... region and b strand 5A of PAI-1 and studied their effect on the transition to latent and substrate forms and on the stabilizing effect of vitronectin, a flexible joint region-binding a cofactor known ... were: wild-type, 48.6 and 65.2 min; K335A, 78.9 and 41.8 min; S53A, 69.4 and 109.9 min; S53A/K335A, 99.2 and 43.5 min; G56S/Q334H, 5.3 and 59.8 min; G56S/Q334H/K335A, 26.5 and 35.1 A summary of ... not identical to that giving an increased rate of latency transition Previously, Triton X-100 was found to induce substrate behaviour in nonglycosylated PAI-1 at and 37 8C [37] andto induce substrate...
... factor present in the cell extracts of both C difficile and E coli Apparently, this factor is lost during purification and limiting when cell-free extracts are used to restore activity (P I Andrei ... shown in bold letters together with the C-terminal end of HpdB and the N-terminal of HpdA sequences of the resulting proteins (MSQS to MASS in HpdB, MRKH to MGKH in HpdC and MSSQ to MASQ in HpdA) ... D.W (1989) Molecular cloning A laboratory Manual, edn, Cold spring harbor laboratory press, New York Parker, B & Marinus, M.G (1988) A simple and rapid method to obtain substitution mutations...
... Litsea glutinosa were shown to exhibit significant cytotoxic activity against human Hela cell lines in vitro Chemical examination of the BuOH extract of the leaves and twigs of Litsea glutinosa ... H2, injector temperature (PTV) 250oC, detector temperature 260oC, temperature programmed 60o (2 hold) to 220o (10 hold) at 4oC/min GC/MS- An Agilent Technologies HP 6890 N Plus Chromatograph was ... oils known composition and also with standard substances [12-17] Experimental Results and discussion Source- Litsea glutinosa (Lour.) C B Rob (Lauraceae), is a shrub tree up to 7-10m high, growing...
... m NaOH for 10 Band density was analysed using quantity one densitometry software (Bio-Rad) and the intensity of each band was then normalized to the level of total NF-jB Because total p65–NF-jB ... the expression of proinflammatory cytokines and chemokines [e.g tumour necrosis factor a (TNFa), interleukin (IL)-1b, IL-6, monocyte chemoattractant protein-1 (MCP-1)] and the enzyme cyclooxgenase-2 ... containing triacylglycerol (TG) from palm, olive, corn and fish or trilinolein were prepared as described in Materials and Methods and the TG and total cholesterol (TC) content (lmolÆmL)1) was measured...
... Testis: Number and Distribution of Cases by Histology and AJCC Stage (SEER modified 5th Edition), Ages 20+, 12 SEER Areas, 1988-2001 AJCC Stage Total I II III Unknown/ Unstaged Histology Total Germ ... (20.7%) and the largest proportion of Stage III tumors (74.1%) Table 21.2: Cancer of the Testis: Number and Distribution of Cases by Histology and Age (20+), 12 SEER Areas, 1988-2001 Age (Years) Total ... I (no spread to lymph nodes or distant organs), Stage II (the cancer has spread to regional lymph nodes but not to lymph nodes in other parts of the body or to distant organs), and Stage III...
... ∞, and T is the homotopy operator which can be found in Also, from , we know that both Ms u and M# u are Ls -integrable 0-form s Differential forms, the Green’s operator, and maximal operators ... , Ding and Liu investigated singular integrals for the composition of the homotopy operator T and the projection operator H and established some inequalities for these composite operators with ... factors In paper 11 , they keep working on the same topic and derive global estimates for the singular integrals of these composite operators in δ-John domains The purpose of this paper is to...
... thickness had increased to 50 µm in beech and spruce, andto 70 µm in oak In beech and oak rootlets, the periderm consisted of only to cell layers close to the root tip but increased to 7–8 (beech) or ... upscaling process from laboratoryto field Root chemical and anatomical properties and even Lpr may be less important in controlling in situ water absorption than they are in a laboratory setup with ... sealed in plastic bags and transported to the laboratory Five roots were analysed for their diameter/distance relationship, three were used for anatomical investigation, and another three for...
... 150 mmHg and diastolic at 95 mmHg), and serum creatinine levels increased to 125 umol/l This was attributed to cisplatin renal toxicity, and then prednisolone decrease was continued In October 2004, ... after the surgery Prednisolone was then increased to mg/kg/day for month, and then decreased to 0.75 mg/kg/day, and then 0.5 mg/kg/day to allow for postoperative healing In May 2004, a CT scan showed ... with both a scrotectomy and an orchiectomy A staging work-up, with thoraco-abdominal CT scan and standard blood chemistry showed no abnormality The patient was then referred to our occupational...
... experiment Charcoal chips (approximately 100 g-wet and 15 g-dry) and medium water (300 mL) were put into a flask together, and purged with N2 gas to keep the anoxic condition All experiments were ... Dissolved oxygen concentrations and pH levels were measured by the Galvanic DO sensor (DO-24, TOA DKK, Japan) and the pH sensor (WM-50EG, TOA DKK, Japan) at the beginning and the end of the batch experiment ... Journal of Water and Environment Technology, Vol 8, No.4, 2010 The contribution ratio of PC1 and PC2 were 43.8% and 18.6%, respectively, and the accumulated contribution ratio for PC1 and PC2 was...
... where necessary The reaction was stopped by cooling in ice, and the samples were subjected to 15% SDS/PAGE The gels were dried and autoradiographed A total of 100 hand-dissected pairs of testes were ... GAL4-activator (AD), or GAL4-binding (BD) domains Besides, the ubiquitous Drosophila CK2 b subunit was cloned in both AD- and BD-vectors To assay interactions, different combinations of AD- and BDconstructs ... We are grateful to Dr C.V.C Glover for providing us with the pEV55Dmß plasmid and the anti-DmCK2a antiserum, andto Dr H.D Hoyle for providing the testis vector We would like to thank Prof N.B...
... transported proteins from the cytosol to their final destination in the chloroplast The Tic and Toc translocons are therefore perfectly situated to impose a regulatory control over incoming preproteins ... conditions lead to fast membrane binding and integration into the Tic complex Reducing conditions on the other hand lead to solubilization into the stroma and increased interaction with its other ... motor function Early chloroplast import studies demonstrated that cytoplasmically synthesized preproteins are imported into the organelle in an ATP-dependent process [23] By contrast to mitochondria,...
... GLUT8 in the absence of AP1 and AP2 In order to investigate the contribution of AP adaptors, most notably AP1 and AP2, to GLUT8 sorting we downregulated individual adaptor complex subunits GLUT8 ... internalization of this construct is too fast and efficient to be detected by this approach (similar to the antibody feeding in HeLa cells overexpressing GLUT8 and described above) and ⁄ or that its intracellular ... to an intracellular compartment and ⁄ or that AP2-dependent endocytosis occurs very rapidly Mutating the LL signal to AA in the TAC chimeric protein totally abolished sorting of the chimera to...
... (satisfactory quality studies provided clear statements on material and nutrients analysed, laboratoryand statistical methods and a clear definition of organic agricultural practices), and one ... organically and conventionally produced crops and livestock products are biologically plausible and most likely relate to differences in crop or animal management, and soil quality There is no good ... foodstuffs prior to reaching the plate of the consumer An understanding of the various factors that affect nutrient variability in crops and livestock products is important for the design and interpretation...
... transcription factors in the testis [12] Studies have established that TNF-a is a major cytokine produced and released by germ cells and that TNF-a receptors are found on Sertoli and Leydig cells ... factor-kappa B J Biol Chem 280, 244–252 31 Lysiak JJ (2004) The role of tumor necrosis factoralpha and interleukin-1 in the mammalian testis and their involvement in testicular torsion and autoimmune ... complex leads to the release of cytoplasmic NF-jB and nuclear relocation of NF-jB In an IKK-independent pathway, external stimuli, including hypoxia and genotoxic stresses, lead to NF-jB nuclear...
... medicine such as in bitter stomachic, skin diseases, and reported to have antitumor, insecticide, and cytotoxic activities The antimicrobial activities of coumarin constituents and the neuropharmacological ... medicine in bitter stomachic, skin diseases, and reported to have antitumor, insecticide and cytotoxic activities [3] The antimicrobial activities of coumarin constituents [4] and the neuropharmacological ... composition and antibacterial activity of the essential oils from Launaea resedifolia L Amar Zellagui1, Noureddine Gherraf*1, Segni Ladjel2 and Samir Hameurlaine2 Laboratory of Biomolecules and Plant...
... medicine such as in bitter stomachic, skin diseases, and reported to have antitumor, insecticide, and cytotoxic activities The antimicrobial activities of coumarin constituents and the neuropharmacological ... medicine in bitter stomachic, skin diseases, and reported to have antitumor, insecticide and cytotoxic activities [3] The antimicrobial activities of coumarin constituents [4] and the neuropharmacological ... composition and antibacterial activity of the essential oils from Launaea resedifolia L Amar Zellagui1, Noureddine Gherraf*1, Segni Ladjel2 and Samir Hameurlaine2 Laboratory of Biomolecules and Plant...
... by Gas chromatography/mass spectrometry Allured Publishing Corporation, Carol Stream, 13 Mouhssen L (2001) Methods to study the photochemistry and bioactivity of essential oils Phytother Res 18:435–448 ... (determined with reference to a homologous series of normal alkanes), and by comparison of their mass spectral fragmentation patterns with those reported in the literature [12] and stored on the MS library ... sulphate Diethyl ether was removed carefully and the essential oil was collected and stored at 4°C until analysis The oil yield was calculated relative to the dry matter 2.2 GC-MS analysis 2.4 Antibacterial...
... is standard and left to the reader Since functions in ᏸ p (Bn ) extend continuously to Bn , they are automatically in L1 (σ) [8, Remark, page 107] and since the quotients of these functions and ... equality to However, in the case, that p ∈ (0,1), Ꮾ p (Bn ) is the same as and norm-equivalent to ᏸ1− p (Bn ), whose compact composition operators are known (by a result due to J H Shapiro) to be ... {1,2 ,n} and z ∈ Bn , then Cφ is a bounded operator from ᏸ p (Bn ) to ᏸq (Bn ) (B) If Cφ is a bounded operator from ᏸ p (Bn ) to ᏸq (Bn ), then there is an M ≥ such that for all z ∈ Bn and u ∈...
... begin to produce seed; and at harvest, seed, and leaves, and stalks are rich in the flesh -and blood-forming element of plants In more humid countries plants delay the time of seed production and ... slightly increased price, and trusted to the increase in weight due to water absorption in more humid climates for their profits The time should be near at hand when grains and similar products should ... understand this principle send to the Middle West for seed corn, with the result that great crops of stalks and leaves with no ears are obtained The only safe rule for the dry-farmer to follow is to...