isolation of muscle stem cells by fluorescence activated cell sorting cytometry
Báo cáo khoa học: Death-associated protein kinase (DAPK) and signal transduction: fine-tuning of autophagy in Caenorhabditis elegans homeostasis doc
... necrotic cell death is characterized by rapid loss of plasma membrane integrity, cellular swelling and subsequent release of internal contents [48] In C elegans, gain -of- function mutations of mec-4, ... phosphatase that couples calcium signals to changes in gene transcription by regulating the nuclear factor of activated T cells family of transcription factors [59] Recently, it has been shown that in ... autophagy by atg gene RNAi decreases the survival of worms during starvation, and that the survival can be rescued by the addition of food Autophagy-deficient worms have decreased activity of the...
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Báo cáo sinh học: "Regulation of metabolism in Caenorhabditis elegans longevity" ppsx
... elegans (where adult cell and tissue replace ment does not occur) from those in higher eukaryotes, where compromised cells can be eliminated by apoptosis and replaced C elegans cells might have ... downregulation of TOR Mutation of DAF-15/RAPTOR, an activator of TOR, results in increased C elegans life span [13] Interestingly, daf-15 is directly regulated by DAF-16, the ultimate effector of the ... genetically (by eat mutations), or by dilution of nutrients, or by exposure to axenic medium (medium that supports growth without bacteria), actually had increased metabolic rates, as measured by oxygen...
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Báo cáo sinh học: "Robust and specific inhibition of microRNAs in Caenorhabditis elegans" doc
... respective loss of function mutant strains of lin-4 and let-7 For example, progeny of animals injected with antisense oligonucleotides against lin-4 show delayed exit in differentiation of seam cells and ... phenotype with lack of alae (longitudinal ridge) formation in 100% of animals, consistent with the phenotype shown by let-7 knockout mutant animals [5] By contrast, progeny of animals treated ... water and shows increased cellular uptake and availability The authors also modified these oligonucleotides by conjugating one to three molecules of rhodamine per molecule of dextran These modified...
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Báo cáo khoa học: Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-fluorouracil in Caenorhabditis elegans docx
... separated by TLC All assay mixtures were spotted onto PEI cellulose sheets with lL of nonradioactive tracer (100 lg of 5-FU and 100 lg of uridine for the UP assay mixture; 100 lg of 5-FU and 100 lg of ... used to study this enzyme Disruption of UP activity in mouse embryonic stem cells leads to increased 5-FU concentrations in plasma and reduced incorporation of 5-FU into nucleic acids [18] Moreover, ... [8] The concentration of uridine in plasma and tissues is tightly regulated by cellular transport mechanisms and by UP activity [7] UP catalyzes the reversible phosphorolysis of uridine, yielding...
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Báo cáo khoa học: Stage-specific activation of MIG-17⁄ADAMTS controls cell migration in Caenorhabditis elegans docx
... proform and the activated form, was immunoblotted (right lane) Single asterisk, proform of MIG17–GFP; double asterisk, activated form of MIG-17–GFP; arrow, proform of MIG-17– 6His; arrowhead, activated ... leader cells migrate Consistent with these results, activation of MIG-17 was extensive in the basement membranes of muscle, intestine and gonad in L3 Although MIG-17 is secreted from muscle cells of ... of body wall muscle cells (E) Coimmunostaining of anti-prodomain (pink) and Monoclonal (green) Overlap of the two antibody stains appears white (F) Schematic presentation of a cross-section of...
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Báo cáo sinh học: "A global analysis of genetic interactions in Caenorhabditis elegans" doc
... variant by dividing the number of co-classified interacting pairs by the number of all possible coclassified pairs within the variant Similarly, we calculated ‘precision’ by dividing the number of ... subnetwork by RNAi in the presence of Nile Red and measured the accumulation of Nile Red Journal of Biology 2007, 6:8 http://jbiol.com/content/6/3/8 Journal of Biology 2007, (a) Volume 6, Article Byrne ... system In either case, a better understanding of biological systems may be achieved by investigating the entirety of superimposed networks and not just multiply supported links Three lines of...
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Báo cáo y học: "Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails" ppsx
... analysis culture of their embryonic precursors followed by selection of the cells by flow cytometry [18] Although embryonic cell cultures allow the collection of cells at early stages of development, ... instead of FITC Forty-six wild type and 56 che-2::PABP worms at L4 to young adult were observed Cells were identified by their positions and the percentage of stained cells was scored Responses of ... contrast (DIC) -fluorescence microscope Cells were identified according to their positions [54] by comparing the fluorescence images of GFP and DiQ staining with Nomarski images of the same animal...
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Báo cáo y học: "Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution" pdf
... simultaneous targeting of both genes of a duplicate pair Quantitative analysis of page) Quantitative analysis of synthetic phenotypes following the simultaneous targeting of both genes of a duplicate ... with a simple model of redundancy, suggesting that the function of a single gene identified in one organism is a good predictor of the redundant function covered by a pair of duplicated genes ... this time period in the context of the rate of change of coding genes, C elegans and C briggsae only share about 60% of their genes as 1:1 orthologs, and a full 10% of genes encoded in either genome...
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Báo cáo y học: "Identification of ciliary and ciliopathy genes in Caenorhabditis elegans through comparative genomics" pptx
... subcellular organelle that projects from the surface of many eukaryotic cells in vertebrates, including kidney and endothelial cells, myocardial cells, odontoblasts, retinal photoreceptor cells ... 1.5-fold or greater level of expression in the ciliated subset of neuronal cells versus predominantly non-ciliated cell subsets (that is, pan-neuronal, intestinal and muscle cell subsets) Combining ... phasmid Cell body Dendrite Dendrite Cell body Dendrite Cell body F32A6.2 - amphid Cell body F32A6.2 - phasmid Dendrite Cell body F41E7.9 - phasmid Dendrite Cell body Dendrite F41E7.9 - amphid Cell...
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Báo cáo khoa học: Cyclic ADP-ribose requires CD38 to regulate the release of ATP in visceral smooth muscle ppt
... spontaneous release of ATP but not the release of ATP evoked by action potential firings The enhancing effect of cADPR on spontaneous release of ATP was: (a) unaffected by inhibition of P2 purinoreceptors; ... the bladder was activated by stimulation of intracellular cADPR receptors with cADPR (discussed below) The spontaneous release of ATP also tended to be reduced by inhibition of ryanodine receptor ... potential-evoked release of ATP Therefore, the neuromodulator effects of cADPR are not mediated by influx of extracellular Ca2+, but are probably caused by Ca2+ release from intracellular stores Similar...
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Báo cáo Y học: Dystrobrevin requires a dystrophin-binding domain to function in Caenorhabditis elegans doc
... action of BAL31 was checked by loading an aliquot of each tube onto an agarose gel column The DNA corresponding to the deletions required was treated by applying T4 DNA polymerase in the presence of ... protein and the various forms of the AD-Dyb-1 fusion proteins were analysed on the basis of transactivation of the HIS3 reporter gene after days of growth on SD medium devoid of tryptophan, leucin and ... helix of the Dyb-1 coiled-coil domain strongly decrease or abolish the functional properties of Dyb-1 DISCUSSION The aim of this study was twofold: first, to confirm and refine the localization of...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
... structure of the plasma membrane of E coli cells by a nonstereospecific process was also confirmed by employing calcein-loaded liposomes made of PE ⁄ PG (7 : 3, w:w) Different concentrations of peptide ... (µM) % of lactamase release B 100 80 60 40 20 10 32 15 20 Time (min) Fig Permeation of E coli OM by Esc(1–18) (A) Effects of different concentrations of Esc(1–18) on permeation of the OM of E coli ... perturbation of the OM, and the greatest perturbation was obtained at the MIC of the peptide (32 lm with · 107 CFUÆmL)1) The rate of CENTA hydrolysis, upon addition of · MIC of Esc(1–18) to the cells, ...
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Báo cáo khoa học: Lysosomal localization of GLUT8 in the testis – the EXXXLL motif of GLUT8 is sufficient for its intracellular sorting via AP1- and AP2-mediated interaction docx
... Localization of GLUT8 is not altered in cells lacking AP3 subunits (mocha and pearl cells) In order to confirm our biochemical data, we investigated the subcellular localization of GLUT8 in living cells ... wild-type (E) as well as mutant (K) cells Scale bars = 10 lm or clathrin heavy chain (CHC) in Hela cells stably expressing GLUT8 by siRNA Intracellular targeting of surface accessible GLUT8 was then ... to (a) identify the subcellular localization of GLUT8 in testis, (b) elucidate the role of APs in GLUT8 sorting, and (c) understand the role of the EXXXLL motif in GLUT8 sorting The data provide...
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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... but, as a preliminary step, they unravel one of the mechanisms of TNFamediated withdrawal of insulin action in HepG2 cells Results Incubation of HepG2 cells with TNFa attenuates insulin-stimulated ... role of Foxa2 only, if any, on these series of events There was no significant alteration in Foxa2 localization in cells treated with TNFa alone relative to cells incubated in the absence of any of ... excluded from the nucleus by treatment with insulin, it was strongly detected in the cytosol of insulin-treated cells, but was largely absent in control cells However, when cells were pretreated...
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Báo cáo khoa học: Glutathione transferases kappa 1 and kappa 2 localize in peroxisomes and mitochondria, respectively, and are involved in lipid metabolism and respiration in Caenorhabditis elegans pot
... shown by immunostaining for catalase [14] and by electron microscopy [15] Interestingly, GSTK2::GFP is predominantly expressed in muscle cells (pharynx and body wall muscle) The expression of GST ... muscle cells, GSTK-2::GFP is detected as a tubular network with stronger punctata (arrows) typical of the mitochondrial system Scale bar, 10 lm Expression was increased during morphogenesis of ... number of offspring (250–300 progeny); (d) growth on a solid surface medium; and (e) transparent body allowing the observation of cells in mature and developing animals Furthermore, as about 60% of...
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Báo cáo khoa học: The astacin protein family in Caenorhabditis elegans docx
... these features By contrast, NAS-7::GFP staining was observed only in the head of adult hermaphrodites, but not within pharynx cells (Fig 1C) The expressing cells are located outside of the pharynx, ... pharynx, around the metacarpus and the terminal bulb, and could include neurons, cells of the excretory system or gland cells of still unknown functions [20] Reporter gene expression also became detectable ... magnification of the head of an adult hermaphrodite showing GFP expression for the same construct in pharynx cells and in the lumen of the terminal bulb; 400 · magnification (C) GFP expression of a NAS-7::GFP...
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Báo cáo khoa học: Identification and localization of glycine in the inner core lipopolysaccharide of Neisseria meningitidis ppt
... p.p.m consistent with the presence of a substituent at the 7-position of HepII Signals indicative of the presence and absence of substituents at the 7-position of HepII in the L4 galE oligosaccharide, ... hydrolysis L3 galE core oligosaccharide consisted of two sets of ions differing by 57 a.m.u (Fig 1A) The ion at m/z 1110 corresponds to a composition of Hex, 2Hep, HexNAc, PEtn, Kdo as would be expected ... be formed (Fig 2) Fragmentation of the positively charged ion at m/z 1169 of L3 galE oligosaccharide (Fig 2A) produced a series of ions due to consecutive losses of glycose residues as indicated...
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A pathway that links reproductive status to lifespan in Caenorhabditis elegans pptx
... germline stem cells to exit mitosis and enter meiosis Lifespan can also be extended when the germline stem cells are forced into meiosis during adulthood.14 At this time, the animals contain germ cells ... shown) In addition, the germline contains cells arrested in meiosis (blue circles) and proliferating germline stem cells (yellow circles) When the germline stem cells are forced into meiosis during ... York Academy of Sciences 161 Control of aging by reproductive tissues Kenyon 14 Arantes-Oliveira, N., J Apfeld, A Dillin & C Kenyon 2002 Regulation of life-span by germ-line stem cells in Caenorhabditis...
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Báo cáo sinh học: "The short coiled-coil domain-containing protein UNC-69 cooperates with UNC-76 to regulate axonal outgrowth and normal presynaptic organization in Caenorhabditis elegans" ppt
... LexA-UNC-119 and GAD-ARL-3 or GAD-UNC-76␥ Cells (3×104) were plated onto +His or -His plates, and serial tenfold dilutions of cells were then subsequently plated Cells were grown at 30°C for 48 h before ... HSN, ALM, PLM, AVM, PVM, BDU, and SDQR, as evidenced by its localization to the cell bodies of these neurons Expression of unc-69 in these latter cells Table Axon outgrowth and guidance defects in ... Association of the UNC-69-UNC-76 protein complex with the tail of KHC could either provide additional levels of regulation, or allow intracellular trafficking of a different repertoire of cargos...
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Báo cáo khoa học: "Translocational changes of localization of synapsin in axonal sprouts of regenerating rat sciatic nerves after ligation crush injury" pptx
... I Its general distribution in synapses of the central and peripheral nervous system demonstrated by immunofluorescence in frozen and plastic sections J Cell Biol 1983a, 96, 13371354 17 De Camilli ... the connective tissues of peripheral nerves J Anat 1968, 103(2), 233-243 22 Han H Q., Greengard P Remodeling of cytoskeletal architecture of nonneuronal cells induced by Synapsin Proc Natl Acad ... transported distribution of SyI [13] In the kinetics of the axonal transport, three pools of SyI present biochemically The first pool of newly synthesized SyI departs from the cell body immediately...
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