world politics. With the end ofthe Cold War and the disso-lution ofthe Soviet Union, the world continues ... result of agreementswith the United States and unilateral actions in the aftermath of the Cold War, and partly because ofthe state ofthe Russian economy.Even if Russia had not ratified the Strategic...
... super-position of PhyAsr with the open P-loop (green) (Protein Data Bank:1U24) and with Cys2 52 fully oxidized (gray) (Protein Data Bank:2PT0).AR258C2 52 BR258C2 52 Fig. 1. (A) Conformation ofthe P-loop ... respectively), again indicating that the open P-loop conformation was a result of oxidation of the cysteine.R258OCS 25 2AR258OCS2 52 BFig. 2. (A) The P-loop is observed in the catalytically inactive, ... A˚contact with Od 2ofthe cysteine sulfonic acid. In addi-tion to these contacts, there is a 1. 82 A˚contactbetween Gly255 Caand Od 2ofthe oxidized cysteine.To further understand the structural...
... 138 22 43 ± 42 319 ± 78 833 ± 53Glucose-LDL + Cu 2+ 29 82 ± 111 935 ± 116 21 46 ± 29 27 9 ± 57 764 ± 23 Formation of lipid-laden cells by glycated LDL B. E. Brown et al.15 32 FEBS Journal 27 4 (20 07) ... it may be the nat-ure ofthe products arising from glycation, rather thanpurely the loss ofthe parent amino acid, which is the key factor in terms of receptor recognition [29 ]. Thereare no ... diabetes [23 ,24 ], although the concentra-tions of these materials present in the artery wall, andin atherosclerotic lesions, are unknown. The role of glycation and the two facets of glycoxida-tion...
... lines are the fit of the model to the data.C. A. Salgueiro et al. Thermodynamic parameters in ligated proteinsFEBS Journal 27 2 (20 05) 22 51 22 60 ª 20 05 FEBS 22 53 of those measured for the isolated ... al. 22 54 FEBS Journal 27 2 (20 05) 22 5 122 60 ê 20 05 FEBS stages of oxidation the pH was adjusted inside the an-aerobic glove box with argon circulation to avoid the reoxidation ofthe sample. The ... C. A. Salgueiro et al. 22 56 FEBS Journal 27 2 (20 05) 22 5 122 60 ê 20 05 FEBS Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentreredox...
... reductase. J Biol Chem 27 8, 43973–439 82. 22 Kohli RM & Massey V (1998) The oxidative half-reac-tion of Old Yellow Enzyme. The role of tyrosine 196.J Biol Chem 27 3 , 327 63– 327 70. 23 French CE & ... than the reactive C1-C2 bond inclose proximity ofthe N5 atom. A putative flippingmotion ofthe progesterone molecule along an axisparallel to the FMN plane aligns the C1-C2 with the plane ofthe ... reductaseFEBS Journal 27 2 (20 05) 46604671 ê 20 05 FEBS 4671 Stopped-flow studies ofthe oxidative half-reaction of Y186F PETN reductase (reduced at the2 electronlevel with dithionite) with 2- cyclohexen-1-one...
... deviation.(Received 3 September 20 02, revised 9 December 20 02, accepted 20 December 20 02) Eur. J. Biochem. 27 0, 849–860 (20 03) Ó FEBS 20 03 doi:10.1046/j.14 32- 1033 .20 03.03441.x and (b) suggested the same overall ... 5B,b). The first turn involvesresidues Asp1 and Arg2 and brings the N-terminus of the peptide in spatial proximity to the C-terminus of the peptide. It was suggested that the stabilization of such ... [18],antiparallel pleated sheet [19 ,20 ], c-turn [19], random coil [21 ,22 ], inverse c-turn [23 ], side chain ring cluster [24 ], andother structures [25 29 ]. It is evident that several of the reported models are...
... getting the most from your money.80 /20 ruleKeep the 80 /20 rule in mind: 80% of your business comes from 20 % of your customers. (Koch, 1998). Getting to know the names and preferences of this ... ShoppersElectronically or the Old Fashioned Way Market newsletters are a great method of reminding your shoppers of why they love the market. The trend in this type of communication is the e-newsletter ... MGIOA.1marketing the market! 2 M a r k e t i n g t h eM a r k e t“Marketing is the whole business, taken from the customer’s point of view.” - Peter DruckerComing TogetherHow can the market,...
... 1.6-fold(0.047)Caspase-1activation40.4 ± 3.6 90 .2 ± 11.1 (2. 2-fold)0.00 02 57 .2 ± 10.4 0.06 1.6-fold(0. 02) Caspase-3activation1.5 ± 0.3 10.8 ± 2 (7 .2- fold)0.001 5.1 ± 2. 2(3.4-fold)0.048 2. 1-fold(0.03)Role of HIPPI as a ... (DOOPID: 9 425 2893, ) 24 5 to ) 25 3). The motif sequences of the caspase-3 gene (5Â-AAAGAGATG-3Â, ) 828 to) 820 ) and the caspase-7 gene (5Â-AAAGACATA-3Â,) 24 5 to ) 25 3) were present in the positive ... at the last base, G to A, abolished the interaction of HIPPI, so the last motif would notinteract with HIPPI. The other two motifs might be the target of HIPPI. In the upstream sequence of the Table...
... Dynamics of a CDase in the oligomeric stateFEBS Journal 27 3 (20 06) 109 121 ê 20 05 The Authors Journal compilation ê 20 05 FEBS 111 the intrinsic capability ofthe N- and C-terminalregions ofthe ... iM11 v q0ịx 2 r 2 r 2 0=2RTwhere i denotes the number of protomers per oligomer; Ai, the absorbance ofthe corresponding species;v, the partialspecific volume ofthe protein (calculated ... far-UV spectral region (190 25 0 nm)using a cell with a 0.1 cm path length, at 25 °C [23 ]. The width ofthe spectral band was 2 nm, and the time constantwas 2 s at 25 °C. The data were expressed...
... is the information gathered for the own use ofthe researcher and never gather before. The advantage ofthe primary data compared to secondary data are the information gathered is best suit the ... 12 1.8. Structure ofthe research Chapter 1: Introduction This chapter presents the preview ofthe problem of research, some concepts, nature and purpose of research. Chapter 2: Conceptual ... addition, they are aware 13 2. LITERATURE REVIEW 2. 1. The evolution ofthe CSR concepts What is CSR all about? Originally, the world witnessed the emergence ofthe modern definition of CSR...