l opg and rank proteins by sf neutrophils from patients with ra
Báo cáo y học: "Pro-apoptotic Bid is required for the resolution of the effector phase of inflammatory arthritis" pdf
... H&E ankle sections were scored on a to scale for inflammation, with = normal, = minimal infiltration, = mild infiltration, = moderate infiltration, = marked infiltration, and = severe infiltration ... and B cells Figure ankles following transfer of K/BxN serum Bid-deficient mice develop a sustained and prolonged edema of the ankles following transfer of K/BxN serum Pooled serum (300 l) from ... suggest that while the local inflammatory milieu remains similar in Bid-/- and Wt mice, the systemic levels of IL-1β and KC are increased These elevated levels of IL-1β and/ or KC may lead to the...
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Báo cáo khoa học: Neptunium uptake by serum transferrin potx
... impossible and cation transfer inside the cell is disabled Consequently, comparison between the lobe conformation in the holo and Np form is also essential Experimental procedures Np(IV) stock solution ... been tested by EXAFS and further spectroscopic and theoretical investigations are needed to support this model The average Np–ligand distances are in agreement with comparable crystallographic structures ... plutonium and interactions of plutonium with macroorganisms and plants In Avances in Plutonium Chemistry 1967–2000) Darleane Hoffman, American Nuclear Society, La Grange Park, Il, USA Sun H, Li...
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báo cáo hóa học: " Astrogliosis is delayed in type 1 interleukin-1 receptor-null mice following a penetrating brain injury" pptx
... expected, IL-1β increased CP significantly in the astrocyte and mixed glial cultures Extracellular matrix molecules are independent of IL-1R1 (Fig and 6) Extracellular matrix (ECM) molecules play an ... mixed glial cultures, the level was very low in the cortical neuronal cultures and below the level of detection in the microglial cultures Altogether, these results suggest that brain cells are ... Biol 1989, 109(6 Pt 1):3063-3071 Donato R: S100: a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles Int J Biochem Cell...
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báo cáo hóa học: " Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury" potx
... model, an early, single dose of gelsolin can dramatically reduce mortality by ameliorating cerebral inflammatory lesions and apoptosis via acceleration of recruitment of monocytes and down-regulation ... collected and stored at -80°C until analysis for IL-2 by ELISA (ExCell Biology Inc., Shanghai, China) T cell proliferation was examined using a 3-(4, 5-dimethylthiazol -2-yl)- 2, 5-diphenyltetrazolium ... cord blood cells in a rat model of stroke Stem Cells Dev 2005, 14:595-604 20 Yin HL, Stossel TP: Control of cytoplasmic actin gel-sol transformation by gelsolin, a calcium-dependent regulatory...
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Báo cáo khoa học: "Mucosal mast cell-derived chondroitin sulphate levels in and worm expulsion from FcRγ-knockout mice following oral challenge with Strongyloides venezuelensis" doc
... challenge Fig Number of adult worms (mean ± SD) recovered from the small intestines of immune and control FcRγ-KO and WT mice challenged orally with 300 lung-recovered L3 of S venezuelensis and ... mean daily EPG of animals sacrificed on day 13 post challenge are presented in Fig All control WT and KO animals as well as all challenged KO mice persistently shed eggs in their faeces until the ... previously described [16] on day and 13 post challenge Also samples of gut washings as described below were collected on days and post challenge and analysed for mMCP-1 concentration by ELISA as...
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Báo cáo y học: " PCEP enhances IgA mucosal immune responses in mice following different immunization routes with influenza virus antigens" pdf
... provided by grants from the Krembil Foundation and the Bill and Melinda Gates Foundation through the Grand Challenges in Global Health Initiative The assistance from Barry Carroll and Animal Care ... into the vaginal, nasal, and lung cavities, respectively, and subsequently withdrawn for collection All samples were promptly centrifuged and the resulting supernatants were collected and assayed ... PCEP with influenza X:31 antigen when administered by parenteral (subcutaneous), and mucosal (intranasal, oral, and intrarectal) routes We show that, while PCEP has adjuvant activity in all routes...
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Báo cáo y học: "Early Reversal of experimental colitis disease activity in mice following administration of an adenoviral IL-10 vector" pps
... single inflammatory cell infiltration of crypts, slight monocyte and neutrophil infiltrate; a score of signifies loss of multiple epithelial cells (in patches), obvious flattening of the epithelia, ... interleukin-8 production in human neutrophils Blood 1994, 83:2678-2683 Willems F, Marchant A, Delville JP, Gerard C, Delvaux A, Velu T: Interleukin-10 inhibits B7 and intercellular adhesion molecule-1 ... several mechanisms including inhibition of the release of several inflammatory factors (IL-1, IL-6, IL-12, TNF-α, GM-CSF, GCSF), suppression of cell adhesive determinants (MHC class II molecule,...
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Báo cáo y học: " Pneumocystis murina colonization in immunocompetent surfactant protein A deficient mice following environmental exposure" pdf
... mice develop higher levels P murina infection than WT mice [24,25] Immunocompetent and CD4+ T-cell depleted KO mice also display delayed clearance following infection by intratracheal inoculation ... in an ELISA reader Isolation and Analysis of Pulmonary CD4+ and CD8+ T cells Cells were isolated and quantitated from the lungs as previously described [34] Briefly, lungs were removed and ground ... glass slides in PBS with 1% BSA and mononuclear cells were isolated from the homogenate on 40 to 70% Percoll gradients and enumerated on a Z2™ Coulter Counter® (Beckman Coulter, http://respiratory-research.com/content/10/1/10...
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Báo cáo y học: "Matrix metalloproteinase-8 deficiency increases joint inflammation and bone erosion in the K/BxN serum-transfer arthritis model" pptx
... of inflammation by IL-1b and other molecules like PTX3, induction of maturation and activation of osteoclasts by IL-1b and PROKR2, and enhanced inflammatory infiltrate by IL-1b and possibly PROKR2 ... synovial lining and moderate infiltration of the sublining; = thickening of synovial lining and marked infiltration; and = thickening of synovial lining and severe infiltration Cartilage damage ... of cartilage with minor loss of surface cartilage; = moderate cartilage abnormalities including loss of superficial cartilage and moderate multifocal chondrocyte loss; and = marked cartilage destruction...
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Báo cáo khoa học: Brain lipid composition in postnatal iron-induced motor behavior alterations following chronic neuroleptic administration in mice potx
... placed in a sound-proofed wooden box with 12 cm thick walls and front panels and a small double-glass window to allow observation; each box had a dimmed lighting Mice were killed by cervical ... Ethical Experiments on Laboratory Animals (license S93 ⁄ 92 and S77 ⁄ 94, Stockholm, Sweden) Chemicals and reagents Lipid standards of l- a-phosphatidylcholine (PC from egg yolk) and N-acyl-d-sphingosine-1-phosphocholine ... biochemically and behaviourally (apomorphineinduced activity) Moreover, neuroleptics may alter brain phospholipid metabolism and composition by regulating phospholipase A2 (PLA2) activity Possible effect...
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Báo cáo sinh học: "SERCA2a gene transfer improves electrocardiographic performance in aged mdx mice" pdf
... Mendell JR, Campbell K, Rodino-Klapac L, Sahenk Z, Shilling C, Lewis S, Bowles D, Gray S, Li C, Galloway G, Malik V, Coley B, Clark KR, Li J, Xiao X, Samulski J, McPhee SW, Samulski RJ, Walker ... is elevated at all ages J Neurol Sci 1991, 103:226-231 Alloatti G, Gallo MP, Penna C, Levi RC: Properties of cardiac cells from dystrophic mouse J Mol Cell Cardiol 1995, 27:1775-1779 Williams ... function in failing aortic-banded rat hearts by gene transfer of calcium cycling proteins J Mol Cell Cardiol 2007, 42:852-861 17 Kawase Y, Ly HQ, Prunier F, Lebeche D, Shi Y, Jin H, Hadri L, Yoneyama...
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báo cáo hóa học: " Boosting with intranasal dendrimeric Aβ1–15 but not Aβ1–15 peptide leads to an effective immune response following a single injection of Aβ1–40/42 in APP-tg mice" ppt
... nasal cavity Control mice received LT(R192G) alone All vaccinations were administered weekly for months Plasma and tissue collection Blood was collected from the tail bi-weekly and the plasma ... Pathologically there is localized deposition of cerebral β-amyloid (Aβ) protein, neuritic plaques, glial activation, neurofibrillary tangle formation, and neuronal loss [1] The cause of AD is still ... TJ, Lemere CA: Modulation of the humoral and cellular immune response in Abeta immunotherapy by the adjuvants monophosphoryl lipid A (MPL), cholera toxin B subunit (CTB) and E coli enterotoxin LT(R192G)...
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báo cáo hóa học: " Interleukin-1 receptor 1 knockout has no effect on amyloid deposition in Tg2576 mice and does not alter efficacy following Aβ immunotherapy" pot
... amyloid deposition in these mice [36,37] Another member of the IL-1 superfamily, IL-1 receptor antagonist (IL 1Ra) [38], is also synthesized and released in parallel to IL-1β, IL-1α, and IL-18 IL- 1Ra ... Green KN, LaFerla FM: A dynamic relationship between intracellular and extracellular pools of Abeta Am J Pathol 2006, 168(1):184-194 Wilcock DM, DiCarlo G, Henderson D, Jackson J, Clarke K, Ugen ... Dinarello CA: The IL-1 family and inflammatory diseases Clin Exp Rheumatol 2002, 20(5 Suppl 27):S1-13 Greenfeder SA, Nunes P, Kwee L, Labow M, Chizzonite RA, Ju G: Molecular cloning and characterization...
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báo cáo hóa học:" SERCA2a gene transfer improves electrocardiographic performance in aged mdx mice" docx
... Mendell JR, Campbell K, Rodino-Klapac L, Sahenk Z, Shilling C, Lewis S, Bowles D, Gray S, Li C, Galloway G, Malik V, Coley B, Clark KR, Li J, Xiao X, Samulski J, McPhee SW, Samulski RJ, Walker ... is elevated at all ages J Neurol Sci 1991, 103:226-231 Alloatti G, Gallo MP, Penna C, Levi RC: Properties of cardiac cells from dystrophic mouse J Mol Cell Cardiol 1995, 27:1775-1779 Williams ... function in failing aortic-banded rat hearts by gene transfer of calcium cycling proteins J Mol Cell Cardiol 2007, 42:852-861 17 Kawase Y, Ly HQ, Prunier F, Lebeche D, Shi Y, Jin H, Hadri L, Yoneyama...
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báo cáo hóa học:" Ovarian hyperstimulation syndrome and prophylactic human embryo cryopreservation: analysis of reproductive outcome following thawed embryo transfer" pdf
... interval between retrieval and thaw/transfer, number (and developmental stage) of embryos transferred (ET), and livebirth-delivery rate All data were tabulated as mean ± SD Patients were periodically ... present and morphologically normal at baseline for all patients at risk for OHSS All patients underwent ultrasound-guided transvaginal oocyte retrieval at our facility without incident; the average ... Villasante A, Pacheco A, Pau E, Ruiz A, Pellicer A, Garcia-Velasco JA: Soluble vascular endothelial-cadherin levels correlate with clinical and biological aspects of severe ovarian hyperstimulation...
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Báo cáo khoa học: "Serum immunoglobulin fused interferon-Ձ inhibited tumor growth in athymic mice bearing colon 26 adenocarcinoma cells" ppsx
... harvested from blood samples obtained from the mice The following parameters were assayed: total protein (TPROT), albumin (ALB), total bilirubin (TBILI), aspartate aminotransferase (AST), alanine ... at low levels in many tissues and cell types and reaches high concentrations in the brain and pituitary FGF2 has been implicated in a multitude of physiologic and pathologic processes, including ... ± C , with a relative humidity of 50 ± 20% and a 12 h light/dark cycle After days of tumor cell inoculation, the mice were grouped as follows: control, 10 × and 50 × IFN concentrations All the...
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Báo cáo y học: "A comparative study of the inhibitory effects of interleukin-1 receptor antagonist following administration as a recombinant protein or by gene transfer." pps
... at a density of × 105 cells per well in 24-well plates with ml DMEM supplemented with 10% FBS and 1% penicillin-streptomycin Engineered cell lines To generate a cell line that provided a source ... IL-1 stimulation, and IL- 1Ra concentration immediately before and 48 hours after IL-1stimulation PGE2/IL- 1Ra levels for cultures receiving the IL- 1Ra producing cells are represented by triangles; ... µg IL- 1Ra/ ml, and concentrations superior or equal to µg IL- 1Ra/ ml exist only for about 14 hours Local, intraarticular gene delivery of IL- 1Ra could produce enough protein, with only a single...
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Characterization of Fabry mice treated with recombinant adeno-associated virus 2/8-mediated gene transfer pdf
... kidneys of wild-type mice were strongly labeled with α-Gal A, and most staining was observed in tubular epithelial cells (Fig 3A) Glomerular cells including podocytes and mesangial cells did not ... proteinuria and glomerulosclerosis can develop In addition, in the inner capillaries, the pores of endothelial cells underwent fenestration and formed an inclusion The mesangial cells became complex and ... previous study indicated that although the glycosphingolipids may accumulate in endothelial, glomerular, and tubular cells in Fabry disease, glomeruli and endothelial cells did not express the enzyme...
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Báo cáo y học: "Acetyl salicylic acid augments functional recovery following sciatic nerve crush in mice" pot
... nerve regeneration [5] Non-steroidal anti-inflammatory agent acetyl salicylic acid (ASA), in addition to its well known inhibitory action on cyclooxygenases, affects several cellular signalling pathways ... withdrawal reflex of the hind limb and vocalization in response to noxious stimulation like mechanical stimulation (pinch test) and pricking the plantar aspect of the lateral part of the foot with ... peripheral regeneration FEBS Journal 2005, 272:2628-38 Shiff SJ, Qiao L, Tsai L- L, Rigas B: Sulindac Sulfide, an Aspirin-like Compound, Inhibits Proliferation, Causes Cell Cycle Quiescence, and...
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Báo cáo y học: " Effect of oat bran on time to exhaustion, glycogen content and serum cytokine profile following exhaustive exercise" pdf
... Catholic University of Brasilia, Brasília/DF/Brazil 3Molecular Biology of the Cell Group, Institute of Biomedical Sciences, Department of Cell and Developmental Biology, University of São Paulo, ... glycogen in small tissue samples Journal of Applied Physiology 1970, 28(2):234-236 Sambrook J, Russell DW: Molecular cloning: A laboratory manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, ... minutes), the IL-6 has a close relationship with the amount of muscle glycogen and regulation of the homeostasis of blood glucose during long duration exercises Muscular glycogen and blood glucose are...
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