local molecular mechanisms involved in human hyperostotic spine disorder pathological ossification in spinal ligament
Characterization of the molecular mechanisms involved in ethionamide activation in mycobacteria
... wall proteins, lipoproteins and secretion systems; 3, macrophage-interacting proteins; 4, protein kinases; 5, proteases; 6, metal-transporter proteins; 7, gene expression regulators including two ... roles in INH resistance remains unascertained in a large proportion of these genes (119) Approximately 10-25% of INHR strains not contain mutations in any of the known gene targets for INH resistance, ... actinobacteria, mycolic acids consisting of βhydroxyl fatty acids with long α-alkyl side chains line the inner layer of the mycomembrane, covalently linked to arabinogalactan in the standard inner...
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Molecular mechanisms involved in the specification of embryonic ectoderm in the zebrafish embryo
... amino-terminal extracellular Wnt binding domain and the 18 19 carboxy-terminal PDZ-binding domain of the Fz protein are known to be essential for Fz function Deletion of the PDZ-binding domain ... GSK3β is a serine/threonine kinase involved in insulin, growth factor and Wnt signalling In the absence of Wnt signalling, GSK3β is recruited to the APC complex via interaction with Axin, where it ... Lin, 2004) Recently, it was demonstrated that Axin also plays an important role in a JNK signalling pathway by utilizing discriminatory domains for its distinct roles in the both the β-catenin...
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Báo cáo y học: "Variations in the transcriptome of Alzheimer''''s disease reveal molecular networks involved in cardiovascular diseases" potx
... Trottier S, Epelbaum J: The proprotein convertase PC2 is involved in the maturation of prosomatostatin to somatostatin-14 but not in the somatostatin deficit in Alzheimer's disease Neuroscience ... located in module Plasminogen activator inhibitor type (PAI-1) proteins inhibit plasminogen activators [44] Therefore, if the level of PAI-1 is high in the AD affected samples, plasminogen activators ... are being inhibited, resulting in decreased levels of mature BDNF Interestingly, the expression levels of TrkB and PAI-1 were elevated in the AD samples However, TrkB is downregulated following...
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A functional genomics approach for elucidation of novel mechanisms involved in GnRH regulation of the gonadotropins
... C domain is the DNA-binding domain, which consists of two zinc-finger-like motifs The D domain is a variable hinge The multifunctional C-terminal (domain E) is the ligand-dependent domain (LBD), ... Hypothalamus-pituitary-adrenal Io Ionomycin IP3 Inositol 1,4,5 triphosphate JNK C-Jun NH2-terminal kinase LB Luria Bertani LH Luteinizing hormone LHR Luteinizing hormone receptor LHβ Luteinizing hormone β-subunit ... 115 4.3 Calcineurin is involved in GnRH-stimulated human αGSU promoter activity 122 4.4 The role of calcineurin in GnRH-mediated derepression of the FSHβ gene in the immature...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
... ethanol-induced mucosal injury in the upper gastrointestinal tract leading to increase in the permeability of the gut mucosa to endotoxins [43, 44] These factors are involved in ethanol-induced ... Interestingly, HMGN3 has been found to be associated with resistance against anticancer drugs including vinblastine, topotecan, paclitaxel and doxorubincin in human hepatocellular carcinoma derived ... NM_014393 ▲ 1.52 regulation of transcription protein binding double-stranded RNA binding DNA binding ▼ 5.02 protein binding NM_001149 NM_004242 ▲- increased ▼- decreased Validation of microarray...
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Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological significance docx
... kinetic folding intermediates of apomyoglobin J Mol Biol 355, 139–156 Jamin M & Baldwin RL (1996) Refolding and unfolding kinetics of the equilibrium folding intermediate of apomyoglobin Nat Struct ... binding in human neuroglobin and cytoglobin: molecular mechanisms and physiological significance J Biol Chem 279, 44417–44426 11 Burmester T & Hankeln T (2004) Neuroglobin: a respiratory protein ... binding affinity of human neuroglobin and cytoglobin J Biol Chem 278, 51713–51721 Trent JT III, Watts RA & Hargrove MS (2001) Human neuroglobin, a hexacoordinate hemoglobin that reversibly binds...
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Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc
... (S)-cheilanthifoline, not (S)-nandinine [7,30,31] If we assume that (S)-nandinine is produced in E californica, there may be no need for a catalyst from (S)-nandinine to (S)-stylopine However, nandinine could ... (S)-nandinine from (S)-scoulerine in vitro; indeed, CYP719A3 converted (S)-scoulerine to (S)-nandinine with high affinity (Km ¼ 0.54 lm) (Fig 6) Previous reports have shown that (S)-stylopine is ... nandinine based on its total m ⁄ z and fragment ion patterns (data not shown) Nandinine is a scoulerine derivative with the methylenedioxy bridge in ring A (2,3-position), whereas cheilanthifoline...
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Báo cáo Y học: Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt
... A., Inoue, K., Hiraoka, N., Ikeshiro, Y., Kushiro, T., Shibuya, M & Ebizuka, Y (2001) Cloning and characterization of a cDNA encoding b-amyrin synthase involved in glycyrrhizin and soyasaponin ... Ebizuka, Y (1998) Molecular cloning of cDNA from Panax ginseng The isogene that encodes b-amyrin synthase In Towards Natural Medicine Research in the 21st Century, Excerpta Medica International Congress ... were maintained in a 300-mL Erlenmeyer flask containing 60 mL Linsmaier and Skoog medium [23], supplemented with 100 nM 1-naphthaleneacetic acid in the dark at 25 8C, and subcultured at intervals...
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Báo cáo khoa học: Molecular mechanisms regulating molting in a crustacean Halagowder Devaraj and Ayithan Natarajan ppt
... mitogen-activated protein kinase pathway is involved in a number of cellular changes inducing growth and differentiation in human Schwann cells [7] In cardiac myocytes, protein kinase C generates ... cell cycling in human Schwann cells Receptor tyrosine kinases play a central role in transducing the external signals across cell membranes into the intracellular signaling systems, which in turn ... responses in human Schwann cells [7] Receptor tyrosine kinases transduce the signals via extracellular signal-regulated kinases (ERKs), the serine ⁄ threonine protein kinases belonging to the...
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Báo cáo khóa học: Phospholipase C, protein kinase C, Ca 2+ /calmodulin-dependent protein kinase II, and redox state are involved in epigallocatechin gallate-induced phospholipase D activation in human astroglioma cells ppt
... protein kinase C, Ca2+/calmodulin-dependent protein kinase II, and tyrosine phosphorylation are involved in carbachol-induced phospholipase D activation in Chinese hamster ovary cells expressing ... To determine whether PLC activity or G-protein-mediated signaling was involved in EGCG-induced PLD activation in U87 cells, we examined the effects of pertussis toxin and the phosphoinositide-specific ... pathway in many cell types [36], and the inhibitor attenuated EGCG-induced increases in PLD activity, PLC probably regulates PLD through stimulation of this kinase Interestingly, the increase in PLD...
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Báo cáo khoa học: Cell death-inducing DFF45-like effector, a lipid droplet-associated protein, might be involved in the differentiation of human adipocytes pdf
... particularly during embryonic and fetal life [3] Cell death-inducing DFF45-like effector (CIDE) proteins, including cell death-inducing DFF45-like effector A (CIDEA), cell death-inducing DFF45-like ... al molecules involved in adipocyte differentiation and lipid droplet formation on day 14 after induction As lipid droplet-targeting proteins, perilipin and fatty acid-binding protein (FABP) are ... plasmid expressing enhanced green fluorescent protein (EGFP)-tagged adipophilin (also named perilipin-2) was co-transfected into COS-7 cells with a plasmid containing hemagglutinin (HA)-tagged...
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Báo cáo khoa học: Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme pptx
... both being in dimer form in low protein concentrations and being reconstituted into tetramers in high protein concentrations In contrast to the E59Q enzyme, the E59D enzyme has a predominantly ... crystal structure of human m-NAD-ME in complex with fumarate has been determined, the binding network of fumarate in the enzyme has become clear In the fumarate-binding pocket, two arginyl residues, ... and polymers in the range of protein concentrations used (Fig 3GI), indicating that the quaternary stability in the dimer interface is greatly changed by shortening one carbon side chain of Glu59...
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Báo cáo khoa học: Protein kinase Ch activity is involved in the 2,3,7,8tetrachlorodibenzo-p-dioxin-induced signal transduction pathway leading to apoptosis in L-MAT, a human lymphoblastic T-cell line potx
... [9,10] In previous studies, we attested that AhR cannot be involved in TCDD-induced apoptosis in the human T-cell line, L-MAT, because AhR does not exist in L-MAT [7,8] In view of our previous findings ... of PKCh in the TCDD-induced AhR-independent signal transduction mechanism involved in L-MAT cell apoptosis Results Inhibition of TCDD-induced L-MAT cell apoptosis by rottlerin, an nPKC inhibitor ... Biomedicals Inc., Irvine, CA, USA), pH 7.4, containing 5% fetal bovine serum, 100 IUÆmL)1 penicillin, and 0.1% (v ⁄ v) streptomycin at 37 °C in 95% air and 5% CO2 Jurkat T cells were maintained under...
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Báo cáo khoa học: Sp1 and Sp3 are involved in up-regulation of human deoxyribonuclease II transcription during differentiation of HL-60 cells pptx
... Sp1 and Sp3 is involved in the PMA-mediated up-regulation of DNase II expression In addition to an increase in protein levels, Sp1 may regulate gene expression by changing DNA binding affinity or ... mediate the PMA-induced transcription of lysosomal acid lipase and acid sphingomyelinase in THP-1 cells In this study, we show that Sp1 is involved in PMA-induced expression of DNase II in HL-60 cells ... anti-Sp3 IgG, indicating they contain proteins other than Sp1 or Sp3 It is not clear whether these unknown factors interact with Sp1 or Sp3, facilitating their binding to GC-I and enhancing their...
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Báo cáo khoa học: Lipid droplet and milk lipid globule membrane associated placental protein 17b (PP17b) is involved in apoptotic and differentiation processes of human epithelial cervical carcinoma cells pptx
... factor binding sites clustered in the vicinity of the Inr might serve in transcription initiation (Fig 1) Analysis of the 1.5-kb 5¢ flanking region, attempting to get further insight into the ... the staining for these proteins could be detected in the cytosol (Fig 4A) In lipid-loaded cells, amounts of PP17a, PP17b and PP17c were increased in the cytosol fraction, and in parallel an intense ... transcription factor binding sites involved in apoptosis and differentiation were localized in the PP17 gene promoter Using well-characterized apoptosis and differentiation models, induction of PP17...
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Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
... to amino acids in the L-domain (residues N204, D205, N231, E256 and E290) and the S-domain (residues T168 and K169) (Fig 1B) To identify the contact residues involved in the initial binding of ... DS is in keeping with that an increase in protein dynamics plays a dominant role in the interaction, with large scale domain movement and cleft closure (desolvation) as well as changes in peptide ... fluorescence enhancement induced by Glc binding; (b) to identify the active site residues involved in the binding of Glc to the super-open state (Fig 1C) of this two domain [large (L) and small...
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Báo cáo khoa học: Molecular mechanisms in successful peripheral regeneration pot
... Repair of spinal cord injuries: where are we, where are we going? Spinal Cord 40, 615–623 Schwab ME (2002) Increasing plasticity and functional recovery of the lesioned spinal cord Prog Brain Res ... central axonal sprouting in the facial motor nucleus model [87], enhance functional recovery after spinal cord injury [106], but eliminate the conditional injury-induced spinal axon regeneration ... could indicate insufficient BDNF ⁄ GDNF-mediated support following chronic axotomy These findings are in keeping with 2631 Molecular mechanism in regeneration the strong, 50% reduction in the enduring...
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Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx
... instructions provided by the manufacturer (Invitrogen) The strain harbouring the correct construction was incubated in selecting liquid medium according to Invitrogen recommendations, until the D600 ... in activity However, in the BEAT sequence, which encodes an enzyme involved in scent production, glycine was substituted by methionine in the conserved triad H-x-x-x-DG In yeast AATs [15], only ... is probably not involved in its expression Search for alcohol acyl-transferase activity of CM-AAT1 and CM-AAT2 recombinant proteins None of the recombinant proteins produced in Escherichia coli...
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Báo cáo hóa học: " Exploring the molecular mechanisms underlying the potentiation of exogenous growth hormone on alcohol-induced fatty liver diseases in mice" ppt
... are indicated in bold/underlined) from the template of a pUC19 plasmid DNA containing GH1 (Xinxiang Medical University, Xinxiang, Henan Province, China) The 677-bp GH1 DNA fragment (including ... and molecular mechanisms of GH on AFLD Viral vectors can induce longerlasting effects than recombinant protein administration and thus avoid the inconvenience of repetitive subcutaneous injections ... least in part, by turning on the hepatic adiponectin-SIRT1-AMPK signaling system and related signaling pathways to ameliorate alcoholinduced impairments in the signaling pathways controlling lipid...
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Báo cáo khoa học: "The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells" doc
... The underlying mechanisms are, however, not fully understood Arsenic exerts its effect by inhibiting the activities of several enzymes, especially those involved in cellular signaling pathways ... change into a pro-apoptotic phenotype following exposure to arsenic trioxide Also Bax activation was involved in arsenic trioxide-induced conformational change of Bcl-2 by immunostaining SGC7901 ... trioxide-treated SGC7901 cells The cells were stained using DAPI staining B Untreated SGC7901 cells stained by anti-Bcl-2 N terminus antibody C SGC7901 cell stained by anti-Bcl-2 BH3 and anti-Bax(6A7)...
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